Análise da imunoexpressão de BAX e MMP-9 no líquen plano oral / Analysis of BAX and MMP-9 immunoexpression in oral lichen planus

Introdução: O líquen plano oral é uma doença crônica imunologicamente mediada relativamente comum, que acomete a mucosa oral. Clinicamente, o LPO é classificado em seis padrões bem identificados: placa, reticular, bolhoso, atrófico, papular e erosivo.Sendo os mais comuns oos tipos reticulares e erosivos. A ativação dos linfócitos TCD4+ no LPO, pode induzir os ceratinócitos ao processo de apoptose através da respostaimunológica citotóxica. A proteína Bax desempenha uma função relevante para o processo apoptótico. Deste modo, a presente pesquisa consistiu em um estudo transversal retrospectivo, descritivo, quantitativo e comparativo. Objetivo: Avaliar a expressão imuno-histoquímica das proteínas MMP9 e Bax no LPO. Método: Foram utilizados 43 casos de LPO para análise da imunoexpressão de Bax e MMP-9. Os resultados foram analisados através dos testes estatísticos apropriados e serão considerados significativos, valores onde p<0,05. Resultado: A imunoexpressão de MMP9 foi significativamente maior nos ceratinócitos e quando analisados os subtipos de líquen plano oral, não foram observados diferenças estatísticas entre os tipos reticulares e erosivos para as proteínas analisadas. Conclusões: Com essas observações, infere-se que a alteração na expressão das proteínas estudadas sugere um distúrbio nos mecanismos apoptóticos, os quais estão associados às lesões de LPO, e podemos concluir também que as imunoexpressões dessas proteínas não apresentaram diferença, quando relacionada ao tipo clínico reticular ou erosivo. Com esse resultado pode-se contribuir para um maior entendimento sobre os possíveis mecanismos celulares envolvidos na etiopatogenia dessa lesão (AU).

Background: Oral lichen planus is a relatively common immune-mediated chronic disease that affects the oral mucosa. Clinically, OLP is classified into six well-identified patterns: plaque, reticular, bullous, atrophic, papular, and erosive. The most common being the reticular and erosive types. The activation of TCD4+ lymphocytes in the OLP can induce keratinocytes to the process of apoptosis through the cytotoxic immune response. Thus, the present research consisted of a retrospective, descriptive, quantitative and comparative crosssectional study. Objective: to evaluate the immunohistochemical expression of MMP-9 and Bax proteins in OLP. Methods: We used 20 cases of Inflammatory Fibrous Hyperplasia as control. The results were analyzed through the appropriate statistical tests and will be considered significant, values where p<0.05. Results: The immunoexpression of MMP-9 was significantly higher in keratinocytes and when the subtypes of oral lichen planus were analyzed, no statistical differences were observed between the reticular and erosive types for the proteins analyzed. Conclusions: With these observations, it is inferred that the alteration in the expression of the studied proteins suggests a disturbance in the proliferative and apoptotic mechanisms, which are associated with a pathological behavior of the oral mucosa, and consequently with a repercussion on the lesions of OLP, and we can also conclude that the immunoexpression of these proteins had no difference, when related to the reticular or erosive clinical type. This research aims to contribute to a greater understanding of the possible cellular mechanisms involved in the etiopathogenesis of this lesion, thus enabling the understanding of the clinical aspects of the pathology (AU).

Pindborg tumor associated with a supernumerary tooth: a case report.

The calcifying epithelial odontogenic tumor is a rare benign neoplasm that accounts for approximately 1% of all odontogenic tumors. Most of the cases occur in the posterior mandible, and a few involve the maxilla. Despite their relatively indolent biological behavior, tumors in the maxilla tend to grow fast. We report the case of a 33-year-old female patient exhibiting swelling in the right maxilla. An isodense area associated with an impacted supernumerary tooth was found on imaging examination. The histopathologic diagnosis was a calcifying epithelial odontogenic tumor. The treatment of choice was surgical removal of the lesion and associated dental elements. The patient has been followed up for 11 months and shows no signs of recurrence. Besides describing this case, we reviewed the literature on the association of calcifying epithelial odontogenic tumors with supernumerary teeth and found two case reports addressing this subject.

Pindborg tumor associated with a supernumerary tooth: a case report

The calcifying epithelial odontogenic tumor is a rare benign neoplasm that accounts for approximately 1% of all odontogenic tumors. Most of the cases occur in the posterior mandible, and a few involve the maxilla. Despite their relatively indolent biological behavior, tumors in the maxilla tend to grow fast. We report the case of a 33-year-old female patient exhibiting swelling in the right maxilla. An isodense area associated with an impacted supernumerary tooth was found on imaging examination. The histopathologic diagnosis was a calcifying epithelial odontogenic tumor. The treatment of choice was surgical removal of the lesion and associated dental elements. The patient has been followed up for 11 months and shows no signs of recurrence. Besides describing this case, we reviewed the literature on the association of calcifying epithelial odontogenic tumors with supernumerary teeth and found two case reports addressing this subject.

Associação de técnicas cirúrgicas periodontais para recobrimento de recessões múltiplas e controle da dor pós operatória com laserterapia: relato de caso / Association of periodontal surgical techniques for covering multiple recessions and control of postoperative pain with lasertherapy: case report

Introdução: As cirurgias periodontais têm sido amplamente utilizadas para a correção de defeitos mucogengivais, como a recessão gengival. Objetivo: relatar por meio de um caso clínico o recobrimento radicular em recessões gengivais classe I de Miller nos dentes 11, 12 e 13 com enxerto de tecido conjuntivo subepitelial associado a retalho posicionado coronalmente. Relato do caso: Paciente do sexo masculino, 24 anos de idade, foi atendido na Clínica de Odontologia da Universidade Federal de Campina Grande, queixando-se de hipersensibilidade nos dentes 11, 12 e 13. Ao realizar a avaliação inicial, as medidas das recessões na face vestibular foram 5mm, 1mm e 2mm, respectivamente. Durante o planejamento periodontal optou-se pela realização dos procedimentos básicos: raspagem e alisamento coronorradicular e orientação de higiene bucal. Em seguida foi necessária a complementação cirúrgica para recobrimento radicular por meio da técnica de enxerto de tecido conjuntivo subepitelial associada ao tracionamento coronal do retalho, a fim de reduzir ou eliminar a recessão e ampliar a faixa de mucosa ceratinizada. Após a cirurgia foi aplicado laser de baixa intensidade a fim de diminuir a dor pós-operatória. O procedimento foi bem sucedido, com a cobertura radicular total. Conclusão: A técnica do enxerto subepitelial de tecido conjuntivo e tracionamento coronal do retalho merecem ser consideradas técnicas efetivas em obter resultados satisfatórios, tendo em vista a possibilidade de recobrimento radicular total em casos de recessões gengivais múltiplas. (AU)

Introduction: Periodontal surgery have been widely used for the correction of mucogengivais defects, such as gingival recession. Objective: To report through a case the root coverage in Miller's Class I recessions on teeth 11, 12 and 13 with graft of subepithelial connective tissue associated with coronally positioned flap. Case Report: A male patient, 24 years old, was treated at the Clinic of Dentistry, Federal University of Campina Grande, complaining of hypersensitivity in the teeth 11, 12 and 13. When performing the initial assessment, the measures of recessions the buccal surface were 5mm, 1mm and 2mm, respectively. During periodontal planning was chosen for carrying out the basic procedures: scaling and root planing and corono-orientation for oral hygiene. Then surgical root coverage for complementation by connective tissue graft technique subepithelial associated flap coronal traction was required in order to reduce or eliminate the recession and extend the range keratinized mucosa. After surgery low intensity laser was applied in to reduce postoperative pain. The procedure was successful, with complete root coverage. Conclusion: The subepithelial graft technique of connective tissue and coronally positioned flap deserve to be considered effective techniques to obtain satisfactory results, given the possibility of complete root coverage in cases of multiple gingival recessions. (AU)