RESUMEN
To improve the accuracy of vibration velocity monitoring during blasting in soil layers, this paper provides a method and device for data correction by combining finite element software and actual engineering test data. Based on the length of the test pedestal exposed to the surface of the geotechnical body, the finite element structural model corresponding to each length of the test pedestal is established. Moreover, a predetermined external excitation load is applied outside the finite element model and the correction function of the vibration data is obtained by analysis of the stress and vibration data. The device solves the problem of low accuracy of vibration velocity measurement in soil and establishes a correction method for measurement data. The results show the following: (1) With the propagation of blasting seismic waves, the maximum stress values of the test device appear in the footwall position, the middle of the extension rod, and the bottom position in that order. (2) At the end of the test, there is an obvious phenomenon of speed amplification at the top of the test device. (3) As the length of the test device exposed to the ground increases, the particle peak vibration velocity (PPV) of the test device varies exponentially with the PPV of the ground and the range of variation of the vibration velocity in the X-direction is the largest.
RESUMEN
Dermal papilla cells (DPCs), an important component of hair follicles, its proliferation and apoptosis directly regulate and maintain the growth of hair follicles. All-trans-retinoic acid (ATRA) plays a critical role in hair growth. In this study, the effects of ATRA on cultured mink hair follicle growth were studied by administration of different concentrations of ATRA for 12 days in vitro. In addition, the proliferation and apoptosis of DPCs were measured after treating with ATRA. The mRNA and protein levels of hair follicle growth associated factor transforming growth factor-ß2 (TGF-ß2) and the phosphorylation levels of Smad2/3 were determined. Moreover, TGF-ß type I and type II receptor inhibitor LY2109761 and specific inhibitor of Smad3 (SIS3) were administered to investigate the underlying molecular mechanism. The results showed that ATRA inhibited hair follicle growth, promoted TGF-ß2 expression and activated phosphorylation of Smad2/3. In addition, ATRA inhibited cell proliferation by arresting the cell cycle at G1 phase and induced apoptosis of DPCs by enhancing the ratio of Bax/Bcl-2 and promoted the cleavage of caspase-3. Furthermore, LY2109761 or SIS3 partially reversed the decreased cell viability, increased apoptosis that were induced by ATRA. In conclusion, ATRA could inhibit hair follicle growth via inhibiting proliferation and inducing apoptosis of DPCs partially through the TGF-ß2/Smad2/3 pathway.