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1.
Environ Geochem Health ; 46(10): 412, 2024 Sep 04.
Artículo en Inglés | MEDLINE | ID: mdl-39230594

RESUMEN

This paper presents findings on groundwater physiochemical composition and radioactivity levels in households in Bac Lieu province, Vietnam. Through discriminant analysis, it was observed that groundwater quality exhibits spatial variations corresponding to saline intrusion zones. The paired-samples T-tests revealed significantly different ratios of Ra-224, Ra-226, and Ra-228 isotopes between Na-Cl and Ca-Na-HCO3 water types. All three water types had a ratio of Ra-226/Ra-228 of approximately one, indicating the presence of groundwater aquifers beneath the crust and fluvial marine sediment. Furthermore, strong associations between sulfate and calcium suggest that CO2 enrichment in groundwater aquifers indicates anoxic aquatic environments. Twenty-five of the thirty-three evaluated samples exceeded the national technical regulations for domestic water quality with parameters such as chloride, sulfate, sodium, gross alpha, or total dissolved solids. Fifteen samples exceeded gross alpha's allowable contamination threshold of 0.1 Bq/L. The combination of Ra-226 and Ra-228 did not surpass the U.S. Environmental Protection Agency's recommended limit of 0.185 Bq/L. However, nineteen samples exhibited annual committed effective doses of radium isotopes for infants that exceeded the WHO recommendation of 0.1 mSv/year.


Asunto(s)
Agua Subterránea , Radio (Elemento) , Contaminantes Radiactivos del Agua , Vietnam , Agua Subterránea/química , Contaminantes Radiactivos del Agua/análisis , Humanos , Radio (Elemento)/análisis , Monitoreo de Radiación/métodos , Composición Familiar , Sulfatos/análisis
2.
One Health ; 18: 100685, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-39010965

RESUMEN

Transmission of extended-spectrum ß-lactamase (ESBL) genes has increased the global prevalence of ESBL-producing bacteria, especially in developing countries. Human infection with these bacteria may be food-mediated but has not been fully elucidated. Therefore, we aimed to examine ESBL-producing bacteria in edible river fish and elucidate their potential for horizontal gene transfer. A total of 173 ESBL-producing Enterobacterales were isolated (Escherichia coli [n = 87], Klebsiella pneumoniae [n = 52], Enterobacter cloacae complex [n = 18], Citrobacter freundii complex [n = 14], Atlantibacter hermannii [n = 1] and Serratia fonticola [n = 1]) from 56 of 80 fish intestinal contents sampled. Among the bacterial bla CTX-M genotypes, bla CTX-M-55 was the most predominant, followed by bla CTX-M-15, bla CTX-M-27, and bla CTX-M-65. Furthermore, we found that ESBL-producing Enterobacterales were able to transfer their bla CTX-M genes to E. coli. In summary, our results suggest that ESBL-producing Enterobacterales transfer bla CTX-M to indigenous gut E. coli in humans, following the consumption of contaminated fish.

3.
Microbiol Resour Announc ; 13(6): e0005624, 2024 Jun 11.
Artículo en Inglés | MEDLINE | ID: mdl-38682768

RESUMEN

Extended-spectrum ß-lactamase-producing Atlantibacter hermannii was isolated from an edible river fish, Anabas testudineus, which was sold in a market located in Vietnam. The genome sequence was obtained by using next-generation sequencing, which involved Oxford Nanopore and Illumina technologies. The 92 kb plasmid encodes the gene blaCTX-M-27.

4.
Mar Pollut Bull ; 198: 115812, 2024 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-38043208

RESUMEN

The spread of antibiotic-resistant bacteria is a global problem that should be addressed through the perspective of the "one health" concept. The purpose of this study was to determine the contamination rate of antibiotic-resistant Aeromonas spp. in fresh water river fish purchased from a fish market in Vietnam. We then defined the pattern of antibiotic resistance to assess antibiotic-resistant contamination. Antibiotic-resistant Aeromonas spp. were detected in the intestinal contents of 32 of 80 fish. blaNDM-1 was detected in seven strains. Extended-spectrum ß-lactamase and AmpC ß-lactamase-related genes were detected in 28 strains, including blaCTX-M-55, blaCTX-M-15, blaCTX-M-1, and blaDHA,blaFOX, and blaMOX. The blaNDM-1 detected in the seven Aeromonas spp. strains were found chromosomally. This finding suggests that the blaNDM gene is stable in the natural environment and may spread widely into animals and humans via Aeromonas spp. with a transposon. Our results suggest the importance of continuing to monitor carbapenemase genes in Aeromonas spp. to evaluate the possibility that they may spread in other Enterobacterales, and to elucidate the mechanism of spread.


Asunto(s)
Aeromonas , Humanos , Animales , Aeromonas/genética , Contenido Digestivo , Vietnam , beta-Lactamasas/genética , Proteínas Bacterianas/genética , Antibacterianos/farmacología , Peces/genética , Agua Dulce , Cromosomas , Pruebas de Sensibilidad Microbiana
5.
Microbiol Resour Announc ; 12(7): e0014923, 2023 Jul 18.
Artículo en Inglés | MEDLINE | ID: mdl-37367473

RESUMEN

Salmonella enterica SE20-C72-2 and Escherichia coli EC20-C72-1 were isolated from the edible fish Anabas testudineus in Vietnam. The chromosomes and plasmids from both strains were sequenced using Oxford Nanopore and Illumina sequencing. Plasmids approximately 250 kbp long, encoding blaCTX-M-55 and mcr-1.1, were detected in both strains.

6.
Microbiol Resour Announc ; 12(5): e0134422, 2023 May 17.
Artículo en Inglés | MEDLINE | ID: mdl-37010426

RESUMEN

Carbapenem-resistant Citrobacter freundii CF20-4P-1 and Escherichia coli EC20-4B-2 were isolated from edible Mastacembelidae in Vietnam. We present the draft genome sequences, and the complete plasmid genome sequencing was also performed by hybrid assembly sequencing of Oxford Nanopore and Illumina. The 137-kbp plasmid encoding the assembled blaNDM-1 was detected in both strains.

7.
Mar Pollut Bull ; 184: 114108, 2022 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-36166861

RESUMEN

The threat of antimicrobial resistance is increasing. Microbial food contamination poses a serious public health risk; however, there are only a few studies on the prevalence of colistin-resistant Escherichia coli (COL-E) contamination in freshwater fish. This study aimed to characterise the antibiotic resistance genes and antibiotic susceptibility profiles of COL-E in freshwater fish in Vietnam. In total, 103 fish were collected and 63 COL-E were isolated. COL-E was investigated by genotyping mcr and AmpC/extended-spectrum ß-lactamase (ESBL)-related genes. The results show that COL-E and AmpC/ESBL-producing COL-E were confirmed in 24.3 % and 14.6 % of the fish, respectively. Multiplex PCR for mcr-1-9 showed that all 63 COL-E harboured mcr-1, while mcr-3 was detected in 7.9 % of COL-E. The minimum inhibitory concentration of colistin ranged from 2 to 256 µg/mL. Meanwhile, antibiotic susceptibility results show that all COL-E were resistant to ampicillin, streptomycin, and chloramphenicol.


Asunto(s)
Infecciones por Escherichia coli , Proteínas de Escherichia coli , Animales , Colistina/farmacología , Escherichia coli , Proteínas de Escherichia coli/genética , beta-Lactamasas/genética , Farmacorresistencia Bacteriana/genética , Plásmidos , Pruebas de Sensibilidad Microbiana , Antibacterianos/farmacología , Agua Dulce , Ampicilina , Estreptomicina , Cloranfenicol/análisis
8.
Arch Microbiol ; 204(2): 137, 2022 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-35032196

RESUMEN

Although the spread of plasmid-mediated antibiotic-resistant bacteria is a public health concern, food contamination with plasmid-mediated antibiotic-resistant Escherichia coli in Vietnam has not been well investigated. This study aimed to describe the prevalence of colistin-resistant, carbapenem-resistant, and endemic blaCTX-M in extended-spectrum ß-lactamase (ESBL) producing E. coli isolates. Colistin and carbapenem-resistant ESBL-producing E. coli were isolated from chickens in Vietnam and Japan. Colistin-resistant and AmpC/ESBL-producing E. coli (52% and 93%, respectively) were detected in chickens from Vietnam, in comparison to 52.7%, AmpC/ESBL-producing E. coli found in chicken from Japan. Carbapenem-resistant E. coli has not been isolated in Vietnam and Japan. Genotyping revealed that colistin-resistant E. coli harboured mcr-1, and most of the AmpC/ESBL-related genes were blaCTX-M-55 and blaCTX-M-65 together with blaTEM in Vietnamese chickens and blaCMY-2 in Japanese chickens. Multi-drug resistance analysis showed that ESBL-producing E. coli isolates had greater resistance to quinolones, streptomycin, and chloramphenicol than colistin-resistant E. coli isolates from Vietnam, suggesting the selection of multiple antibiotic resistance genes in ESBL-producing E. coli. In conclusion, colistin-resistant E. coli was detected in approximately half of the chicken samples, the majority of which harboured mcr-1. The high prevalence of ESBL-producing E. coli has remained constant in the last 5 years. The predominant blaCTX-M in ESBL-producing E. coli was blaCTX-M-55 or blaCTX-M-65, with the coexistence of blaTEM in Vietnam. These results can be implemented in monitoring systems to overcome the development of antimicrobial resistance.


Asunto(s)
Infecciones por Escherichia coli , Proteínas de Escherichia coli , Animales , Antibacterianos/farmacología , Pollos , Colistina/farmacología , Escherichia coli/genética , Infecciones por Escherichia coli/veterinaria , Proteínas de Escherichia coli/genética , Carne , Plásmidos/genética , Vietnam , beta-Lactamasas/genética
9.
Sci Total Environ ; 737: 140291, 2020 Oct 01.
Artículo en Inglés | MEDLINE | ID: mdl-32783865

RESUMEN

This paper presents information on the concentration of radioactivity and chemicals at 12 water stations that used groundwater as their raw water supply source. The groundwater's radioactivity was higher than the treated, tap, and surface water, but lower than the national and international recommendations. At five stations (41.7%), the gross alpha contents were higher than the levels advised by Vietnam's regulations, but met the WHO and IAEA's recommendations. The mean activity (Bq L-1) gross alpha, gross beta, Ra-224, Ra-226, and Ra-228 were 0.093 ± 0.012, 0.221 ± 0.020, 0.031 ± 0.004, 0.028 ± 0.004, and 0.035 ± 0.001, respectively. The contribution of Ra-226 to the gross alpha was in a range of 23%-60% (r = 0.91, p value <0.001), and the ratio of Ra-226/Ra-228 ranged from 0.49-1.06. For the treated and tap water, each age groups' annual committed effective dose was lower than the international regulations. The concentration of the total dissolved solids, sulfate, chloride, sodium, barium, and manganese met the national regulations. However, during the rainy season, the surface water in the area was affected by saltwater intrusion, with salinity up to 4.1‰. Discriminant analysis was applied to study the differences among the water groups. As a result, the treated and tap water were separated from the others.


Asunto(s)
Monitoreo de Radiación , Radiactividad , Contaminantes Radiactivos del Agua/análisis , Salud Pública , Vietnam , Abastecimiento de Agua
10.
Asia Pac J Public Health ; 32(2-3): 126-132, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32174126

RESUMEN

Antibiotic residues in chicken meat samples is a serious public health concern due to their harmful effects on consumers' health. We conducted this research in 2017 to determine antibiotic residues in chicken meat samples and to describe the knowledge, attitude, and practice (KAP) of farmers on the antibiotic uses in poultry farms at Tay Ninh province. Farmers who raised chickens at their farmhouses were surveyed using a KAP survey questionnaire, and 106 chicken meat samples were analyzed for 29 kinds of antibiotic residues. The results showed that 27.4% samples were positive with 11 kinds of 4 antibiotic groups: fluoroquinolones, sulfonamides, tetracycline, and macrolides. A total of 50.9% farmers needed to improve their knowledge on the appropriate use of antibiotics. Although 54.7% famers had positive attitude, just 17% had good practices. It was recommended that the levels of antibiotic residues in chickens should be communicated to food safety and veterinary authorities, chicken farmers, and the general public. Enhancing the training and coaching for each group of farmers to improve their knowledge and practices on the appropriate use of antibiotics in raising chickens is needed. Local government should enhance the level of control and punishment for drug sellers and end users regarding the use of banned antibiotics in chickens.


Asunto(s)
Antibacterianos/uso terapéutico , Residuos de Medicamentos/análisis , Agricultores/psicología , Conocimientos, Actitudes y Práctica en Salud , Carne/análisis , Animales , Pollos , Estudios Transversales , Inocuidad de los Alimentos , Humanos , Encuestas y Cuestionarios , Vietnam
11.
Parasitol Int ; 66(4): 503-509, 2017 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-27746382

RESUMEN

Opisthorchiasis caused by Opisthorchis viverrini is an important foodborne trematodiasis in Thailand, Laos and Cambodia. Interestingly, the opisthorchiasis endemic region overlaps with an area of leptospirosis emergence. Here we report an association between opisthorchiasis and leptospirosis in Thailand. Of 280 sera collected from villagers living around the Lawa wetland complex in Khon Kaen province, 199 (71%) were seropositive for leptospirosis by immunochromatography. Individuals with O. viverrini infection had a significantly higher rate of leptospirosis than those without (P=0.001). Significant higher leptospirosis prevalence was found in males than females (P=0.002). However, females but not males with O. viverrini infection showed a significantly higher seroprevalence of leptospirosis. Twenty-one of 35 environmental samples from the lake (water, mud and fish skin mucus) were positive for Leptospira spp. DNA sequencing, sequence alignment, and phylogenetic analysis of some positive nested PCR products revealed both pathogenic and intermediate pathogenic strains of Leptospira in the samples. Strikingly, O. viverrini metacercariae from the fish were positive for L. interrogans. These results suggest a close association between opisthorchiasis and leptospirosis. Contact with water, mud or eating raw fish harboring liver fluke metacercariae may be risk factors for Leptospira infection.


Asunto(s)
Enfermedades Endémicas , Leptospira/fisiología , Leptospirosis/epidemiología , Opistorquiasis/epidemiología , Opisthorchis/fisiología , Adulto , Animales , Proteínas Bacterianas/análisis , Femenino , Humanos , Leptospira/genética , Leptospira/aislamiento & purificación , Leptospirosis/microbiología , Masculino , Persona de Mediana Edad , Opistorquiasis/parasitología , Opisthorchis/aislamiento & purificación , Filogenia , Prevalencia , Análisis de Secuencia de ADN , Estudios Seroepidemiológicos , Tailandia/epidemiología
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