RESUMEN
Allograft inflammatory factor-1 (AIF-1) is an inflammation-related protein mainly produced by immune cells, such as monocyte/macrophages and activated T lymphocytes. It is essential for the survival and proinflammatory activity of immune cells. However, the function of AIF-1 in chicken still has not been defined. In the present study, AIF-1-like (AIF1L) gene was identified in Luning chicken. Bioinformatics analysis revealed that the molecular weight of the chicken AIF-1 protein was 16290.8 Da. AIF1L contained a Ca2+ binding EF hand and could interact with actin filament. Its transcript was found in all tested tissues including spleen, brain, heart, kidney, liver, thymus, bursa of Fabricius, lung, and a relative low-level expression was detected in leg muscle. Furthermore, AIF1L expression in peripheral blood lymphocyte was depressed in a dose-dependent manner with cadmium exposure and peripheral blood lymphocyte viability decrease displayed a similar pattern with AIF1L expression. The results indicated that newly identified chicken AIF1L might be associated with lymphocyte viability.
Asunto(s)
Proteínas Aviares/genética , Proteínas de Unión al Calcio/genética , Pollos/genética , Proteínas de Microfilamentos/genética , Animales , Proteínas Aviares/química , Proteínas Aviares/metabolismo , Cadmio/toxicidad , Proteínas de Unión al Calcio/química , Proteínas de Unión al Calcio/metabolismo , Supervivencia Celular/efectos de los fármacos , Expresión Génica/efectos de los fármacos , Linfocitos/efectos de los fármacos , Proteínas de Microfilamentos/química , Proteínas de Microfilamentos/metabolismo , Reacción en Cadena en Tiempo Real de la Polimerasa , Pruebas de ToxicidadRESUMEN
This research communication describes the profile of gene expression related to the synthesis of yak milk as determined via quantitative reverse transcription polymerase chain reaction (RT-qPCR). Significant up-regulation during lactation were observed in genes related to fatty acid (FA) uptake from blood (LPL, CD36), intracellular FA transport (FABP3), intracellular FA activation of long- and short-chain FAs (ACSS1, ACSS2, ACSL1), de novo synthesis (ACACA), desaturation (SCD), triacyglycerol (TAG) synthesis (AGPAT6, GPAM, LPIN1), lipid droplet formation (PLIN2, BTN1A1, XDH), ketone body utilisation (BDH1, OXCT1), and transcription regulation (THRSP, PPARGC1A). In particular, intracellular de novo FA synthesis (ACSS2, ACACA, and FABP3) and TAG synthesis (GPAM, AGPAT6, and LPIN1), whose regulation might be orchestrated as part of the gene network under the control of SERBF1 in the milk fat synthesis process, were more activated compared to levels in dairy cows. However, the genes involved in lipid droplet formation (PLIN2, XDH, and BTN1A1) were expressed at lower levels compared to those in dairy cows, where these genes are mainly controlled by the PPARG regulator.
Asunto(s)
Bovinos/metabolismo , Expresión Génica , Lactancia/fisiología , Lípidos/biosíntesis , Glándulas Mamarias Animales/metabolismo , Leche/química , Adaptación Fisiológica , Altitud , Animales , Bovinos/genética , China , Ácidos Grasos/biosíntesis , Ácidos Grasos/metabolismo , Femenino , Regulación de la Expresión Génica , Lípidos/genética , Lipogénesis/genética , PPAR gamma/fisiología , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/veterinaria , Especificidad de la Especie , Proteína 1 de Unión a los Elementos Reguladores de Esteroles/fisiología , Transcriptoma/fisiología , TriglicéridosRESUMEN
Secreted frizzled related protein 5 (SFRP5), an anti-inflammatory adipokine, is relevant to the adipocyte differentiation. In order to clarify its role in regulating intramuscular fat (IMF) deposition in Tibetan chicken, the full-length sequence of the Tibetan chicken SFRP5 gene was cloned. The relative expression of SFRP5 gene was detected using quantitative RT-PCR in various tissues of 154 days old Tibetan chicken, as well as in breast muscle, thigh muscle, and adipose tissue at different growth stages. The results showed that SFRP5 gene was expressed in all examined tissues but highly enriched in adipose tissue. Temporal expression profile showed that the expression of SFRP5 was gradually decreased in breast muscle, but was fluctuated in thigh muscle and adipose tissue with the growth of Tibetan chicken. Furthermore, correlation analysis demonstrated that the expression of SFRP5 in breast muscle, thigh muscle and adipose tissue was correlated with IMF content at different levels. The results indicated that Tibetan chicken SFRP5 is involved in IMF deposition.