Occlusion effects of bioactive glass and hydroxyapatite on dentinal tubules: a systematic review.

OBJECTIVE: Bioactive glass and hydroxyapatite are biocompatible materials used as an adjunct to various dental materials. The present study aimed to evaluate the occlusion effects of bioactive glasses and hydroxyapatite on dental tubules. MATERIALS AND METHODS: We searched the PubMed/Medline, Embase, and Web of Science databases for the relevant records. The methodological quality of the studies was assessed by an accepted quality assessment tool. RESULTS: From the electronic databases, 372 articles were retrieved. After evaluating the records, 35 in vitro studies were included. The studies revealed a low risk of bias. The primary outcomes from bioactive glass studies demonstrated the potential efficacy of both bioactive glass and hydroxyapatite in dentin tubule occlusion compared to the control. CONCLUSION: The current systematic review showed that bioactive glass and hydroxyapatite could effectively occlude the dentinal tubules. Thus, desensitizing agents containing bioactive glass and hydroxyapatite can be used to manage dentin hypersensitivity (DH). However, long-term follow-up clinical trials are required in the future before definitive recommendations can be made. CLINICAL RELEVANCE: This work achieved a satisfactorily systematic review for assessing desensitizing agents containing bioactive glass and hydroxyapatite in dentine hypersensitivity treatments recommended for clinical practice and research.

The effects of Er:YAG, Nd:YAG, and Diode (940nm) Lasers irradiation on Microtensile bond strength of two steps self-etch adhesives.

BACKGROUND AND AIMS: Nowadays, studies show the effective laser irradiation role on the adhesion of bonding agents to the dentin. Therefore, the present study was set to find an appropriate protocol for the use of Nd:YAG, Er:YAG, and Diode lasers during the application of two steps self-etch adhesives in direct dental restorations in order to achieve higher microtensile strength. MATERIALS AND METHODS: A total number of 100 extracted healthy teeth were selected. After removing occlusal enamel and exposing dentin, samples randomly divided into ten groups: Group Control (C), two steps self-etch adhesives was applied in accordance with the manufacturer instructions, without using laser systems; Groups Er-YAG, laser (2940 nm, 10 Hz, 0.4w, 40 mJ) irradiated Before applying Primer (Er-BP subgroup), After applying Primer (Er-AP subgroup), After applying Bonding (Er-AB subgroup); Groups Nd-YAG, laser (1064 nm, 10 Hz, 1.2w, 40 mJ) irradiated Before applying Primer (Nd-BP subgroup), After applying Primer (Nd-AP subgroup), After applying Bonding (Nd-AB subgroup); Groups Diode, laser (940 nm, 10 Hz, 0.7w, 70 mJ) irradiated Before applying Primer (D-BP subgroup), After applying Primer (D-AP subgroup), After applying Bonding (D-AB subgroup). After intervention and composite build up, the samples were stored 24 hours in 37°C distilled water. The microtensile strength was measured using the universal testing machine. The data were analyzed by one-way ANOVA test and post hoc Tukey test. (p < 0.05). RESULTS: The Means and standard deviations of the groups were as follow: C = 30.09 ± 4.21, Er-BP = 18.83 ± 4.21, Er-AP = 14.43 ± 3.12, Er-AB = 19.67 ± 4.96, Nd-BP = 20.35 ± 5.55, Nd-AP = 39.85 ± 4.13, Nd-AB = 18.16 ± 3.36, D-BP = 26.74 ± 5.05, D-AP = 28.11 ± 5.12, D-AB = 37.28 ± 5.61. The mean microtensile strength achieved in groups: Nd-AP and D-AB were significantly higher than control group (p < 0.05). Groups D-BP and D-AP had no significant difference in comparison with control group while the remaining groups had significantly lower values (P < 0.05). CONCLUSIONS: Nd:YAG laser irradiation after applying the primer, and diode laser irradiation after applying the bonding agent, both can improve the microtensile bond strength in two steps self-etch adhesive systems significantly.

Anti-bacterial effect of essential oil from Xanthium strumarium against shiga toxin-producing Escherichia coli.

Shiga toxin-producing Escherichia coli (STEC) serotype O157:H7 is one of the most important human pathogenic microorganisms, which can cause life-threatening infections. Xanthium strumarium L. is a plant with anti-bacterial activity against gram-negative and gram-positive bacteria. This study aims to demonstrate in vitro efficacy of the essential oil (EO) extracted from Xanthium strumarium L. against E. coli O157:H7. Using the agar test diffusion, the effect of Xanthium strumarium L. EO (5, 10, 15, 30, 60, and 120 mg/mL) was verified at each of the four different growth phases of E. coli O157:H7. Cell counts of viable cells and colony forming unit (CFU) were determined at regular time points using Breed's method and colony counting method, respectively. No viable cell was detectable after the 1 hour-exposure to X. strumarium EO at 30, 60, and 120 mg/mL concentrations. No bacterial colony was formed after 1 h until the end of the incubation period at 24 h. At lower concentrations, the number of bacteria cells decreased and colonies could be observed only after incubation. At the exponential phase, the EO at 15 mg/mL was only bacteriostatic, while from 30 mg/mL started to be bactericidal. X. strumarium EO antibacterial activity against Shiga toxin-producing E. coli O157:H7 is dependent on EO concentration and physiological state of the microorganisms tested. The best inhibitory activity was achieved during the late exponential and the stationary phases.

The comparison of root caries experience between HIV-positive patients and HIV-negative individuals in a selected Iranian population.

OBJECTIVES: Human immunodeficiency virus (HIV) infection is a concerning problem in dentistry and HIV-infected patients may experience root caries due to different risk factors. The aim of this study was to find the prevalence of root caries in a selected Iranian HIV-positive population. METHODS: One hundred and seven IV drug users, based on ELISA and Western Blot test, were divided into two groups: group 1: HIV-positive patients and group 2: HIV-negative individuals. According to the T-CD4(+) cell count, subjects in group 1 were placed in two subgroups: Moderate immunodeficiency (200 mm(-3) < T-CD4(+) cells <500 mm(-3)) and Severe immunodeficiency patients (T-CD4(+) cells <200 mm(-3)). Teeth were examined by an examiner under suitable light to detect any changes in colour, texture or contour. The values of DMFT, DMFS, decayed root surfaces and total decayed surfaces were calculated. Data were analysed by independent t-test and chi-squared test. RESULTS: The mean DMFT, DMFS and decayed root caries in group 1 and 2 had no significant difference. The mean value of total decayed surfaces of HIV+ patients was significantly higher compared with HIV- individuals (P = 0.03). The comparison of all parameters between two subdivisions of group 1 showed no significant difference. CONCLUSIONS: The results indicate that HIV+ patients experienced more dental caries, but not more root caries than healthy ones. Along with decreasing T-CD4(+) cell count, tooth caries' prevalence did not increase. CLINICAL RELEVANCE: Based on our findings, root caries prevalence is almost the same in HIV-positive and negative individuals; however, it is necessary to decrease tooth caries by continual monitoring and periodic dental examination.

Comparison of the effects of two whitening toothpastes on microhardness of the enamel and a microhybride composite resin: an in vitro study.

INTRODUCTION: Whitening toothpastes which have been accepted in populations may affect properties of enamel and restorative materials. The aim of this study was to compare the microhardness of human enamel and Z250 microhybrid composite resin after brushing with two whitening toothpastes. MATERIALS AND METHODS: In this experimental study of enamel specimens, forty five freshly extracted human incisors were prepared and divided into three groups of control enamel (ClE), Crest enamel (CtE) and Aquafresh enamel (AfE). For composite resin specimens, forty five cylindrical-shaped specimens of light-cured Z250 composite were prepared and divided into three groups of control composite (ClC), Crest composite (CtC) and Aquafresh composite (AfC). The control groups were brushed without toothpaste. Crest and Aquafresh group specimens were brushed with Crest and Aquafresh whitening toothpastes, respectively. Vickers microhardness test was performed for all groups. Data were analyzed by One-way ANOVA and Tukey tests. RESULTS: Microhardness values of ClE, CtE, AfE, ClC, CtC and AfC groups were 332.99 ± 26.59, 313.99 ± 20.56, 323.57 ± 27.96, 137.1 ± 3.16, 122.95 ± 3.27 and 130.36 ± 4.8, respectively. One-way ANOVA showed no significant differences among three enamel groups but there was significant difference among composite groups (p<0.01). CONCLUSION: Crest and Aquafresh whitening toothpastes did not affect enamel hardness but reduced the microhardness value of Z-250 composite resin. However, Crest whitening toothpaste decreased the microhardness more than Aquafresh.