Circulation of foot-and-mouth disease serotypes, risk factors, and their effect on hematological and biochemical profiles among cattle and buffalo in Quetta, Balochistan, Pakistan.

Background and Aim: Foot-and-mouth disease (FMD) is an infectious disease of cloven-hoofed animals, including buffalo, cattle, sheep, goats, and pigs, causing major economic losses to the local farmers and, overall, to the national economy of the country. This study aimed to detect FMDV serotypes in year-round FMD outbreaks, hematological and biochemical changes, and oxidative stress in FMDV-infected cattle and buffaloes in the district of Quetta, Balochistan, Pakistan, and the socioeconomic impact of FMD outbreaks on farmers. Materials and Methods: We conducted a cross-sectional study in the district of Quetta, Balochistan, Pakistan, where FMD virus (FMDV) serotypes were detected by enzyme-linked immunosorbent assay (ELISA). Hematological, biochemical, and oxidative analyses were performed by analyzing the blood of FMDV-infected and non-infected animals. Information on the associated risk factors was obtained through a structured questionnaire by interviewing farmers in each FMD-affected farm. Results: Thirty-four out of 38 farms (89%, 95% confidence interval [CI]: 75%-97%) were positive for FMD by ELISA. Higher FMD infection was detected in farms with a herd size of <50 animals (50%, 17/34), followed by >100 animals (32%, 11/34) and 51-100 animals (18%, 6/34). Fifty-seven percent (114/200, 95% CI: 50%-64%) of animals were positive for FMD. Of these, 61% (69/114) were cattle and 39% (45/114) were buffalo. FMD positivity was higher in females (86%, 98/114) than in males (14%, 16/114) and higher in animals older than 2 years of age (52%, 59/114). On average, farmers lose U.S. dollars 3000 annually due to FMD outbreaks. Animals infected with FMDV had significantly (p ≤ 0.05) white blood cell counts and significantly (p ≤ 0.05) lower hemoglobin and total protein concentrations in buffalo and cattle, whereas infected cattle showed significantly (p ≤ 0.05) lower albumin levels. Globulin levels were lower in buffaloes infected. Alanine aminotransferase levels were lower in infected cattle (p ≤ 0.05). Creatinine levels were higher in infected buffalo (p ≤ 0.05). Urea and phosphorus concentrations were higher in FMDV-infected cattle and buffalo (p ≤ 0.05). Calcium levels were lower in infected cattle and buffalo (p ≤ 0.05). Catalase enzyme activity in infected cattle and buffaloes was significantly lower (p < 0.05). Lipid peroxidation was significantly higher in FMDV-infected cattle and buffalo (p ≤ 0.05). Conclusion: This study confirmed serotype O circulation among cattle and buffalo in year-long FMD outbreaks in the Quetta District of Balochistan. Blood analysis identified a parameter deviated from the normal level due to FMDV infection. In addition, the outbreak of FMD has a significant negative economic impact on livestock farmers.

Effect of Silymarin as an Adjunct Therapy in Combination with Sofosbuvir and Ribavirin in Hepatitis C Patients: A Miniature Clinical Trial.

Silymarin is proclaimed to be a blend of flavonolignans or phytochemicals. An era of new generation of direct-acting antivirals (DAAs) has commenced to have facet effect in swaying of the hepatitis C virus (HCV). Nonetheless, this therapy has serious side effects that jeopardize its efficacy. This study is aimed at probing the effects of ribavirin (RBV) and sofosbuvir (SOF) along with silymarin as an adjunct therapy on hematological parameters and markers of obscured oxidative stress. The effect of DAAs along with silymarin was also examined on variable sex hormone level and liver function markers such as alanine aminotransferase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), and bilirubin. The study was followed to determine viral load and viral genotypes. A total of 30 patients were randomly divided into two equal groups comprising the control group (n = 15) and treatment group (n = 15). The control group was solely administered with DAAs (SOF and RBV; 400 mg/800 mg each/day). Conversely, the treatment group was dispensed with DAAs, but with adjunct therapy of silymarin (400 mg/day) along with DAAs (400/800 mg/day) over period of 8 weeks. Sampling of blood was performed at pre- and posttreatment levels for the evaluation of different propound parameters. Our data showed that silymarin adjunct therapy enhances the efficiency of DAAs. A decrease in menace level of liver markers such as ALT, ALP, AST, and bilirubin was observed (p > 0.05). The adjunct therapy concurrently also demonstrated an ameliorative effect on hematological indices and oxidative markers, for instance, SOD, TAS, GSH, GSSG, and MDA (p < 0.05), diminishing latent viral load. The silymarin administration was also found to revamp the fluster level of sex hormones. Our outcomes provide evidence that systematic administration of silymarin effectively remits deviant levels of hematological, serological, hormonal, and antioxidant markers. This demonstrates a possibly unique role of silymarin in mitigating hepatitis C.

Iron-Deficiency Anemia in Women of Reproductive Age in Urban Areas of Quetta District, Pakistan.

Anemia is associated with poor health outcomes, and the prevalence of anemia is a significant public health indicator for both developed and developing countries. Iron-deficiency anemia (IDA) is the most common type of anemia, which often develops during pregnancy. A cross-sectional study was conducted in the urban areas of Quetta city among the women of reproductive age (15-49 years) to update the status of IDA in the region. The study participants (n = 216) were selected on a random basis, and the samples were further distributed by age. Overall, 75% of females were nonanemic, and among those that were anemic, 2% were severe, 13% were moderately, and 10% were mildly anemic. Among the IDA-affected women, 83% were non-pregnant. Age-wise distribution of IDA revealed no significant difference among different age groups, but numerically higher observations were recorded in the age groups of less than 30 years. The highest number of moderately IDA-affected women (15%) were in the age group 15-19 with the following IDA indicating parameters: hemoglobin 9.64 g/dl, mean corpuscular volume 63.11 fl, mean corpuscular hemoglobin 20.40%, red cell distribution 19.28%. This study will be beneficial for illustrating the requirement and the development of a program to raise extended awareness in the Quetta communities to overcome the negative health effects of IDA on the female population.

Use of multivariate adaptive regression splines for prediction of body weight from body measurements in Marecha (Camelus dromedaries) camels in Pakistan.

The aim of the present work was to predict live body weight by means of some body measurements, i.e., SH, CG, and BG in indigenous Marecha camel breed. For this purpose, multivariate adaptive regression splines (MARS) algorithm was used at proportions of various training and test sets, i.e., 65:35, 70:30, and 80:20 in V-tenfold cross-validation. In prediction of live body weight of the Marecha camels (160 female and 145 male animals) in the MARS predictive models, pairs of sex-SH (model 1), sex-CG (model 2), and sex-BG (model 3) as potential predictors. The best MARS model in LW prediction was obtained using sex and SH independent variables for 80:20 training and test set. Sex was determined to be an important source of variation in SH, CG, and BG as a result of sexual dimorphism in camels (P < 0.01). MARS results indicated that SH could be used as an indirect selection criterion to obtain elite camel herds on LW of Marecha camels. If genetically confirmed, the Marecha camels whose SH is taller than 165.1 cm could be selected for providing genetic progress in LW. In conclusion, use of MARS algorithm may be worthy of consideration for better identification of camel breed standards and selection of superior Marecha camels for meat productivity in Pakistan.

Use of MARS algorithm for predicting mature weight of different camel (Camelus dromedarius) breeds reared in Pakistan and morphological characterization via cluster analysis.

Mature weight is a significant trait that can be influenced by age, sex, breed, production system, and climate conditions in camels. In camel breeding, it is essential to describe breed standards of the studied camel breeds as part of morphological characterization and to determine morphological traits positively influencing mature weight within the scope of indirect selection criteria. This study was to find the best one among candidate models in prediction of mature weight from several morphological traits measured for eight camel breeds (Bravhi, Kachi, Kharani, Kohi, Lassi, Makrani, Pishin, and Rodbari) raised under Pakistan conditions. The morphological measurements taken from the camels in the study were birth weight (BW), weaning weight (WW), mature weight (MW), age of ridding (ARD), face length (FL), face width (FW), head length (HL), head width (HW), ear length (EL), ear width (EW), neck length (NL), neck width (NW), hump length (HL), hump width (HuW), heart girth (HG), withers height (WH), body length (BL), fore leg length (FLL), and hind leg length (HLL), respectively. In the prediction of mature body weight as a response variable, the optimal MARS predictive model with 15 terms selected by train function of the caret package produced very high predictive performance without encountering overfitting problem. Goodness of fit criteria were estimated to measure predictive quality of the MARS model using ehaGoF package available in R environment. Morphological characterization of the camel breeds was performed with hierarchical cluster analysis (HCA) on the basis of Euclidean distance-Single linkage. At the first step of hierarchical cluster analysis, the similarity level of Bravhi and Kachi camel breeds was the highest with 85.3569 (%). At the second step, Makrani joined to new cluster of Bravhi and Kachi camels found at the first step, and the similarity level of the new cluster comprising Bravhi, Kachi, and Makrani breeds was found as 84.5562 (%). MW was significantly correlated with BW (0.677), WW (0.536), HL (0.524), HuW (0.529), and ARD (0.375) at P < 0.01, and there was the highest correlation of 0.994 between HHL and FLL (P < 0.01). As a result, it could be suggested that results of MARS modeling may help camel breeders to reproduce the elite camel populations and to describe characteristics associated positively with MW within the scope of indirect selection criteria.