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1.
Med Parazitol (Mosk) ; (3): 44-5, 2011.
Artículo en Ruso | MEDLINE | ID: mdl-21932546

RESUMEN

The efficacy of a kit of Opisthorchiasis-CIC-EIA-Best reagents was evaluated using 270 sera from patients in the study and control groups. The kit showed a sufficient sensitivity (not less than 87.2%) and a high specificity (not less than 97.9%). The use of the above kit of the reagents for enzyme immunoassay in practical healthcare enables one to increase detection rates among the infested subjects on comprehensive examination of those with suspected opisthorchiasis.


Asunto(s)
Anticuerpos Antihelmínticos/inmunología , Complejo Antígeno-Anticuerpo , Antígenos Helmínticos/inmunología , Técnicas para Inmunoenzimas , Opistorquiasis , Opisthorchis/crecimiento & desarrollo , Animales , Complejo Antígeno-Anticuerpo/inmunología , Estudios de Casos y Controles , Heces/parasitología , Humanos , Opistorquiasis/sangre , Opistorquiasis/diagnóstico , Opistorquiasis/inmunología , Opistorquiasis/parasitología , Recuento de Huevos de Parásitos , Federación de Rusia , Sensibilidad y Especificidad
2.
Khirurgiia (Mosk) ; (1): 25-9, 2010.
Artículo en Ruso | MEDLINE | ID: mdl-20336041

RESUMEN

Results of the immune-enzyme assay (IEA) of children with liver and lung echinococcosis before and after the operation were analyzed. The IEA accuracy was 91% for liver echinococcosis and 57% for lung echinococcosis. Sensitivity was 86% and 55, respectively. IEA was a reliable method of disease recurrence detection postoperatively. Conservative treatment of hydatid disease consisted of Antiparasitic and auxiliary therapy and was successfully applied in 7 patients with liver echinococcosis and 3 kids with combined affection. Preventive chemotherapy was carried out in 115 patients. During the 5 years follow-up period no recurrences was registered.


Asunto(s)
Anticestodos/uso terapéutico , Equinococosis Hepática/terapia , Equinococosis Pulmonar/terapia , Laparoscopía/métodos , Cirugía Torácica Asistida por Video/métodos , Adolescente , Animales , Anticuerpos Antihelmínticos/análisis , Niño , Preescolar , Equinococosis Hepática/diagnóstico , Equinococosis Pulmonar/diagnóstico , Echinococcus/inmunología , Echinococcus/aislamiento & purificación , Femenino , Estudios de Seguimiento , Humanos , Técnicas para Inmunoenzimas , Masculino , Estudios Retrospectivos , Tomografía Computarizada por Rayos X , Resultado del Tratamiento
3.
Med Parazitol (Mosk) ; (4): 22-6, 2005.
Artículo en Ruso | MEDLINE | ID: mdl-16445233
5.
Med Parazitol (Mosk) ; (1): 20-4, 2000.
Artículo en Ruso | MEDLINE | ID: mdl-10808712

RESUMEN

The major proteins of T. gondii excretory and secretory antigens (ESA) obtained during cultivation of tachyzoites by using cultured Vero cells were shown to have molecular weights of 79, 70, 57, 48, 36, and 29 kD. ESA and somatic antigen immunoblotting demonstrated that there were noticeable differences in the immunoactive proteins of these antigens. The antibodies of the sera of patients with toxoplasmosis mainly interacted with ESA proteins having molecular weights of 79, 70, 57, 48, 36, and 29 kD, 57-kD ESA protein antibodies being present on the immunoblots of all the tested sera. When ESA was used as an antigen during enzyme immunoassay, it showed a high sensitivity in the detection of IgM antigens in congenital toxoplasmosis. At the same time, ESA identified the antibodies of this class in the sera of healthy donors much infrequently than somatic antigen (p < 0.05).


Asunto(s)
Antígenos de Protozoos/análisis , Antígenos de Protozoos/inmunología , Estadios del Ciclo de Vida , Proteínas Protozoarias/análisis , Proteínas Protozoarias/inmunología , Toxoplasma/crecimiento & desarrollo , Toxoplasma/inmunología , Animales , Complejo Antígeno-Anticuerpo/sangre , Antígenos de Protozoos/clasificación , Estudios de Casos y Controles , Chlorocebus aethiops , Electroforesis en Gel de Poliacrilamida , Ensayo de Inmunoadsorción Enzimática , Humanos , Inmunoglobulina G/inmunología , Peso Molecular , Proteínas Protozoarias/clasificación , Sensibilidad y Especificidad , Pruebas Serológicas , Toxoplasmosis/inmunología , Toxoplasmosis Congénita/inmunología , Células Vero
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