RESUMEN
The cat flea, Ctenocephalides felis (Siphonaptera: Pulicidae) (Bouché), is the most common flea species found on cats and dogs worldwide. We investigated the genetic identity of the cosmopolitan subspecies C. felis felis and evaluated diversity of cat fleas from Australia, Fiji, Thailand and Seychelles using mtDNA sequences from cytochrome c oxidase subunit I (cox1) and II (cox2) genes. Both cox1 and cox2 confirmed the high phylogenetic diversity and paraphyletic origin of C. felis felis. The African subspecies C. felis strongylus (Jordan) is nested within the paraphyletic C. felis felis. The south East Asian subspecies C. felis orientis (Jordan) is monophyletic and is supported by morphology. We confirm that Australian cat fleas belong to C. felis felis and show that in Australia they form two distinct phylogenetic clades, one common with fleas from Fiji. Using a barcoding approach, we recognize two putative species within C. felis (C. felis and C. orientis). Nucleotide diversity was higher in cox1 but COX2 outperformed COX1 in amino acid diversity. COX2 amino acid sequences resolve all phylogenetic clades and provide an additional phylogenetic signal. Both cox1 and cox2 resolved identical phylogeny and are suitable for population structure studies of Ctenocephalides species.
Asunto(s)
Ctenocephalides/genética , Variación Genética , Proteínas de Insectos/genética , Proteínas Mitocondriales/genética , Filogenia , Animales , Australia , Complejo IV de Transporte de Electrones/genética , Marcadores Genéticos/genética , Datos de Secuencia Molecular , Análisis de Secuencia de ADN/veterinariaRESUMEN
Toxoplasma gondii-like organisms were seen in the heart, kidney, liver, lung, lymph node, spleen, small intestine, and stomach from 2 koalas that died suddenly in a fauna park in Sydney, Australia. Diagnosis was confirmed by immunohistochemical staining. This is the first confirmed report of toxoplasmosis in an arboreal marsupial.