Biodegradation of cefalexin by two bacteria strains from sewage sludge.

Bioremediation has been used as an environmentally-friendly, energy-saving and efficient method for removing pollutants. However, there have been very few studies focusing on the specific antibiotic-degrading microorganisms in the activated sludge and their degradation mechanism. Two strains of cefalexin-degrading bacteria (Rhizobium sp. (CLX-2) and Klebsiella sp. (CLX-3)) were isolated from the activated sludge in this study. They were capable of rapidly eliminating over 99% of cefalexin at an initial concentration of 10 mg l-1 within 12 h. The exponential phase of cefalexin degradation happened a little earlier than that of bacterial growth. The first-order kinetic model could elucidate the biodegradation process of cefalexin. The optimized environmental temperature and pH values for rapid biodegradation by these two strains were found to be 30°C and 6.5-7, respectively. Furthermore, two major biodegradation metabolites of CLX-3, 7-amino-3-cephem-4-carboxylic acid and 2-hydroxy-3-phenyl pyrazine were identified using UHPLC-MS and the biodegradation pathway of cefalexin was proposed. Overall, the results showed that Rhizobium sp. (CLX-2) and Klebsiella sp. (CLX-3) could possibly be useful resources for antibiotic pollution remediation.

Ecological risk assessment of current-use pesticides in an aquatic system of Shanghai, China.

The widespread use of current-use pesticides (CUPs) in modern agriculture has threatened the survival of aquatic organisms. Therefore, the residual levels, spatial distribution, and ecological risk assessment of 18 CUPs are investigated in an aquatic system of Shanghai. The aquatic system focused on a freshwater system that contains particles smaller than 0.45 µm in size, which are easily absorbed by aquatic organisms. The mean values of chlorpyrifos, napropamide, and atrazine were found to be the highest concentration CUPs, and propazine, mevinphos, ametryn, butylate, dichlorvos, ethoprop, and prometryn displayed the most significant positive correlations with each other. The concentration of the ∑18CUPs was higher in the southern areas of Shanghai (generally greater than 100 ng/L), but it was relatively low in the central areas (generally smaller than 75 ng/L). Six important CUPs were identified, and the differences in the concentration contribution rates and contribution amounts among different intensive land-use types were noticeable. The ecological risk in most areas of this aquatic system of Shanghai was high. Chlorpyrifos and butachlor produced the maximum toxic unit (mTU) for daphnid and green algae, respectively, and their toxic unit contribution rates to the entire mixture toxicity were both greater than 50%. This confirms that the mixture toxicity of the CUPs to aquatic organisms in this aquatic system of Shanghai primarily resulted from a few dominant toxic pesticides. However, for each sensitive organism, there will still be a risk contribution of approximately 5%-30% due to other CUPs.

Strategies of Phytophthora infestans adaptation to local UV radiation conditions.

Expected global changes in environmental conditions underline the need for a better understanding of genetic variation in ecological traits and their strategies of adaptation to the stresses. In this study, evolutionary mechanisms and processes of UV adaptation in plant pathogens were investigated by combining statistical genetics, physiological assays, and common garden experiment approaches in an assessment of the potato late blight pathogen, Phytophthora infestans, sampled from various geographic locations in China. We found spatial divergence caused by diversifying selection in UV tolerance among P. infestans populations. Local UV radiation was the driving force of selection as indicated by a positive correlation between UV tolerance in P. infestans populations and the altitude of collection sites. Plasticity accounted for 68% of population variation while heritability was negligible, suggesting temporary changes in gene expression and/or enzymatic activity play a more important role than permanent modification of gene structure in the evolution of UV adaptation. This adaptation strategy may explain the lack of fitness penalty observed in genotypes with higher UV tolerance.