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1.
Sci Rep ; 9(1): 11196, 2019 08 01.
Artículo en Inglés | MEDLINE | ID: mdl-31371749

RESUMEN

In plants, low-dose of exogenous bacterial cyclic lipopeptides (CLPs) trigger transient membrane changes leading to activation of early and late defence responses. Here, a forward chemical genetics approach identifies colistin sulphate (CS) CLP as a novel plant defence inducer. CS uniquely triggers activation of the PATHOGENESIS-RELATED 1 (PR1) gene and resistance against Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) in Arabidopsis thaliana (Arabidopsis) independently of the PR1 classical inducer, salicylic acid (SA) and the key SA-signalling protein, NON-EXPRESSOR OF PR1 (NPR1). Low bioactive concentration of CS does not trigger activation of early defence markers such as reactive oxygen species (ROS) and mitogen activated protein kinase (MAPK). However, it strongly suppresses primary root length elongation. Structure activity relationship (SAR) assays and mode-of-action (MoA) studies show the acyl chain and activation of a ∼46 kDa p38-like kinase pathway to be crucial for CS' bioactivity. Selective pharmacological inhibition of the active p38-like kinase pathway by SB203580 reverses CS' effects on PR1 activation and root length suppression. Our results with CS as a chemical probe highlight the existence of a novel SA- and NPR1-independent branch of PR1 activation functioning via a membrane-sensitive p38-like kinase pathway.


Asunto(s)
Proteínas de Arabidopsis/genética , Arabidopsis/inmunología , Colistina/metabolismo , Regulación de la Expresión Génica de las Plantas/inmunología , Enfermedades de las Plantas/inmunología , Arabidopsis/genética , Arabidopsis/metabolismo , Arabidopsis/microbiología , Proteínas de Arabidopsis/inmunología , Proteínas de Arabidopsis/metabolismo , Bioensayo , Colistina/química , Sistema de Señalización de MAP Quinasas/genética , Sistema de Señalización de MAP Quinasas/inmunología , Estructura Molecular , Enfermedades de las Plantas/genética , Enfermedades de las Plantas/microbiología , Plantas Modificadas Genéticamente , Pseudomonas syringae/inmunología , Ácido Salicílico/metabolismo , Relación Estructura-Actividad
2.
ACS Chem Biol ; 12(6): 1466-1471, 2017 06 16.
Artículo en Inglés | MEDLINE | ID: mdl-28379676

RESUMEN

Plant growth regulating properties of brevicompanines (Brvs), natural products of the fungus Penicillium brevicompactum, have been known for several years, but further investigations into the molecular mechanism of their bioactivity have not been performed. Following chemical synthesis of brevicompanine derivatives, we studied their activity in the model plant Arabidopsis by a combination of plant growth assays, transcriptional profiling, and numerous additional bioassays. These studies demonstrated that brevicompanines cause transcriptional misregulation of core components of the circadian clock, whereas other biological read-outs were not affected. Brevicompanines thus represent promising chemical tools for investigating the regulation of the plant circadian clock. In addition, our study also illustrates the potential of an unbiased -omics-based characterization of bioactive compounds for identifying the often cryptic modes of action of small molecules.


Asunto(s)
Productos Biológicos/farmacología , Ritmo Circadiano/efectos de los fármacos , Indoles/farmacología , Péptidos Cíclicos/farmacología , Raíces de Plantas/crecimiento & desarrollo , Arabidopsis/efectos de los fármacos , Arabidopsis/fisiología , Productos Biológicos/síntesis química , Indoles/síntesis química , Penicillium/química , Péptidos Cíclicos/síntesis química , Fenómenos Fisiológicos de las Plantas/efectos de los fármacos , Raíces de Plantas/efectos de los fármacos , Transcripción Genética/efectos de los fármacos
3.
Virus Res ; 145(1): 48-53, 2009 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-19540278

RESUMEN

Post-transcriptional gene silencing (PTGS) degrades RNA in a sequence-specific manner and is utilised by plants as a natural defence mechanism against virus invaders. Two members of the genus Crinivirus have been reported to encode suppressors and counter PTGS: Sweet potato chlorotic stunt virus p22 and Tomato chlorosis virus (ToCV) p22, coat protein and coat protein minor. Using an Agrobacterium-mediated transient assay on Nicotiana benthamiana wildtype and 16c plants, we screened four Cucurbit yellow stunting disorder virus (CYSDV) RNA 1-encoded proteins (papain-like protease, p25, p5.2 and p22) to determine which one possess PTGS suppressor activity. Amongst these proteins, only CYSDV p25 was able to suppress (double- and single-stranded) RNA-induced silencing of the green fluorescent protein (GFP) mRNA. Restoration of GFP expression by CYSDV p25 in both of these experiments had no apparent effect on the accumulation of the small interfering RNAs. The identification of CYSDV p25 adds to the list of suppressors encoded by crinivirus RNA 1 molecules, which are unrelated in terms of amino acid sequence homology suggesting distinct PTGS suppression mechanisms and possible roles in viral replication.


Asunto(s)
Crinivirus/genética , Nicotiana/genética , Enfermedades de las Plantas/genética , Interferencia de ARN , Proteínas Virales/metabolismo , Crinivirus/metabolismo , Genes Virales , Proteínas Fluorescentes Verdes , Interacciones Huésped-Patógeno , Enfermedades de las Plantas/virología , ARN Interferente Pequeño/metabolismo , ARN Viral/genética , ARN Viral/metabolismo , Nicotiana/metabolismo , Nicotiana/virología , Proteínas Virales/genética
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