Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 3 de 3
Filtrar
1.
J Microbiol Immunol Infect ; 51(6): 778-783, 2018 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-28739435

RESUMEN

OBJECTIVE: We investigated the prevalence of Ureaplasma spp. in semen samples of infertile men in Shanghai, China and evaluated the correlation between the sperm parameters (seminal volume, sperm concentration, progressive motility and non-progressive) and the secretary function in these infectious populations. METHODS: Semens were collected from 540 infertile men and 260 fertile control group in shanghai, China and subjected to standard bacterial and Ureaplasma spp. culture. Positive Ureaplasma spp. isolates were further tested by PCR to detect the biovars and serotypes of Ureaplasma spp. Sperm seminological variabilities were analyzed by Computer-Assisted Semen Analysis according to the fifth edition of World Health Organization (WHO) laboratory manual for the examination and processing of human semen. Seminal markers were measured by the automatic analyzer. RESULTS: The prevalence of Ureaplasma spp. in semen specimens was 39.6% (214/540) and 19.2% (50/260) in infertile and control group, respectively. Significant difference was observed between the two groups (P < 0.001). Among all clinical isolates from infertile men (n = 214), 59.3% (n = 127) was Ureaplasma parvum (UPA), 26.2% (n = 56) was Ureaplasma urealyticum (UUR), and 14.5% (n = 31) was mixed species. While those numbers in control group (n = 50) were 64.0% (n = 32), 20.0% (n = 10), 16.0% (n = 8), respectively. There was no significant difference between any two groups (P > 0.05). The progressive motility and the NAG activity of infertile men infected with UPA and mixed species were significantly lower than those of UUR infected subgroup (P < 0.05). CONCLUSIONS: The infection of Ureaplasma spp. plays an important pathogenic role in male infertility. UPA has higher pathogenicity on the progressive motility and the secretary function of epididymis than UUR.


Asunto(s)
Infertilidad Masculina/microbiología , Semen , Infecciones por Ureaplasma/epidemiología , Infecciones por Ureaplasma/microbiología , Ureaplasma/aislamiento & purificación , Adulto , China/epidemiología , ADN Bacteriano/genética , Humanos , Masculino , Persona de Mediana Edad , Reacción en Cadena de la Polimerasa , Prevalencia , Semen/química , Semen/enzimología , Semen/microbiología , Análisis de Semen/normas , Serogrupo , Ureaplasma/clasificación , Ureaplasma/genética , Ureaplasma/patogenicidad , Adulto Joven
2.
J Cell Biochem ; 114(7): 1695-703, 2013 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-23386430

RESUMEN

The F1F0 ATP synthase has recently become the focus of anti-cancer research. It was once thought that ATP synthases were located strictly on the inner mitochondrial membrane; however, in 1994, it was found that some ATP synthases localized to the cell surface. The cell surface ATP synthases are involved in angiogenesis, lipoprotein metabolism, innate immunity, hypertension, the regulation of food intake, and other processes. Inhibitors of this synthase have been reported to be cytotoxic and to induce intracellular acidification. However, the mechanisms by which these effects are mediated and the molecular pathways that are involved remain unclear. In this study, we aimed to determine whether the inhibition of cell proliferation and the induction of cell apoptosis that are induced by inhibitors of the cell surface ATP synthase are associated with intracellular acidification and to investigate the mechanism that underlines the effects of this inhibition, particularly in an acidic tumor environment. We demonstrated that intracellular acidification contributes to the cell proliferation inhibition that is mediated by cell surface ATP synthase inhibitors, but not to the induction of apoptosis. Intracellular acidification is only one of the mechanisms of ecto-ATP synthase-targeted antitumor drugs. We propose that intracellular acidification in combination with the inhibition of cell surface ATP generation induce cell apoptosis after cell surface ATP synthase blocked by its inhibitors. A better understanding of the mechanisms activated by ecto-ATP synthase-targeted cancer therapies may facilitate the development of potent anti-tumor therapies, which target this enzyme and do not exhibit clinical limitations.


Asunto(s)
Adenosina Trifosfatasas/metabolismo , Acidosis/enzimología , Acidosis/metabolismo , Animales , Apoptosis/fisiología , Western Blotting , Células CHO , Línea Celular , Proliferación Celular , Cricetinae , Cricetulus , Técnica del Anticuerpo Fluorescente , Células Hep G2 , Humanos , Concentración de Iones de Hidrógeno , Microscopía Confocal , Células PC12 , Ratas
3.
Zhonghua Nan Ke Xue ; 9(2): 136-9, 2003 Apr.
Artículo en Chino | MEDLINE | ID: mdl-12749137

RESUMEN

Neutrophil Elastase(NE) is a kind of neutral proteinase. Its value in the diagnosis of male genital tract infection has attracted increasing attention in recent years. NE has special diagnostic value in screening the typical genital tract infection caused by Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum and Trichomonas, as well as other kinds of genital infection, such as leukocytospermia and periorchitis. NE has been considered as a specific marker of the male genital tract infection.


Asunto(s)
Enfermedades de los Genitales Masculinos/diagnóstico , Elastasa de Leucocito/sangre , Enfermedades Bacterianas de Transmisión Sexual/diagnóstico , Chlamydia trachomatis , Enfermedades de los Genitales Masculinos/sangre , Enfermedades de los Genitales Masculinos/microbiología , Humanos , Masculino , Neisseria gonorrhoeae , Pronóstico , Enfermedades Bacterianas de Transmisión Sexual/sangre , Enfermedades Bacterianas de Transmisión Sexual/microbiología , Ureaplasma urealyticum
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA