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Osteoarthritis Cartilage ; 23(12): 2158-2166, 2015 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-26067518

RESUMEN

OBJECTIVE: The aim of this work is to establish the human metacarpal as a new whole joint surface early-stage osteoarthritis (OA) model that enables comparisons of articular cartilage and subchondral bone through high resolution contrast-enhanced CT (CECT) imaging, mechanical testing, and biochemical analysis. DESIGN: The fourth metacarpal was obtained from 12 human cadaveric donors and baseline µCT imaging was followed by indentation testing. The samples were then immersed in anionic (Ioxaglate) and cationic (CA4+) iodinated contrast agent solutions followed by CECT. Cartilage GAG content and distribution was measured using the 1,9 dimethylmethylene blue (DMMB) assay and Safranin-O histology staining. Linear regression was performed to compare cartilage and subchondral bone properties. RESULTS: Strong and significant positive correlations were observed between CA4+ CECT attenuation and both GAG content (R(2) = 0.86) and equilibrium modulus (R(2) = 0.84), while correlations using Ioxaglate were insignificant (R(2) ≤ 0.24, P > 0.05). Subchondral bone plate (SBP) thickness negatively and significantly correlated with SBP mineral density (R(2) = 0.49). Cartilage GAG content significantly correlated with several trabecular bone properties, including positive correlations with bone volume fraction (%BV/TV, R(2) = 0.67), trabecular number (Tb.N, R(2) = 0.60), and trabecular thickness (R(2) = 0.42), and negative relationships with structural model index (SMI, R(2) = 0.78) and trabecular spacing (Tb.Sp, R(2) = 0.56). Similarly, equilibrium modulus correlated positively with %BV/TV (R(2) = 0.50), Tb.N (R(2) = 0.59) and negatively with Tb.Sp (R(2) = 0.55) and SMI (R(2) = 0.60). CONCLUSION: This study establishes the human metacarpal as a new early-stage OA model suitable for rapid, high resolution CECT imaging, mechanical testing, and biochemical analysis of the cartilage and subchondral bone, and for examining their inter-relationships.


Asunto(s)
Cartílago Articular/diagnóstico por imagen , Huesos del Metacarpo/diagnóstico por imagen , Articulación Metacarpofalángica/diagnóstico por imagen , Anciano , Anciano de 80 o más Años , Densidad Ósea , Cadáver , Cartílago Articular/metabolismo , Cartílago Articular/patología , Fuerza Compresiva , Medios de Contraste , Femenino , Glicosaminoglicanos/metabolismo , Humanos , Ácido Yoxáglico , Modelos Lineales , Masculino , Huesos del Metacarpo/metabolismo , Huesos del Metacarpo/patología , Articulación Metacarpofalángica/metabolismo , Articulación Metacarpofalángica/patología , Persona de Mediana Edad , Tomografía Computarizada por Rayos X , Microtomografía por Rayos X
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