lncRNA SNHG1 regulates odontogenic differentiation of human dental pulp stem cells via miR-328-3p/Wnt/ß-catenin pathway.

BACKGROUND: Elucidating the mechanism of odontogenic differentiation of human dental pulp stem cells (hDPSCs) is the key to in-depth mastery and development of regenerative endodontic procedures (REPs). In odontogenic differentiation, lncRNAs have a regulatory role. The goal of this research is to determine the involvement of short nucleolar RNA host gene 1 (SNHG1) in hDPSCs' odontogenic differentiation and the mechanism that underpins it. METHODS: hDPSCs were isolated from the dental pulp tissue of healthy immature permanent teeth. Follow-up experiments were performed when the third generation of primary cells were transfected. The proliferation ability was measured by CCK-8. The biological effects of SNHG1 and miR-328-3p were determined by real-time quantitative polymerase chain reaction (qRT-PCR), western blot (WB), alkaline phosphatase (ALP) staining and activity, alizarin red S staining (ARS) and quantification, and immunofluorescence staining. The binding of SNHG1 and miR-328-3p was confirmed using a dual-luciferase reporter assay. qRT-PCR and WB were used to determine whether the canonical Wnt/ß-catenin pathway was activated. RESULTS: On the 0th, 3rd, and 7th days of odontogenic differentiation of hDPSCs, SNHG1 showed a gradual up-regulation trend. SNHG1 overexpression enhanced the mRNA and protein expression of dentin sialophosphoprotein (DSPP), dentine matrix protein 1 (DMP-1) and ALP. We found that SNHG1 could bind to miR-328-3p. miR-328-3p inhibited the odontogenic differentiation of hDPSCs. Therefore, miR-328-3p mimics rescued the effect of SNHG1 overexpression on promoting odontogenic differentiation. In addition, SNHG1 inhibited Wnt/ß-catenin pathway via miR-328-3p in odontogenic differentiation of hDPSCs. CONCLUSION: lncRNA SNHG1 inhibits Wnt/ß-catenin pathway through miR-328-3p and then promotes the odontogenic differentiation of hDPSCs.

Application of the Stratified Nursing Mode of the Prediction Model Constructed Based on Case System Data in the Nursing of Patients with Acute Renal Failure.

Objective: To explore the application of the stratified nursing mode of the prediction model constructed based on case system data in the nursing of patients with acute renal failure (ARF). Methods: A total of 84 patients with ARF confirmed in the hospital were enrolled between February 2020 and February 2022. According to the simple random grouping method, they were divided into an observation group and a control group, 42 cases in each group. The control group was given routine nursing while the observation group was given stratified nursing of the prediction model constructed based on case system data. All were nursed for 2 months. Results: There was no significant difference in general data such as gender, age, body mass index (BMI), serum creatinine (Scr), hemoglobin (Hb), and albumin between the two groups (P > 0.05). Age >60 years, weight fluctuation >2 kg during dialysis, vascular blockage or infection, coronary heart disease, diabetes mellitus, chronic hepatopathy and stroke, bleeding tendency, and neuromuscular abnormalities were high-risk factors for ARF patients, hypertension, thyroid abnormalities, hyperlipidemia, persistent or repeated blood volume overload, and usage of antihypertensive drugs were moderate-risk factors for ARF patients, and nonpermeability dehydration was a low-risk factor of ARF patients. The scores of nursing satisfaction and treatment compliance in the observation group were significantly higher than those in the control group (P < 0.05). After 2 months of nursing, scores of SAS, SDS, and SPBS in both the groups were significantly decreased (P < 0.05), which were significantly lower in the observation group than those in the control group (P < 0.05). Conclusion: The stratified nursing mode of the prediction model constructed based on case system data is conducive to timely and targeted nursing, with high patient satisfaction and cooperation, and a better psychological state.

Oral Health Status of Residents in Jiangsu Province, China: An Epidemiologic Survey.

INTRODUCTION: This study was conducted to characterise the oral health conditions of residents in Jiangsu Province, China. METHODS: In this descriptive study, 5 groups of participants were selected by a multistage stratified equal capacity random sampling, including 3- to 5-year-olds, 12- to 15-year-olds, 35- to 44-year-olds, 55- to 64-year-olds, and 65- to 74-year-olds. The data were collected by using the fifth edition of Oral Health Surveys-Basic Methods recommended by the World Health Organization (WHO). RESULTS: In these 5 groups, the mean DMFT/dmft of coronal caries were 3.07, 0.69, 4.03, 7.15, and 11.97, respectively, resulting in prevalence rates of 59.69%, 34.98%, 52.05%, 56.86%, and 72.19%, respectively. However, the filling rates were low in all age groups (1.88%, 17.53%, 29.88%, 16.13%, and 19.36%, respectively). Only 3.58% of the 12-year-old participants had good pit and fissure sealants. The adults include three groups, 35- to 44-year-olds, 55- to 64-year-olds, and 65- to 74-year-olds, and the prevalence rates of clinical attachment loss (CAL ≥4 mm) were 32.19%, 76.47%, and 85.43%, respectively. The proportions of adult groups with implant dentures, fixed dentures, removable partial dentures, complete dentures, and substandard dentures were 0%, 26.44%, 11.11%, 0.67%, and 4.89%, respectively. Additionally, 49.64% of adults had missing teeth that were not replaced. CONCLUSIONS: Dental caries and periodontal disease were frequent and common in Jiangsu, China. But only a few of the residents sought treatment. Oral diseases continued to be a major problem for local residents.