[Astragali Radix and Hedysari Radix molecular identification of SSR primers screening and fingerprints code].

Leguminous related SSR primers were collected, core primers used for Astragali Radix and Hedysari Radix identification were screened and validated by using molecular marker techniques. 6 core primers were selected from 101 pairs of primers, the molecular weight of PCR products was 100-500 bp, which formed 7-12 electrophoresis bands with 55 amplified loci. The percentage of polymorphic loci was 100%, and the average polymorphism information content was 0.371. According to the results of cluster analysis, obtained core primer could completely distinguish 62 mixture samples of Astragali Radix and Hedysari Radix in similarity coefficient of 0.46. Core primers and the corresponding characteristics from gel electrophoresis were tagged. The results provide identification basis for Astragali Radix and Hedysari Radix.

[Genetic Diversity and Cluster Analysis of Astragalus membranaceus in Gansu Province].

Objective: To investigate the genetic status of Astragalus membranaceuse resources in Gansu province. Methods: Using SSR molecular marker technology for collection of Astragalus membranaceuse resources for genetic diversity analysis and clustering analysis. Results: Nine SSR primers were used on PCR amplification of 57 samples in six main areas of Gansu province,PCR products molecular weighted between 100 ~ 500 bp,the polymorphic loci was 82,the polymorphism rate was 97. 56%,and the average polymorphism information content was 0. 438. At the species level,the number of alleles was 1. 976,the effective number of alleles was 1. 459,Nei' s genetic diversity was 0. 279,Shannon information index was 0. 431,the group of genetic diversity degree was 0. 248,the genetic differentiation among of population was 0. 117,the gene flow coefficient was 3. 775,and the genetic identity was 0. 896 ~ 0. 977. Conclusion: Astragalus membranaceuse resources of Gansu are relatively pure,and have abundant genetic diversity. The genetic variation mainly comes from the group of inside,the genes communication between populations is frequent,and the kinship between population is consistent with their geographic distance. In addition,the results of cluster analysis showed that the Core SSR primers can distinguish Astragalus and Hedysarum in the similarity of 0. 46,but Astragalus membranaceus var. mongholicus,Astragalus membranaceus and Astragalus tongolensis can't be distinguished.