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In this study, we used multilayer graphene oxide (GO) obtained by anodic oxidation of graphite powder in 83% sulfuric acid. The modification of GO was carried out by its interaction with hexamethylenediamine (HMDA) according to the mechanism of nucleophilic substitution between the amino group of HMDA (HMDA) and the epoxy groups of GO, accompanied by partial reduction of multilayer GO and an increase in the deformation of the carbon layers. The structure and properties of modified HMDA-GO were characterized using research methods such as scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy and Raman spectroscopy. The conducted studies show the effectiveness of using HMDA-OG for modifying epoxy composites. Functionalizing treatment of GO particles helps reduce the free surface energy at the polymer-nanofiller interface and increase adhesion, which leads to the improvement in physical and mechanical characteristics of the composite material. The results demonstrate an increase in the strength and elastic modulus in bending by 48% and 102%, respectively, an increase in the impact strength by 122%, and an increase in the strength and elastic modulus in tension by 82% and 47%, respectively, as compared to the pristine epoxy composite which did not contain GO-HMDA. It has been found that the addition of GO-HMDA into the epoxy composition initiates the polymerization process due to the participation of reactive amino groups in the polymerization reaction, and also provides an increase in the thermal stability of epoxy nanocomposites.
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Within the framework of this study, Ni-based composite electrochemical coatings (CECs) modified with multilayer graphene oxide (GO) processed using microwave radiation have been deposited. The process of these coatings' electrodeposition in the potentiodynamic mode has been studied. The structure of Ni-GO and Ni-GO (MW) CECs has been studied using X-ray phase analysis (XPA) and scanning electron microscopy (SEM).It has been shown that the addition of GO into a nickel deposit contributes to the formation of uniform fine-grained coatings. As a result, the microhardness of the Ni-GO (MW) CECs increases by 1.40 times compared to Ni without GO. The corrosion-electrochemical behavior of nickel CECs in 0.5 M H2SO4 solution was researched. It was established that the corrosion rate of the nickel-GO (MW) CEC in 3.5% NaCl decreases by about 1.70 times in contrast to unmodified nickel coatings. This effect is due to the absence of agglomeration of the graphene oxide in the volume of the nickel matrix and the impermeability of GO particles to the corrosive environment.
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We present a collection of 295 gauge locations in mountainous Central Asia with norm discharge as well as time series of river discharge from 135 of these locations collected from hydrological yearbooks in Central Asia. Time series have monthly, 10-day and daily temporal resolution and are available for different duration. A collection of third-party data allows basin characterization for all gauges. The time series data is validated using standard quality checks. Norm discharge is validated against literature values and by using a water balance approach. The novelty of the data consists in the combination of discharge time series and gauge locations for mountainous rivers in Central Asia which is not available anywhere else. The geo-located discharge time series can be used for water balance modelling and training of forecast models for river runoff in mountainous Central Asia.
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The possibility of using graphene oxide as a modifying additive for polymer fiber-reinforced composites based on epoxy resin and basalt roving has been studied. The content of graphene oxide in the system has been experimentally selected, which has the best effect on the physico-mechanical properties of the obtained polymer composite material. The efficiency of the modification of the graphene oxide surface with APTES finishing additives and aminoacetic acid, which provides chemical interaction at the polymer matrix-filler interface, has been considered. The influence of graphene oxide and functionalizing additives on the polymer curing process was investigated using the thermometric method and differential scanning calorimetry.
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Metamorphosis in the insect larva is associated with disintegration, engulf and digestion of larval tissues. These processes are accompanied by a significant shift in physiological parameters like high activity of hydrolytic enzymes and decrease of pH. In the way, the metamorphosing larva resembles the processes occurring in the wound at the stage of inflammation. Based on this thesis, we put forward the idea of the possibility of using insect phagocytes in the wound treatment. The search for a suitable insect cell line and the study of its properties were the purpose of the work. The abilities of insect phagocytes to retain viability and functional activity under conditions physiological for humans were also investigated. We found that blue blowfly Calliphora vicina larvae had histolysocytes, a specialized population of professional phagocytes involved in the histolysis. In vitro, histolysocytes possess high phagocytic activity to fragments of vertebrate soft tissues and debris. These cells retain viability and functional activity for a long time under conditions that are physiological for vertebrate cells. Moreover histolysocytes can realize the humoral control over the bacteria through the synthesis of antimicrobial peptides. So histolysocytes have the potential to be used as xenogeneic phagocytes in the wound treatment. The data obtained allow proceeding to experiments on laboratory animals for studying the effect of such therapy on the wound healing process.
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Fagocitos/fisiología , Cicatrización de Heridas , Animales , Antiinfecciosos/farmacología , Diferenciación Celular/efectos de los fármacos , Pollos , Dípteros , Hemocitos/efectos de los fármacos , Hemocitos/fisiología , Cuerpos de Inclusión/efectos de los fármacos , Cuerpos de Inclusión/ultraestructura , Modelos Biológicos , Fagocitos/ultraestructura , Fagocitosis/efectos de los fármacos , Pupa/efectos de los fármacos , Pupa/fisiología , Porcinos , Cicatrización de Heridas/efectos de los fármacosRESUMEN
Nickel-based composite electrochemical coatings (CEC) modified with multilayer graphene oxide (GO) were obtained from a sulfate-chloride electrolyte in the reverse electrolysis mode. The microstructure of these CECs was investigated by X-ray phase analysis and scanning electron microscopy. The corrosion-electrochemical behavior of nickel-GO composite coatings in a 0.5 M solution of H2SO4was studied. Tests in a 3.5% NaCl solution showed that the inclusion of GO particles into the composition of electrolytic nickel deposits makes their corrosion rate 1.40-1.50 times less.
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In this article, amino functionalized multiwalled carbon nanotubes (MWCNTs) were prepared by chemical modification of the surface of a MWCNTs using γ-aminopropyltriethoxysilane (APTES) and dispersed into the epoxy composition. The modifying agent (APTES) was directly deposited on the MWCNTs surfaces. For the functionalization of MWCNTs, was used not the APTES concentrate, as it had been described in previous works, but its freshly prepared aqueous solution. Properties of APTES-treated MWCNTs were characterized by transmission electron microscope (TEM), Raman spectroscopy and FT-IR. The results showed that the functionalization and chemical compatibility of APTES-treated MWCNTs with epoxy composition provides their best dispersion in the epoxy composition, had important influence on curing behavior, structure and physicochemical properties of the epoxy composites plasticized with trichloroethyl phosphate. The results showed that the functionalization and chemical compatibility of APTES-treated MWCNTs with epoxy composition provides increased of physicomechanical properties of epoxy composites: bending stress increases by 194% and bending modulus increases by 137%, the tensile strength increases by 108% and the tensile elastic modulus increases by 52%, impact strength increases by 300%, in comparison with plasticized epoxy composite that does not contain MWCNTs.
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PURPOSE: The aim of this study is to improve the anti-biofilm activity of antibiotics. We hypothesized that the antimicrobial peptide (AMP) complex of the host's immune system can be used for this purpose and examined the assumption on model biofilms. METHODS: FLIP7, the AMP complex of the blowfly Calliphora vicina containing a combination of defensins, cecropins, diptericins and proline-rich peptides was isolated from the hemolymph of bacteria-challenged maggots. The complex interaction with antibiotics of various classes was studied in biofilm and planktonic cultures of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Acinetobacter baumannii by the checkerboard method using trimethyl tetrazolium chloride cell viability and crystal violet biofilm eradication assays supplemented with microscopic analysis. RESULTS: We found that FLIP7 demonstrated: high synergy (fractional inhibitory concentration index <0.25) with meropenem, amikacin, kanamycin, ampicillin, vancomycin and cefotaxime; synergy with clindamycin, erythromycin and chloramphenicol; additive interaction with oxacillin, tetracycline, ciprofloxacin and gentamicin; and no interaction with polymyxin B. The interaction in planktonic cell models was significantly weaker than in biofilms of the same strains. The analysis of the dose-effect curves pointed to persister cells as a likely target of FLIP7 synergistic effect. The biofilm eradication assay showed that the effect also caused total destruction of S. aureus and E. coli biofilm materials. The effect allowed reducing the effective anti-biofilm concentration of the antibiotic to a level well below the one clinically achievable (2-3 orders of magnitude in the case of meropenem, ampicillin, cefotaxime and oxacillin). CONCLUSION: FLIP7 is a highly efficient host antimicrobial system helping antibiotics to overcome biofilm barriers through persisters' sensitization and biofilm material destruction. It is promising for the treatment of biofilm infections as an adjuvant of various small-molecule antibiotics.
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Biofilms, sedimented microbial communities embedded in a biopolymer matrix cause vast majority of human bacterial infections and many severe complications such as chronic inflammatory diseases and cancer. Biofilms' resistance to the host immunity and antibiotics makes this kind of infection particularly intractable. Antimicrobial peptides (AMPs) are a ubiquitous facet of innate immunity in animals. However, AMPs activity was studied mainly on planktonic bacteria and little is known about their effects on biofilms. We studied structure and anti-biofilm activity of AMP complex produced by the maggots of blowfly Calliphora vicina living in environments extremely contaminated by biofilm-forming germs. The complex exhibits strong cell killing and matrix destroying activity against human pathogenic antibiotic resistant Escherichia coli, Staphylococcus aureus and Acinetobacter baumannii biofilms as well as non-toxicity to human immune cells. The complex was found to contain AMPs from defensin, cecropin, diptericin and proline-rich peptide families simultaneously expressed in response to bacterial infection and encoded by hundreds mRNA isoforms. All the families combine cell killing and matrix destruction mechanisms, but the ratio of these effects and antibacterial activity spectrum are specific to each family. These molecules dramatically extend the list of known anti-biofilm AMPs. However, pharmacological development of the complex as a whole can provide significant advantages compared with a conventional one-component approach. In particular, a similar level of activity against biofilm and planktonic bacteria (MBEC/MIC ratio) provides the complex advantage over conventional antibiotics. Available methods of the complex in situ and in vitro biosynthesis make this idea practicable.
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Antibacterianos/farmacología , Péptidos Catiónicos Antimicrobianos/farmacología , Bacterias/efectos de los fármacos , Biopelículas/efectos de los fármacos , Dípteros/fisiología , Farmacorresistencia Bacteriana/efectos de los fármacos , Larva/fisiología , Animales , Biopelículas/crecimiento & desarrollo , Productos Biológicos , Pruebas de Sensibilidad MicrobianaRESUMEN
Antimicrobial peptides accumulated in the hemolymph in response to infection are a key element of insect innate immunity. The involvement of the fat body and hemocytes in the antimicrobial peptide synthesis is widely acknowledged, although release of the peptides present in the hemolymph from the immune cells was not directly verified so far. Here, we studied the presence of antimicrobial peptides in the culture medium of fat body cells and hemocytes isolated from the blue blowfly Calliphora vicina using complex of liquid chromatography, mass spectrometry, and antimicrobial activity assays. Both fat body and hemocytes are shown to synthesize and release to culture medium defensin, cecropin, diptericins, and proline-rich peptides. The spectra of peptide antibiotics released by the fat body and hemocytes partially overlap. Thus, the results suggest that insect fat body and blood cells are capable of releasing mature antimicrobial peptides to the hemolymph. It is notable that the data obtained demonstrate dramatic difference in the functioning of insect antimicrobial peptides and their mammalian counterparts localized into blood cells' phagosomes where they exert their antibacterial activity.
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Péptidos Catiónicos Antimicrobianos/biosíntesis , Péptidos Catiónicos Antimicrobianos/farmacología , Dípteros/citología , Cuerpo Adiposo/citología , Hemocitos/citología , Secuencia de Aminoácidos , Animales , Péptidos Catiónicos Antimicrobianos/química , Células Cultivadas , Fraccionamiento Químico , Cromatografía Líquida de Alta Presión , Medios de Cultivo/farmacología , Larva/citología , Larva/efectos de los fármacos , Linfocitos/citología , Linfocitos/efectos de los fármacos , Peso MolecularRESUMEN
BACKGROUND: Surgical correction of the congenital muscular torticollis (CMT) is recommended for patients with unsuccessful conservative treatment. Open operative techniques all leave noticeable scars. Tenotomy can be performed endoscopically. We proposed the modified endoscopic two-trocar transaxillary approach for the treatment of CMT. MATERIALS AND METHODS: We have applied a technique of endoscopic release of the sternocleidomastoid (SCM) muscle in 5 pediatric patients. We performed the tunnelization of the space over the clavicular and sternal heads of the SCM muscle applied balloon inflation of the Foley catheter (Fr. 16). The sternal and clavicular attachments were dissected and divided by electrocautery hook. RESULTS: An excellent result was found in all 5 patients. There were no complications to be seen. The neurovascular structures were preserved in all cases. CONCLUSIONS: We believe that the subcutaneous endoscopic transaxcillary tenotomy procedure is a good method for the treatment of congenital muscular torticollis. This endoscopic technique avoids injury to the neurovascular structures and does not leave visible scars.
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Endoscopía , Tendones/cirugía , Tortícolis/congénito , Tortícolis/cirugía , Axila , Niño , Preescolar , Femenino , Humanos , Masculino , Resultado del TratamientoRESUMEN
Femtosecond absorption difference spectra were measured for chlorosomes isolated from the green bacterium Chloroflexus aurantiacus at room temperature. Using the relative difference absorption of the oligomeric BChl c and monomeric BChl a bands, the size of a unit BChl c aggregate as well as the exciton coherence size were estimated for the chlorosomal BChl c antenna under study. A quantitative fit of the data was obtained within the framework of the exciton model proposed before [Fetisova et al. (1996) Biophys J 71: 995-1010]. The size of the antenna unit was found to be 24 exciton-coupled BChl c molecules. The anomalously high bleaching value of the oligomeric B740 band with respect to the monomeric B795 band provided the direct evidence for a high degree of exciton delocalization in the chlorosomal B740 BChl c antenna. The effective delocalization size of individual exciton wavefunctions (the thermally averaged inverse participation ratio) in the chlorosomal BChl c antenna is 9.5, whereas the steady-state wavepacket corresponds to the coherence size (the inverse participation ratio of the density matrix) of 7.4 at room temperature.