Dereplication of calystegines in food plants and wild Solanum Brazilian fruits.

Calystegines are potent glycosidase inhibitors with therapeutic potential and are constituents of food and feed with potential toxic effects. This study aims to target calystegines and other nitrogenous substances in food plants. Hydroalcoholic extracts from Solanum tuberosum, Ipomoea batatas, S. lycocarpum, and fruit from S. lycopersicum, S. aethiopicum, S. paniculatum, S. crinitum, and S. acanthodes were analyzed by liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) using an acidic HILIC column. The dereplication approach included data processing using MZMine2, FBMN-GNPS, and structure elucidation and interpretation of the organized data. The calystegines A3, A5, B2, and C1 were identified, and several potential new calystegine analogues: three may correspond to new calystegines of the A-group, one glycosyl derivative of calystegine A3, and two glycosyl derivatives of the B-group. These findings help to direct the search for new calystegines. In addition, the dereplication approach enabled the annotation of 22 other nitrogen compounds.

Invited Discussion on: Global Prevalence and Preferences of Progressive Tension Suture Usage in Abdominoplasties.

The effectiveness of using quilting/progressive tension sutures in reducing seroma formation after abdominoplasties is well established in the literature, eliminating the use of drains regardless of the simultaneous performance of liposuction. However, despite being widely studied and advocated, the quilting/progressive tension sutures technique is still not widespread, and most surgeons still insist on the use of drains. The author provides a brief literature review on this subject and comments on the work carried out by Wen et al., analyzing divergences between literature data and those presented by Wen et al., and trying to identify the causes of low adherence to the evaluated technique.

Invited Discussion on: The Impact of Reconstructive Modality and Postoperative Complications on Decision Regret and Patient-Reported Outcomes following Breast Reconstruction.

One in eight women will have a lifetime diagnosis of confirmed breast cancer, and one in three of these women will undergo a mastectomy. About half of women undergoing mastectomies will opt for some type of breast reconstruction. Breast cancer itself and breast reconstruction bring physical changes that are accompanied by psychological changes of varying degrees. The decision process about cancer treatment and whether or not to perform breast reconstruction brings suffering. In the case of choosing to perform the reconstruction, deciding when to perform it and which surgical technique to choose from among several available is quite complex, and may result in regret. The author provides a brief literature review on this subject and comments on the work carried out by Cai and Momeni, comparing data and results.Level of Evidence V This journal requires that authors assign a level of evidence to each article. For a full description of these evidence-based medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.

An unusual adverse event of botulinum toxin injection in the lower face.

BACKGROUND: In the lower third of the face, botulinum toxin A is used to obtain a more harmonious contour. The anatomical complexity of the face requires a more precise application of botulinum toxin. AIM: Describe the treatment of an unexpected effect after botulinum toxin A application in the lower third of the face. PATIENT/METHODS: A 51-year-old female patient presented to our clinic for application of botulin toxin aiming to improve facial wrinkles and contour. 50 U of botulinum toxin type A were applied to the upper third and 20 U to the lower third of the face. The patient returned after 10 days with a smile asymmetry and an excessive and unconscious lowering on the right side of the lower lip while laughing. So, 3 U of botulinum toxin were applied to the center of the depressor labii inferioris muscle on the right side. RESULTS: The patient returned with a satisfactory correction of the smile asymmetry. CONCLUSION: Although treatments with botulinum toxin A are considered safe, unexpected results can occur. In our case, it probably occurred due to medial injection or diffusion of botulinum toxin A into depressor anguli oris muscle on the left side that may lead to paralysis of the left depressor labii inferioris muscle and downward hyperfunction of the unaffected right lower lip. The correction was made with a new injection of botulinum toxin A in the depressor labii inferioris hyperactive muscle at the right side.

Escola e laço fraterno / Escuela y lazo fraterno / School and fraternal bond / L'école et le lien fraternel

Este artigo recupera algumas das características civilizatórias que marcaram o advento das sociedades da modernidade democrática e destaca aquele que seria o traço distintivo da escola republicana. A identificação desse traço permite confrontar as reivindicações de movimentos que atualmente afirmam a prioridade da educação familiar sobre a educação escolar e interrogar alguns dos pressupostos dos ideólogos de uma "educação para a sociabilidade democrática", que muitas vezes desconsideram que uma educação escolar comprometida com promoção da experiência democrática passa, antes de tudo, pela afirmação da escola como instituição implicada com a produção de uma filiação simbólica comum. É essa afirmação que nos permitirá vislumbrar, por fim, o limite da família no processo da transmissão simbólica que balizará o acontecimento de um sujeito interpelado na modernidade como indivíduo e como cidadão.

Este artículo busca recuperar algunas de las características civilizadoras que marcan el advenimiento de las sociedades de la modernidad democrática para, a partir de allí, rescatar el rasgo distintivo de la escuela republicana. La identificación de este rasgo que caracteriza a la escuela soñada por la modernidad democrática permite, en un segundo momento, confrontar el argumento en el que se basan los movimientos actuales que priorizan la educación familiar por sobre la escolar y, al mismo tiempo, cuestionar algunos de los presupuestos idealistas de una "educación para sociabilidad democrática" - que, muchas veces, desconocen que una educación escolar comprometida con la promoción de la experiencia democrática se basa, principalmente, en la consolidación de la escuela como institución implicada en la producción de una filiación simbólica común.

Starting with a brief consideration on the movements that presently claim the priority of family education over school education, and those that reclaim the possibility of an education fully ran in a home territory, this paper aims to recover some of the civilizatory characteristics that mark the advent of the societies of the democratic modernity to, from this point, retrieve what would be the distinctive trait of the Republican school. The identification of this trait that distinguishes the dreamed school in the advent of the democratic modernity allows us, at a second moment, to confront the argument that serves as a basis for these movements' claims. At the same time, it allows us to question some of the assumptions from the ideologues of an "education for the democratic sociability" - who, often, end for considering that a school education committed with the promotion of the democratic experience passes, first and foremost, through the affirmation of school as an institution implicated with the production of a common filiation.

Cet article vise à reprendre, en premier lieu, certains éléments civilisateurs caractérisant l'avènement des sociétés de la modernité démocratique afin d'en dégager celui qui serait le trait distinctif de l'école républicaine. D'une part, ce travail d'identification nous permet de confronter les propos des mouvements qui revendiquent, de nos jours, la priorité de l'éducation familiale par rapport à l'éducation scolaire. D'autre part, il est également nécessaire de remettre en cause quelques présupposés des théoriciens d'une « éducation pour la sociabilité démocratique ¼ d'autant plus qu'ils ne prennent pas assez souvent en compte certains aspects importants d'une éducation scolaire engagée à la promotion de l'expérience démocratique. Au terme de l'analyse, cette étude cherche à mettre en évidence que la scolarisation liée à la sociabilité démocratique passe, avant tout, par l'affirmation d'une école considérée comme une institution orientée envers la production d'une filiation commune.

Invited Discussion on: Management of Expander and Implant Associated Infections in Breast Reconstruction.

The author presents an objective review on "Management of Expander and Implant Associated Infections in Breast Reconstruction," discussing different points related to this subject, such as infection definition, identification of risk factors, related microorganisms, surgical techniques, preventive measures, antibiotic prophylaxis and therapy. Flaws in methodologies are identified and points of discrepancy in data and treatment results from previously published studies are pointed out, with discussion of possible causes for these inconsistencies. LEVEL OF EVIDENCE V: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.

Invited Discussion on: Breast Implant-Associated Anaplastic Large Cell Lymphoma (BIA-ALCL) and the Textured Implant Crisis.

The author discusses some important points about breast implant-associated anaplastic large cell lymphoma (BIA-ALCL) and adds new and updated information about the disease (ALCL). LEVEL OF EVIDENCE V: This journal requires that authors assign a level of evidence to each article. For a full description of these evidence-based medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.

Evaluation of mitogenome sequence concordance, heteroplasmy detection, and haplogrouping in a worldwide lineage study using the Precision ID mtDNA Whole Genome Panel.

The emergence of Massively Parallel Sequencing technologies enabled the analysis of full mitochondrial (mt)DNA sequences from forensically relevant samples that have, so far, only been typed in the control region or its hypervariable segments. In this study, we evaluated the performance of a commercially available multiplex-PCR-based assay, the Precision ID mtDNA Whole Genome Panel (Thermo Fisher Scientific), for the amplification and sequencing of the entire mitochondrial genome (mitogenome) from even degraded forensic specimens. For this purpose, more than 500 samples from 24 different populations were selected to cover the vast majority of established superhaplogroups. These are known to harbor different signature sequence motifs corresponding to their phylogenetic background that could have an effect on primer binding and, thus, could limit a broad application of this molecular genetic tool. The selected samples derived from various forensically relevant tissue sources and were DNA extracted using different methods. We evaluated sequence concordance and heteroplasmy detection and compared the findings to conventional Sanger sequencing as well as an orthogonal MPS platform. We discuss advantages and limitations of this approach with respect to forensic genetic workflow and analytical requirements.

Pinna synthetic mold for otoplasty techniques application / Molde sintético auricular para aplicação de técnicas de otoplastia

Abstract Introduction The ear deformity Tanzer type V, also known as prominent ears, is the most common genetic defect of the pinna. The surgery designed for its correction is known as otoplasty. This esthetic surgery can be performed using different techniques, which requires great skill of its operator. Objective The purpose of this work is the development of a new tool for otoplasty techniques training, aimed on the possibility to minimize errors during the otoplasty. Methods Synthetic molds of the external ear from patients with Tanzer type V deformity were made, using silicone material and rayon. Results The main procedures of otoplasty could be performed in the molds made of silicone and rayon with a good esthetic result. Conclusion The elaborated molds had identical size and shape of a human ear and could be positioned in the same shape of the patient ears. Thus, the synthetic molds were presented as promising simulation tools for the training and surgical enhancement of otoplasty, especially for doctors beginners.

Resumo Introdução A deformidade da orelha tipo V de Tanzer, também conhecida como orelhas proeminentes, é o defeito genético mais comum da aurícula. A cirurgia criada para sua correção é conhecida como otoplastia. Essa cirurgia estética pode ser feita com diferentes técnicas, o que requer grande habilidade de seu operador. Objetivo O objetivo desse trabalho é o desenvolvimento de uma nova ferramenta para o treinamento de técnicas de otoplastia, com o objetivo de minimizar erros durante a otoplastia. Método Foram feitos moldes sintéticos da orelha externa de pacientes com deformidade tipo V de Tanzer com material de silicone e rayon. Resultados Os principais procedimentos de otoplastia foram feitos nos moldes de silicone e rayon com um bom resultado estético. Conclusão Os moldes elaborados tinham tamanho e forma idênticos aos de uma orelha humana e puderam ser posicionados no mesmo formato das orelhas dos pacientes. Assim, os moldes sintéticos foram apresentados como ferramentas de simulação promissoras para o treinamento e aperfeiçoamento cirúrgico da otoplastia, especialmente para médicos iniciantes.

Pinna synthetic mold for otoplasty techniques application.

INTRODUCTION: The ear deformity Tanzer type V, also known as prominent ears, is the most common genetic defect of the pinna. The surgery designed for its correction is known as otoplasty. This esthetic surgery can be performed using different techniques, which requires great skill of its operator. OBJECTIVE: The purpose of this work is the development of a new tool for otoplasty techniques training, aimed on the possibility to minimize errors during the otoplasty. METHODS: Synthetic molds of the external ear from patients with Tanzer type V deformity were made, using silicone material and rayon. RESULTS: The main procedures of otoplasty could be performed in the molds made of silicone and rayon with a good esthetic result. CONCLUSION: The elaborated molds had identical size and shape of a human ear and could be positioned in the same shape of the patient ears. Thus, the synthetic molds were presented as promising simulation tools for the training and surgical enhancement of otoplasty, especially for doctors beginners.

Avaliação do ensino e aprendizagem de noções básicas de tratamento de queimados entre alunos do curso de medicina / Assessment of teaching and learning burn treatment basics among medical students

Introdução: Agravo de grande importância biopsicossocial, queimadura é um tema crucial na formação médica, uma vez que recém-formados se deparam com pacientes que sofreram queimaduras nos mais variados cenários profissionais. Métodos: Este estudo avaliou o ensino e a aprendizagem do tema queimaduras em alunos do curso de Medicina de uma faculdade pública do Estado de São Paulo; foi aplicado um questionário específico para avaliar o conhecimento sobre o atendimento inicial ao paciente queimado na primeira série (grupo 1 - 54 estudantes), e na sexta série (grupo 2 - 60 estudantes). Resultados: Observou-se, no grupo 1, grande desconhecimento sobre o tema, com média geral de desconhecimento ("erros") de 70,98% e de acertos de 29,02%; no grupo 2, essas taxas foram de 36,66 % e 63,34 %, respectivamente. Somente 63,34% dos alunos do sexto ano conseguiriam conduzir adequadamente o tratamento de um paciente queimado e, consequentemente, oferecer a este paciente um melhor prognóstico. Conclusões: Os dados são preocupantes, uma vez que não se verificou conhecimento nem preparo adequados dos formandos para atendimento de pacientes queimados, e que queimaduras são situações de emergência que exigem intervenção médica rápida, adequada e eficiente, a fim de reduzir a morbimortalidade das vítimas.

Introduction: Burns, an injury of high biopsychosocial importance, are a crucial issue in medical training, and newly graduated students face burn patients in a variety of professional settings. Methods: This study assessed the teaching and learning of burns among medical students at a public facility in the State of São Paulo; a specific questionnaire was applied to evaluate first year (Group 1: 54 students) and sixth year (Group 2: 60 students) medical students' knowledge about the initial care of burn patients. Results: In Group 1, there was a substantial lack of knowledge about the subject, with a general average incorrect answer rate ("errors") of 70.98% and a correct answer rate of 29.02%; in Group 2, these rates were 36.66% and 63.34%, respectively. Only 63.34% of the sixth-year students would be able to adequately conduct the treatment of a burn patient, gauged by their ability to offer a patient a better prognosis. Conclusions: The data are disturbing, since adequate knowledge and preparation of the trainees for the care of burn patients has not been verified, and burns are emergency situations that require prompt, adequate, and efficient medical intervention to reduce patients' morbidity and mortality.

Lack of gene-language correlation due to reciprocal female but directional male admixture in Austronesians and non-Austronesians of East Timor.

Nusa Tenggara, including East Timor, located at the crossroad between Island Southeast Asia, Near Oceania, and Australia, are characterized by a complex cultural structure harbouring speakers from two different major linguistic groups of different geographic origins (Austronesian (AN) and non-Austronesian (NAN)). This provides suitable possibilities to study gene-language relationship; however, previous studies from other parts of Nusa Tenggara reported conflicting evidence about gene-language correlation in this region. Aiming to investigate gene-language relationships including sex-mediated aspects in East Timor, we analysed the paternally inherited non-recombining part of the Y chromosome (NRY) and the maternally inherited mitochondrial (mt) DNA in a representative collection of AN- and NAN-speaking groups. Y-SNP (single-nucleotide polymorphism) data were newly generated for 273 samples and combined with previously established Y-STR (short tandem repeat) data of the same samples, and with previously established mtDNA data of 290 different samples with, however, very similar representation of geographic and linguistic coverage of the country. We found NRY and mtDNA haplogroups of previously described putative East/Southeast Asian (E/SEA) and Near Oceanian (NO) origins in both AN and NAN speakers of East Timor, albeit in different proportions, suggesting reciprocal genetic admixture between both linguistic groups for females, but directional admixture for males. Our data underline the dual genetic origin of East Timorese in E/SEA and NO, and highlight that substantial genetic admixture between the two major linguistic groups had occurred, more so via women than men. Our study therefore provides another example where languages and genes do not conform due to sex-biased genetic admixture across major linguistic groups.

Pacifiplex: an ancestry-informative SNP panel centred on Australia and the Pacific region.

The analysis of human population variation is an area of considerable interest in the forensic, medical genetics and anthropological fields. Several forensic single nucleotide polymorphism (SNP) assays provide ancestry-informative genotypes in sensitive tests designed to work with limited DNA samples, including a 34-SNP multiplex differentiating African, European and East Asian ancestries. Although assays capable of differentiating Oceanian ancestry at a global scale have become available, this study describes markers compiled specifically for differentiation of Oceanian populations. A sensitive multiplex assay, termed Pacifiplex, was developed and optimized in a small-scale test applicable to forensic analyses. The Pacifiplex assay comprises 29 ancestry-informative marker SNPs (AIM-SNPs) selected to complement the 34-plex test, that in a combined set distinguish Africans, Europeans, East Asians and Oceanians. Nine Pacific region study populations were genotyped with both SNP assays, then compared to four reference population groups from the HGDP-CEPH human diversity panel. STRUCTURE analyses estimated population cluster membership proportions that aligned with the patterns of variation suggested for each study population's currently inferred demographic histories. Aboriginal Taiwanese and Philippine samples indicated high East Asian ancestry components, Papua New Guinean and Aboriginal Australians samples were predominantly Oceanian, while other populations displayed cluster patterns explained by the distribution of divergence amongst Melanesians, Polynesians and Micronesians. Genotype data from Pacifiplex and 34-plex tests is particularly well suited to analysis of Australian Aboriginal populations and when combined with Y and mitochondrial DNA variation will provide a powerful set of markers for ancestry inference applied to modern Australian demographic profiles. On a broader geographic scale, Pacifiplex adds highly informative data for inferring the ancestry of individuals from Oceanian populations. The sensitivity of Pacifiplex enabled successful genotyping of population samples from 50-year-old serum samples obtained from several Oceanian regions that would otherwise be unlikely to produce useful population data. This indicates tests primarily developed for forensic ancestry analysis also provide an important contribution to studies of populations where useful samples are in limited supply.

Variação espacial de cátions, ânions e variáveis físico-químicas no rio Solimões-Amazonas entre Manaus e Jutaí, bacia amazônica / Spatial variation of cations, anions and physicochemical variables in the Solimões-Amazonas River, between Manaus and Jutaí, Amazon Basin

Cátions e ânions majoritários são espécies químicas de grande importância nos diversos compartimentos aquáticos, porém, pouco abordados de forma exclusiva na bacia amazônica. Neste sentido, o objetivo deste estudo foi avaliar a dinâmica espacial de Na+, Ca2+, K+, Mg2+, Cl- e SO4 2- em um trecho do rio Solimões-Amazonas, levando-se em consideração a alcalinidade, potencial hidrogeniônico (pH), condutividade elétrica (CE), sólidos totais em suspensão (STS) e carbono orgânico total (COT). As coletas foram realizadas em maio, julho e setembro de 2012 em sete pontos na calha principal do rio Solimões-Amazonas e na foz de alguns dos principais tributários: rios Jutaí, Juruá, Japurá e Purus. Os cátions e ânions foram analisados por cromatografia de íons, STS por gravimetria, pH por potenciometria, alcalinidade por titulação potenciométrica, CE por condutimetria e o COT por oxidação catalítica por combustão. As concentrações médias dos cátions seguiram esta ordem Ca2+>Na+>Mg2+>K+ e dos ânions SO4 2->Cl-. De montante à jusante a tendência foi diminuição nas concentrações de cátions e ânions, das variáveis pH, CE, STS e alcalinidade, além do aumento das concentrações de COT. O presente estudo evidenciou mudanças nas características químicas sofridas pelo rio Solimões em seu curso. O aporte dos tributários pode ser apontado como um dos fatores responsáveis por essas modificações.

Major cations and anions are chemical species with great importance in different water compartments. However, no specific attention has been given to them in the Amazon basin. This study aimed to assess the spatial dynamic of Na+, Ca2+, K+, Mg2+, Cl- and SO4 2- in a specific section of the Solimões-Amazonas river taking into account the alkalinity, hydrogen potential (pH), conductivity electric (EC), total suspended solids (TSS) and total organic carbon (TOC). Samples were collected in May, July and September 2012 in seven points in the Solimões-Amazonas River and in the mouth of some of its main tributaries: rivers Jutaí, Juruá, Japurá and Purus. Cations and anions were analyzed by ion chromatography, TSS by gravimetry, pH by potentiometry, alkalinity by potentiometric titration, EC by conductimetry and TOC by catalytic oxidation combustion. The average cation concentrations followed this order: Ca2+ > Na+ > Mg2+ > K+ and anion SO4 2- > Cl. From the upstream of the river towards its downstream section there was a tendency for a decrease in cations and anions accumulation, pH, EC, TSS and alkalinity. On the other hand, there was an increase in TOC. The present study showed changes in chemical characteristics of Solimões River along its course. It was observed the contribution of the tributary rivers as one of the responsible factors for these changes.

Human settlement history between Sunda and Sahul: a focus on East Timor (Timor-Leste) and the Pleistocenic mtDNA diversity.

BACKGROUND: Distinct, partly competing, "waves" have been proposed to explain human migration in(to) today's Island Southeast Asia and Australia based on genetic (and other) evidence. The paucity of high quality and high resolution data has impeded insights so far. In this study, one of the first in a forensic environment, we used the Ion Torrent Personal Genome Machine (PGM) for generating complete mitogenome sequences via stand-alone massively parallel sequencing and describe a standard data validation practice. RESULTS: In this first representative investigation on the mitochondrial DNA (mtDNA) variation of East Timor (Timor-Leste) population including >300 individuals, we put special emphasis on the reconstruction of the initial settlement, in particular on the previously poorly resolved haplogroup P1, an indigenous lineage of the Southwest Pacific region. Our results suggest a colonization of southern Sahul (Australia) >37 kya, limited subsequent exchange, and a parallel incubation of initial settlers in northern Sahul (New Guinea) followed by westward migrations <28 kya. CONCLUSIONS: The temporal proximity and possible coincidence of these latter dispersals, which encompassed autochthonous haplogroups, with the postulated "later" events of (South) East Asian origin pinpoints a highly dynamic migratory phase.

Genetic variation of 12 X-chromosomal STR loci in an East Timor sample.

East Timor (República Democrática de Timor Leste) is a country with a population around 1 million inhabitants located in Southeast Asia and composed of 13 districts, including the eastern half of the Timor Island, Ataúro and Jaco islands, and the coastal enclave of Oecusse-Ambeno located in West Timor. Examples of the importance of X-chromosomal short tandem repeats (STRs) analysis include parentage identification, namely in cases involving father/daughter relationships, paternity in close relative deficiency cases without access to the putative father, maternity testing, and in rape or incest cases. In this study, 149 saliva samples were collected from unrelated individuals from East Timor and 12 X-chromosomal STRs genotyped using Investigator® Argus X-12 kit (Qiagen). A total of 13 alleles not included in Investigator Argus X-12 allelic ladder (off-ladder alleles) were found, four of which never reported (alleles 34.1 and 38.1 at DXS10134, allele 17.2 at DXS10074, and allele 28.1 at DXS10146). Allele 27.3 at DXS10101 and alleles 26, 28, and 29 at DXS10148 have already been observed in other populations but their frequencies are considerably higher in East Timor population. Allele frequencies and population statistic parameters were calculated for East Timor population and data contextualized in Southeast Asia/Pacific Region.

Low-fidelity bench models for basic surgical skills training during undergraduate medical education.

It is remarkable the reduction in the number of medical students choosing general surgery as a career. In this context, new possibilities in the field of surgical education should be developed to combat this lack of interest. In this study, a program of surgical training based on learning with models of low-fidelity bench is designed as a complementary alternative to the various methodologies in the teaching of basic surgical skills during medical education, and to develop personal interests in career choice.

Low-fidelity bench models for basic surgical skills training during undergraduate medical education / Modelos de bancada de baixa fidelidade para o treinamento de habilidades cirúrgicas básicas durante a graduação médica

It is remarkable the reduction in the number of medical students choosing general surgery as a career. In this context, new possibilities in the field of surgical education should be developed to combat this lack of interest. In this study, a program of surgical training based on learning with models of low-fidelity bench is designed as a complementary alternative to the various methodologies in the teaching of basic surgical skills during medical education, and to develop personal interests in career choice.

É notável a redução no número de estudantes de Medicina que escolhem a Cirurgia Geral como carreira. Neste contexto, novas possibilidades no campo do ensino cirúrgico devem ser desenvolvidas para combater este desinteresse. No presente estudo, um programa de treinamento cirúrgico baseado na aprendizagem em modelos de bancada de baixa fidelidade é delineado como uma alternativa complementar as diversas metodologias aplicadas no ensino das habilidades cirúrgicas básicas durante a graduação médica, bem como para desenvolver interesses pessoais na escolha da carreira.