RESUMEN
Kawasaki disease (KD) is a childhood-onset vascular disease. We assessed the concentrations of macrophage-colony stimulating factor (M-CSF) and those of lipids in sera from patients with KD. The M-CSF concentration in patients with acute-phase KD was 2,914+/-159 U/ml, significantly higher than that in control subjects with Infectious diseases (1,241+/-96 U/ml). The elevated levels of this cytokine in the acute phase fell to 1,319+/-138 U/ml in the convalescent phase. Total and high-density lipoprotein cholesterol concentrations in acute phase KD (113.8+/-8.4 and 21.5+/-2.3 mg/dl, respectively) were lower than in the infectious disease controls (195.8+/-7.0 and 62.5+/-1.8 mg/dl). The elevation of M-CSF correlated with the decrease of total and high-density lipoprotein cholesterol. Overproduction of macrophage-colony stimulating factor activates macrophages and monocytes and may disturb the lipid metabolism. Both effects could contribute to vasculitis in KD.
Asunto(s)
HDL-Colesterol/sangre , Colesterol/sangre , Factor Estimulante de Colonias de Macrófagos/sangre , Síndrome Mucocutáneo Linfonodular/sangre , Protección a la Infancia , Preescolar , HDL-Colesterol/efectos de los fármacos , Femenino , Humanos , Inmunoglobulinas Intravenosas/uso terapéutico , Lactante , Bienestar del Lactante , Japón/epidemiología , Recuento de Leucocitos , Factor Estimulante de Colonias de Macrófagos/efectos de los fármacos , Masculino , Monocitos/citología , Monocitos/metabolismo , Síndrome Mucocutáneo Linfonodular/tratamiento farmacológico , Estadística como AsuntoRESUMEN
The methanol extract of the dried aerial parts of Prangos tschimganica gave three new coumarin derivatives and 30 known coumarin derivatives. Their structures were established on the basis of chemical and spectroscopic evidence. Absolute configuration of the isolated compounds were determined by using a modified Mosher's method. Some of the isolated compounds showed anti-HIV activity.
Asunto(s)
Fármacos Anti-VIH/química , Apiaceae/química , Cumarinas/química , Furocumarinas/química , Plantas Medicinales/química , Acetilación , Fármacos Anti-VIH/farmacología , Cumarinas/farmacología , Furocumarinas/farmacología , VIH-1/efectos de los fármacos , Espectroscopía de Resonancia Magnética , Conformación Molecular , Espectrometría de Masa Bombardeada por Átomos Veloces , Uzbekistán , Zidovudina/farmacologíaRESUMEN
The methanol extract of the dried aerial parts of Prangos tschimganica afforded seven monoterpenoids and four gamma-pyrone derivatives. Their structures were established on the basis of chemical and spectroscopic evidence.
Asunto(s)
Apiaceae/química , Pironas/aislamiento & purificación , Terpenos/aislamiento & purificación , Estructura Molecular , Pironas/química , Análisis Espectral , Terpenos/químicaRESUMEN
The methanol extract of the dried roots of Rheum maximowiczii afforded four phenylbutanoid and two stilbene derivatives. Their structures were established on the basis of chemical and spectroscopic studies.
Asunto(s)
Butanoles/aislamiento & purificación , Plantas Medicinales/química , Polygonaceae/química , Estilbenos/aislamiento & purificación , Butanoles/química , Espectroscopía de Resonancia Magnética , Extractos Vegetales/química , Raíces de Plantas/química , Espectrometría de Masa Bombardeada por Átomos Veloces , Estilbenos/química , UzbekistánRESUMEN
The effects of roxithromycin, a macrolide antibiotic, on neutrophil activities were investigated in six seriously handicapped patients with severe mental retardation. Neutrophil activities were evaluated by flow cytometry using a heparinized blood analysis method. All six patients showed decreased levels of neutrophil phagocytosis, intracellular killing, and CD11b expression. Treatment with roxithromycin in vitro selectively restored the decreased phagocytic and bactericidal activities of neutrophils in these patients. There was no significant restorative effect with cefaclor, ofloxacin, or aztreonam. These results suggest the need to consider therapeutic effects of antibiotics on neutrophil functions in patients at increased risk for bacterial infections due to decreased neutrophil activities.
Asunto(s)
Antibacterianos/farmacología , Neutrófilos/efectos de los fármacos , Neutrófilos/inmunología , Roxitromicina/farmacología , Adolescente , Adulto , Antiinfecciosos/farmacología , Aztreonam/farmacología , Actividad Bactericida de la Sangre/efectos de los fármacos , Cefaclor/farmacología , Cefalosporinas/farmacología , Niño , Personas con Discapacidad , Interacciones Farmacológicas , Femenino , Citometría de Flujo , Humanos , Peróxido de Hidrógeno/metabolismo , Antígeno de Macrófago-1/biosíntesis , Masculino , Monobactamas/farmacología , Neutrófilos/metabolismo , Ofloxacino/farmacología , Fagocitosis/efectos de los fármacosRESUMEN
Effect of TYB-2285 and its metabolites on immune responses by peripheral blood mononuclear cells (PBMC) from children with bronchial asthma was investigated. TYB-2285 and its metabolites have immunosuppressive activity for the proliferation by Df-stimulated patients' lymphocytes. Concanavalin A (Con A)-activated peripheral blood mononuclear cells (PBMC) from the patients were not affected by the same treatment. The results indicate that TYB-2285 and its metabolites are capable of suppressing antigen-induced 3H-thymidine (3H-TdR) uptake but not the response induced by Con A. Interferon-gamma (IFN-gamma) production by Df-stimulated PBMC from patients with active asthma, which was lower than that of normal lymphocytes, were reversed beyond the levels of that in normal subjects. Thus reduced production of IFN-gamma by Df-stimulated patients' lymphocytes was increased by TYB-2285 and its metabolites in a dose-dependent manner. This phenomenon was not observed in lymphocytes from normal subjects. Furthermore, TYB-2285 inhibited IL-4 production induced by Df antigen in asthmatic patients' lymphocytes. Taken, together, TYB-2285 could work as a weak immunosuppressant to modify lymphocytes' responses to allergen in patients with bronchial asthma. These data underscore the potential benefit for the patients with bronchial asthma.