RESUMEN
The local gill production of corticotropin releasing hormone (crh) and crh-receptor (crhr) is hypothesized to play important roles during seawater (SW) and freshwater (FW) acclimation in euryhaline black porgy (Acanthopagrus schlegelii). The mRNA expression of crh, crhr, and Na +/K + -ATPase (a-nka) was examined in SW and FW diencephalon (Dien) and in the gills at different exposure time by Q-PCR analysis. The in situ hybridization results indicate that crh mRNA hybridization signals were more abundant in FW fish in the gigantocellular (PMgc) and parvocellular (PMpc) part of the magnocellular preoptic nucleus versus SW fish. The crh and crhr-expressing cells were located in basal cells of gill filament. Furthermore, in vitro dexamethasone (DEX) treatment could increase the crh-system in the gill. Increased transcripts of the crh-system in the gill via in vitro and in vivo CRH treatments suggest that CRH may regulate the system in a local manner. The a-Nka cells were localized in the filament and secondary lamellae mitochondria rich cells (MRCs) of FW fish at 8 h and 1 day. a-Nka cells were seen in both filament and lamellae in the FW but much less in SW fish indicating that gills play key roles in black porgy osmoregulation. Gill crh and crhr play important roles in the response to salinity stress.
RESUMEN
Black porgy is a marine euryhaline species with a capacity to cope with demands in a wide range of salinities and thus is a perfect model-fish to study osmoregulatory responses to salinity-acclimated processes and their hormonal control. The present study was performed to understand the regulatory changes in hormone, hormone receptors and important osmoregulatory genes in pituitary, gill, intestine and kidney in response to acute salinity stress. Transcript levels were analyzed by quantitative real-time PCR following acute salinity challenge by direct transfer of seawater (SW) acclimatized fish to fresh water (FWBP) and vice versa (SWBP). SW acclimation significantly increased plasma osmolality and intestine Na+/K+-ATPase (NKA) activity while FW acclimation increased plasma cortisol and branchial NKA activity. Plasma osmolality and chloride concentration decreased in FWBP whereas GH levels remained unchanged in both FWBP and SWBP. Comparative analysis of gene profiles between FWBP and SWBP showed that pituitary prolactin transcript increased significantly in FWBP. Prolactin receptor (PRLR) transcripts increased in gill of FWBP while it decreased in gill and kidney of SWBP. NKA transcripts increased in gill of both FWBP and SWBP, while it decreased in intestine of FWBP and increased in intestine and kidney of SWBP. Glucocorticoid receptor (GR) transcripts decreased in intestine and kidney of FWBP while it increased in gill and intestine of SWBP. No significant changes were observed in growth hormone receptor (GHR) transcripts of both FWBP and SWBP in pituitary, gill, intestine and kidney. Our current data demonstrated the correlation between PRLR gene expression in relation to FW adaptation, and GR gene expression in relation to SW adaptation in euryhaline black porgy. The results indicate that black porgy has an excellent osmoregulatory capacity and is capable of withstanding large variations in salinity.