Knockdown of circSlc8a1 inhibited the ferroptosis in the angiotensin II treated H9c2 cells via miR-673-5p/TFRC axis.

BACKGROUND: This study aimed to investigate the role of circSlc8a1 in cardiac hypertrophy (CH), a pathological change in various cardiovascular diseases. METHODS: An in vitro CH model was established using angiotensin II (AngII) treated H9c2 cells, followed by western blotting and RT-qPCR for detecting relative expressions. Cell viability and proliferation were analyzed using CCK-8 and EdU assays, while lactate dehydrogenase (LDH), reactive oxygen species (ROS), glutathione (GSH), and iron levels were determined using corresponding kits. Moreover, dual-luciferase reporter and RNA pull-down assays were performed to demonstrate whether miR-673-5p is bound to circSlc8a1 or transferrin receptor (TFRC). RESULTS: The results indicated that the expressions of circSlc8a1 and TFRC were increased, while miR-673-5p was decreased in the AngII treated H9c2 cells. The ferroptosis inhibitor treatment decreased the atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and ß-major histocompatibility complex (ß-MHC) protein expressions, and circSlc8a1 expressions. Knocking down of circSlc8a1 inhibited promoted the cell viability and proliferation, increased the GSH content, glutathione peroxidase 4, and solute carrier family 7 member 11 protein expressions, and decreased the LDH, ROS, iron levels, and RAS protein expressions. The MiR-673-5p inhibitor antagonized the role of si-circSlc8a1, and the over-expressed TFRC reversed the miR-673-5p mimicking effects in AngII treated H9c2 cells. CONCLUSION: CircSlc8a1 promoted the ferroptosis in CH via regulating the miR-673-5p/TFRC axis.

PCDHA1 High Expression is Associated With Poor Prognosis and Correlated With Immune Cell Infiltration in Breast Cancer.

INTRODUCTION: Breast cancer (BC) remains one of the biggest threats to women's health. Protocadherin gene Protocadherin Alpha 1 (PCDHA1) is abnormally highly expressed in breast cancer tissues. However, the biological role of PCDHA1 in breast cancer has not been fully elucidated and the relationship with the immune microenvironment needs to be further studied. MATERIALS AND METHODS: TCGA-BRCA gene expression profiles were used to characterize PCDHA1. Kaplan-Meier method was used to estimate PCDHA1 prognosis potential. Gene set enrichment analysis (GSEA) analysis was performed to determine the signaling pathways altered by PCDHA1 aberrant expression. The correlations between PCDHA1 and immune cell infiltration levels were analyzed by CIBERSORT. Wilcoxon's rank-sum test was used to identify chemokine and chemokine receptors significantly associated with PCDHA1. The CCK8 assay and the transwell invasion assay were occupied to evaluate the effect of PCDHA1 overexpression on BC cells. RESULTS: Survival analysis revealed PCDHA1 overexpression was associated with poor prognosis in BC. Enrichment analysis uncovered several metabolism pathways were activated by PCDHA1 overexpression. Moreover, PCDHA1 was positively correlated with activated NK cells but negatively correlated with resting NK cells infiltration. In addition, chemokines CCL28, CXCL17, and receptor CCR9 expression were associated with PCDHA1 overexpression. The CCK8 assay and the transwell invasion assay proved that PCDHA1 overexpression enhanced MCF-7 and MDA-MB-231 cell proliferation and invasion. CONCLUSION: This study demonstrated that PCDHA1 effectively predicted BC prognosis. Upregulated PCDHA1 activated metabolism pathways, and promoted NK cells and chemokines. PCDHA1 overexpression enhanced BC cell proliferation and invasion. Therefore, an understanding of PCDHA1's function in BC may yield insights into the mechanisms of BC development.

Lactate and Lactylation in the Brain: Current Progress and Perspectives.

As the final product of glycolysis, lactate features not only as an energy substrate, a metabolite, and a signaling molecule in a variety of diseases-such as cancer, inflammation, and sepsis-but also as a regulator of protein lactylation; this is a newly proposed epigenetic modification that is considered to be crucial for energy metabolism and signaling in brain tissues under both physiological and pathological conditions. In this review, evidence on lactylation from studies on lactate metabolism and disease has been summarized, revealing the function of lactate and its receptors in the regulation of brain function and summarizing the levels of lactylation expression in various brain diseases. Finally, the function of lactate and lactylation in the brain and the potential mechanisms of intervention in brain diseases are presented and discussed, providing optimal perspectives for future research on the role of lactylation in the brain.

Metal-oxide-semiconductor resistive gas sensors for fish freshness detection.

Fish are prone to spoilage and deterioration during processing, storage, or transportation. Therefore, there is a need for rapid and efficient techniques to detect and evaluate fish freshness during different periods or conditions. Gas sensors are increasingly important in the qualitative and quantitative evaluation of high-protein foods, including fish. Among them, metal-oxide-semiconductor resistive (MOSR) sensors with advantages such as low cost, small size, easy integration, and high sensitivity have been extensively studied in the past few years, which gradually show promising practical application prospects. Herein, we take the detection, classification, and assessment of fish freshness as the actual demand, and summarize the physical and chemical changes of fish during the spoilage process, the volatile marker gases released, and their production mechanisms. Then, we introduce the advantages, performance parameters, and working principles of gas sensors, and summarize the MOSR gas sensors aimed at detecting different kinds of volatile marker gases of fish spoiling in the last 5 years. After that, this paper reviews the research and application progress of MOSR gas sensor arrays and electronic nose technology for various odor indicators and fish freshness detection. Finally, this review points out the multifaceted challenges (sampling system, sensing module, and pattern recognition technology) faced by the rapid detection technology of fish freshness based on metal oxide gas sensors, and the potential solutions and development directions are proposed from the view of multidisciplinary intersection.

Relationship between religion and redress relief among Japanese American World War II incarceration survivors.

Soon after Japan attacked Pearl Harbor and the United States entered World War II, more than 110,000 Japanese Americans (two-thirds U.S. citizens) were rounded up and ordered into desolate incarceration (internment) camps based only on their ethnic heritage. More than 40 years later, the U.S. government acknowledged that the Japanese American incarceration was unjustified and provided a formal apology and monetary award to surviving incarcerees. The present study investigates the relationship between religious affiliation (Buddhist and Christian) and subsequent perceptions of relief associated with the government's belated redress. Based on a national sample of U.S.-born Japanese American former incarcerees (N = 454), Buddhist incarcerees reported greater relief from receiving redress than Christians. Across religious affiliations, older incarcerees and those with lower income reported more relief. Both Buddhist and Christian respondents who perceived more Japanese American incarceration-related physical suffering, and those who believed in a just world, experienced greater relief. In addition, Buddhists who more strongly believed their lives are controlled by unpredictable fate/fortune, and Christians who more strongly believed their lives were controlled by powerful others experienced greater redress relief. Findings suggest the role of religious frameworks in shaping the restorative capacity of belated reparative acts following historical racial trauma. (PsycInfo Database Record (c) 2022 APA, all rights reserved).

Gas sensing materials roadmap.

Gas sensor technology is widely utilized in various areas ranging from home security, environment and air pollution, to industrial production. It also hold great promise in non-invasive exhaled breath detection and an essential device in future internet of things. The past decade has witnessed giant advance in both fundamental research and industrial development of gas sensors, yet current efforts are being explored to achieve better selectivity, higher sensitivity and lower power consumption. The sensing layer in gas sensors have attracted dominant attention in the past research. In addition to the conventional metal oxide semiconductors, emerging nanocomposites and graphene-like two-dimensional materials also have drawn considerable research interest. This inspires us to organize this comprehensive 2020 gas sensing materials roadmap to discuss the current status, state-of-the-art progress, and present and future challenges in various materials that is potentially useful for gas sensors.

Catalpol Protects Against Spinal Cord Injury in Mice Through Regulating MicroRNA-142-Mediated HMGB1/TLR4/NF-κB Signaling Pathway.

Background: Spinal cord injury (SCI) is a devastating condition that leads to paralysis, disability and even death in severe cases. Inflammation, apoptosis and oxidative stress in neurons are key pathogenic processes in SCI. Catalpol (CTP), an iridoid glycoside extracted from Rehmannia glutinosa, has many pharmacological activities, such as anti-inflammatory, anti-oxidative and anti-apoptotic properties. Purpose: Here, we investigated whether CTP could exert neuroprotective effects against SCI, and explored the underlying mechanism involved. Methods: SCI was induced by a weight-drop device and treated with CTP (10 mg and 60 mg/kg). Then the locomotor function of SCI mice was evaluated by the BBB scores, spinal cord edema was measured by the wet/dry weight method, oxidative stress markers and inflammatory factors were detected by commercial kits and neuronal death was measured by TUNEL staining. Moreover, the microRNA expression profile in spinal cords from mice following SCI was analyzed using miRNA microarray. In addition, reactive oxygen species (ROS) generation, inflammatory response and cell apoptosis were detected in murine microglia BV2 cells under oxygen-glucose deprivation (OGD) and CTPtreatment. Results: Our data showed that CTP treatment could improve the functional recovery, as well as suppress the apoptosis, alleviate inflammatory and oxidative response in SCI mice. In addition, CTP was found to be up-regulated miR-142 and the protective effects of CTP on apoptosis, inflammatory and oxidative response may relate to its regulation of HMGB1/TLR4/NF-κB pathway through miR-142. Conclusion: Our findings suggest that CTP may protect the spinal cord from SCI by suppression of apoptosis, oxidative stress and inflammatory response via miR-142/HMGB1/TLR4/NF-κB pathway.

The Japanese American wartime incarceration: Examining the scope of racial trauma.

Ten weeks after the 1941 Japanese military attack on Pearl Harbor, Hawaii, the U.S. government authorized the removal of more than 110,000 Japanese American men, women, and children from their homes in Western portions of the country to incarceration camps in desolate areas of the United States. The mass incarceration was portrayed as necessary to protect the country from potential acts of espionage or sabotage that might be committed by someone of Japanese ancestry. However, an extensive government review initiated in 1980 found no evidence of military necessity to support the removal decision and concluded that the incarceration was a grave injustice fueled by racism and war hysteria. The Japanese American wartime experience represents a powerful case example of race-based historical trauma. This article describes the consequences of the incarceration for Japanese Americans during and after their unjust imprisonment, their coping responses and healing strategies, as well as the impacts of receiving governmental redress more than four decades after the war's end. Examination of this specific event provides a perspective for understanding the long-term, radiating effects of racial trauma and the process of healing, over a broad arc of time and across social contexts. Current relevance of the Japanese American incarceration and implications for the field of psychology are discussed. (PsycINFO Database Record (c) 2019 APA, all rights reserved).

Depression, Loneliness, and Suicide Risk among Latino College Students: A Test of a Psychosocial Interaction Model.

This study tested a psychosocial model of suicide risk in a sample of 156 Latino college students. Specifically, depression and loneliness were hypothesized to be important predictors of suicide risk (namely, hopelessness and suicidal behaviors) in Latino students. Results of conducting regression analyses indicated that, independent of age and gender, depression and loneliness were significant predictors of both indices of suicide risk examined in the present study. It is noteworthy that within the psychosocial predictor set of depression and loneliness, depression was consistently found to be nearly twice as strong a predictor than was loneliness. Moreover, we found evidence for a significant depression-loneliness interaction effect in predicting suicide risk. That is, the highest level of suicide risk was found among dysphoric Latino students who were also socially isolated. Our findings indicate that depression and loneliness are important factors to consider in understanding suicide risk among Latino college students.

Synthesis and acetone sensing properties of ZnFe2O4/rGO gas sensors.

Hollow spheres of pure ZnFe2O4 and of composites of ZnFe2O4 and reduced graphene oxide (rGO) with different rGO content were prepared via a simple solvothermal method followed by a high-temperature annealing process in an inert atmosphere. The X-ray diffraction analysis confirmed that the introduction of rGO had no effect on the spinel structure of ZnFe2O4. In addition, the results of field-emission scanning electron microscopy and (high-resolution) transmission electron microscopy indicated that the synthesized samples had the structure of hollow spheres distributed uniformly onto rGO nanosheets. The diameters of the spheres were determined as about 600-1000 nm. The gas sensing test revealed that the introduction of rGO improved the performance of the sensing of acetone to low concentration, and the ZnFe2O4/rGO composite gas sensor containing 0.5 wt % of rGO exhibited a high sensitivity in sensing test using 0.8-100 ppm acetone at 200 °C. The response of the 0.5 wt % ZnFe2O4/rGO sensor to 0.8 ppm acetone was 1.50, and its response to 10 ppm acetone was 8.18, which is around 2.6 times more pronounced than the response of pure ZnFe2O4 (10 ppm, 3.20). Moreover, the sensor showed a wide linear range and good selectivity.

How Future Orientation is Associated with Depressive Symptoms in Multiethnoracial Adults: Basic Psychological Needs as a Mediator.

We examined the role of basic psychological needs as a mediator of the association between future orientation and depressive symptoms in a sample of 202 (159 female and 43 male) multiethnoracial adults. Multiple mediation analysis with 10,000 bootstraps was conducted to test for mediation. The association between future orientation and depressive symptoms was found to be accounted for by dimensions of basic psychological needs. Specifically, future orientation was negatively related to depressive symptoms through positive associations involving autonomy and competence, but not relatedness. The present findings are the first to not only point to the importance of examining future orientation in understanding depressive symptoms in multiethnoracial adults, but they are also the first to suggest possible mechanisms by which believing in a changeable future might foster stronger satisfaction of basic psychological needs, especially autonomy and competence, that might help multiethnoracials garner greater protection when encountering stressful situations in their lives.

Perceptions of Sibling Relationships and Birth Order among Asian American and European American Emerging Adults.

Drawing from an ecological systems framework, we qualitatively explored how Confucian-heritage Asian American emerging adults compared with non-Hispanic European American emerging adults on views of sibling relationships and birth order. Thematic analysis of 48 semi-structured interviews revealed positive sibling relationship themes for both ethnocultural groups: mutual support, companionship, and appreciation; comfort from shared burden of negative parental interactions; and pride in one another. Birth-order themes were also similar across the groups. First-borns overall reported a strong pressure to be a role model to later-borns, provide sibling care, assume family responsibilities, and not expect to rely on younger siblings. Despite these similarities, Asian American first-borns were unique in taking comfort in having siblings who shared a less traditional Asian cultural perspective than their parents. They also described additional pressure from being the oldest within an immigrant family.

Alveolar soft-part sarcoma in the left forearm with cardiac metastasis: A case report and literature review.

Alveolar soft-part sarcoma (ASPS) is an uncommon soft-tissue neoplasm, which is commonly found in the deep soft tissues of the extremities, with a propensity for recurrence and metastasis. However, the metastasis of ASPS to the heart is exceedingly rare. The present study reports such a rare case with cardiac metastasis. Using computed tomography, a 37-year-old man was diagnosed with brain, lung and spleen metastases from a previously treated ASPS in the left forearm. Cardiac metastasis was then diagnosed 1 month later. Despite chemotherapy and palliative whole-brain radiotherapy, the patient succumbed to the disease shortly after. This case suggests that the widespread metastases and cardiac involvement of ASPS may result in a poor outcome.

Concentration of plasma neutrophil gelatinase-associated lipocalin in patients with chronic kidney disease.

BACKGROUND: The inclining incidence of chronic kidney disease which has led to high mortality and immense medical burden over the past decades has become a distressing concern in epidemiology. Unfortunately, the number of biomarkers that allow the monitoring of chronic kidney disease (CKD) is limited. Neutrophil gelatinase-associated lipocalin (NGAL) is an emerging biomarker which has been shown to be able to diagnose kidney injuries. METHODS: Eighty-one nondiabetic patients with chronic kidney disease, stage 2 to 5, were recruited for this study, and 17 healthy volunteers with eGFR greater than 90 mL/minute/1.73m(2) were selected as the control group. RESULTS: Our study demonstrated that the pNGAL level is elevated during CKD, and the pNGL level has a strong correlation with the concentration of sCr and eGFR. CONCLUSIONS: Plasma neutrophil gelatinase-associated lipocalin is a potent tool in the diagnosis of chronic kidney diseases and is shown to have high correlation with serum creatinine and estimated glomerular filtration rate.