Toxicity of glyphosate and aminomethylphosphonic acid (AMPA) to the early stages of development of Steindachneridion melanodermatum, an endangered endemic species of Southern Brazil.

This study aimed to evaluate glyphosate (GLY) and aminomethylphosphonic acid (AMPA) toxicity at 65, 650, and 6500 µg L-1 to the initial stages of development of Steindachneridion melanodermatum, an endangered endemic species from the Iguaçu River, assessing hatching, survival, total larval length, deformities, oxidative stress biochemical biomarkers, and neurotoxicity. Overall, looking at the sum of responses through the integrated biomarker response, the species was more sensitive to AMPA than GLY, especially at the lower concentration of 65 µg L-1, which induced mortality, deformities, underdevelopment, and oxidative stress. Considering the risk of exposure and the importance of conservation of the highly endemic ichthyofauna of this basin, it is urgent to investigate and regulate both GLY and AMPA levels at the Iguaçu River to protect not only this species, but the entire ecosystem.

Prepubertal oral exposure to relevant doses of acrylamide impairs the testicular antioxidant system in adulthood, increasing protein carbonylation and lipid peroxidation.

The increased prevalence of human infertility due to male reproductive disorders has been linked to extensive exposure to chemical endocrine disruptors. Acrylamide (AA) is a compound formed spontaneously during the thermal processing of some foods that are mainly consumed by children and adolescents. We previously found that prepubertal exposure to AA causes reduced sperm production and functionality. Oxidative stress is recognized as the main cause of reduced sperm quality and quantity. In this sense, our objective was to evaluate the expression and activity of genes related to enzymatic antioxidant defense, nonprotein thiols, lipid peroxidation (LPO), protein carbonylation (PC) and DNA damage in the testes of rats exposed to acrylamide (2.5 or 5 mg/kg) from weaning to adult life by gavage. For the AA2.5 and AA5 groups, there were no alterations in the transcript expression of genes related to enzymatic antioxidant defense. The enzymatic activities and metabolic parameters were also not affected in the AA2.5 group. For the AA5 group, the enzymatic activities of G6PDH and GPX were reduced, SOD was increased, and protein carbonylation (PC) was increased. Data were also evaluated by Integrate Biomarker Response (IBRv2), a method to analyze and summarize the effects on biomarkers between doses. The IBRv2 index was calculated as 8.9 and 18.71 for AA2.5 and AA5, respectively. The following biomarkers were affected by AA2.5: decreased enzymatic activities of G6PDH, SOD, and GPX, increased GST and GSH, increased LPO and PC, and decreased DNA damage. For AA5, decreased enzymatic activities of G6PDH, GST, CAT and GPX, increased SOD and GSH, increased PC, and decreased LPO and DNA damage were observed. In conclusion, AA exposure during the prepubertal period causes imbalances in the testicular enzymatic antioxidant defense, contributing to the altered spermatic scenario in the testes of these rats.

Organic and inorganic pollutants in Jordão and Iguaçu rivers southern Brazil impact early phases of Rhamdia quelen and represent a risk for population.

The Iguaçu River basin presents high ecological importance due to its expressive endemic ichthyofauna rate, but chemical pollution may threat this biodiversity. Jordão River is one of the main tributaries of Iguaçu River and contribute to this pollution status, since it drains large agricultural areas receiving domestic and industrial effluents before flowing into the Iguaçu River. The objective of the current study was to evaluate the toxic effects of the Iguaçu, Jordão, and the combination of their waters to the embryo-larval phase of R. quelen, investigating the consequences to the population by means of mathematical modelling. R. quelen fertilized eggs were exposed for 96 h to water samples from Iguaçu River upstream (IR), Jordão River (JR), and downstream of both rivers (MR). The analysis of micropollutants in the water showed that JR presented the most complex mixture of substances and elements, followed by IR, while MR showed the lower number of micropollutants detected. Survival rate was not a sensitive endpoint, while the deformity indices were higher in individuals exposed to water from the three studied sites. Superoxide dismutase activity was increased in MR, while non-protein thiol levels were reduced in MR and JR showing the antioxidant mechanism activation. The mathematical modelling revealed that fish exposed to JR would lead to the greater population reduction (46.19%), followed by IR (40.48%) and MR (33.33%). Although the results showed toxicity in all studied sites, the JR site is the most impacted by micropollutants but decrease its toxicity after dilution with Iguaçu River.