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Eutrophic shallow lakes are generally considered as a contributor to the emission of nitrous oxide (N2O), while regional and global estimates have remained imprecise. This due to a lack of data and insufficient understanding of the multiple contributing factors. This study characterized the spatiotemporal variability in N2O concentrations and N2O diffusive fluxes and the contributing factors in Lake Wuliangsuhai, a typical shallow eutrophic and seasonally frozen lake in Inner Mongolia with cold and arid climate. Dissolved N2O concentrations of the lake exhibited a range of 4.5 to 101.2 nmol/L, displaying significant spatiotemporal variations. The lowest and highest concentrations were measured in summer and winter, respectively. The spatial distribution of N2O flux was consistent with that of N2O concentrations. Additionally, the hotspots of N2O emissions were detected within close to the main inflow of lake. The wide spatial and temporal variation in N2O emissions indicate the complexity and its relative importance of factors influencing emissions. N2O emissions in different lake zones and seasons were regulated by diverse factors. Factors influencing the spatial and temporal distribution of N2O concentrations and fluxes were identified as WT, WD, DO, Chl-a, SD and COD. Interestingly, the same factor demonstrated opposing effects on N2O emission in various seasons or zones. This research improves our understanding of N2O emissions in shallow eutrophic lakes in cold and arid areas.
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Monitoreo del Ambiente , Lagos , Óxido Nitroso , Estaciones del Año , Óxido Nitroso/análisis , Lagos/química , China , Contaminantes Atmosféricos/análisis , Eutrofización , Análisis Espacio-Temporal , Contaminantes Químicos del Agua/análisisRESUMEN
Mercury and arsenic are two highly toxic pollutants, and many researchers have explored the effects of the two substances on the environment. However, the research content of toxic substances in frozen periods is relatively small. To explore the spatial and vertical distribution of mercury and arsenic in the ice, water, and sediments of Wuliangsuhai Lake under ice conditions, and to assess the harm degree of the two toxic substances to human beings. We collected the ice, water, and sediments of the lake in December 2020, and tested the contents of Hg and As. The single-factor pollution index method, the local cumulative index method, and the ecological risk coding method were used to assess the pollution status in these three environmental media, and the Monte Carlo simulation combined with the quantitative model recommended by USEPA was used to assess the population health risk. The results showed that (1) The average single-factor pollution values of Hg and As in water were 0.367 and 0.114, both pollutants were at clean levels during the frozen period. (2) The mean Igeo values of Hg and As were 0.657 and -0.948. The bioavailability of Hg in the sediments of Wuliangsuhai Lake during the frozen period was high, and its average value was 7.8%, which belonged to the low-risk grade. The bioavailability of As ranged from 0.2% to 3.7%, with an average value of 1.3%. (3) Monte Carlo simulation results indicate acceptable levels of health risks in both water and ice. This study preliminarily investigated the distribution characteristics of toxic substances and their potential effects on human health in lakes in cold and arid regions during the frozen period. It not only clarified the pollution characteristics of lakes in cold and arid regions during the frozen period, but also provided beneficial supplements for the ecological protection of lake basins. This study lays a foundation for further environmental science research in the region in the future.
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Microplastics are among the most difficult new pollutants to remove in wastewater treatment plants. In order to explore the occurrence form, size distribution, composition, removal efficiency, migration law, and fate behavior characteristics of microplastic particles in sewage plants, taking a sewage treatment plant in Hohhot as an example, a total of 17 sampling sites were set up. The LAS X software counted the shape, abundance, and size of microplastics and conducted a full-process analysis. The results showed thatï¼ fibrous microplastics had the highest abundance and widest distribution and were the main form of existence, accounting for 61.8% of the total abundanceï¼ the size of microplastics ranged mainly between 0 and 1.00 mm, and among the four sizes, the abundance of microplastics 0.25 to 0.50 mm in China was the highest, accounting for 32.9%. Among the eight types of plastic components detected, polyester substances ï¼PET, PBTï¼, cellulose, and polypropylene ï¼PPï¼ were the main components, accounting for 25%, 21%, and 17%, respectively. The influent abundance of the sewage plant was ï¼73 ±5ï¼ n·L-1, the effluent abundance was ï¼14 ±2ï¼ n·L-1, and the overall removal rate was ï¼80.8 ±12.1ï¼%. Among the three treatment stages of the sewage plant, only the primary treatment played a role in removal, and the abundance of microplastics surged in the secondary treatment. Different structures playing a major role in the removal of microplastics were fine grids ï¼49.2 ±7.4ï¼% and secondary sedimentation tanks ï¼92.4 ±13.9ï¼%. Microplastics mainly existed in the form of fibers, fragments, and films. The proportion of fibers was approximately 70%, and the size of fragments was mainly concentrated between 0.50 and 5.00 mm. Most fragments were in the range of 5.00 mm, accounting for 50%, making them the main form apart from fibrous. The film-like size was mostly concentrated in the range of less than 0.50 mm, accounting for more than 10%. Therefore, improving the removal of small-sized fibrous and film-like microplastics and large-sized fragmented microplastic particles can effectively reduce the pollution risk of microplastics in the environment caused by sewage plant drainage.
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Ciudades , Microplásticos , Eliminación de Residuos Líquidos , Aguas Residuales , Contaminantes Químicos del Agua , Microplásticos/análisis , Eliminación de Residuos Líquidos/métodos , Aguas Residuales/química , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/aislamiento & purificación , China , Aguas del Alcantarillado/química , Plásticos , Tamaño de la Partícula , Polipropilenos , Monitoreo del AmbienteRESUMEN
Water replenishment is an important measure for maintaining and improving the aquatic environmental quality of lakes. The problems of water quality deterioration and water shortage can be alleviated by introducing water of higher quality. However, the mechanism of water replenishment in the improvement of the water quality and trophic status of lakes remains unclear. This study investigated water replenishment in Wuliangsuhai Lake (WLSHL) from 2011 to 2021 by collecting seasonal water samples and conducting laboratory analyses. Water replenishment was found to be capable of significantly improving lake water quality and alleviating eutrophication. It is worth noting that single long-term water replenishment measures have limitations in improving the water quality and trophic status. The whole process was divided into three stages according to the water quality and trophic status, namely the buffer period, decline period, and stable period. During the buffer period, the water quality and trophic status showed only slight improvement because of the small amount of water replenishment and the low proportion of higher-quality water from the Yellow River. In the decline period, with increasing water replenishment, the proportion of higher-quality water from the Yellow River gradually increased, leading to the most significant and stable degree of improvement. In the stable period, increases in the amount of water replenishment had little effect on improving the water quality and trophic status, which is attributable to the balance between internal pollutants (lake water-sediment), and the balance between internal-external pollutants (lake water-irrigation return flow + Yellow River water). On the premise of stable water quality, with eutrophication control as the management goal, the optimal water replenishment would be approximately 10.58 ×108 m3. Further necessary measures for solving aquatic environmental problems include the combination of sediment dredging, optimization of the water replenishment route, and implementation of quality management in water replenishment.
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Monitoreo del Ambiente , Eutrofización , Lagos , Calidad del Agua , Lagos/química , China , Estaciones del Año , Animales , Contaminantes Químicos del Agua/análisis , Abastecimiento de Agua/estadística & datos numéricos , Ríos/químicaRESUMEN
OBJECTIVE: The aim of this study was to investigate the main factors influencing the survival of patients with advanced gastric cancer. METHODS: The clinicopathological data of 120 patients with advanced gastric cancer were analyzed retrospectively, and clinical and pathological data were collected. Tumor tissue staging and grading were re-evaluated, and 5-year overall survival was followed up. The classified data were described by percentages, and the continuous data were described by standard deviations or medians. Univariate analysis was performed using the χ2 test or rank-sum test, followed by Kaplan-Meier survival analysis to calculate the median survival time and 5-year cumulative survival. A multivariate Cox regression model was used to evaluate the independent risk factors affecting survival. The test level was α = 0.05. RESULTS: Patients were followed up for 0 to 60 months, the 5-year overall survival rate was 36.2%, and the median survival time was 53.0 ± 1.461 months. K-M and log-rank test results revealed that tumor location, degree of differentiation, depth of invasion, regional lymph node involvement, and postoperative tumor stage were correlated with a decreased 5-year survival rate (P < 0.05). A multivariate Cox risk regression model was used to analyze the degree of histological differentiation (HR = 1.441; 95% CI = 1.049-1.979; P = 0.024), regional lymph node (HR = 1.626; 95% CI = 1.160-2.279; P = 0.005), and pTNM stage (HR = 2.266; 95% CI = 1.335-3.847; P = 0.002), which are independent risk factors for poor survival. Tumor location (P = 0.191), invasion depth (P = 0.579) and tumor size (P = 0.324) were not found to be independent risk factors. CONCLUSION: The degree of tumor differentiation, regional lymph node metastasis and postoperative pathological stage were found to be independent risk factors for 5-year overall survival in patients with advanced gastric cancer. Standardized and reasonable lymph node dissection and accurate postoperative pathological staging were very important.
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Estadificación de Neoplasias , Neoplasias Gástricas , Humanos , Neoplasias Gástricas/mortalidad , Neoplasias Gástricas/patología , Masculino , Femenino , Persona de Mediana Edad , Estudios Retrospectivos , Anciano , Factores de Riesgo , Adulto , Estimación de Kaplan-Meier , Modelos de Riesgos Proporcionales , Pronóstico , Tasa de Supervivencia , Metástasis Linfática/patologíaRESUMEN
OBJECTIVE: Previous epidemiological and experimental studies have yielded conflicting results regarding the influence of human micronutrient levels on the risk of colorectal polyps (CP). In our study, we conducted a two-sample Mendelian randomization (MR) investigation to probe the link between 13 human micronutrients (calcium, selenium, magnesium, phosphorus, folate, vitamins B-6, B-12, C, D, beta-carotene, iron, zinc, and copper) and the genetic susceptibility to CP. METHODS: Summary statistics for CP (n = 463,010) were obtained from pan-European genome-wide association studies, and instrumental variables for 13 micronutrients were screened from published genome-wide association studies (GWAS). After selecting suitable instrumental variables, we performed a two-sample MR study, deploying sensitivity analyses to judge heterogeneity and pleiotropy, using inverse variance weighted methods as our primary estimation tool. RESULTS: Our study identified that a genetic predisposition to elevated toenail and circulating selenium or serum ß-carotene concentrations lowers the risk of CP occurrence. However, no statistically significant association was observed between the other 11 micronutrients and the risk of CP. CONCLUSION: The study findings provide evidence that the micronutrient selenium and ß-carotene may confer protective effects against the development of CP.
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Pólipos del Colon , Predisposición Genética a la Enfermedad , Estudio de Asociación del Genoma Completo , Análisis de la Aleatorización Mendeliana , Micronutrientes , Selenio , Humanos , Micronutrientes/sangre , Selenio/sangre , Pólipos del Colon/genética , Pólipos del Colon/sangre , beta Caroteno/sangre , Factores de Riesgo , Polimorfismo de Nucleótido Simple , Neoplasias Colorrectales/genética , Neoplasias Colorrectales/sangre , Neoplasias Colorrectales/epidemiologíaRESUMEN
Ecological pollution caused by heavy metals released from sediments is a worldwide concern. However, the effect of changes in sediment speciation on their release of heavy metals has not been adequately reported. In this study, the research focused on Pb and Cr in the ice period of Wuliangsuhai. This study analyzed changes in the sediment speciation of Pb and Cr before and after a release experiment using four risk assessment methods while varying the temperature, pH, and salinity of the water column. The results indicated that the total concentration of Pb ranged from 11.17 to 24.25 mg/kg, while for Cr it ranged from 42.26 to 69.68 mg/kg. Both elements exhibited mild contamination. The release of Pb and Cr from sediments increases with increasing water temperature, mainly due to the conversion of the residual fraction of Pb to the Fe-Mn oxide fraction and Cr converting more residual fraction to the organic matter and sulfide fraction. The release of sediment Pb and Cr decreased with increasing pH, with Pb converting more acid extractable fraction to the residual fraction and Cr converting more organic matter and sulfide fraction to the residual fraction. In contrast, the release of Pb and Cr increased and then decreased with increasing salinity. For Pb, the acid extractable fraction was more susceptible to conversion to the residual fraction by environmental influences, whereas for Cr, the organic matter and sulfide fraction were susceptible to conversion to the residual fraction.
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Metales Pesados , Contaminantes Químicos del Agua , Lagos , Hielo , Plomo , Sedimentos Geológicos , Contaminantes Químicos del Agua/análisis , Monitoreo del Ambiente/métodos , Metales Pesados/análisis , Agua , Medición de Riesgo , Sulfuros , ChinaRESUMEN
To explore the spatial and temporal distribution characteristics of heavy metals in the surface sediments of lakes in cold regions during ice-sealing and non-ice-sealing periods, we analyzed the potential ecological risk degree and the pollution sources. A total of 20 sampling sites in Lake Ulansuhai in cold regions were collected from 2020 to 2021, and 120 surface sediment samples were collected during different periods. The contents of As, Cd, Cr, Cu, Ni, Pb, Zn, and Hg were determined. Correlation analysis, principal component analysis, and the absolute factor score-multiple linear regression (APCS-MLR) receptor model were used to trace the source of heavy metal pollution. The results showed that:â the distribution of heavy metals in lake surface sediments was different between the ice-sealing period and the non-ice-sealing period. The distribution of heavy metals during the ice-sealing period was higher in the north and lower in the south. Cd and Hg were mainly distributed in the central and southern lakes during the non-glacial period. â¡ Hg, Cd, and As were moderately polluted, and Ni was mildly polluted in the surface sediments of the lake. The overall performance was at a high risk level, and the pollution level during the non-ice-sealing period was higher than that during the ice-sealing period. The main environmental risk factors were Hg and Cd, which showed high risk and moderate risk, respectively. ⢠The sources of heavy metals in lake surface sediments were mainly industrial sources from mining and transportation, agricultural sources, and natural sources. As, Ni, Pb, and Hg were mainly affected by industrial sources, with contribution rates of 62.67%, 75.31%, 77.47%, and 80.11%, respectively. The main sources of Cu and Zn were natural sources, and Cd was greatly affected by agricultural sources. The contribution rate was 81.57%. The source of Cr was mainly affected by natural factors, and the influence of human activities and unknown sources require further attention.
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Dyslipidemia has been considered a risk factor for diabetic peripheral neuropathy. Proprotein convertase subtilisin-like/Kexin 9 inhibitor (PCSK9) inhibitors are a new type of lipid-lowering drug currently in clinical use. The role of PCSK9 in diabetic peripheral neuropathy is still unclear. In this study, the effect of alirocumab, a PCSK9 inhibitor, on the sciatic nerve in rats with diabetic peripheral neuropathy and its underlying mechanisms were investigated. The diabetic peripheral neuropathy rat model was established by using a high-fat diet combined with streptozotocin injection, and experimental subjects were divided into normal, diabetic peripheral neuropathy, and alirocumab groups. The results showed that Alirocumab improved nerve conduction, morphological changes, and small fiber deficits in rats with DPN, possibly related to its amelioration of oxidative stress and the inflammatory response.
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Anticuerpos Monoclonales Humanizados , Diabetes Mellitus , Neuropatías Diabéticas , Animales , Ratas , Neuropatías Diabéticas/tratamiento farmacológico , Neuropatías Diabéticas/prevención & control , Inhibidores de PCSK9 , Proproteína Convertasa 9 , Proproteína Convertasas , Nervio Ciático , SubtilisinaRESUMEN
BACKGROUND: Noninvasive fetal RHD genotyping has been provided to nonimmunized RhD-negative pregnant women to guide anti-D prophylaxis. Among the Chinese, more than 30% of the RhD-negative phenotype is associated with variant RHD alleles, which would limit the accuracy of fetal RHD status prediction; thus, more targeting and proper programs need to be developed. STUDY DESIGN AND METHODS: Fluorescence quantitative polymerase chain reaction PCR (qPCR) or Sanger sequencing on all RHD exons was used to detect maternal RHD genotypes. For pregnant women with RHD*01N.01 or RHD*01N.03 alleles, the presence of RHD exons 5 and 10 in cell-free DNA was determined by qPCR. For pregnant women with the RHD(1227G>A) allele, high-throughput sequencing on exon 9 of the RHD gene and RHCE gene was used to predict fetal RhD phenotype. RESULTS: Among 65 cases of Chinese pregnant women with the serologic RhD-negative phenotype, three major genotypes were identified: RHD*01N.01/RHD*01N.01 (61.5%), RHD*01N.01/RHD(1227G>A) or RHD*01N.03/RHD(1227G>A) (20%), and RHD*01N.01/RHD*01N.03 (13.8%), along with three cases of minor genotypes (4.6%). For 43 pregnant women with the RHD*01N.01 or RHD*01N.03 alleles, qPCR on maternal cell-free DNA yielded a 98.5% (42/43) accuracy rate and 100% successful prediction rate. High-throughput sequencing was successfully used to predict fetal RhD phenotypes for 13 pregnant women with RHD(1227G>A). CONCLUSION: On the basis of maternal RHD genotyping, fetal genotyping through qPCR or high-throughput sequencing can improve the accuracy and success rate of prenatal fetal RhD phenotype prediction among Chinese pregnant women. It plays a potential role in guiding anti-D prophylaxis and pregnancy management in Chinese pregnant women.
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Diagnóstico Prenatal , Sistema del Grupo Sanguíneo Rh-Hr , Femenino , Humanos , Embarazo , Ácidos Nucleicos Libres de Células , Pueblos del Este de Asia , Genotipo , Fenotipo , Sistema del Grupo Sanguíneo Rh-Hr/genéticaRESUMEN
This study aimed to understand the occurrence of mercury in the water environment of typical cold and arid lakes and the regulating environmental factors. Water and surface sediment samples were collected from July to August, 2022 in the Wuliangsuhai Lake region for the analysis of total mercury (THg) and total methylmercury (TMeHg). Lake water THg and TMeHg ranged between 19.20 ~ 668.10 and 0.10 ~ 11.40 ng/L, respectively, exceeding China's environmental quality standards and contents of other lakes and reservoirs in China and other areas. Surface sediments showed lower mean THg and TMeHg of 261.85 and 0.18 µg/kg, respectively, with the former significantly exceeding the background value of Inner Mongolia and unpolluted natural lakes but lower than those of lakes affected by human factors, such as aquaculture. Sediments showed relatively low methylation and TMeHg (0.01-0.21%) concentrations. Correlation analysis identified salinity, total dissolved solids, conductivity, and redox potential as important factors affecting mercury speciation in water, whereas those in surface sediments were organic matter, pH, and total iron content. This study conducted preliminary research on the different species of Hg in Wuliangsuhai Lake water environment, which can provide scientific evidence for the specific treatment of Hg pollution in agriculture, or industry and other related fields. Our results suggest that upstream and downstream regulatory agencies should strengthen the regulation of agricultural and industrial production, moderately reduce human activities, and reduce the use of mercury-rich substances such as pesticides.
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Mercurio , Compuestos de Metilmercurio , Contaminantes Químicos del Agua , Humanos , Compuestos de Metilmercurio/análisis , Mercurio/análisis , Lagos/química , Contaminantes Químicos del Agua/análisis , Monitoreo del Ambiente/métodos , Agua/química , China , Sedimentos Geológicos/químicaRESUMEN
Approximately one-third of the year, Daihai Lake experiences freezing conditions. During this period, the primary mechanisms that impact the quality of the lake water quality include the freezing of nutrients by the ice sheet and the migration of nutrients between the ice, water, and sediment. The present investigation involved the collection of samples of ice, water, and sediment, followed by the utilization of the thin film gradient diffusion (DGT) technique to and explore the distribution and migration of diverse nitrogen (N) and phosphorus (P) forms at the interface of ice, water and sediment. The findings indicate that the freezing process led to the precipitation of ice crystals, which in turn caused the migration of a significant proportion (28-64 %) of nutrients to the subglacial water. The predominant constituents of N and P in subglacial water, were NO3--N and PO43--P, which constituted 62.5-72.5 % of total N (TN) and 53.7-69.4 % of total P(TP). Respectively, with increasing depth, the TN and TP of sediment interstitial water increased. The sediment in the lake acted as a source of PO43--P and NO3--N while acting as a sink or NH4+-N. Soluble reactive P (SRP) flux and NO3--N flux were responsible for 76.5 % and 2.5 % of the P and N present in the overlying water. Additionally, it was observed that 60.5 % of the NH4+-N flux in the overlying water was absorbed and subsequently deposited in the sediment. The presence of soluble and active P in the ice sheet could play a crucial role in the regulation of sediment release of both SRP and NH4+-N. Additionally, the presence of high nutritional salts and the concentration of nitrate nitrogen in the overlying water would certainly increase the pressure of the water environment. Endogenous contamination must be urgently controlled.
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Mercury (Hg) poses a significant risk in paddy fields, particularly when it is converted to methylmercury (MeHg) and accumulates in rice. However, the bioavailability and resupply kinetics of Hg in the paddy soil-water environment are not well understood. In this study, the diffusive gradients in thin films (DGT) and the 'DGT-induced fluxes in sediments' model (DIFS) were first adopted to investigate the Hg resupply kinetics, diffusion fluxes and bioavailability in a paddy environment subjected to flood-drain-reflood treatment and straw amendment. Our results show that although the straw amendment limited the bioavailability of Hg (38.2%-47.9% lower than control) in porewater by decreasing its resupply capacity, especially with smaller straw particles, the net production of MeHg in paddy fields was significantly increased after straw amendment (73.5%-77.9% higher than control). The results of microbial sequencing indicate that enhanced methylators (e.g., family Geobacter) and non-Hg methylators (e.g., Methanosarcinaceae) played a crucial role in MeHg production following straw amendment. Moreover, Hg-containing paddy soils generally tend to release Hg into the overlying water, while drain-reflood treatment changes the direction of Hg diffusion fluxes in the paddy soil-water interface. The drainage-reflooded treatment decreases the Hg reactive and resupply capacity of the paddy soil, thereby hindering the release of Hg from soil into overlying water during the early stages of reflooding. Overall, this study provides novel insights into the behavior of Hg in paddy soil-water surface microlayers.
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Mercurio , Compuestos de Metilmercurio , Oryza , Contaminantes del Suelo , Suelo , Disponibilidad Biológica , Inundaciones , Contaminantes del Suelo/análisis , Mercurio/análisisRESUMEN
The transformation of Hg(II) and Hg(0) in aqueous systems governs the speciation and biogeochemical cycling of Hg. However, with the increasing amount of microplastics in the aqueous environment, little is known about the different effects of microplastic particles and their leached DOM on the photoreduction of Hg(II) to Hg(0) after long-term photoaging. In this study, we found that aged microplastic particles significantly inhibited the photoreduction efficiency (6.40-15.64 %) of Hg(II) compared to the control without any microplastic (31.02 %) and pristine microplastic particles (9.95-34.10 %). This inhibition was due to the adsorption of Hg by the microplastic particles, which decreased the amount of available Hg(II) (Hg(II)RED) that could participate in the photoreduction reaction. The characteristics of aged microplastic particles also indicated rougher surfaces and more oxygen-containing functional groups after photoaging, which may enhance their adsorption capacity compared to pristine microplastic. Interestingly, the photoreduction efficiency of Hg(II) was 11.58-53.28 % higher in the presence of microplastic leach DOM compared to the control. With increasing age, the microplastic leach DOM obviously promotes the photoreduction of Hg(II). Free radical inhibition and electron paramagnetic resonance spectra demonstrated that O2- in microplastic leach DOM contributed to the photoreduction of Hg(II) under light irradiation. Moreover, X-ray absorption near edge structure analyses demonstrated that microplastic leach DOM produced Hg(I) as the primary photoproduct, accounting for 43.17 % of the total Hg in the photoreaction solution and likely reducing it to Hg(0). This study provides novel insights into forecasting the synthesized risks of microplastic aging in the biogeochemical cycle of Hg within aqueous environments.
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Glucocorticoids are commonly used in clinic but are also a double-edged sword. While treating tumors, they are reported to promote tumor growth and metastasis. To explore the role and elucidate the mechanism of dexamethasone in promoting tumor growth and metastasis, we detected the levels of cortisol and adrenocorticotropic hormone (ACTH) in peripheral blood of patients with gastric cancer, and immunohistochemical staining was used to detect the expression of ROR1 in the surgically resected gastric cancer samples. The levels of cortisol and ACTH in peripheral blood of patients with stage III and IV gastric cancer were higher than those of patients with stage I/II gastric cancer. Dexamethasone up-regulated the ROR1 level on gastric cancer cell lines in a concentration-dependent manner. Gastric cancer specimen with high ROR1 had higher rates of relapse and metastasis than gastric adenocarcinomas expressing low levels of ROR1.Gastric cancer patients with high expression of ROR1 had a short survival time. ROR1 was expressed by gastric cancer cell lines, but not on normal gastric epithelial cell line. Suppressing ROR1 in gastric cancer cell lines impaired their invasion, migration, scratch healing and clone formation ability in vitro and slowed down the tumor growth of MKN-45 cells in immunodeficient mice in vivo. Collectively, our study indicated that dexamethasone up-regulated ROR1 levels on gastric cancer cells. ROR1 participated in and mediated the role of dexamethasone in promoting gastric tumor growth, and blocking ROR1 can prevent the tumor growth.
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Neoplasias Gástricas , Animales , Ratones , Neoplasias Gástricas/patología , Línea Celular Tumoral , Glucocorticoides , Hidrocortisona , Recurrencia Local de Neoplasia , Proliferación Celular , Hormona Adrenocorticotrópica , Dexametasona , Regulación Neoplásica de la Expresión GénicaRESUMEN
Based on Porter's hypothesis and Pollution Shelter's hypothesis, this paper firstly constructs a mechanism of environmental protection tax law and corporate environmental performance. Secondly, it empirically examines the impact of green tax reform on corporate environmental performance and its internal mechanism through the difference in difference (DID) method. The study findings firstly reveal that environmental protection tax law significantly and progressively promotes the improvement of corporate environmental performance. Secondly, the heterogeneity analysis results show that the promotion effect of environmental protection tax law on corporate environmental performance is significant in enterprises with strong financial constraints and stronger internal transparency. The environmental performance improvement effect of the state-owned enterprises is stronger, which indicates that state-owned enterprises can set an example in the face of the formal implementation of the environmental protection tax law. In addition, the heterogeneity of corporate governance shows that the background of senior executives is an important factor affecting the effect of environmental performance improvement. Thirdly, the mechanism analysis indicates that the environmental protection tax law mainly promotes the improvement of enterprise environmental performance by strengthening the local government's enforcement rigidity, improving the local government's environmental concern, promoting enterprise green innovation, and resolving the collusion between government and enterprise. Further analysis shows that the environmental protection tax law based on the empirical results of this paper did not significantly trigger the phenomenon of cross-regional negative pollution transfer of enterprises. The findings of the study have important enlightenment on improving the green governance of enterprises and promoting the high-quality development of national economy.
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Desarrollo Económico , Organizaciones , Gobierno , Clima , Contaminación Ambiental , China , Conservación de los Recursos NaturalesRESUMEN
The widespread of different NDM variants in clinical Enterobacterales isolates poses a serious public health concern, which requires continuous monitoring. In this study, three E. coli strains carrying two novel blaNDM variants of blaNDM-36, -37 were identified from a patient with refractory urinary tract infection (UTI) in China. We conducted antimicrobial susceptibility testing (AST), enzyme kinetics analysis, conjugation experiment, whole-genome sequencing (WGS), and bioinformatics analysis to characterize the blaNDM-36, -37 enzymes and their carrying strains. The blaNDM-36, -37 harboring E. coli isolates belonged to ST227, O9:H10 serotype and exhibited intermediate or resistance to all ß-lactams tested except aztreonam and aztreonam/avibactam. The genes of blaNDM-36, -37 were located on a conjugative IncHI2-type plasmid. NDM-37 differed from NDM-5 by a single amino acid substitution (His261Tyr). NDM-36 differed from NDM-37 by an additional missense mutation (Ala233Val). NDM-36 had increased hydrolytic activity toward ampicillin and cefotaxime relative to NDM-37 and NDM-5, while NDM-37 and NDM-36 had lower catalytic activity toward imipenem but higher activity against meropenem in comparison to NDM-5. This is the first report of co-occurrence of two novel blaNDM variants in E. coli isolated from the same patient. The work provides insights into the enzymatic function and demonstrates the ongoing evolution of NDM enzymes.
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Infecciones por Escherichia coli , Escherichia coli , Humanos , Infecciones por Escherichia coli/microbiología , Aztreonam/farmacología , beta-Lactamasas/genética , beta-Lactamasas/metabolismo , Plásmidos/genética , Pruebas de Sensibilidad Microbiana , Antibacterianos/farmacología , Antibacterianos/uso terapéuticoRESUMEN
Calorie restriction (CR) and exercise training (EX) are two critical lifestyle interventions for the prevention and treatment of metabolic diseases, such as obesity and diabetes. Brown adipose tissue (BAT) and skeletal muscle are two important organs for the generation of heat. Here, we undertook detailed transcriptional profiling of these two thermogenic tissues from mice treated subjected to CR and/or EX. We found transcriptional reprogramming of BAT and skeletal muscle as a result of CR but little from EX. Consistent with this, CR induced alterations in the expression of genes encoding adipokines and myokines in BAT and skeletal muscle, respectively. Deconvolution analysis showed differences in the subpopulations of myogenic cells, mesothelial cells and endogenic cells in BAT and in the subpopulations of satellite cells, immune cells and endothelial cells in skeletal muscle as a result of CR or EX. NicheNet analysis, exploring potential inter-organ communication, indicated that BAT and skeletal muscle could mutually regulate their fatty acid metabolism and thermogenesis through ligands and receptors. These data comprise an extensive resource for the study of thermogenic tissue molecular responses to CR and/or EX in a healthy state.
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Tejido Adiposo Pardo , Restricción Calórica , Ratones , Animales , Tejido Adiposo Pardo/metabolismo , Células Endoteliales , Transcriptoma , Termogénesis/fisiología , Músculo Esquelético/metabolismo , Metabolismo Energético/fisiologíaRESUMEN
Understanding the spatial distribution and characteristics of microplastics (MPs) in lake waters is essential to assessing and addressing lacustrine MP pollution. This study investigated how lake ice affects the abundance, spatial distribution, and characteristics (size, shape) of MPs in Lake Ulansuhai by analyzing samples collected at ten sites uniformly distributed throughout the lake during ice-free and ice-covered periods. The abundance of MPs ranged between 204 ± 28 and 1224 ± 185 n·L-1 in lake waters during the ice-free period, and from 34 ± 8 to 216 ± 21 n·L-1 and 269 ± 84 to 915 ± 117 n·L-1 in water and ice during the ice-covered period, respectively. During the ice-covered period, MPs were 2.74-8.14 times higher in the ice than in water beneath the ice. Ice formation decreased MP abundance in lake waters, in part, by incorporating a relatively high percentage of MPs into the ice mass during freezing and by inhibiting atmospheric MPs from reaching the lake waters. The abundance of MPs in the water during the ice-free period was 4.50-11.30 times greater than during the ice-covered period. Seasonal variations in MP shape also occurred; the proportion of fibrous MPs in water decreased during the ice-covered period. Variations in MP abundance were partly due to differences in sedimentation rates; the settling of fibrous MPs is slower, making it easier for them to be captured during the formation of surface ice. Spatially, MPs were uniformly distributed during the ice-free period, but exhibited a spatially distinct pattern during ice-covered periods, when MPs in lake waters were higher in the northeast and lower in the southwest portions of the lake. During the ice-free period, small MPs (0.05-0.5 mm) were more likely to move with currents in the lake, whereas water velocities were reduced by ice formation, allowing small MPs to accumulate near the lake inlet.
Asunto(s)
Microplásticos , Contaminantes Químicos del Agua , Plásticos , Lagos , Contaminantes Químicos del Agua/análisis , Monitoreo del Ambiente , AguaRESUMEN
Background/Aims: This study aimed to investigate the biological function and regulatory mechanism of TCN1 in colorectal cancer (CRC). Methods: We studied the biological function of TCN1 by performing gain-of-function and loss-of-function analyses in HCT116 cell lines; examined the effects of TCN1 on the proliferation, apoptosis, and invasion of CRC cells; and determined potential molecular mechanisms using HCT116 and SW480 CRC lines and mouse xenotransplantation models. Tumor xenograft and colonization assays were performed to detect the tumorigenicity and metastatic foci of cells in vivo. Results: TCN1 knockdown attenuated CRC cell proliferation and invasion and promoted cell apoptosis. Overexpression of TCN1 yielded the opposite effects. In addition, TCN1-knockdown HCT116 cells failed to form metastatic foci in the peritoneum after intravenous injection. Molecular mechanism analyses showed that TCN1 interacted with integrin subunit ß4 (ITGB4) to positively regulate the expression of ITGB4. TCN1 knockdown promoted the degradation of ITGB4 and increased the instability of ITGB4 and filamin A. Downregulation of ITGB4 at the protein level resulted in the disassociation of the ITGB4/plectin complex, leading to cytoskeletal damage. Conclusions: TCN1 might play an oncogenic role in CRC by regulating the ITGB4 signaling pathway.