RESUMEN
OBJECTIVE: To design DNA microarray and investigate the molecular anti-tumor mechanism of herbs of traditional Chinese medicine. METHOD: cDNA microarrays consisting of 56 probes representing 24 human cell cycle genes were constructed, Four anti-hepatocarcinoma herbs including Radix Linderae, Hebra Artemisiae Annuae, Radix Amebiae, Radix Astragli, were chosen. Effects of herbs on SMMC-7721 cell cycle were observed by flow cytometry assay. Effects of herbs on cell cycle gene expression in SMMC-7721 cells were analyzed by comparing hybridization of Dig-Labeled cDNAs from herb-treated cells and cDNAs from untreated cells. RESULT: Expressions of cell cycle geneswere changed in different degrees after herbs treated. Some genes were down-regulated and some genes were up-regulated. The changes in gene expression agreed with the results of flow cytometry assay. CONCLUSION: The results suggest that these herbs may have effects on cell cycle and DNA damage checkpoint genes which may be the mechanism of the herbs, and DNA microarray can be used to investigate the biological function of extracts of traditional Chinese medicine.
Asunto(s)
Antineoplásicos Fitogénicos/farmacología , Carcinoma Hepatocelular/patología , Medicamentos Herbarios Chinos/farmacología , Neoplasias Hepáticas/patología , Plantas Medicinales , Antineoplásicos Fitogénicos/aislamiento & purificación , Artemisia/química , Astragalus propinquus/química , Carcinoma Hepatocelular/metabolismo , Línea Celular Tumoral , Quinasa 4 Dependiente de la Ciclina , Inhibidor p16 de la Quinasa Dependiente de Ciclina/genética , Inhibidor p16 de la Quinasa Dependiente de Ciclina/metabolismo , Quinasas Ciclina-Dependientes/genética , Quinasas Ciclina-Dependientes/metabolismo , Medicamentos Herbarios Chinos/aislamiento & purificación , Amplificación de Genes , Perfilación de la Expresión Génica , Genes cdc/efectos de los fármacos , Humanos , Lindera/química , Lithospermum/química , Neoplasias Hepáticas/metabolismo , Análisis de Secuencia por Matrices de Oligonucleótidos/métodos , Plantas Medicinales/química , Antígeno Nuclear de Célula en Proliferación/genética , Antígeno Nuclear de Célula en Proliferación/metabolismo , Proteínas Proto-Oncogénicas/genética , Proteínas Proto-Oncogénicas/metabolismo , Fosfatasas cdc25/genética , Fosfatasas cdc25/metabolismoRESUMEN
OBJECTIVE: To screen and identify the differentially expressed genes in hepatocellular carcinoma cells SMMC-7721 responsing to the aqueous extract from dried powdered rhizomes of Typhonium giganteum (AEoTGE). METHOD: The response of hepatocellular carcinoma cells SMMC-7721 to AEoTGE was explored with the technique of mRNA differential display. RESULT: After hepatocarcinoma cells SMMC-7721 were treated by AEoTGE for 36 hours, 1 gene expression was upgrade and 1 gene expression was downgrade induced by AEoTGE. CONCLUSION: The research has provided important clues for the molecular mechanism of how hepatocarcinoma cells responseing to T. giganteum.