The impact of six-week dihydrogen-pyrroloquinoline quinone supplementation on mitochondrial biomarkers, brain metabolism, and cognition in elderly individuals with mild cognitive impairment: a randomized controlled trial.

OBJECTIVES: To assess the impact of medium-term supplementation with dihydrogen and pyrroloquinoline quinone (PQQ) on mitochondrial biomarkers, brain metabolism, and cognition in elderly individuals diagnosed with mild cognitive impairment. DESIGN: A parallel-group, randomized, placebo-controlled, double-blind experimental design, maintaining a 1:1 allocation ratio between the experimental group (receiving the dihydrogen-producing minerals and PQQ) and the control group (receiving the placebo) throughout the trial. SETTING AND PARTICIPANTS: Thirty-four elderly individuals with mild cognitive impairment (mean age 71.9 ± 3.8 years; 28 females) voluntarily provided written consent to participate in this trial. Participants were assigned in a double-blind parallel-group design to receive either a dihydrogen-PQQ mixture (Alpha Hope®, CalerieLife, Irvine, CA) or placebo twice daily for a 6-week intervention period. METHODS: The primary endpoint was the change in serum brain-derived neurotrophic factor (BDNF) from baseline to the 6-week follow-up; secondary outcomes included cognitive function indices, specific metabolites in brain tissue, brain oxygenation, and the prevalence and severity of side effects. Interaction effects (time vs. intervention) were evaluated using two-way ANOVA with repeated measures and Friedman's 2-way ANOVA by ranks, for normally distributed data with homogeneous variances and non-homogeneous variances, respectively. RESULTS: Dihydrogen-PQQ resulted in a significant elevation in serum BDNF levels at the six-week follow-up (P = 0.01); conversely, no changes in BDNF levels were observed in the placebo group throughout the study duration (P = 0.27). A non-significant trend in the impact of interventions on BDNF levels was observed (treatment vs. time interaction, P = 0.14), suggesting a tendency for dihydrogen-PQQ to upregulate BDNF levels compared to the placebo. A significant interaction effect was observed for the Alzheimer's Disease Assessment Scale-Cognitive subscale (ADAS-Cog) scores in the orientation domain (P = 0.03), indicating the superiority of dihydrogen-PQQ over placebo in enhancing this cognitive aspect. Cerebral oxygenation saturation exhibited a significant increase following the administration of the dihydrogen-PQQ mixture, from 48.4 ± 7.2% at baseline to 52.8 ± 6.6% at 6-week post-administration (P = 0.005). In addition, brain N-acetyl aspartate levels significantly increased at seven out of thirteen locations post-intervention in participants receiving the mixture (P ≤ 0.05). CONCLUSIONS: Despite the limited number of participants included in the study for interpreting clinical parameters, the dihydrogen-PQQ mixture blend shows promise as a potential dietary intervention for enhancing mental orientation and brain metabolism in individuals with age-related mild cognitive decline.

Optimizing strength training protocols in young females: A comparative study of velocity-based and percentage-based training programs.

The purpose of this study was to compare the effects of velocity-based strength training (VBT) and percentage-based strength training (PBT) on absolute strength, explosive strength, speed, and agility, as well as markers of muscle damage after 6 weeks of exercise programs. The study included 30 young female individuals, divided into three groups of 10 participants: VBT, PBT, and control group. The main findings indicated that the VBT group and PBT group showed significant improvement in 1RM squat exercise (Δ% 27.87 and Δ% 8.98, respectively) and 1RM bench press (Δ% 14.47 and Δ% 8.65, respectively), but a greater enhancement was observed in the VBT group. In addition, VBT induced substantial changes in SJ (Δ% 14.32) and CMJ height (Δ% 7.69), while PBT had an improvement only in the SJ test (Δ% 6.72). The improvement noted in the VBT group could be attributed to its ability to tailor training intensity according to the speed of movement execution. This approach allows athletes to perform each repetition as fast as possible, thus maintaining an optimal intensity for explosive strength development. The capacity of VBT to adapt training intensity based on the speed of movement execution may be the key factor contributing to these results. Therefore, coaches and athletes should consider implementing VBT as a valuable tool to optimize strength and power development. In conclusion, VBT induced greater improvement in the 1RM squat, 1RM bench press, SJ, and CMJ compared to the group that performed the traditional strength training modality. Therefore, VBT is considered a more effective training tool regarding the development of absolute and explosive strength in young women.

Eight-Week Creatine-Glucose Supplementation Alleviates Clinical Features of Long COVID.

Preliminary studies demonstrated beneficial effects of dietary creatine across different post-viral fatigue syndromes. Creatine is often co-administered with glucose to improve its potency yet whether glucose boost the efficacy of creatine in long COVID remains currently unknown. In this report, we investigate the effects of 8-wk creatine intake with and without glucose on patient-reported outcomes, exercise tolerance, and tissue creatine levels in patients with long COVID. Fifteen male and female long COVID adult patients (age 39.7±16.0 y; 9 women) with moderate fatigue and at least one of additional long COVID-related symptoms volunteered to participate in this randomized controlled parallel-group interventional trial. All patients were allocated in a double-blind parallel-group design (1 : 1 : 1) to receive creatine (8 g of creatine monohydrate per day), a mixture of creatine and glucose (8 g of creatine monohydrate and 3 g of glucose per day), or placebo (3 g of glucose per day) t.i.d. during an 8-wk intervention interval. Two-way ANOVA with repeated measures (treatment vs. time interaction) revealed significant differences in changes in total creatine levels between the groups, showing an interaction effect at two brain locations (right precentral white matter F=34.740, p=0.008; partial η2=0.72; left paracentral grey matter F=19.243, p=0.019; partial η2=0.88), with creatine and creatine-glucose outcompeted placebo to elevate creatine levels at these two locations. Several long COVID symptoms (including body aches, breathing problems, difficulties concentrating, headache, and general malaise) were significantly reduced in creatine-glucose group at 8-wk follow-up (p≤0.05); the effect sizes for reducing body aches, difficulties concentrating, and headache were 1.33, 0.80, and 1.12, respectively, suggesting a large effect of creatine-glucose mixture for these outcomes. Our preliminary findings suggest that supplying exogenous creatine with glucose could be recommended as an effective procedure in replenishing brain creatine pool and alleviating long COVID features in this prevalent condition.

Effects of an integrative warm-up method on the range of motion, core stability, and quality of squat performance of young adults.

Introduction: This research aims to determine the effects of an integrative warm-up method on the range of motion in joints of the lower extremities, the strength of the stabilizer trunk muscles, and the quality of the basic movement patterns in older adolescents. Methods: The study sample consisted of 88 male students (age 20.1 ± 0.5). They were randomly divided into four groups: one control group (CG) (n = 17; 180.8 ± 7.9 cm; 82.3 ± 8.3 kg) and three experimental groups (EG): EG1 (n = 23; 180.9 ± 7.0 cm; 78.5 ± 9.5 kg), EG2 (n = 31; 182.2 cm ± 7.3 cm; 79.5 ± 11.5 kg), and EG3 (n = 17; 183.3 ± 4.9 cm; 77.5 ± 11.8 kg). The participants were subjected to a 6-week experimental treatment: EG1 once, EG2 twice, and EG3 three times a week. The experimental treatment consisted of four sub-phases representing the integrative warm-up Method: 1) Inhibition (self-myofascial release using a foam roller); 2) Lengthening (Static stretching in a maximum range of motion position); 3) Activation (Positional isometrics muscle activation of the trunk and gluteus); 4) Integration (Integrated all the previous phases into one complex movement pattern). Based on the covariance analysis (ANCOVA), statistically significant treatment effects were observed and positive changes were determined in all experimental groups. Results: The differences between groups were observed in the following variables: Overhead Squat Assessment (p = 0.000; ηp2=0.318), range of motion of left hip flexion (p = 0.000; ηp2=0.371), range of motion of right hip flexion (p = 0.000; ηp2=0.051) and range of motion of right hip extension (p = 0.051; ηp2=0.088), Double Leg Lowering Test (F = 2.411; p = 0.014; ηp2=0.014) and range of combined motion (plantar and dorsiflexion) of left ankle joint (p = 0.000; ηp2=0.299). There was no significant difference in the Plank Test (F = 1.007; p = 1.007; ηp2=0.035), range of combined motion (plantar and dorsiflexion) of right ankle joint (p = 0.088; ηp2=0.170) and range of motion of left hip extension (p = 0.158; ηp2=0.060). The participants of CG statistically significantly differed from EG1, EG2, and EG3 in the squat performance after the applied treatment. Discussion: The effect of the treatment was the occurrence of a transformational processes in almost all measured variables. It can be concluded that the integrative method is effective and applicable in practice for both young adults and recreational athletes.

Effects of six-month creatine supplementation on patient- and clinician-reported outcomes, and tissue creatine levels in patients with post-COVID-19 fatigue syndrome.

Dietary creatine has been recently put forward as a possible intervention strategy to reduce post-COVID-19 fatigue syndrome yet no clinical study so far evaluated its efficacy and safety for this perplexing condition. In this parallel-group, randomized placebo-controlled double-blind trial, we analyzed the effects of 6-month creatine supplementation (4 g of creatine monohydrate per day) on various patient- and clinician-reported outcomes, and tissue creatine levels in 12 patients with post-COVID-19 fatigue syndrome. Creatine intake induced a significant increase in tissue creatine levels in vastus medialis muscle and right parietal white matter compared to the baseline values at both 3-month and 6-month follow-ups (p < .05). Two-way analysis of variance with repeated measures revealed a significant difference (treatment vs. time interaction) between interventions in tissue creatine levels (p < .05), with the creatine group was superior to placebo to augment creatine levels at vastus medialis muscle, left frontal white matter, and right parietal white matter. Creatine supplementation induced a significant reduction in general fatigue after 3 months of intake compared to baseline values (p = .04), and significantly improved scores for several post-COVID-19 fatigue syndrome-related symptoms (e.g., ageusia, breathing difficulties, body aches, headache, and difficulties concentrating) at 6-month follow-up (p < .05). Taking creatine for 6 months appears to improve tissue bioenergetics and attenuate clinical features of post-COVID-19 fatigue syndrome; additional studies are warranted to confirm our findings in various post-COVID-19 cohorts.

What Determines the Competitive Success of Young Croatian Wrestlers: Anthropometric Indices, Generic or Specific Fitness Performance?

Identifying factors influencing wrestling performance is important for determining which capacities should be developed the most. This research aimed to investigate whether anthropometric indices, generic fitness, and specific fitness performance determine the competitive success of young wrestlers. This research included 49 Croatian Greco-Roman male wrestlers aged 17.75 ± 1.51 years. Variables included training and competing experience, anthropometric indices, generic fitness (countermovement jump and handgrip strength), and specific wrestling fitness test (SWFT). Wrestlers were separated into medallists and non-medallists (i.e., wrestlers who won a medal at the previous National Championship and wrestlers who did not win a medal, respectively). The t-test for independent samples was used to determine the differences between the two categories in all variables. Moreover, discriminant analysis was performed to identify differences in a multivariate manner. Medallists and non-medallists did not differ in anthropometric indices and wrestling experience. Medallists had better results in the countermovement jump (t = 2.55, p < 0.01), handgrip strength (t = 2.77, p < 0.01), and SWFT performance (t = 2.29, p < 0.05) than non-medallists. The discriminant analysis confirmed that performance categories differed in generic and specific fitness tests (Wilks' Lambda = 0.73, p < 0.05). It could be suggested that coaches should develop both the generic and specific fitness of their wrestlers to become more successful at competitions.

The Effects of Hydrogen-Rich Water on Blood Lipid Profiles in Clinical Populations: A Systematic Review and Meta-Analysis.

Over the last two decades, a plethora of disease models and human studies have confirmed the beneficial effects of molecular hydrogen (H2), a simple biotherapeutic gas. Recent small-scale studies evaluating the effects of hydrogen-rich water (HRW) on various metabolic conditions pointed to advantageous effects of HRW in regulating blood lipid profiles. However, to the best of the authors' knowledge, no systematic review and/or meta-analysis (SRMA) were published considering HRW consumption and lipid/lipoprotein status. Therefore, the aim of this SRMA was to assess the effects of HRW consumption on blood lipid panel in clinical populations. The search strategy was designed using PRISMA guidelines, and the databases PubMed/Medline, Web of Science, and Scopus were explored from inception until 4 October 2022. A total of seven studies satisfied all the eligibility criteria and were included in SRMA. The results for the pooled meta-analysis showed a significant reduction in total cholesterol, low-density lipoprotein, and triglycerides after HRW intake (p = 0.01), with small to moderate effects (pooled SMD = -0.23 (from -0.40 to 0.05); pooled SMD = -0.22 (from -0.39 to 0.04); pooled SMD = -0.38 (from -0.59 to 0.18), respectively). Our findings indicate that drinking HRW can significantly improve lipid status in the clinical populations. Additional studies are warranted to further validate this connection.

Effects of Creatine Monohydrate on Endurance Performance in a Trained Population: A Systematic Review and Meta-analysis.

BACKGROUND: There is robust evidence that creatine monohydrate supplementation can enhance short-term high-intensity exercise in athletes. However, the effect of creatine monohydrate supplementation on aerobic performance and its role during aerobic activities is still controversial. OBJECTIVE: The purpose of this systematic review and meta-analysis was to evaluate the supplementation effects of creatine monohydrate on endurance performance in a trained population. METHODS: The search strategy in this systematic review and meta-analysis was designed following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, and PubMed/MEDLINE, Web of Science, and Scopus databases were explored from inception until 19 May, 2022. Only human experimental trials, controlled with a placebo group, evaluating the effects of creatine monohydrate supplementation on endurance performance in a trained population were analyzed in this systematic review and meta-analysis. The methodological quality of included studies was evaluated using the Physiotherapy Evidence Database (PEDro) scale. RESULTS: A total of 13 studies satisfied all the eligibility criteria and were included in this systematic review and meta-analysis. The results for the pooled meta-analysis showed a non-significant change in endurance performance after creatine monohydrate supplementation in a trained population (p = 0.47), with a trivial negative effect (pooled standardized mean difference = - 0.07 [95% confidence interval - 0.32 to 0.18]; I2 = 34.75%). Further, after excluding the studies not evenly distributed around the base of the funnel plot, the results were similar (pooled standardized mean difference = - 0.07 [95% confidence interval - 0.27 to 0.13]; I2 = 0%; p = 0.49). CONCLUSIONS: Creatine monohydrate supplementation was shown to be ineffective on endurance performance in a trained population. CLINICAL TRIAL REGISTRATION: The study protocol was registered in the Prospective Register of Systematic Review (PROSPERO) with the following registration number: CRD42022327368.

Motivation Profile of Youth Greco-Roman Wrestlers; Differences According to Performance Quality.

Athletes have to possess high motivation levels to perform each training session and competition at the highest level. Thus, the motivation of the wrestler is essential to reach the highest performance quality. The research included 47 Greco-Roman wrestlers aged 17.71 ± 1.62 years. Variables included anthropometric indices, sports motivation assessed by the revised Sport Motivation Scale (SMS-II), and competitive success (medal winners and non-winners at the National Championship). The Cronbach's alpha coefficients checked the internal consistency of the SMS-II. Differences between performance quality were determined by Cohen's d effect sizes, and MANOVA for motivation and anthropometric variables/body build variables. In the total sample, wrestlers had high levels of intrinsic motivation (5.97 ± 0.90), integrated (5.99 ± 0.83), and identified (6.08 ± 0.82) regulation, while they had low amotivation (2.53 ± 0.98) and external regulation (3.26 ± 1.24). Successful wrestlers had significantly higher intrinsic motivation than less successful wrestlers (Cohen's d = 0.76, moderate effect size). Results evidenced that wrestlers have high self-determined motivation, which is vital for maximal performance and persisting in sports. Future research should investigate wrestlers from other age groups to ultimately determine the sport motivation profile of wrestlers and enable their optimal sports development.

Creatine consumption and liver disease manifestations in individuals aged 12 years and over.

Despite the overwhelming safety evidence concerning creatine intake in various settings, there is still incomplete information whether dietary creatine affects liver health at the population level. The main aim of this cross-sectional population-based study was to evaluate the association between creatine intake through regular diet and liver disease manifestations, including liver fibrosis and hepatic steatosis, among individuals aged 12 years and over, using open-source data from the 2017-2018 U.S. National Health and Nutrition Examination Survey (NHANES). A total of 9254 male and female participants of all ages were included in the 2017-2018 NHANES round. We extracted data from the total sample population for participants who provided dietary data for individual foods via dietary interviews and examination data from liver ultrasound transient elastography. The final study sample consisted of 5957 participants (mean age 44.7 ± 21.0 years; 50.1% women), and the mean dietary creatine intake across the study population was 0.88 ± 0.71 g/day. Liver fibrosis and cirrhosis were diagnosed in 1703 (28.7%) and 288 (4.8%) participants, respectively; hepatic steatosis was identified in 2595 (43.7%) individuals. Binary logistic regression with multivariable model adjusted for age, gender, family income to poverty ratio, body mass index, total energy intake, and alcohol consumption showed that consuming more creatine (≥2 g/day) did not significantly increase the risk of liver fibrosis (OR = 0.92, 95% CI 0.70-1.21, p = .57), cirrhosis (OR = 0.94, 95% CI 0.53-1.65, p = .82), or hepatic steatosis (OR = 0.77, 95% CI 0.59-1.02, p = .07), as compared to participants who ingested <1 g of creatine daily. Dietary exposure to creatine through a regular diet is not associated with an increase in disease manifestations in individuals 12 years and over; further research is warranted to address the effects of excessive creatine intake (≥5 g/day) through a regular diet on liver health at the population level.

Prevalence of rapid weight loss in Olympic style wrestlers.

Background: The methodology applied for rapid weight loss (RWL) among elite wrestlers is quite unexplored. Therefore, the aim of this study was to analyze the prevalence of sources of influence and methods used for RWL and to determine the differences between wrestling styles. Methods: A total of 229 wrestlers who competed at the World Championship held in Belgrade, Serbia, participated in this research. All respondents completed a questionnaire designed to evaluate RWL patterns in combat athletes. Participants were classified according to wrestling style: Greco-Roman, freestyle, and women wrestling. Results: Sixty-nine percent of wrestlers had previously lost weight to compete. Most respondents start losing weight approximately seven days before a competition. Athletes reported that they commonly reduced 3.84 ± 2.82 kg to reach the target weight. The wrestling coach represents the most influential person in terms of RWL strategies, while nutritionists and physicians have the least impact on the weight-cutting process. Regarding the methods applied, differences between all the three styles were found in the following variables: training in a heated room, restricting fluid ingestion, training in plastic suits, gradual dieting, increased exercise, diet pills, and sauna. The most frequently used techniques were increased exercise, gradual dieting, training in a heated room, and sauna for all competitors. Diet pills, diuretics, laxatives, and vomiting were the least implemented methods. Conclusions: The obtained results suggest that most wrestlers practice RWL despite the harmful health effects. The education of wrestling coaches is necessary in order to control and decrease the negative impact of RWL.

The Applicability of Provocative Functional Tests in the Diagnosis of Rotator Cuff Muscle Injuries of the Best University Athletes.

Rotator cuff disease, external and internal impingement syndromes, low shoulder stability, various types of trauma, and overuse injuries are all related to sports activities. In order to check symptoms in patients with disability and shoulder pain, clinicians use different methods and diagnostic imaging assessment. The research is aimed at evaluating whether there is a difference between provocation function tests (PFT) and ultrasonographic (US) testing of muscles within the rotator cuff in elite collegiate athletes. Patients (n = 184) were recruited from university team sports selections and tested with a standardized US examination of the shoulder and five PFTs (Speed's test, Neer's test, Hawkins test, lift-off test, Yergason's test). Based on the VAS pain assessment scale, 60 subjects had some pain, which was taken for further processing in the work (124 subjects did not have the presence of pain and were excluded from further processing). The US examination was conducted using Voluson 730 apparatus, by a linear probe, with the frequency in the range of 6-12 MHz. The chi-square test showed significant differences between PFT and the occurrence of shoulder muscle tendinitis for the following variables: Speed's test and subscapularis tendinitis (p = 0.02) and Speed's test and infraspinatus tendinitis (p = 0.01); Neer test and biceps brachii caput longum tendinitis (p = 0.01), Neer test and supraspinatus tendinitis (p = 0.02) and Neer test and infraspinatus tendinitis (p = 0.01); lift-off test and subscapularis tendinitis (p = 0.05); and Yergason's test and biceps brachii caput longum tendinitis (p = 0.03) and Yergason's test and subscapitis tendinitis (p = 0.01). The greatest effect of differences was observed in Neer's test and biceps brachii caput longum tendinitis (φ = 0.60), while the other effects can be described as medium and small in most cases. It can be concluded that functional tests are good predictors of soft tissue changes in the muscles of the rotator cuff of the shoulder. Further monitoring and analysis are needed on a larger number of athletes.

Epidemiology of children's swimming competence and water safety.

Introduction: The main purpose of this study was to investigate children's swimming competence in primary schools of districts in Vojvodina, Serbia. Methods: Included subjects were primary school students from first to eighth grade (N = 2,778; male = 1,454, female = 1,324; age = 10.73 ± 2.1 years). We used Swimming Competence Questionnaire to acquire and analyze their swimming experience, non-fatal aquatic events, and demographics. For the statistical analysis, logistic regression and hierarchical multiple regression were used to evaluate if the factors and SC and NFAE were associated. The analyses were carried out by using SPSS® software version 24.0 (SPSS, Inc., Chicago, Illinois, USA). Results: Families with more income and education generally have children with more swimming competence, experience, knowledge, and skills related to water safety. First step in analysis revealed that gender (ß = 0.05, p < 0.01), education level (ß = 0.06, p < 0.01) age (ß = 0.171, p < 0.01), and family income (ß = 0.04, p < 0.01) were significant swimming competence (SC) predictors (R2 = 0.04). Age (OR = 1.15, p < 0.01) was the only significant predictor in Step 1 predicting non-fatal aquatic events (NFAE). In Step 2, variables associated with SC were swimming location (ΔR2 = 0.06, p < 0.01), swimming experience (ΔR2 = 0.16, p < 0.01), swimming accessibility (ΔR2 = 0.05, p < 0.01), and learning experience (ΔR2 = 0.03, p < 0.01) (total R2 = 0.26 to 0.47, p < 0.01). Only a minority of participants reported that they could not swim further than 5 meters using general stroke (37.15%). Conclusion: National education trainers programs must be prioritized with the primary strategy of transferring knowledge to swimming and water safety. Families with lower income must be included without exceptions. This is perhaps a key factor in preventing NFAE, increasing SC, and increasing water safety.

Let's (Tik) Talk About Fitness Trends.

Several factors that follow the development of society affect physical inactivity, which primarily includes the development of technology and digitalization and the increasing choice of unhealthy lifestyle habits. However, certain shifts in the fitness industry have been noted in the last decade. The development of wearable technologies and artificial intelligence is one of the leading fitness trends and undoubtedly represents the future of the fitness industry. On the other hand, the significant influence of social media and networks affects the development and attitudes of people related to physical activity. Therefore, this review paper evaluates the advantages and disadvantages of wearable technologies and artificial intelligence, the positive and negative effects of social networks, and points out the problems accompanying these new fitness trends. The development of fitness trends follows humanity's needs, and one of the biggest challenges is incorporating these novelties in a mission to improve physical activity levels worldwide.

Rapid Weight Loss Practices in Grapplers Competing in Combat Sports.

BACKGROUND: Grappling is a wrestling style that combines different techniques such as freestyle wrestling, jiu-jitsu, judo, sambo, and others. As with other combat sports, it requires categorizing the athletes in weight classes, which leads to the use of certain methods to lose body weight in a short amount of time which poses a serious threat to athletes' health and wellbeing. Therefore, the objective of this study was to investigate the most widespread rapid weight loss (RWL) methods and sources of influence used by grappling athletes. METHODS: A total of 145 athletes took part in the study by voluntarily filling out a questionnaire regarding their weight loss techniques and methods. They were divided into two groups, male (27.7 ± 5.2 years, 1.76 ± 0.13 m, and 82.1 ± 20 kg) and female (27.33 ± 6.3 years, 1.65 ± 0.08 m, and 64.3 ± 10.4 kg), for further statistical analysis. After calculating descriptive statistics for all the variables, a t-test was conducted for gender differences in weight loss and weight regain, and a chi-square test measured the diversity in techniques and source of influence. RESULTS: Out of 145 participants, 120 athletes (85.2%) reported engaging in rapid weight loss prior to weigh-in. Coaches (52.4 and 59%) and teammates (42.6 and 22.1%) seemed to be the most influential on their rapid weight-loss strategies, whereas physicians (17.1 and 17.9%) and parents (23.2 and 23.1%) were the least influential. A statistical difference between men and women (p = 0.05) was found when teammates were a source of influence (42.6 and 21.1%, respectively). Regarding the methods used, both groups practiced gradual dieting (85.4 and 79.5%) as the most prevalent, followed by increased exercise (79 and 66.6%) and sauna (78.7 and 66.6%). Moreover, men trained in plastic suits significantly more often than women (67.1 vs. 41%, p = 0.01). CONCLUSION: Rapid weight loss is detrimental to athletes' health and wellbeing. Hence, it is crucial to find and implement methods that will control and ultimately limit its use in combat sports. Physicians and nutritionists need to be closely linked with the staff, collaborate and supervise the weight cutting.

Guanidinoacetate-creatine in secondary progressive multiple sclerosis: a case report.

Acute secondary progressive multiple sclerosis (SPMS) is characterized by escalating neurological disability, with limited disease-modifying therapeutic options. A 48-year-old woman with acute SPMS being treated with interferon beta-1a and oral corticosteroids presented as a clinical outpatient with no disease-modifying effects after treatment. A decision was made to treat her with a combination of guanidinoacetate and creatine for 21 days. She had made clinical progress at follow-up, with the intensity of fatigue dropping from severe to mild. Magnetic resonance spectroscopy revealed increased brain choline, creatine, N-acetylaspartate, and glutathione. Patients with SPMS may benefit from guanidinoacetate-creatine treatment in terms of patient- and clinician-reported outcomes; this requires additional study.

Cardiovascular autonomic reflex tests and serum FGF21 levels in overweight and normal-weight men and women.

OBJECTIVE: We evaluated cardiovascular autonomic reflexes and serum fibroblast growth factor 21 (FGF21), a surrogate marker of mitochondrial function, in a cohort of overweight and normal-weight adults (n = 42). METHODS: Indices of autonomic function were monitored during supine rest, autonomic reflex tests and submaximal clinical exercise test, with heart rate variables and blood pressure measured with an automatic system. RESULTS: Markers of sympathetic dominance were accentuated in overweight adults, including elevated resting low-frequency to the high-frequency ratio for heart rate variability (203 ± 227 vs. 96 ± 42; p = .01), and handgrip diastolic blood pressure (36 ± 15 mmHg vs. 25 ± 12 mmHg; p = .01). A weak non-significant trend has been found for a negative correlation between blood pressure responses to isometric handgrip test and FGF21 in the overweight group (r = -0.37; p = .09). CONCLUSIONS: Excess body weight appears to trigger sympathetic overactivity in overweight adults, yet autonomic dysregulation might not be associated with notable changes in serum FGF21.