RESUMEN
The mechanism of action of isoniazid (INH), a first-line antituberculosis drug, is complex, as mutations in at least five different genes (katG, inhA, ahpC, kasA, and ndh) have been found to correlate with isoniazid resistance. Despite this complexity, a preponderance of evidence implicates inhA, which codes for an enoyl-acyl carrier protein reductase of the fatty acid synthase II (FASII), as the primary target of INH. However, INH treatment of Mycobacterium tuberculosis causes the accumulation of hexacosanoic acid (C(26:0)), a result unexpected for the blocking of an enoyl-reductase. To test whether inactivation of InhA is identical to INH treatment of mycobacteria, we isolated a temperature-sensitive mutation in the inhA gene of Mycobacterium smegmatis that rendered InhA inactive at 42 degrees C. Thermal inactivation of InhA in M. smegmatis resulted in the inhibition of mycolic acid biosynthesis, a decrease in hexadecanoic acid (C(16:0)) and a concomitant increase of tetracosanoic acid (C(24:0)) in a manner equivalent to that seen in INH-treated cells. Similarly, INH treatment of Mycobacterium bovis BCG caused an inhibition of mycolic acid biosynthesis, a decrease in C(16:0), and a concomitant accumulation of C(26:0). Moreover, the InhA-inactivated cells, like INH-treated cells, underwent a drastic morphological change, leading to cell lysis. These data show that InhA inactivation, alone, is sufficient to induce the accumulation of saturated fatty acids, cell wall alterations, and cell lysis and are consistent with InhA being a primary target of INH.
Asunto(s)
Antituberculosos/farmacología , Bacteriólisis , Ácido Graso Sintasas/metabolismo , Isoniazida/farmacología , Mycobacterium smegmatis/metabolismo , Oxidorreductasas/metabolismo , Alelos , Proteínas Bacterianas , Farmacorresistencia Microbiana , Ácido Graso Sintasas/efectos de los fármacos , Ácido Graso Sintasas/genética , Ácidos Grasos/metabolismo , Calor , Mycobacterium smegmatis/efectos de los fármacos , Mycobacterium smegmatis/ultraestructura , Ácidos Micólicos/metabolismo , Oxidorreductasas/efectos de los fármacos , Oxidorreductasas/genética , Ácido Palmítico/metabolismoRESUMEN
Understanding the effects of the external environment on bacterial gene expression can provide valuable insights into an array of cellular mechanisms including pathogenesis, drug resistance, and, in the case of Mycobacterium tuberculosis, latency. Because of the absence of poly(A)+ mRNA in prokaryotic organisms, studies of differential gene expression currently must be performed either with large amounts of total RNA or rely on amplification techniques that can alter the proportional representation of individual mRNA sequences. We have developed an approach to study differences in bacterial mRNA expression that enables amplification by the PCR of a complex mixture of cDNA sequences in a reproducible manner that obviates the confounding effects of selected highly expressed sequences, e.g., ribosomal RNA. Differential expression using customized amplification libraries (DECAL) uses a library of amplifiable genomic sequences to convert total cellular RNA into an amplified probe for gene expression screens. DECAL can detect 4-fold differences in the mRNA levels of rare sequences and can be performed on as little as 10 ng of total RNA. DECAL was used to investigate the in vitro effect of the antibiotic isoniazid on M. tuberculosis, and three previously uncharacterized isoniazid-induced genes, iniA, iniB, and iniC, were identified. The iniB gene has homology to cell wall proteins, and iniA contains a phosphopantetheine attachment site motif suggestive of an acyl carrier protein. The iniA gene is also induced by the antibiotic ethambutol, an agent that inhibits cell wall biosynthesis by a mechanism that is distinct from isoniazid. The DECAL method offers a powerful new tool for the study of differential gene expression.
Asunto(s)
Amplificación de Genes , Regulación Bacteriana de la Expresión Génica/efectos de los fármacos , Biblioteca Genómica , Isoniazida/farmacología , Mycobacterium tuberculosis/genética , ARN Mensajero/genética , Secuencia de Bases , Cósmidos , Cartilla de ADN , Mycobacterium tuberculosis/efectos de los fármacos , Plásmidos , ARN Mensajero/análisis , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Ribosomas/metabolismoRESUMEN
Isoniazid (INH) is a highly effective drug used in the treatment and prophylaxis of Mycobacterium tuberculosis infections. Resistance to INH in clinical isolates has been correlated with mutations in the inhA, katG, and ahpC genes. In this report, we describe a new mechanism for INH resistance in Mycobacterium smegmatis. Mutations that reduce NADH dehydrogenase activity (Ndh; type II) cause multiple phenotypes, including (i) coresistance to INH and a related drug, ethionamide; (ii) thermosensitive lethality; and (iii) auxotrophy. These phenotypes are corrected by expression of one of two enzymes: NADH dehydrogenase and the NADH-dependent malate dehydrogenase of the M. tuberculosis complex. The genetic data presented here indicate that defects in NADH oxidation cause all of the mutant traits and that an increase in the NADH/NAD+ ratio confers INH resistance.
Asunto(s)
Antituberculosos/farmacología , Proteínas Bacterianas , Isoniazida/farmacología , Mycobacterium/genética , NADH Deshidrogenasa/genética , Secuencia de Aminoácidos , Farmacorresistencia Microbiana , Prueba de Complementación Genética , Malato Deshidrogenasa/genética , Pruebas de Sensibilidad Microbiana , Modelos Biológicos , Datos de Secuencia Molecular , Mutación , NAD/metabolismo , Oxidación-Reducción , Oxidorreductasas/metabolismo , Peroxidasas/metabolismo , Fenotipo , Quinonas/metabolismo , Homología de Secuencia de AminoácidoRESUMEN
Diaminopimelate (DAP) is used by bacteria for the synthesis of lysine. In many species of bacteria, including mycobacteria, DAP is also used for peptidoglycan biosynthesis. In this report we describe the cloning of the dapB gene encoding dihydrodipicolinate reductase (DHPR), which catalyzes a key branch point reaction in the bacterial DAP biosynthetic pathway, from Mycobacterium tuberculosis. Analyses of the DapB proteins from different bacterial species suggest that two different classes of DHPR enzymes may exist in bacteria.
Asunto(s)
Genes Bacterianos/genética , Mycobacterium tuberculosis/genética , Oxidorreductasas actuantes sobre Donantes de Grupo CH-CH , Oxidorreductasas/genética , Secuencia de Aminoácidos , Secuencia de Bases , Clonación Molecular , Dihidrodipicolinato-Reductasa , Modelos Químicos , Datos de Secuencia Molecular , Mycobacterium tuberculosis/enzimología , Filogenia , Mapeo Restrictivo , Análisis de Secuencia de ADN , Homología de Secuencia de AminoácidoRESUMEN
Genetic studies of Mycobacterium tuberculosis have been greatly hampered by the inability to introduce specific chromosomal mutations. Whereas the ability to perform allelic exchanges has provided a useful method of gene disruption in other organisms, in the clinically important species of mycobacteria, such as M. tuberculosis and Mycobacterium bovis, similar approaches have thus far been unsuccessful. In this communication, we report the development of a shuttle mutagenesis strategy that involves the use of long linear recombination substrates to reproducibly obtain recombinants by allelic exchange in M. tuberculosis. Long linear recombination substrates, approximately 40 to 50 kb in length, were generated by constructing libraries in the excisable cosmid vector pYUB328. The cosmid vector could be readily excised from the recombinant cosmids by digestion with PacI, a restriction endonuclease for which there exist few, if any, sites in mycobacterial genomes. A cosmid containing the mycobacterial leuD gene was isolated, and a selectable marker conferring resistance to kanamycin was inserted into the leuD gene in the recombinant cosmid by interplasmid recombination in Escherichia coli. A long linear recombination substrate containing the insertionally mutated leuD gene was generated by PacI digestion. Electroporation of this recombination substrate containing the insertionally mutated leuD allele resulted in the generation of leucine auxotrophic mutants by homologous recombination in 6% of the kanamycin-resistant transformants for both the Erdman and H37Rv strains of M. tuberculosis. The ability to perform allelic exchanges provides an important approach for investigating the biology of this pathogen as well as developing new live-cell M. tuberculosis-based vaccines.
Asunto(s)
Alelos , Mutagénesis Insercional/métodos , Mycobacterium tuberculosis/genética , Recombinación Genética/genética , Secuencia de Bases , Conjugación Genética , Cósmidos/genética , ADN Bacteriano/análisis , Escherichia coli/genética , Genes Bacterianos/genética , Marcadores Genéticos , Biblioteca Genómica , Leucina/biosíntesis , Datos de Secuencia MolecularRESUMEN
Insertional mutagenesis in Mycobacterium bovis BCG, a member of the slow-growing M. tuberculosis complex, was accomplished with transposons engineered from the Mycobacterium smegmatis insertion element IS1096. Transposons were created by placing a kanamycin resistance gene in several different positions in IS1096, and the resulting transposons were electroporated into BCG on nonreplicating plasmids. These analyses demonstrated that only one of the two open reading frames was necessary for transposition. A library of insertions was generated. Southern analysis of 23 kanamycin-resistant clones revealed that the transposons had inserted directly, with no evidence of cointegrate formation, into different restriction fragments in each clone. Sequence analysis of nine of the clones revealed junctional direct 8-bp repeats with only a slight similarity in target sites. These results suggest that IS1096-derived transposons transposed into the BCG genome in a relatively random fashion. Three auxotrophs, two for leucine and one for methionine, were isolated from the library of transposon insertions in BCG. They were characterized by sequencing and found to be homologous to the leuD gene of Escherichia coli and a sulfate-binding protein of cyanobacteria, respectively. When inoculated intravenously into C57BL/6 mice, the leucine auxotrophs, in contrast to the parent BCG strain or the methionine auxotroph, showed an inability to grow in vivo and were cleared within 7 weeks from the lungs and spleen.
Asunto(s)
Mutagénesis Insercional/métodos , Mycobacterium bovis/crecimiento & desarrollo , Mycobacterium bovis/genética , Animales , Secuencia de Bases , Elementos Transponibles de ADN , Genes Bacterianos/genética , Prueba de Complementación Genética , Leucina/metabolismo , Pulmón/microbiología , Masculino , Metionina/metabolismo , Ratones , Ratones Endogámicos C57BL , Datos de Secuencia Molecular , Mutación , Análisis de Secuencia de ADN , Bazo/microbiología , Transformación Genética , TuberculosisRESUMEN
The increasing incidence of multiple-drug-resistant mycobacterial infections indicates that the development of new methods for treatment of mycobacterial diseases should be a high priority. meso-Diaminopimelic acid (DAP), a key component of a highly immunogenic subunit of the mycobacterial peptidoglycan layer, has been implicated as a potential virulence factor. The mycobacterial DAP biosynthetic pathway could serve as a target for design of new antimycobacterial agents as well as the construction of in vivo selection systems. We have isolated the asd, dapA, dapB, dapD, and dapE genes involved in the DAP biosynthetic pathway of Mycobacterium bovis BCG. These genes were isolated by complementation of Escherichia coli mutations with an expression library of BCG DNA. Our analysis of these genes suggests that BCG may use more than one pathway for biosynthesis of DAP. The nucleotide sequence of the BCG dapB gene was determined. The activity of the product of this gene in Escherichia coli provided evidence that the gene may encode a novel bifunctional dihydrodipicolinate reductase and DAP dehydrogenase.
Asunto(s)
Ácido Diaminopimélico/metabolismo , Genes Bacterianos , Mycobacterium bovis/genética , Secuencia de Aminoácidos , Secuencia de Bases , Secuencia Conservada , Escherichia coli/genética , Prueba de Complementación Genética , Datos de Secuencia Molecular , Mutación , Mycobacterium bovis/metabolismo , Sistemas de Lectura AbiertaRESUMEN
Diaminopimelic acid (DAP) is a major component of the peptidoglycan layer of the mycobacterial cell wall. The mycobacterial cell wall has been implicated as a potential virulence factor and is highly immunogenic. The pathway for biosynthesis of DAP may serve as a target in the design of antimycobacterial agents and construction of in vivo selection systems. Despite its significance, this biosynthetic pathway is poorly understood in mycobacteria. In order to develop a better understanding of mycobacterial DAP biosynthesis, the aspartate semialdehyde dehydrogenase (asd) genes of Mycobacterium smegmatis, bacille Calmette-Guerin (BCG), Mycobacterium avium, Mycobacterium leprae, and Mycobacterium tuberculosis were isolated. The M. smegmatis asd gene was isolated by complementation in Escherichia coli. This gene was then used to isolate the asd genes from other mycobacteria. The asd-complementing fragments from BCG and M. smegmatis were sequenced. An open reading frame upstream of the mycobacterial asd gene was identified as the mycobacterial aspartokinase gene (ask). Primer extension analysis revealed that the only transcriptional start in this region is found 5' of the ask gene. This observation indicates that the mycobacterial ask and asd genes are in an operon.