Agricultural and management practices and bacterial contamination in greenhouse versus open field lettuce production.

The aim of this study was to gain insight into potential differences in risk factors for microbial contamination in greenhouse versus open field lettuce production. Information was collected on sources, testing, and monitoring and if applicable, treatment of irrigation and harvest rinsing water. These data were combined with results of analysis on the levels of Escherichia coli as a fecal indicator organism and the presence of enteric bacterial pathogens on both lettuce crops and environmental samples. Enterohemorragic Escherichia coli (EHEC) PCR signals (vt1 or vt2 positive and eae positive), Campylobacter spp., and Salmonella spp. isolates were more often obtained from irrigation water sampled from open field farms (21/45, 46.7%) versus from greenhouse production (9/75, 12.0%). The open field production was shown to be more prone to fecal contamination as the number of lettuce samples and irrigation water with elevated E. coli was significantly higher. Farmers comply with generic guidelines on good agricultural practices available at the national level, but monitoring of microbial quality, and if applicable appropriateness of water treatment, or water used for irrigation or at harvest is restricted. These results indicate the need for further elaboration of specific guidelines and control measures for leafy greens with regard to microbial hazards.

Relationships among hygiene indicators and enteric pathogens in irrigation water, soil and lettuce and the impact of climatic conditions on contamination in the lettuce primary production.

Eight Belgian lettuce farms located in the West Flanders were sampled to establish the relationships between levels of indicator bacteria, detection of enteric zoonotic pathogens and the temperature and precipitation during primary production. Pathogenic bacteria (PCR EHEC positives, Salmonella spp. or Campylobacter spp.) and indicator bacteria (total psychrotrophic aerobic plate count (TPAC), total coliforms, Escherichia coli, enterococci) were determined over a period of one and a half year from seedling leaves, peat-soil of the seedling, lettuce crops, field soil and irrigation water. Neither Salmonella isolates nor PCR EHEC signals were detected from lettuce although one out of 92 field soil samples contained Salmonella spp. and five soil samples provided PCR positives for EHEC virulence factors (vt1 or vt2 and eae gene). A low prevalence of Campylobacter (8/88) was noted in lettuce. It was shown that irrigation water is a major risk factor with regard to the bacterial contamination of the fresh produce as the water samples showed on a regular basis E. coli presence (59.2% of samples≥1CFU/100ml) and occasionally detection of pathogens (25%, n=30/120), in particular Campylobacter spp. The highest correlations between indicator bacteria, pathogens, temperature and the amount of precipitation were observed for the water samples in contrast to the soil or lettuce samples where no correlations were observed. The high correlations between E. coli, total coliforms and enterococci in the water implicated redundancy between analyses. Presence of elevated levels of E. coli increased the probability for the presence of pathogens (Campylobacter spp., EHEC and Salmonella spp.), but had a low to moderate predictive value on the actual presence of pathogens. The presence of pathogens and indicator bacteria in the water samples showed a seasonal effect as they tend to be more present during the months with higher temperature.