RESUMEN
Controlling the spatial distribution of catalytic sites in metallo-folded single-chain nanoparticles (SCNPs) is a first step toward the rational design of improved catalytic soft nano-objects. Here an unexplored pathway is reported for tuning the internal structure of metallo-folded SCNPs. Unlike the conventional SCNP synthesis in good solvent (protocol I), the proposed new route (protocol II) is based on the use of amphiphilic random copolymers and transfer, after SCNP formation, from selective to good (nonselective) solvent conditions. The size and morphology of the SCNPs obtained by the two protocols, and the corresponding spatial distribution of the catalytic sites, have been determined by combining results from size exclusion chromatography with triple detection, small-angle X-ray scattering and molecular dynamics (MD) simulations. Remarkably, the use of these protocols allows the tuning of the internal structure of the metallo-folded SCNPs, as supported by MD simulations results. While the conventional protocol I yields a homogeneous distribution of the catalytic sites in the SCNP, these are arranged into clusters in the case of protocol II.
Asunto(s)
Nanopartículas del Metal/química , Compuestos Organometálicos/química , Polímeros/química , Simulación de Dinámica Molecular , Estructura Molecular , Compuestos Organometálicos/síntesis química , Tamaño de la Partícula , Polímeros/síntesis química , Solventes/químicaRESUMEN
Inspired by the multifunctionality of vitamin D-binding protein and the multiple transient-binding behavior of some intrinsically disordered proteins (IDPs), a polymeric platform is designed, prepared, and characterized for combined delivery of dermal protective and anticancer bioactive cargos on the basis of artificial single-chain nano-objects mimicking IDPs. For the first time ever, simultaneous delivery of folic acid or vitamin B9 , and hinokitiol, a relevant natural bioactive compound that exhibits anticancer activity against human malignant melanoma cells, from these multidirectionally self-assembled unimolecular nanocarriers is illustrated.
Asunto(s)
Portadores de Fármacos/química , Proteínas Intrínsecamente Desordenadas/química , Nanopartículas/química , Antineoplásicos/administración & dosificación , Línea Celular Tumoral , Portadores de Fármacos/síntesis química , Ácido Fólico/administración & dosificación , Ácido Fólico/farmacología , Humanos , Proteínas Intrínsecamente Desordenadas/síntesis química , Melanoma/tratamiento farmacológico , Melanoma/patología , Simulación de Dinámica Molecular , Imitación Molecular , Monoterpenos/administración & dosificación , Sustancias Protectoras/administración & dosificación , Sustancias Protectoras/farmacología , Piel/efectos de los fármacos , Tropolona/administración & dosificación , Tropolona/análogos & derivadosRESUMEN
Conformational properties of star-shaped polymer aggregates that carry attractive end groups, called telechelic star polymers, are investigated by simulation and analytical variational theory. We focus on the case of low telechelic star polymer functionalities, f < or = 5, a condition which allows aggregation of all attractive monomers on one site. We establish the functionality- and polymerization-number dependence of the transition temperature from the "star burst" to the "watermelon" macroparticle structure. Extensions to telechelic stars featuring partially collapsed configurations are also discussed.