Response of soil heavy metal forms and bioavailability to the application of microplastics across five years in different soil types.

Microplastics (MPs) potentially alter physicochemical and transformation of heavy metals (HMs) in soils, which may depend on the specific characteristics of soil types. However, the dynamical and long-term mechanisms remain to be elucidated. A five-year incubation experiment was conducted to evaluate the influence of MPs on the chemical speciation of Pb, Ni, Cu, Cr, Cd, and As in the meadow, tidal, cinnamon, saline-alkali, and brown soils. From the first year to the fifth year, the clay value of the meadow, tidal, cinnamon, and saline-alkali soils was increased by 31.35 %, 9.63 %, 30.12 %, and 33.12 %, respectively; the pH values of the cinnamon and saline-alkali soils were increased by 15.02 % and 15.86 %, respectively. Besides, speciation distribution results suggested that the application of MPs reduced the liable available (LB) form (F2-dissolved and F3-ion exchangeable) of HMs and increased the potentially available (PB) form (F5-minerals and F6-organic-bound fraction) of HMs in all soils. Compared with other forms, F2 HMs fraction was the most responsive to MPs. Furthermore, the average bioconcentration factor (BCF) of Cr and Pb decreased by 73.75 % and 70.41 % in soils, respectively. Interestingly, soil type showed more impact on the form of HMs, which was associated with the different physicochemical parameters of soils, while application time displayed more impact on the bioavailability of HMs. Moreover, our results suggested that soils with higher clay content and pH values (such as cinnamon and saline-alkali soils) may mitigate the bioavailability of HMs more effectively in the presence of MPs, while soils with lower clay content may be more vulnerable to HMs contamination over time. This work highlights the importance of long-term monitoring of the impact of MPs on HMs dynamics for effective mitigation of soil contamination risks. Our study provides valuable guidance for soil remediation strategies and environmental quality management across different soil types.

Crucial role of humic substance type in Cr(VI) reduction by humic substance-Fe(III) coprecipitates but not in adsorption.

Wetlands exhibit a self-purification effect on Cr(VI) owing to the adsorption and reduction capabilities of their humic substance (HS)-Fe(III) coprecipitates. However, the similarities and differences in the adsorption and reduction of Cr(VI) by different types of HS-Fe(III) coprecipitates remains unknown. In this study, fulvic acid (FA)- and humic acid (HA)-Fe(III) coprecipitates were synthesized with initial C/Fe ratios ranging from 0.25 to 15, and a comparative analysis of their adsorption and reduction effects was conducted. The results showed that FA- and HA-Fe(III) coprecipitates exhibit similar abilities to adsorb Cr(VI) by forming inner-sphere complexes with ferrihydrite (Fh), as well as with FA/HA. The association between FA/HA and Fe(III) not only blocked certain FeOH adsorption sites, but also enhanced the electrostatic repulsion towards Cr(VI), resulting in a proportionate decrease in their adsorption ability. For reduction, inductive and intrinsic reduction were involved in both FA- and HA-Fe(III) coprecipitates. However, the synergism/antagonism differed with the inherent sites (e.g., phenolic hydroxyl, ArOH) and activated sites (e.g., alcoholic hydroxyl, AlOH). Based on the size differences between FA and HA, the AlOH contained in FA was more easily activated by Fe(III) than that in HA, owing to the shorter induction path required. Thus, consistent synergism was observed in FA-Fe(III) coprecipitates regardless of Fe(III) species. In contrast, synergism was only observed in HA-Fe(III) coprecipitates containing Fh, because the inductive ability of Fe(III) cations was too weak to activate AlOH through the longer path. Moreover, with irreversible consumption of AlOH, intrinsic reduction became the dominant pathway at concentrations >1 mM Cr(VI). The preferential elimination of ArOH subsequently led to a transition to antagonism. These outcomes deepen our scientifical understanding of the environmental effects of HS-Fe(III) coprecipitates and offer new perspectives for exploring their potential applications in the remediation of Cr-contaminated sites.

Insights into the critical roles of water-soluble organic matter and humic acid within kitchen compost in influencing cadmium bioavailability.

Compost has demonstrated potential as a cadmium (Cd) remediation agent, while it still remains unclear about the core components in driving the bioactive transformation of Cd. To address this issue, this study isolated three components-kitchen compost powder (KC), humic acid (HA), and water-soluble organic matter (DOM)-from kitchen compost to regulate soil properties, bacterial community structures and functions, and Cd migration risks. The results revealed that the addition of 20% KC and HA reduced the bioavailability factor of Cd by 47.20% and 16.74%, respectively, with HA contributing 35.47% of the total reduction achieved with KC. Conversely, the application of DOM increased the Cd risk through a reduction in soil pH and an increase in the abundance of Cd-activating bacteria, which adversely affected the stability of Cd complexes. However, the porous structure and organic matter in KC and HA provided adsorption sites for Cd passivation and promoted the growth of Cd-fixing bacteria. This study effectively identifies both the positive and negative effects of key compost components on Cd migration and provides scientific guidance for applying kitchen compost in soil management.

Applying kitchen compost promoted soil chrysene degradation by optimizing microbial community structure.

Chrysene, as a high molecular weight polycyclic aromatic hydrocarbon (PAH), has become an important factor in degrading soil quality and constraining the safe production of food crops. Compost has been widely used to amend contaminated soil. However, to date, the main components of kitchen compost that enhance the biodegradation of chrysene in the soil remain unidentified. Thus, in this study, the enhancing effect and mechanisms of kitchen compost (KC) and kitchen compost-derived dissolved organic matter (KCOM) on chrysene removal from soil were investigated through cultivation experiments combined with high-throughput sequencing technology. Additionally, the key components influencing the degradation of chrysene were identified. The results showed that KCOM was the main component of compost that promoted the degradation of chrysene. The average degradation rate of chrysene in 1% KC- and 1% KCOM-treated soil increased by 27.20% and 24.18%, respectively, at different levels of chrysene pollution compared with the control treatment (CK). KC and KCOM significantly increased soil nutrient content, accelerated humification of organic matter, and increased microbial activity in the chrysene-contaminated soil. Correlation analyses revealed that the application of KC and KCOM optimized the microbial community by altering soil properties and organic matter structure. This optimization enhanced the degradation of soil chrysene by increasing the abundance of chrysene-degrading functional bacteria from the genera Bacillus, Arthrobacter, Pseudomonas, Lysinibacillus, and Acinetobacter. This study provides insight into the identification of key components that promote chrysene degradation and into the microbial-enhanced remediation of chrysene-contaminated soil.

Aerobic Fe transformation induced decrease in the adsorption and enhancement in the reduction of Cr(VI) by humic acid-ferric iron coprecipitates.

Humic substance (HS)-ferric iron (Fe(III)) coprecipitates are widespread organo-mineral associations in soils and aquifers and have the capacity to immobilize and detoxify Cr(VI). These coprecipitates undergo transformation owing to their thermodynamic instability; however, the effects of this transformation on their environmental behaviors remain unclear, particularly in aerobic environments. In this study, the aerobic transformation of humic acid (HA)-Fe(III) coprecipitates, a representative of HS-Fe(III) coprecipitates, was simulated. The environmental effect was then evaluated after conducting an adsorption-reduction batch experiment toward Cr(VI). The aerobic transformation characteristics, as well as the adsorption/reduction capacity of HA-Fe(III) coprecipitates, were found to depend strongly on their structures. In ferrihydrite (Fh)-like coprecipitates, amorphous Fh is readily transformed into crystalline hematite and goethite at aerobic environments, leading to a much lower specific surface area and adsorption capacity. However, this increasing degree of crystallization enhanced the inductive reduction ability towards Cr(VI) owing to the more significant shift of electron pairs in the FeOC bond toward the HA direction. In HS-like coprecipitates, Fe(III) always serves as a cation bridge connecting HA molecules, but can be reduced to Fe(II) by the associated HA after aerobic transformation. The produced Fe(II), therefore, drove the reduction of the adsorbed Cr(VI). These findings emphasize the pivotal role of aerobic transformation in enhancing the reduction capacity for Cr(VI), which opens a new avenue for the development of in-situ remediation agents for Cr(VI)-contaminated sites.

Differential carbon accumulation of microbial necromass and plant lignin by pollution of polyethylene and polylactic acid microplastics in soil.

The wide microplastics (MPs) occurrence affects soil physicochemical and biological properties, thereby influencing its carbon cycling and storage. However, the regulation effect of MPs on soil organic carbon (SOC) formation and stabilization remains unclear, hindering the accurate prediction of carbon sequestration in future global changes under continuous MP pollution. Phospholipid fatty acids, amino sugars and lignin phenols were used in this study as biomarkers for microbial community composition, microbial necromass and plant lignin components, respectively, and their responses to conventional (polyethylene; PE) and biodegradable (polylactic acid; PLA) MPs were explored. Results showed PLA MPs had positive effects on soil microbial biomass, while the positive and negative effects of PE MPs on microbial biomass varied with MP concentration. PE and PLA MPs increased microbial necromass contents and their contribution to SOC, mainly due to the increase in fungal necromass. On the contrary, PE and PLA MPs reduced lignin phenols and their contribution to SOC, mainly owing to the reduction in vanillyl-type phenols. The response of microbial necromass to PLA MPs was higher than that to PE MPs, whereas the response of lignin phenols was the opposite. MPs increased SOC level, with 83%-200% and 50%-75% of additional SOC in PE and PLA treatments, respectively, originating from microbial necromass carbon. This finding indicates that the increase in SOC pool in the presence of MPs can be attributed to soil microbial necromass carbon, and MPs increased capacity and efficacy of microbial carbon pump by increasing microbial turnover and reducing microbial N limitation. Moreover, the increase in amino sugars to lignin phenols ratio in PE treatment was higher than that in PLA treatment, and the increase in SOC content in PLA treatment was higher than that in PE treatment, indicating a high possibility of SOC storage owing to PLA MPs.

Co-hydrothermal carbonization of municipal sludge and agricultural waste to reduce plant growth inhibition by aqueous phase products: Molecular level analysis of organic matter.

The organic matter molecular mechanism by which combined hydrothermal carbonization (co-HTC) of municipal sludge (MS) and agricultural wastes (rice husk, spent mushroom substrate, and wheat straw) reduces the inhibitory effects of aqueous phase (AP) products on pak choi (Brassica campestris L.) growth compared to HTC of MS alone is not clear. Fourier-transform ion cyclotron resonance mass spectrometry was used to characterize the differences in organic matter at the molecular level between AP from MS HTC alone (AP-MS) and AP from co-HTC of MS and agricultural waste (co-Aps). The results showed that N-bearing molecules of AP-MS and co-Aps account for 70.6 % and 54.2 %-64.1 % of all molecules, respectively. Lignins were present in the highest proportion (56.3 %-78.5 %) in all APs, followed by proteins and lipids. The dry weight of co-APs hydroponically grown pak choi was 31.6 %-47.6 % higher than that of the AP-MS. Molecules that were poorly saturated and with low aromaticity were preferentially consumed during hydroponic treatment. Molecules present before and after hydroponics were defined as resistant molecules; molecules present before hydroponics but absent after hydroponics were defined as removed molecules; and molecules absent before hydroponics but present after hydroponics were defined as produced molecules. Large lignin molecules were broken down into more unsaturated molecules, but lignins were the most commonly resistant, removed, and produced molecules. Correlation analysis revealed that N- or S-bearing molecules were phytotoxic in the AP. Tannins positively influenced the growth of pak choi. These results provide new insights into potential implementation strategies for liquid fertilizers produced from AP arising from HTC of MS and agricultural wastes.

Upgrade from aerated static pile to agitated bed systems promotes lignocellulose degradation in large-scale composting through enhanced microbial functional diversity.

Composting presents a viable management solution for lignocellulose-rich municipal solid waste. However, our understanding about the microbial metabolic mechanisms involved in the biodegradation of lignocellulose, particularly in industrial-scale composting plants, remains limited. This study employed metaproteomics to compare the impact of upgrading from aerated static pile (ASP) to agitated bed (AB) systems on physicochemical parameters, lignocellulose biodegradation, and microbial metabolic pathways during large-scale biowaste composting process, marking the first investigation of its kind. The degradation rates of lignocellulose including cellulose, hemicellulose, and lignin were significantly higher in AB (8.21%-32.54%, 10.21%-39.41%, and 6.21%-26.78%) than those (5.72%-23.15%, 7.01%-33.26%, and 4.79%-19.76%) in ASP at three thermal stages, respectively. The AB system in comparison to ASP increased the carbohydrate-active enzymes (CAZymes) abundance and production of the three essential enzymes required for lignocellulose decomposition involving a mixture of bacteria and fungi (i.e., Actinobacteria, Bacilli, Sordariomycetes and Eurotiomycetes). Conversely, ASP primarily produced exoglucanase and ß-glucosidase via fungi (i.e., Ascomycota). Moreover, AB effectively mitigated microbial stress caused by acetic acid accumulation by regulating the key enzymes involved in acetate conversion, including acetyl-coenzyme A synthetase and acetate kinase. Overall, the AB upgraded from ASP facilitated the lignocellulose degradation and fostered more diverse functional microbial communities in large-scale composting. Our findings offer a valuable scientific basis to guide the engineering feasibility and environmental sustainability for large-scale industrial composting plants for treating lignocellulose-rich waste. These findings have important implications for establishing green sustainable development models (e.g., a circular economy based on material recovery) and for achieving sustainable development goals.

Differential responses of the electron transfer capacities of soil humic acid and fulvic acid to long-term wastewater irrigation.

Wastewater irrigation is used to supplement agricultural irrigation because of its benefits and freshwater resource scarcity. However, whether wastewater irrigation for many years affects the electron transfer capacity (ETC) of natural organic matter in soil remains unclear, and organic matter could influence the decomposition and mineralization of substances with redox characteristics in soil through electron transfer, ultimately affecting the soil environment. The composition of soil humic substances (HS) is highly complex, and the effects of soil humic acid (HA) and fulvic acid (FA) on ETC is poorly understood. In this study, we separately evaluated the responses of the electron-accepting capacity (EAC) and electron-donating capacity (EDC) of soil HA and FA in agricultural fields to various durations of wastewater irrigation. Results showed that the EAC of HA and FA increased significantly with increasing the duration of wastewater irrigation. When wastewater irrigation lasted for 56 years, the EAC of HA showed a higher increment (590 %) than that of FA (223 %). The EDC of soil HA and FA, conversely, decreased compared to the control, with the highest reduction of 35.6 % for HA and 65.9 % for FA. Specifically, the EDC of HA gradually decreased starting from 29 years of wastewater irrigation, whereas the decrease in the EDC of FA exhibited no clear pattern in relation to the duration of wastewater irrigation. Increased soil organic matter and total nitrogen content under long-term wastewater irrigation led to an increase in sucrase and phosphatase activities, along with an increase in EAC and a decrease in EDC of HS. This suggests that soil enzyme activities may ultimately lead to changes in ETC. The results of this research provide practical insights into the redox system in soil and its driving role in soil organic matter transformation and nutrient cycling under wastewater irrigation.

Assessing the impacts and contamination potentials of landfill leachate on adjacent groundwater systems.

Landfilling is a globally prevalent method for managing municipal solid waste disposal. Nonetheless, the potential for serious contamination and the significant regional disparities in the leachate produced pose varying degrees of risks to groundwater quality. Previous studies have focused on a single landfill or the same geo-climatic conditions, with a limited number of samples having resulted in a narrow distribution of landfill age and scale, which prevents the description of the pattern of change in landfill age and scale. As well as the effect of this change on the contaminants in the landfill leachate and surrounding groundwater is still unclear. Therefore, we sampled and analyzed leachate and surrounding groundwater from 62 landfills with different landfill ages, scales, and operating conditions in a region with dense and varied topography and climate. Aim to explore the effects of different landfill ages, scales, and operating conditions on contaminants in leachate and surrounding groundwater. Findings indicate that pollutant profiles in different media are influenced by the age, scale, and operational status of the landfill, and the impact of leachate on pollutant types and concentrations in groundwater is limited. A significant correlation exists between the concentration of contaminants in the groundwater affected by leaching from the impermeable layer and the age and scale of the landfill when compared to the leachate. The contamination potentials posed by different pollutants vary across environmental media. Total dissolved solids and NH4+-N in leachate presented high contamination potentials, whereas elemental metalloids (Mn, Al, Ba, and Fe) in the surrounding groundwater posed high environmental concerns. These insights furnish new avenues for monitoring, identification, and safeguarding against pollutants in landfills and proximate groundwater, which is imperative for the sustainable management of municipal waste.

Effect of aqueous phase from hydrothermal carbonization of sewage sludge on heavy metals and heavy metal resistance genes during chicken manure composting.

Livestock manure is often contaminated with heavy metals (HMs) and HM resistance genes (HMRGs), which pollute the environment. In this study, we aimed to investigate the effects of the aqueous phase (AP) produced by hydrothermal carbonization (HTC) of sewage sludge (SS) alone and the AP produced by co-HTC of rice husk (RH) and SS (RH-SS) on humification, HM bioavailability, and HMRGs during chicken manure composting. RH-SS and SS increased the humic acid content of the compost products by 18.3 % and 9.7 %, respectively, and significantly increased the humification index (P < 0.05) compared to the CK (addition of tap water). The passivation of HMs (Zn, Cu, As, Pb, and Cr) increased by 12.17-23.36 % and 9.74-15.95 % for RH-SS and SS, respectively, compared with that for CK. RH-SS and SS reduced the HMRG abundance in composted products by 22.29 % and 15.07 %, respectively. The partial least squares path modeling results showed that SS and RH-SS promoted compost humification while simultaneously altering the bacterial community and reducing the bioavailability of metals and host abundance of HMRGs, which has a direct inhibitory effect on the production and distribution of HMRGs. These findings support a new strategy to reduce the environmental risk of HMs and HMRGs in livestock manure utilization.

Anaerobic oxidation of methane in landfill and adjacent groundwater environments: Occurrence, mechanisms, and potential applications.

Landfills remain the predominant means of solid waste management worldwide. Widespread distribution and significant stockpiles of waste in landfills make them a significant source of methane emissions, exacerbating climate change. Anaerobic oxidation of methane (AOM) has been shown to play a critical role in mitigating methane emissions on a global scale. The rich methane and electron acceptor environment in landfills provide the necessary reaction conditions for AOM, making it a potentially low-cost and effective strategy for reducing methane emissions in landfills. However, compared to other anaerobic habitats, research on AOM in landfill environments is scarce, and there is a lack of analysis on the potential application of AOM in different zones of landfills. Therefore, this review summarizes the existing knowledge on AOM and its occurrence in landfills, analyzes the possibility of AOM occurrence in different zones of landfills, discusses its potential applications, and explores the challenges and future research directions for AOM in landfill management. The identification of research gaps and future directions outlined in this review encourages further investigation and advancement in the field of AOM, paving the way for more effective waste stabilization, greenhouse gas reduction, and pollutant mitigation strategies in landfills.

Kitchen compost-derived humic acid application promotes ryegrass growth and enhances the accumulation of Cd: An analysis of the soil microenvironment and rhizosphere functional microbes.

Phytoremediation is an environmentally friendly and safe approach for remediating environments contaminated with heavy metals. Humic acid (HA) has high biological activity and can effectively complex with heavy metals. However, whether HA affects available Cd storage and the Cd accumulation ability of plants by altering the soil microenvironment and the distribution of special functional microorganisms remains unclear. Here, we investigated the effects of applying kitchen compost-derived HA on the growth and Cd enrichment capacity of ryegrass (Lolium perenne L.). Additionally, the key role of HA in regulating the structure of rhizosphere soil bacterial communities was identified. HA promoted the growth of perennial ryegrass and biomass accumulation and enhanced the Cd enrichment capacity of ryegrass. The positive effect of HA on the soil microenvironment and rhizosphere bacterial community was the main factor promoting the growth of ryegrass, and this was confirmed by the significant positive correlation between the ryegrass growth index and the content of SOM, AP, AK, and AN, as well as the abundance of rhizosphere growth-promoting bacteria such as Pseudomonas, Steroidobacter, Phenylobacterium, and Caulobacter. HA passivated Cd and inhibited the translocation capacity of ryegrass. The auxiliary effect of resistant bacteria on plants drove the absorption of Cd by ryegrass. In addition, HA enhanced the remediation of Cd-contaminated soil by ryegrass under different Cd levels, which indicated that kitchen compost-derived HA could be widely used for the phytoremediation of Cd-contaminated soil. Generally, our findings will aid the development of improved approaches for the use of kitchen compost-derived HA for the remediation of Cd-contaminated soil.

Transport of per-/polyfluoroalkyl substances from leachate to groundwater as affected by dissolved organic matter in landfills.

The transport of per- and polyfluoroalkyl substances (PFAS) from landfill leachate to surrounding soil and groundwater poses a threat to human health via the food chain or drinking water. Studies have shown that the transport process of PFAS from the solid to liquid phase in the environment is significantly affected by dissolved organic matter (DOM) adsorption. However, the mechanism of PFAS release from landfill solids into leachate and its transport to the surrounding groundwater remains unclear. In this study, we identified the composition of PFAS and DOM components and analyzed the association between DOM components, physicochemical factors, and PFAS concentrations in landfill leachate and groundwater. This study demonstrated that the frequency of PFAS detection in the samples was 100%, and the PFAS concentrations in leachate were greater than in the groundwater samples. Physicochemical factors, such as ammonium-nitrogen (NH4+-N), sodium (Na), calcium (Ca), DOM components C4 (macromolecular humic acid), SUVA254 (aromatic component content), and A240-400 (humification degree and molecular weight), were strongly correlated with PFAS concentrations. In conclusion, PFAS environmental risk management should be enhanced in landfills, especially in closed landfills, or landfills that are scheduled to close in the near future.

Effect of soil organic matter-mediated electron transfer on heavy metal remediation: Current status and perspectives.

Soil contamination by heavy metals poses major risks to human health and the environment. Given the current status of heavy metal pollution, many remediation techniques have been tested at laboratory and contaminated sites. The effects of soil organic matter-mediated electron transfer on heavy metal remediation have not been adequately studied, and the key mechanisms underlying this process have not yet been elucidated. In this review, microbial extracellular electron transfer pathways, organic matter electron transfer for heavy metal reduction, and the factors affecting these processes were discussed to enhance our understanding of heavy metal pollution. It was found that microbial extracellular electrons delivered by electron shuttles have the longest distance among the three electron transfer pathways, and the application of exogenous electron shuttles lays the foundation for efficient and persistent remediation of heavy metals. The organic matter-mediated electron transfer process, wherein organic matter acts as an electron shuttle, promotes the conversion of high valence state metal ions, such as Cr(VI), Hg(II), and U(VI), into less toxic and morphologically stable forms, which inhibits their mobility and bioavailability. Soil type, organic matter structural and content, heavy metal concentrations, and environmental factors (e.g., pH, redox potential, oxygen conditions, and temperature) all influence organic matter-mediated electron transfer processes and bioremediation of heavy metals. Organic matter can more effectively mediate electron transfer for heavy metal remediation under anaerobic conditions, as well as when the heavy metal content is low and the redox potential is suitable under fluvo-aquic/paddy soil conditions. Organic matter with high aromaticity, quinone groups, and phenol groups has a stronger electron transfer ability. This review provides new insights into the control and management of soil contamination and heavy metal remediation technologies.

New insight into the functional group mechanism and structure-activity relationship of the complexation between DOM and Cr(III) in landfill leachate.

Widespread landfills represent a significant source of groundwater contamination. Due to the unique and diverse nature of dissolved organic matter (DOM) in landfill leachate, the interaction between DOM and heavy metals, along with its quantitative evaluation, remains unknown. Consequently, we collected ten samples from various landfill types to serve as representatives for a comprehensive investigation of the mechanism involving functional groups and Cr(III) through the establishment of a quantitative structure-activity relationship (QSAR). We employed ESI FT-ICR MS, (MW) 2D-COS, and DFT calculations for this purpose. Our findings indicate that DOM from landfill leachate contains a higher proportion of CHON molecules on intensity compared to those from natural sources. The maximum complexation capacity was determined by the proportion of proteins (69%), normalized carbon average oxidation state (16%), double bond equivalence (8%), and the number of oxygen atoms (7%) in landfill leachate DOM. Besides, N-containing groups such as N = O and C-N in landfill leachate DOM with lower humification, can exhibit stronger affinities than COOH, ArOH, CO, and polysaccharide C-O groups, which are typically identified as dominant sites in natural DOM. A QSAR model incorporating four parameters demonstrated an impressive accuracy rate of 98.8%, underscoring its reliability in predicting the complexation potential of different landfill leachate DOM with Cr(III).

Antibiotic resistance genes and heavy metals in landfill: A review.

Landfill is reservoir containing antibiotic resistance genes (ARGs) that pose a threat to human life and health. Heavy metals impose lasting effects on ARGs. This review investigated and analyzed the distribution, composition, and abundance of heavy metals and ARGs in landfill. The abundance ranges of ARGs detected in refuse and leachate were similar. The composition of ARG varied with sampling depth in refuse. ARG in leachate varies with the distribution of ARG in the refuse. The ARG of sulI was associated with 11 metals (Co, Pb, Mn, Zn, Cu, Cr, Ni, Sb, As, Cd, and Al). The effects of the total metal concentration on ARG abundance were masked by many factors. Low heavy metal concentrations showed positive effects on ARG diffusion; conversely, high heavy metal concentrations showed negative effects. Organic matter had a selective pressure effect on microorganisms and could provide energy for the diffusion of ARGs. Complexes of heavy metals and organic matter were common in landfill. Therefore, the hypothesis was proposed that organic matter and heavy metals have combined effects on the horizontal gene transfer (HGT) of ARGs during landfill stabilization. This work provides a new basis to better understand the HGT of ARGs in landfill.

Macrogenomic analysis of the effects of aqueous-phase from hydrothermal carbonation of sewage sludge on nitrogen metabolism pathways and associated bacterial communities during composting.

The effects of aqueous phases (AP) formed from hydrothermal carbonation of sewage sludge (with or without rice husk) as moisture regulators of nitrogen metabolism pathways during composting are currently unclear. Macrogenomic analyses revealed that both APs resulted in notably changes in bacterial communities during composting; increased levels of nitrogen assimilation, nitrification, and denitrification metabolic pathways; and decreased levels of nitrogen mineralization metabolic pathways. Genes associated with nitrogen assimilation and mineralization accounted for 34-41% and 32-40% of the annotated reads related to nitrogen cycling during composting, respectively, representing them as the most abundant nitrogen metabolism processes. The gudB and norB were identified as key genes for nitrogen mineralization and nitrous oxide emission, respectively. This research offers a better understanding of the effects of additional nitrogen sources on nitrogen metabolism pathways during composting.

Influence of leachate microenvironment on the occurrence of phthalate esters in landfills.

Phthalate esters (PAEs) are added to various products as plasticizers. Plastic waste containing PAEs enters landfills as they age with use. However, the influence of microenvironmental changes on the occurrence of PAEs during landfill stabilization is still unknown. In this study, we evaluated the relationship between the physical and chemical properties of leachate, the structure of bacterial communities and the chemical structure of dissolved organic matter (DOM), and the occurrence of PAEs and the mechanism underlying their responses to changes. Landfill leachate in different stabilization states had high Cl- and NH4+ contents and its metal element (Cr, Pb, and Zn) contents generally decreased with the increase in landfill ages. Proteobacteria, Bacteroidetes, and Firmicutes were important phyla and had an average relative abundance of 68.63%. The lignin/carboxylate-rich alicyclic molecule structure was the main component of DOM (56%-64%). Of the 6-priority controlled PAEs in leachate, di-n-butyl phthalate was the most abundant (1046 µg L-1), while butyl phthalate was not detected. The results showed that pH, the relative abundance of Chloroflexi, and the value of SUVA254 can directly influence the occurrence of PAEs in leachate. The positive and negative effects vary depending on the PAE content and molecular weight. DBP and DEHP have higher environmental risks in the aquatic system. These results are intended to provide a scientific basis for the evolutionary characterization of the microenvironment in complex environmental systems and the control of novel contaminants, such as PAEs.

Full life cycle and sustainability transitions of phthalates in landfill: A review.

Phthalates (PAEs) are added to various products as a plasticizer. As these products age and are disposed of, plastic waste containing PAEs enters the landfill. The landfill environment is complicated and can be regarded as a "black box". Also, PAEs do not bind with the polymer matrix. Therefore, when a series of physical chemistry and biological reactions occur during the stabilization of landfills, PAEs leach from waste and migrate to the surrounding environmental media, thereby contaminating the surrounding soil, water ecosystems, and atmosphere. Although research on PAEs has achieved progress over the years, they are mainly concentrated on a particular aspect of PAEs in the landfill; there are fewer inquiries on the life cycle of PAEs. In this study, we review the presence of PAEs in the landfill in the following aspects: (1) the main source of PAEs in landfills; (2) the impact of the landfill environment on PAE migration and conversion; (3) distribution and transmedia migration of PAEs in aquatic ecosystems, soils, and atmosphere; and (4) PAE management and control in the landfill and future research direction. The purpose is to track the life cycle of PAEs in landfills, provide scientific basis for in-depth understanding of the migration and transformation of PAEs and environmental pollution control in landfills, and new ideas for the sustainable utilization of landfills.