Dissecting the MUC5AC/ANXA2 signaling axis: implications for brain metastasis in lung adenocarcinoma.

Non-small cell lung carcinoma (NSCLC) exhibits a heightened propensity for brain metastasis, posing a significant clinical challenge. Mucin 5ac (MUC5AC) plays a pivotal role in the development of lung adenocarcinoma (LUAD); however, its role in causing brain metastases remains unknown. In this study, we aimed to investigate the contribution of MUC5AC to brain metastasis in patients with LUAD utilizing various brain metastasis models. Our findings revealed a substantial increase in the MUC5AC level in LUAD brain metastases (LUAD-BrM) samples and brain-tropic cell lines compared to primary samples or parental control cell lines. Intriguingly, depletion of MUC5AC in brain-tropic cells led to significant reductions in intracranial metastasis and tumor growth, and improved survival following intracardiac injection, in contrast to the observations in the control groups. Proteomic analysis revealed that mechanistically, MUC5AC depletion resulted in decreased expression of metastasis-associated molecules. There were increases in epithelial-to-mesenchymal transition, tumor invasiveness, and metastasis phenotypes in tumors with high MUC5AC expression. Furthermore, immunoprecipitation and proteomic analysis revealed a novel interaction of MUC5AC with Annexin A2 (ANXA2), which activated downstream matrix metalloproteases and facilitated extracellular matrix degradation to promote metastasis. Disrupting MUC5AC-ANXA2 signaling with a peptide inhibitor effectively abrogated the metastatic process. Additionally, treatment of tumor cells with an astrocyte-conditioned medium or the chemokine CCL2 resulted in upregulation of MUC5AC expression and enhanced brain colonization. In summary, our study demonstrates that the MUC5AC/ANXA2 signaling axis promotes brain metastasis, suggesting a potential therapeutic paradigm for LUAD patients with high MUC5AC expression.

Royal advertising toothpaste pot lids.

Royal patronage is not something new. Engraved images of British royalty were used by early toothpaste manufacturers, dentists and perfumiers to convey royal endorsement to boost their product sales in the nineteenth and early twentieth centuries. Packaged in high-quality ceramic pots, these toothpastes and powders promoted cleaning, beautifying and preserving the teeth and gums. These fascinating containers, sealed with a transfer-printed lid, feature inventive designs and typefaces and highlight sophisticated manufacturing and marketing skills to entice consumers. Many have been re-discovered from the excavation of former rubbish tips and are often the only tangible evidence of long defunct businesses that elected to use this latest form of packaging.

The Most Reliable Remedy for Man or Beast Ever Before the Public!

A unique "miracle cure-all" developed for both Man or Beast that was available from the mid-1800s into the 20 century is highlighted with some interesting anecdotal perspectives.

19th Century Deciduous Tooth Boxes from France and Belgium.

An encounter 50 years ago led to an interesting collection of tooth boxes. This paper will highlight many of those unique treasures emanating from Northwestern Europe.

Unusual Dentists' Advertisements from the 1932 Los Angeles Olympics.

The 1932 Olympiad in Los Angeles was not only transformative to the City of Los Angeles but also successful due to the efforts of several dedicated men. The Games were promoted by selected publicity that involved certain types of print media including stamps produced by the United States Postal Service. Advertising "seals" involved a vast number of businesses and products and all this added up to make the depression era Olympic Games successful. Included in the advertising seals were some participating California dentists. Those known dentists are shown here.

Postcards related to Dentistry: Iron-Jaw Acts and aPromotional Optical Illusion.

Demonstrable feats of strength and valor using the teeth are found throughout many aspects of newsworthy items and social encounters in history past. Likewise, promotional opportunities relative to the dentition were also common. This article addresses many of these sensationalistic scenarios that were found on postcards related to dentistry in the early 1900s.

Dear Sis: An 1844 Letter From a Young Connecticut Dentist.

In 1844, Queen Victoria was 7 years into her 63-year reign of the Queen of the United Kingdom of Great Britain and Ireland. John Tyler was 10th president of the United States, to be followed by the 11th president, by James K. Polk in March of 1845. Four years before, Dr. Horace H. Hayden and Chapin A. Harris co-founded The Baltimore College of Dental Surgery. The school was chartered by an act of the Maryland State Legislature in 1840. Dr. Hayden died on 25 January 1844.

Thomas Shields Henderson - Brooklyn Dentist and Irish Nationalist.

An unusual card advertising the dental services of T.S. Henderson revives the story of an Irish dentist who left his homeland and came to Brooklyn, New York to practice. He was a fervent Irish Nationalist and was active in Irish causes. Henderson was an abuser of alcohol and eventually found dead in Albany, New York. The death was considered a suicide, but was it?

Mechanical Dentistry: George Derby, An Unknown Artist, and Rube Goldberg.

For more than a century, at least, tooth extraction by ingenious mechanical contraptions has piqued the interest and imagination of illustrators, especially cartoonists. George Derby, an unknown British talent, and Ruben Garrett Lucius "Rube" Goldberg all dabbled in oral surgical mechanical humor.

ODOL MUNDVASSER and ZAHNPASTA: Part II - International Success.

In Part I of ODOL MUNDVASSER and ZAHNPASTA: Part I-von Stuck, PUCCINI, and up in the AIR1 we described how in 1892, Dresden's Karl August Lingner (1861-1916), produced and marketed Professor Bruno Richard Seifert's (1861-1919) invention of Odol Mouthrinse, and subsequently Odol Toothpaste. The focus of Part I was how Lingner's Company used "aeronauticak" postcard advertising, ie., dirigibles and airplanes of the times, to advertise their products. A concise summary of the history of Lingner-Werke A.G., Berlin, and what happened to Odol after Lingner's death in 1916, was succinctly reported by Patrick van der Vegt on this website. https://www.atlas-repropaperwork.com/odol-toothpaste/.

Drs. H. Reid Staley and G.M. Brown and Their "Banner Lady" Advertisements.

In the late 1800s ladies were portrayed on "Cabinet Cards" to serve as photographic advertisements that appealed to the needs of the public…in today's world a veritable walking internet display of goods and services.

ODOL MUNDVASSER and ZAHNPASTA: Part I-von Stuck, PUCCINI, and up in the AIR.

Professor Bruno Richard Seifert (1861-1919) (Fig.1), a renowned German chemist, invented a mouthwash that combined an antiseptic with essential oils. (https://second.wiki/wiki/richard_seifert). Karl August Lingner (1861-1916) (Fig. 2), of Dresden, Germany, produced and introduced Seifert's mouthwash in 1892. It was called "ODOL." An excellent review of Lingner's life can be accessed on this website: (https://second.wiki/wiki/karl_august_lingner). Along with ODOL Mundwasser (Mouthwash), ODOL Zahnpasta (Toothpaste) was also produced (Fig. 3). ODOL products are still sold today, 130 years later!

Gehrig Brothers Electo-motor Teething Necklace.

Victorian era patent medicines to soothe discomfort from teething infants worked well because they contained dangerous pain killing drugs such as narcotics and alcohol. Relief from teething discomfort can also be achieved for a teething infant by the baby chewing on various objects such as hard teething rings or teething necklaces.This report offers some historical perspective on infant teething and describes and pictures a well-marketed German teething necklace of the 19th century.

Early Dental Trade Card Metamorphic Before and After.

In the late 18th and early 19th century American-trained dentists were perceived by the public to be superior to locally trained dentists in many parts of Europe. Some individuals traveled to the United States for training. Other dentists simply claimed falsely to have an American degree or received one through the mail from one of several dental school diploma mills operating in the United States.

Victorian Era Preventive and Children's Dentistry, By George!

Dr. George C. Ainsworth and Dr. George F. Emerson were two Boston dentists from the late 19th century who were interested in preventive dentistry, "irregularities" in teeth arrangements, and generally, the oral health care of children. Leaflets from their practices reveal much about their services and concern for all their patients.

Early Dental Trade Card with Unusual Logo.

James Washington Bartlett (1839-1910, FAG Memorial #124715057) was born in Derby Line, Orleans County, Vermont to Nathaniel Bartlett (1791-1866), a harness maker, and Martha Pinkham Bartlett (1798-1847). Little is known about his youth nor his education. He was evidently preceptor trained in dentistry but we were unable to determine with whom.

Early Dental Trade Card Printed on Silk.

The French dentist Désirabode was the author of Nouveaux Éléments Complets De La Science Et De L'art Du Dentiste Théorique Et Practique De L'art Du Dentiste (Complete Elements of the Science and Art of the Dentist) in 1843, in two volumes.

Pediatric Oral Surgery in France, Circa 1904.

In 1904, Mr. Maurice Lemaistre of Bermonville, France received four postcards at his home (Chez Monsieur Lemaistre) from family (Par Famille). The reverse of one of those "CARTE POSTALES" is shown in Figure 1.

Luther Franklin Locke, M.D.: 19th Century Children's Dentistry Advocate.

Charles B. Hammond, M.D., of Nashua, New Hampshire did a favor for today's readers of the Journal of the History of Dentistry. He wrote a concise but fact-filled biography of Luther Franklin Locke, M.D. which was published in Edward Everett Parker's History if the City of Nashua, in 1897.1.