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Arctic regions are extremely sensitive to global warming. Aerosols are one of the most important short-lived climate-forcing agents affecting the Arctic climate. The present study examines the summertime chemical characteristics and potential sources of various organic and inorganic aerosols at a Norwegian Arctic site, Ny-Ålesund (79°N). The results show that organic matter (OM) accounts for 60 % of the total PM10 mass, followed by sulfate (SO42-). Water-soluble organic carbon (WSOC) contributes 62 % of OC. Photochemical processes involving diverse anthropogenic and biogenic precursor compounds are identified as the major sources of WSOC, while water-insoluble organic carbon (WIOC) aerosols are predominantly linked to primary marine emissions. Despite being a remote pristine site, the aerosols show a sign of chemical aging, evidenced by a significant chloride depletion, which was about 82 % on average during the study period. Nitrogen-containing aerosols are likely stemming from migratory seabird colonies and local dust sources around the sampling site. While biogenic, crustal, and sea salt-derived SO42- account for 37%, 8%, and 5% respectively, the remaining 50% is attributed to anthropogenic SO42-. Through chemical tracers, Pearson correlation coefficient matrix, and Hierarchical Cluster Analysis (HCA), the present study identifies soil biota (terrestrial biogenic) and marine emissions, along with their photochemical oxidation processes, as potential sources of Arctic aerosols during summer, while biomass burning and combustion-related sources have a minor contribution. The chemical closure of hygroscopicity highlights that while organics predominantly control aerosol hygroscopicity in the Arctic summer, specific inorganic components like (NH4)2SO4 can significantly increase it on certain days, affecting aerosol-cloud interactions and climate processes over the Arctic during summer. The present study highlights the high abundance of organics and their vital role in the Arctic climate during summer when natural aerosols are conquered.
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Commercially available titanium (Ti) having high mechanical strength and a low area of cross-section can be adequately exploited for minimally invasive dental implantation. Current directions in clinical dental implant therapy focus on endosseous dental implant surfaces with nanoscale topographies using easy and economical processing approaches. The present study describes the generation of a novel nanolayer nucleating agent on the surface of Ti implant for early endosseous after implantation. The strategy is to modify the surface of Ti implant using Ca(OH)2 via hydrothermal technique (Ti-HT). The X-ray photoelectron spectroscopy analysis confirmed the presence of chemically bonded Ca ions on the Ti surface in the form of CaTiO3. In vitro studies are carried out to confirm the bone bonding ability of calcium enriched Ti surface. The apatite deposition on the surface after exposure to SBF for 7â¯days is confirmed via scanning electron microscopy, X-ray powder diffraction, Fourier-transform infrared spectroscopy and energy-dispersive X-ray spectroscopy techniques. The cell viability of Ti-HT was evaluated using direct contact method and MTT assay. The potential of Ca2+ ion on Ti surface via hydrothermal pre-treatment to enhance osseointegration of Ti has been proposed for achieving early stability for dental implants.
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Apatitas/farmacología , Oseointegración/efectos de los fármacos , Temperatura , Titanio/farmacología , Agua/química , Animales , Muerte Celular/efectos de los fármacos , Línea Celular , Ratones , Espectroscopía de Fotoelectrones , Espectroscopía Infrarroja por Transformada de Fourier , Propiedades de Superficie , Difracción de Rayos XRESUMEN
This study presents a characterization of aerosol columnar properties measured at a semi-arid station Anantapur in the southern part of India during the period from October 2012 to September 2013. Aerosol optical depth (AOD) and Angstrom exponent (α) have been retrieved from Microtops II Sunphotometer over the observation site. The results show that a pronounced spectral and monthly variability in the optical properties of aerosols is mainly due to anthropogenic sources. The results show that the spectral curvature can effectively be used as a tool for aerosol type discrimination, since the fine-mode aerosols exhibit negative curvature, while the coarse-mode particles are positive. The classification of aerosols is also proposed by using the values of AOD at 500 nm and Angstrom exponent values (α(380-870)) by applying threshold values obtained from the frequency distribution of AOD. The results of the analysis were identified by four individual components (anthropogenic/biomass burning, coarse/dust, coarse/marine, clean continental) of different origin and compositions. The most frequent situations observed over the site are that due to the anthropogenic/biomass burning situations which account for about 45.37%, followed by coarse/dust (43.64%), clean continental (7.2%) and coarse/marine (3.82%) during summer. The identification of the aerosol source type and the modification processes are analyzed by using the Gobbi et al. (2007) classification scheme based on the measured scattering properties (α, dα) derived from the Microtops II Sunphotometer.
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Aerosoles/análisis , Contaminantes Atmosféricos/análisis , Monitoreo del Ambiente , Atmósfera/química , India , Estaciones del AñoRESUMEN
Calcium sulfate cement (CSC) has emerged as a potential bone filler material mainly because of the possibility of incorporating therapeutic agents. Delivery of the cement through a needle or cannula will make it more useful in clinical applications. However, it was not possible to make CSC injectable because of the inherent lack of viscosity. The present work demonstrates the design development of a viscous and fully-injectable CSC by incorporating hydrogen orthophosphate ions, which does not hamper the biocompatibility of the material. The effect of addition of hydrogen orthophosphate on the rheological properties of the CSC paste was studied using a custom made capillary rheometer. The physicochemical changes associated with cement setting process were examined using X-ray diffraction and Fourier transform infrared spectroscopy and the thermal changes were measured through isothermal differential scanning calorimetry. Micromorphological features of different compositions were observed in environmental scanning electron microscopy and the presence of phosphate ions was identified with energy dispersive X-ray spectroscopic analysis and inductively coupled plasma-optical emission spectroscopy. The results indicated that HPO4 (2-) ions have profound effects on the rheological properties and setting of the CSC paste. Significant finding is that the HPO4 (2-) ions are getting substituted in the calcium sulfate dihydrate crystals during setting. The variations of setting time and compressive strength of the cement with the additive concentration were investigated. An optimum concentration of 2.5 % w/w gave a fully-injectable cement with clinically relevant setting time (below 20 min) and compressive strength (12 MPa). It was possible to inject the optimised cement paste from a syringe through an 18-gauge needle with thumb pressure. This cement will be useful both as bone filler and as a local drug delivery medium and it allows minimally invasive bone defect management.
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Materiales Biocompatibles/química , Sustitutos de Huesos/química , Hidrógeno/química , Fosfatos/química , Cementos para Huesos , Huesos/efectos de los fármacos , Sulfato de Calcio/química , Rastreo Diferencial de Calorimetría , Fuerza Compresiva , Sistemas de Liberación de Medicamentos , Geles , Humanos , Iones , Ensayo de Materiales , Microscopía Electrónica , Agujas , Fósforo/química , Polvos , Presión , Reología , Espectroscopía Infrarroja por Transformada de Fourier , Estrés Mecánico , Viscosidad , Difracción de Rayos XRESUMEN
Chondrosarcoma is a rare malignant cartilaginous tumour of the bone. It commonly occurs in the pelvis, scapula proximal femur, and shoulder girdle .We present a case of a woman in her 56 years of age with chondrosarcoma of the calcaneum-a rare lesion that accounts for 0.5-2.97% of all chondrosarcomas of other sites. Treatment for chondrosarcoma is generally wide surgical excision. Chemotherapy or radiation is not effective for most of these lesions.
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Neoplasias Óseas/patología , Calcáneo/patología , Condrosarcoma/patología , Amputación Quirúrgica , Neoplasias Óseas/cirugía , Calcáneo/cirugía , Condrosarcoma/cirugía , Femenino , Humanos , Persona de Mediana Edad , Dolor/etiología , Enfermedades Raras/patología , Enfermedades Raras/cirugíaRESUMEN
This study was undertaken to glean preliminary information on the role of triphasic ceramic coated hydroxyapatite (HASi) and biphasic (alpha-tricalcium phosphate and hydroxyapatite based) calcium phosphate (BCP) for the development of osteochondral constructs. The proposed constructs were tested for performance in vitro with rabbit adipose-derived mesenchymal stem cells (RADMSCs) and further analysed in vivo in a lapine model for osteochondral defect amelioration. Desirable scaffolding architecture ensuring favourable conditions for cell attachment, nutrient exchange and neo-tissue organization was achieved by the synthesis of porous ceramic blocks and characterizations were carried out using x-ray diffraction and Fourier transform infrared spectroscopy. The cytocompatibility of the scaffold-cell combination product was evaluated using microscopy techniques that proved the scaffold to be non-cytotoxic and favourable for cell growth and proliferation. Short-term implantation studies were conducted with bare cylindrical HASi and BCP scaffolds, press fit deep into the bony bed of the median femoral condyles of the rabbit, which resulted in favourable specific in vivo response of de novo cartilage-like cells on the surface and sub-surface bony trabeculae. The generated pilot data will help to assess the severity of proposed procedures before embarking on scaled-up efforts.
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Cerámica/química , Fracturas del Fémur/patología , Fracturas del Fémur/cirugía , Osteogénesis , Andamios del Tejido , Animales , Curación de Fractura , Diseño de Prótesis , Conejos , Resultado del TratamientoRESUMEN
PURPOSE: The purpose of our study was to fabricate in-house web-camera based automatic continuous patient movement monitoring device and control the movement of the patients during EXRT. METHODS: Web-camera based patient movement monitoring device consists of a computer, digital web-camera, mounting system, breaker circuit, speaker, and visual indicator. The computer is used to control and analyze the patient movement using indigenously developed software. The speaker and the visual indicator are placed in the console room to indicate the positional displacement of the patient. RESULTS: Studies were conducted on phantom and 150 patients with different types of cancers. Our preliminary clinical results indicate that our device is highly reliable and can accurately report smaller movements of the patients in all directions. The results demonstrated that the device was able to detect patient's movements with the sensitivity of about 1 mm. When a patient moves, the receiver activates the circuit; an audible warning sound will be produced in the console. Through real-time measurements, an audible alarm can alert the radiation technologist to stop the treatment if the user defined positional threshold is violated. Simultaneously, the electrical circuit to the teletherapy machine will be activated and radiation will be halted. CONCLUSIONS: Patient's movement during the course for radiotherapy was studied. The beam is halted automatically when the threshold level of the system is exceeded. By using the threshold provided in the system, it is possible to monitor the patient continuously with certain fixed limits. An additional benefit is that it has reduced the tension and stress of a treatment team associated with treating patients who are not immobilized. It also enables the technologists to do their work more efficiently, because they don't have to continuously monitor patients with as much scrutiny as was required.
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Regular measurements of size segregated as well as total mass concentration and size distribution of near surface composite aerosols, made using a ten-channel Quartz Crystal Microbalance (QCM) cascade impactor during the period of September 2007-May 2008 are used to study the aerosol characteristics in association with the synoptic meteorology. The total mass concentration varied from 59.70+/-1.48 to 41.40+/-1.72 microg m(-3), out of which accumulation mode dominated by approximately 50%. On a synoptic scale, aerosol mass concentration in the accumulation (submicron) mode gradually increased from an average low value of approximately 26.92+/-1.53 microg m(-3) during the post monsoon season (September-November) to approximately 34.95+/-1.32 microg m(-3) during winter (December-February) and reaching a peak value of approximately 43.56+/-1.42 microg m(-3) during the summer season (March-May). On the contrary, mass concentration of aerosols in the coarse (supermicron) mode increased from approximately 9.23+/-1.25 microg m(-3)during post monsoon season to reach a comparatively high value of approximately 25.89+/-1.95 microg m(-3) during dry winter months and a low value of approximately 8.07+/-0.76 microg m(-3) during the summer season. Effective radius, a parameter important in determining optical (scattering) properties of aerosol size distribution, varied between 0.104+/-0.08 microm and 0.167+/-0.06 microm with a mean value of 0.143+/-0.01 microm. The fine mode is highly reduced during the post monsoon period and the large and coarse modes continue to remain high (replenished) so that their relative dominance increases. It can be seen that among the two parameters measured, correlation of total mass concentration with air temperature is positive (R(2)=0.82) compared with relative humidity (RH) (R(2)=0.75).
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Aerosoles/análisis , Aerosoles/química , Geografía , Humedad , India , Tamaño de la Partícula , Lluvia , Estaciones del Año , Temperatura , Clima Tropical , VientoRESUMEN
To establish/develop an assay method for measuring Ornithine Aminotransferase (EC.2.6.1.13) activity using rat brain mitochondria as a source of enzyme in presence and absence of Pyridoxal Phosphate (PLP). The modified method, with the improved sensitivity, is adopted for the assay of ornithine amino transferase activity in rat brain mitochondria. The enzyme activity was measured at 620 nm, the study showed that reaction was optimum at 37°C for 30 minutes. The assay is sensitive enough to detect activity at the order of nanomoles pyrroline-5-carboxylate/mg protein/minute and can be compared as an alternative to the radio isotopic method which is more cumbersome and aminobenzaldehyde method which is less sensitive. The K(m) & V(max) shows maximum activity in the presence of Pyridoxal Phosphate (Coenzyme) concentration at 0.05mM when compared with absence of Pyridoxal Phosphate as higher the concentration of Pyridoxal Phosphate affects the affinity of the enzyme to substrate. The OAT activity in different tissues of the rat was also studied and highest activity was found in liver and kidney.
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Scaffolds which encourage the incorporation of a cell source for tissue engineering applications are critical determinants for clinical defects. Over the years, a number of biomaterials have emerged for cell support and growth, but only a few have demonstrated clinical efficacy. We therefore investigated an in-house-developed silica-based bioactive ceramic for its ability to support and sustain the growth of bone marrow-derived mesenchymal stem cells (BMSCs) in vitro. For this, MSCs aspirated from goat bone marrow were isolated and culture expanded on a novel triphasic ceramic composite coated hydroxyapatite (HASi) scaffold comprising hydroxyapatite, tricalcium phosphate and calcium silicate. The viability of cells that harbored on and within the material was ensured through fluorescence-activated cell sorting and confocal laser scanning microscope and for their anchorage sites by scanning electron microscopy. Interestingly, over the days in culture, cell-cell interactions gradually morphed into woven cell-sheets that spanned across the surface of the HASi, forming a canopy. To conclude, we have attempted to carry out the preliminary cytocompatibility studies of this novel ceramic to establish its appropriateness for bone tissue engineering application which is an important criterion in orthopaedic transplantation and regenerative surgery.
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Materiales Biocompatibles/química , Cerámica/química , Durapatita/química , Ingeniería de Tejidos , Animales , Proliferación Celular , Células Cultivadas , Cabras , Células Madre Mesenquimatosas/citología , Células Madre Mesenquimatosas/metabolismo , Microscopía Electrónica de Rastreo , PorosidadRESUMEN
In India the struggle to conquer the diseases began centuries and centuries ago and it is as old as human civilisation. It can be safely presumed that during Vedic period diseases like Ardita vata, Pakshaghata etc. were prevalent. The Brhat Trai, composed during samhita period have described Ardita Vata Vyadhi. The Laghu Trai occupy the next place after Samhitas. Ardita has been mentioned in these works. Other popular texts like Kashyaa Samhita and Bhela Samhita also contain the references about this disease. Modern medicine noticed this disease as facial paralysis at a very later period in the early 18th century.