Nitrate denitrification rate response to temperature gradient change during river bank infiltration.

Nitrate attenuation during river bank infiltration is the key process for reducing nitrogen pollution. Temperature is considered to be an important factor affecting nitrate attenuation. However, the magnitude and mechanism of its impact have not been clear for a long time. In this study, the effects of temperature and temperature gradient on the nitrate denitrification rate were investigated via static batch and dynamic soil column simulation experiments. The results showed that temperature had a significant effect on the denitrification rate. Temperature effects were first observed in denitrifying bacteria. At low temperatures, the microorganism diversity was low, resulting in a lower denitrification rate constant. The static experimental results showed that the denitrification rate at 19 °C was approximately 2.4 times that at 10 °C. The dynamic soil column experiment established an exponential positive correlation between the nitrate denitrification decay kinetic constant and temperature. The affinity of denitrifying enzymes for nitrate in the reaction substrate was ordered as follows: decreasing temperature gradient (30 °C → 10 °C) > zero temperature gradient (10 °C) > increasing temperature gradient condition (0 °C → 10 °C). This study provides a theoretical basis for the biogeochemical processes underlying river bank infiltration, which will help aid in the development and utilization of groundwater resources.

Driving mechanism of different nutrient conditions on microbial mediated nitrate reduction in magnetite-present river infiltration zone.

Significant research is focused on the ability of riparian zones to reduce groundwater nitrate contamination. Owing to the extremely high redox activity of nitrate, naturally existing electron donors, such as organic matter and iron minerals, are crucial in facilitating nitrate reduction in the riparian zone. Here, we examined the coexistence of magnetite, an iron mineral, and nitrate, a frequently observed coexisting system in sediments, to investigate nitrate reduction features at various C/N ratios and evaluate the response of microbial communities to these settings. Additionally, we aimed to use this information as a foundation for examining the effect of nutritional conditions on the nitrate reduction process in magnetite-present environments. These results emphasise the significance of organic matter in enabling dissimilatory nitrate reduction to ammonium (DNRA) and enhancing the connection between nitrate reduction and iron in sedimentary environments. In the later phases of nitrate reduction, nitrogen fixation was the prevailing process in low-carbon environments, whereas high-carbon environments tended to facilitate the breakdown of organic nitrogen. High-throughput sequencing analysis revealed a robust association between C/N ratios and alterations in microbial community composition, providing insights into notable modifications in essential functioning microorganisms. The nitrogen-fixing bacterium Ralstonia is more abundant in ecosystems with scarce organic matter. In contrast, in settings rich in organic matter, microorganisms, such as Acinetobacter and Clostridia, which may produce ammonia, play crucial roles. Moreover, the population of iron bacteria grows in such an environment. Hence, this study proposes that C/N ratios can influence Fe(II)/Fe(III) conversions and simultaneously affect the process of nitrate reduction by shaping the composition of specific microbial communities.

Quantifying the effect of the nitrogen biogeochemical processes on the distribution of ammonium in the riverbank filtration system.

Ammonium (NH4+) enrichment of riverbank filtration (RBF) systems is gaining popularity. However, most previous research has concentrated on NO3- removal efficiencies, while the mechanisms of NH4+ enrichment remain unknown. A nitrogen biogeochemical process model was developed for the quantitative analysis of NH4+ enrichment in the Kaladian well field in northwest Songyuan City, NE China. Data from laboratory experiments and in-situ monitoring were used to determine initial values and calibrate the thermodynamic/kinetic parameters representing nitrogen (N) biogeochemical reactions. (1) The NO3- from river was subjected to denitrification (DNF) and dissimilatory nitrate reduction to ammonium (DNRA) within 10-14 m of the shore, whereas the NH4+ in groundwater was caused by DNRA, organic nitrogen mineralization (MIN), and mixing with laterally recharged high NH4+ groundwater. (2) DNF and DNRA were regulated by hydrodynamic processes, with the ranges of these processes being more significant in the wet season due to a higher hydraulic gradient. MIN occurred widely throughout the water flow path, with temperature primarily controlling the rates of the three reactions. (3) DNRA activity was relatively higher in the wet season when the water temperature was higher within 10-14 m of the shore. In the wet season, DNRA contributed 25%-30% to NO3- reduction, which was higher than in the dry season (5%-10%). DNRA contributed at least 40% and 15% to NH4+ enrichment in the wet and dry seasons, respectively. (4). Organic N in media gradually released NH4+ into groundwater via MIN and desorption across the entire flow path, with contributions to NH4+ enrichment reaching 75% and 85%, respectively, in the wet and dry seasons.

Effects of magnetite on microbially driven nitrate reduction processes in groundwater.

Nitrate is a common pollutant in the aquatic environment. Denitrification and dissimilatory nitrate reduction to ammonium (DNRA) are the main reduction processes of nitrate. In the relatively closed sediment environment, the competitive interaction of these two nitrate reduction determines whether the ecosystem removes or retains nitrogen. In the process of NO3--N bioreduction, Magnetite, which is a common mineral present in soil and other sediments can play a crucial role. However, it is still not clear whether magnetite promotes or inhibits NO3--N bioreduction. In this paper, the effect of magnetite on NO3--N bioreduction was studied by batch experiments. The results show that magnetite can increase the NO3--N reduction rate by 1.48 %, and can inhibit the DNRA process at the beginning of the reaction and then promote the DNRA process. Magnetite changed the microbial community structure in our experiment systems. The relative abundance of Sphingomonas, which mainly exists in a high carbon and low nitrogen environment, increased under sufficient carbon source conditions. The relative abundance of Fe-oxidizing and NO3--N reducing bacteria, such as Flavobacterium, increased in the absence of carbon sources but in the presence of magnetite. In addition, magnetite can significantly increase activity of the microbial electron transport system (ETS). the added microbial electronic activity of magnetite increased nearly two-fold under the same experiment conditions. The acid produced by the metabolisms of Pseudomonas and Acinetobacter further promotes the dissolution of magnetite, thus increasing the concentration of Fe (II) in the system, which is beneficial to autotrophic denitrifying bacteria and promote the reduction of NO3--N. These findings can enhance our understanding of the interaction mechanism between iron minerals and nitrate reducing bacteria during nitrate reduction under natural conditions.

Effects of carbon load on nitrate reduction during riverbank filtration: Field monitoring and batch experiment.

Riverbank filtration (RBF) is a well-established technique worldwide, and is critical for the maintenance of groundwater quality and production of clean drinking water. Evaluation of the decay of exogenous nitrate (NO3-) in river water and the enrichment of ammonium (NH4+) in groundwater during RBF is important; these two processes are mainly influenced by denitrification (DNF) and dissimilatory nitrate reduction to ammonium (DNRA) controlled by the groundwater carbon load. In this study, the effects of carbon load (organic carbon [OC]: NO3-) on the competing nitrate reduction (DNRA and DNF) were assessed during RBF using field monitoring and a laboratory batch experiment. Results show the groundwater OC: NO3- ratio did not directly affect the reaction rate of DNRA and DNF, however, it could control the competitive partitioning between the two. In the near-shore zone, the groundwater OC: NO3- ratio shows significant seasonal variations along the filtration path owing to the changing conditions of redox, OC-rich, and NO3--limited. A greater proportion of NO3- would be available for DNRA in the wet season with higher OC: NO3- ratio (> 10), resulting in a significantly NH4+-N enrichment rate (from 1.43 × 10-3 to 9.54 × 10-4 mmol L-1 d-1) in the near-shore zone where the zone of Mn (IV) oxide reduction. However, the activity of DNRA was suppressed with lower OC: NO3- ratio (< 10) in the dry season, resulting in a stable NH4+-N enrichment rate (from 3.12 × 10-4 to 1.30 × 10-4 mmol L-1 d-1). Benefiting from seasonal variation of OC-rich and NO3--limited conditions, DNRA bacteria outcompeted denitrifiers, which eventually led to seasonal differences in NO3- reduction in the near-shore zone. Overall, under the effect of DNRA induced by continuous high carbon load in RBF systems, nitrogen input is not permanently removed but rather retained in groundwater during RBF.

Adsorption/desorption of naphthalene and phenanthrene in a binary competitive system in the riparian zone.

Polycyclic aromatic hydrocarbons (PAHs) such as naphthalene (Nap) and phenanthrene (Phe) are organic pollutants that are of concern because of their environmental toxicity. Adsorption is a promising process for the removal of Nap and Phe from water and soil. The riparian zone between a river and a riparian aquifer, which is rich in adsorption medium, may be important for PAH remediation. Nap and Phe may be removed from the surface water through adsorption by the media in the riparian zone. However, there is still a lack of the removal patterns and mechanisms of media in the riparian zone to remediate water contaminated by Nap and Phe simultaneously. In this study, focusing on the typical PAHs (Nap and Phe) as target pollutants, batch static adsorption and desorption experiments of Nap and Phe were carried out to explore the competitive adsorption mechanisms of Nap and Phe in the binary system. Batch dynamic adsorption experiments were conducted to ascertain the adsorption regulation of Nap and Phe in sediments during the recharge of groundwater by river water in a riparian zone. The static adsorption experiment results showed that competitive adsorption of Nap and Phe occurred, and a mutual inhibitory effect of Nap and Phe adsorption was observed in the binary system. Phe had a stronger inhibitory effect on Nap, Phe was preferentially adsorbed on the medium in binary adsorption. The results of batch dynamic experiments showed that, in terms of adsorption, the riparian zone in the study area showed stronger performance for removal of Phe than Nap. The results of this paper could be useful for alleviating Nap and Phe pollution of groundwater and developing treatment protocols for groundwater exposed to Nap and Phe.

Influencing mechanisms of siderite and magnetite, on naphthalene biodegradation: Insights from degradability and mineral surface structure.

Biodegradation is the most economical and efficient process for remediating polycyclic aromatic hydrocarbons (PAHs) such as naphthalene (Nap). Soil composition is pivotal in controlling PAH migration and transformation. Iron minerals such as siderite and magnetite are the primary components of soil and sediment and play key roles in organic pollutant biodegradation. However, it is unclear whether siderite and magnetite promote or inhibit Nap biodegradation. The effects of siderite and magnetite on Nap biodegradation were investigated through batch experiments in this study. The results indicated that siderite increased Nap biodegradation efficiency by 7.87%, whereas magnetite inhibited Nap biodegradation efficiency by 3.54%. In the presence of siderite, Nap-degrading bacteria with acid-producing effects promoted siderite dissolution via metabolic activity, resulting in an increased Fe (II) concentration in solution which accelerated the iron reduction process and promoted Nap biodegradation. In addition, the presence of iron minerals altered the genus-level community structure. Anaerobic sulfate-reducing bacteria such as Desulfosporosinus occurred in the presence of siderite, indicating that sulfate reduction occurred in advance under the influence of siderite. In the presence of magnetite, Fe (III) in iron minerals were converted to Fe (II), and under the mediation of microorganisms, Fe (II) combined with carbonate to form secondary minerals (e.g., siderite). Secondary minerals were attached to the surface of magnetite, which inhibited magnetite dissolution and reduced the efficiency of Fe (III) utilization by microorganisms. Furthermore, as the reaction proceeds, acid-producing microorganisms promoted magnetite further dissolution, resulting in a longer duration of the Fe (III) reduction process. Bacteria utilizing sulfuric acid as the terminal electron acceptor consumed organic matter more rapidly than those using iron as the terminal electron acceptor. Therefore, magnetite inhibited Nap degradation. These observations enhance our understanding of the interaction mechanisms of iron minerals, organic pollutants, and degrading bacteria during the biodegradation process.

Experimental study on the biodegradation of naphthalene and phenanthrene by functional bacterial strains in the riparian soil of a binary system.

Polycyclic aromatic hydrocarbons (PAHs) such as naphthalene (Nap) and phenanthrene (Phe) are organic pollutants of concern owing to their toxicity, carcinogenicity, and teratogenicity. Biodegradation is considered the most economical and efficient process to remediate Nap and Phe. The riparian zone between a river and a riparian aquifer, which is rich in indigenous microorganisms, may be important for PAH remediation. However, few studies have evaluated the ability of indigenous microorganisms to remove Nap and Phe. In this study, focusing on the typical PAHs (Nap and Phe) as target pollutants, the genus-level community structure of Nap- and Phe-degrading bacteria was identified. Batch static and dynamic biodegradation experiments were conducted to explore the biodegradation mechanisms of Nap and Phe in the riparian zone and identify the factors influencing Nap and Phe biodegradation in the binary system (i.e., where Nap and Phe are simultaneously present). According to the genus-level community structure test results, the dominant bacterial genus in the binary system was mainly the Phe-degrading bacteria. The Nap and Phe-biodegradation percentages were 19.20% lower and 19.49% higher, respectively, in the binary system than in the unitary system. The results indicated that functional bacteria can degrade Nap and Phe, and that Nap weakly promoted Phe biodegradation. Additionally, the initial Nap and Phe concentration ratio, hydraulic gradient, and temperature affected Nap and Phe biodegradation. Dynamic biodegradation experiments showed that the biodegradation percentage decreased as the hydraulic gradient increased, and biodegradation percentage of Phe was always higher than that of Nap. According to the results of the dynamic laboratory experiments, the removal percentages of Nap and Phe by indigenous riparian-zone microorganisms were 6.21-16.73% and 13.95-24.45%, respectively. The findings in this study will be useful for alleviation of Nap and Phe pollution in groundwater and will facilitate determination of appropriate treatment measures for groundwater exposed to this type of pollution.

Mechanism of Suspended Kaolinite Particle Clogging in Porous Media During Managed Aquifer Recharge.

Managed aquifer recharge is an effective strategy for urban stormwater management. Chemical ions are normally retained in stormwater and groundwater and may accelerate clogging during the recharge process. However, the effect of water chemistry on physical clogging has not previously been investigated. In this study, we investigated the hydrogeochemical mechanism of saturated porous media clogging in a series of column experiments. The column was packed with river sand and added suspensions of kaolinite particles. Calcium chloride and sodium chloride are used as representative ions to study chemical effects. We found that an increase in ionic strength resulted in retention of kaolinite solids in the column, with a breakthrough peak of C/C0 value of 1 to 0.2. The corresponding hydraulic conductivity decreased with increased solids clogging. Divalent cations were also found to have a greater influence on kaolinite particle clogging than monovalent cations. The enhanced hydrochemical-related clogging was caused by kaolinite solids flocculating and increasing the deposition rate coefficient by 1 to 2 times in high ionic strength conditions. Three clogging mechanisms of kaolinite solids are proposed: surface filtration, inner blocking, and attachment. This study further deepens the understanding of the mechanisms of solids clogging during aquifer recharge and demonstrates the significance of ionic strength on recharge clogging risk assessments.

Natural attenuation of naphthalene along the river-bank infiltration zone of the Liao River, Shenyang, China.

In this study, the natural attenuation of naphthalene during riverbank infiltration was examined using batch experiments. The results indicated that, as the grain size and the permeability coefficient decreased, the natural attenuation rate of naphthalene increased, and it was highest in loam (62%) and lowest in coarse sand (20%). The half-life of naphthalene was longest in coarse sand (700 d) and shortest in mild clay (250 d). Facultative anaerobes such as Methylophilaceae accounted for about 70% of the total bacteria and played a major role in naphthalene degradation. A high total organic carbon concentration and large specific surface area can promote natural attenuation of naphthalene. Moreover, the adsorption to riverbank sediment in the hyporheic zone and bioremediation by indigenous microorganisms can effectively eliminate naphthalene during river water infiltration to the riverbank aquifer.