Hazardous Gases-Responsive Photonic Crystals Cryogenic Sensors Based on Antifreezing and Water Retention Hydrogels.

Nowadays, the sensing of hazardous gases is urgent for the consideration of public safety and human health, especially in extreme conditions of low temperatures. In this study, a photonic crystals (PhCs) sensor with water retention and antifreezing properties was developed and applied to visual hazardous gases sensing at low temperature, passively. The sensor was prepared by dip-coating with poly(methyl methacrylate) (PMMA) colloidal microspheres followed by embedding in k-carrageenan/polyacrylamide-ethylene glycol (k-CA/PAM-EG) hydrogel. The sensor responded to hazardous gases, including ammonia, toluene, xylene, acetone, methanol, ethanol, and 1-propanol, with a change in the reflection wavelength and visible structural color. At room temperature, the reflection wavelength of the sensor blue-shifted 49 nm in ammonia, and the structural color changed from red to yellow. For low temperatures, the sensor showed great water retention and antifreezing properties even at -57 °C due to the double network. The sensor still had a great response to hazardous gases after freezing at -20 °C for 12 h and testing at 0 °C, and the obtained results were similar to those at room temperature. Based on this excellent stability and visual sensing at low temperature, the sensor demonstrates the potential for detection of hazardous vapors in extreme environments.

A Terahertz Identification Method for Internal Interface Structures of Polymers Based on the Long Short-Term Memory Classification Network.

Polymers are used widely in the power system as insulating materials and are essential to the power grid's security and stability. However, various insulation defects may occur in the polymer., which can lead to severe insulation accidents. Terahertz (THz) detection is a novel non-destructive testing (NDT) method that is able to detect the interface structures inside polymers. The large quantity of information in the THz waveform has potential for the identification of interface types, and the long short-term memory (LSTM) network is one of the most popular artificial intelligence methods for time series data like THz waveform. In this paper, the LSTM classification network was used to identify the internal interfaces of the polymer with the reflected THz pulses of the internal interfaces. The experiment verified that it is feasible to identify and image the void interfaces and impurity interfaces in the polymer using the proposed method.

Reevaluation of adverse drug reactions of psychiatric drugs under the chinese drug volume-based procurement policy.

BACKGROUND: The "4 + 7" volume-based procurement is a "large group purchase" led by the Chinese government, with the aim of reducing the price of medicines by trading volume for price. Although the "4 + 7" drugs had passed the national consistency evaluation, the adverse drug reactions need to be further evaluated to ensure the safety of the "4 + 7" drugs with low prices. We aimed to analyze the occurrence characteristics and related influencing factors of adverse reactions of psychiatric drugs under the chinese drug volume-based procurement policy(4 + 7 policy), and provide references for clinical medication. METHODS: 137 cases of adverse drug reactions of four psychotropic drugs reported under the "4 + 7" policy in Wuxi Mental Health Center in 2020 were collected. The gender and age of patients, related "4 + 7" drugs, involving organs / systems, clinical manifestations, distribution of new / serious adverse reactions, clinic outcomes were analyzed. RESULTS: Among the 137 cases of adverse drug reactions, the incidence of adverse drug reactions was the highest in patients aged 61-70 (25.38%). Mainly involved 4 "4 + 7" psychiatric drugs, of which olanzapine tablets caused the most adverse reactions (54, 39.24%). The adverse reactions mainly involved the digestive system, nervous system, cardiovascular system, blood and lymphatic system, among which the digestive system was the most common (61, 44.53%). A total of 8 cases (6.16%) of new and 26 cases of serious adverse reactions were reported, all of which led to the prolongation of disease course. Except for the transient side effects, most of that were improved or cured with no death, disability or teratogenicity after stopping or reducing the dose with symptomatic treatment. CONCLUSION: Since more and more drugs will be included in "4 + 7" for clinic, clinical pharmacists should strengthen the publicity and training of the knowledge of "4 + 7" drugs, strengthen the monitoring of adverse drug reactions, and provide timely feedback to the clinic, in order to achieve early prevention, early identification, timely diagnosis and reasonable intervention of the adverse drug reactions under the context of "4 + 7" policy.

Early intervention of acute liver injury related to venlafaxine: A case report.

RATIONALE: Drug-induced liver injury (DILI) is the leading cause of acute liver injury (ALI), market withdrawal of a drug, and rejection of applications for marketing licenses. The incidence of DILI is very low, with a value between 1 and 19 per 100,000 patient years. All antidepressants may induce DILI even at low therapeutic doses. In this report, we present a case of ALI after venlafaxine administration. PATIENT CONCERNS: A 27-year-old Chinese Han woman was admitted for depression. Several serum liver function indices in this patient were abnormal after antidepressant treatment. The Roussel Uclaf Causality Assessment Method (RUCAM) causality assessment score was 8, and the R value was 31.18. DIAGNOSES: The patient was diagnosed with hepatocellular ALI, which was derived from venlafaxine-related adverse events. INTERVENTIONS: First, all medications were stopped to block the progression of DILI. Then, a hepatoprotective strategy and proper psychological treatment were performed to recover the impaired hepatic function. OUTCOMES: Liver function was fully recovered as indicated by liver function indices and ultrasound imaging. LESSONS: The possibility of DILI should not be overlooked during the long-term use of antipsychotic drugs. In response, regular liver function monitoring should be performed in a timely manner to avoid missing diagnoses and delayed treatment. Furthermore, the necessary medical treatment needs to be conducted after the occurrence of ALI.

CYP1A2 polymorphism may contribute to agomelatine-induced acute liver injury: Case report and review of the literature.

RATIONALE: Liver function monitoring is recommended when agomelatine is prescribed, although liver enzymes are not considered predictive biomarkers. Most patients present with acute liver injury, with only a few presenting with levels of liver enzymes that are over 30 times the upper limit of normal. The patient-specific risk factors that are associated with liver injury remain unclear. Thus, this report provides new insights into the mechanism of agomelatine-induced acute hepatocellular injury based on cytochrome P450 family 1 subfamily A member 2 (CYP1A2) polymorphism. PATIENT CONCERNS: We present a case of acute hepatocellular injury in a 75-year-old man who was taking agomelatine at a dose of 50 mg/qn. All hepatitis virus test results were negative. No history of liver disease was observed. As CYP1A2 is the main metabolic enzyme of agomelatine, CYP1A2 AA (rs762551) genetic polymorphism was analyzed. DIAGNOSIS: The patient's transaminases level exceeded the critical value on day 72 after starting oral agomelatine. INTERVENTIONS: The patient received intravenous magnesium isoglycyrrhizinate, a liver cell-protecting agent, followed by the withdrawal of agomelatine. OUTCOMES: There was an improvement in the levels of the liver enzymes and no subsequent organ dysfunction was observed. LESSONS: Here, we report a case of acute hepatocellular injury characterized by a very high aspartate aminotransferase level. Periodic liver function testing throughout the treatment period can help in the rapid and appropriate diagnosis of acute liver injury, particularly in the absence of typical clinical manifestations. Agomelatine hepatic toxicity might be related to an idiosyncratic metabolic reaction that depends on individual patient differences. As it is the main metabolic enzyme of agomelatine, CYP1A2 genetic polymorphism may contribute to liver injury by affecting its metabolites.

A novel and sensitive method for determination of amisulpride in human plasma by two-dimensional liquid chromatography.

A novel and sensitive heart-cutting two-dimensional liquid chromatography with ultraviolet detection method (2D-LC-UV) was developed and validated for determination of amisulpride in human plasma. The 2D-LC system consists of a first dimensional (1 D) LC column and a middle transfer column as well as a second-dimensional (2 D) LC column. After simple protein precipitation, the sample was directly injected into the introduction valve of the 2D-LC system. The 1 D column, playing a role of primary separation and preconcentration for complex plasma matrices, transferred the targets to the intermediate column. Following capture of targets on the middle column online, the analytes were transferred to the 2 D separation column by a six-port valve. The 2 D column, avoiding interference from the plasma matrix, completed further separation and quantification. An assistant pump was optimized for primary enrichment as well as final elution in the heart-cutting mode. The analytical time of amisulpride was 7.401 min. The accuracy was between 0.48 and 8.49%, while the intra- and inter-day precisions ranged from 0.9 to 3.1% and from 1.7% to 3.3%, respectively. The linear range of amisulpride was 48.15-2,407.59 ng/ml, while the extraction recovery was 98.7-101.3%. The strategy established in the study, which was successfully applied to therapeutic drug monitoring of amisulpride for routine clinical detection, displays high sensitivity, good repeatability, convenience and low cost.

Association of ABCB1 polymorphisms with lipid homeostasis and liver injury response to atorvastatin in the Chinese population.

The present research was to assess the relationship between ABCB1 (G2677T/A, C3435T) polymorphisms and lipid homeostasis as well as risk of liver injury induced by atorvastatin in in-patients from China. The lipid levels (total cholesterol, high-density lipoprotein, triglycerides) as well as metabolic enzymes of hepar (glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase, alkaline phosphatase, γ-glutamyl transpeptidase) in plasma for 162 patients were measured at baseline and after approximately 6 months of atorvastatin treatment. Polymorphisms of the ABCB1 gene were determined using the Snapshot technique. The associations between genetic polymorphisms and lipid levels as well as hepar indexes were evaluated at the end of medical treatment. Based on one-way ANOVA analysis, patients with the 2677GG or 3435TT genotypes showed a remarkable decrease in percentage when the level of TC was above 4.00 mmol·L-1, separately (P < 0.05). There was a significant decrease in percentage in the frequency of patients with the 2677GG genotype (low-density lipoprotein > 2.00 mmol·L-1) (P < 0.05). The level of glutamic-pyruvic transaminase in patients with the 2677GG or 3435CC genotype displayed a significantly increase in percentage, respectively (P < 0.05). The ABCB1 G-C haplotype carriers were associated with an increased risk of AILI. The results provide evidence for clinically individualised utilisation of atorvastatin for lipid homeostasis as well as risk of induced liver injury in the Chinese population.

Flexible construction of cellulose photonic crystal optical sensing nano-materials detecting organic solvents.

We developed a simple and efficient method to construct 3D and 2D opal and inverse opal cellulose photonic crystal films (CPCF) by embedding 3D or 2D polymethyl methacrylate (PMMA) colloidal arrays into carboxymethyl cellulose (CMC), respectively. The morphology and optical performance of CPCFs were characterized by SEM, diffraction spectra, Debye rings, and structural color. The brilliant structural colors of CPCFs are visible to the eye in the entire visible spectrum, and can be tuned by changing the particle diameters or the pore sizes. Attributed to decreased particle spacing and lower average refractive index caused by air spheres instead of polymer spheres, the stopbands of the inverse opal CPCFs blue-shifted. To the contrary, the particle spacing of 2D inverse opal CPCFs increased due to the losing of the connection force of 2D arrays, along with decreasing of Debye ring diameters. By alternately being exposed to organic solvents of methanol, acetonitrile, butanol, dioxane, and carbon tetrachloride, the 3D inverse opal CPCFs displayed an excellent sensing performance with instantaneously reversible color changes from violet to red. Their high stability and flexibility, efficient visual detection, and wide range of analytes promises a new opportunity for optical switching and sensing applications.

Perfluorocarbon Nanoparticles Mediated Platelet Blocking Disrupt Vascular Barriers to Improve the Efficacy of Oxygen-Sensitive Antitumor Drugs.

Currently, limited tumor drug permeation and poor oxygen perfusion are two major bottlenecks that significantly impair the efficacy of existing antitumor drugs, especially oxygen-sensitive antitumor drugs. One vital cause of these major bottlenecks is the abnormal tumor vessel barrier. To the best knowledge of the authors, platelets play a vital role in the maintenance of an abnormal tumor blood barrier through platelet-tumor interaction. Thus, platelet inhibition may present a new way to enhance drug delivery. In this study, it is originally discovered that perfluorotributylamine-based albumin nanoparticles (PFTBA@HSA) possess excellent platelet inhibiting abilities, which then selectively disrupt the tumor vessel barrier, resulting in a remarkably enhanced intratumoral drug accumulation. Interestingly enough, the tumor hypoxia is also obviously relieved by enhanced oxygen carrier red blood cell distribution and PFTBA@HSA infiltration in the tumors. Finally, the efficacy of oxygen-sensitive antitumor drugs is significantly amplified by PFTBA@HSA owing to enhanced drug permeation and relieved tumor hypoxia. Therefore, for the first time, it is demonstrated that PFTBA@HSA could be used as an effective way to improve the efficacy of existing tumor therapies by disrupting tumor vessel barriers through targeted platelet inhibition.

Purification and characterization of antibacterial compounds of Pseudoalteromonas flavipulchra JG1.

Pseudoalteromonas flavipulchra JG1 produces a protein PfaP and a range of small-molecule compounds with inhibitory activities against Vibrio anguillarum. The PfaP protein was purified from the extracellular products of JG1 by electroelution, and antibacterial activity was observed by an in-gel antibacterial assay. The complete amino acid sequence (694 aa) of PfaP was determined by de novo peptide sequencing and subsequent alignment with the proteome sequence of strain JG1. The calculated molecular mass of PfaP was 77.0 kDa. PfaP was 58 % identical to l-lysine oxidase AlpP of Pseudoalteromonas tunicata D2, and 54 % identical to the marinocine antimicrobial protein of Marinomonas mediterranea MMB-1. Five small molecules (compounds 1-5) with antibacterial activity, which were identified as p-hydroxybenzoic acid (1), trans-cinnamic acid (2), 6-bromoindolyl-3-acetic acid (3), N-hydroxybenzoisoxazolone (4) and 2'-deoxyadenosine (5), were purified by sequential column chromatography over silica gel, Sephadex LH-20 and RP-18 from ethyl acetate extract of strain JG1, and their structures were determined by NMR and MS. Brown compound 3, the only brominated compound, showed antibacterial activity against both Gram-positive and Gram-negative bacteria.

A single residue change in Vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (Scophthalmus maximus).

Vibrio harveyi hemolysin, an important virulence determinant in fish pathogenesis, was further characterized, and the enzyme was identified as a phospholipase B by gas chromatography. Site-directed mutagenesis revealed that a specific residue, Ser153, was critical for its enzymatic activity and for its virulence in fish.