RESUMEN
Objective: To evaluate the safety of diphtheria, tetanus and acellular pertussis (DTaP) containing combination vaccines used in Chengdu. Methods: The AEFI reports data of DTaP vaccine, DTaP-Haemophilus influenza type b combined vaccine (DTaP-Hib) and DTaP-inactivated poliovirus-Hib combined vaccine (DTaP-IPV-Hib) in Chengdu from 2015 to 2019 were collected through the national immunization management system. Description epidemiological method was used to analyze the data. Results: From 2015 to 2019, a total of 8 234 cases of AEFI of DTaP containing combination vaccines were reported in Chengdu, with a reported incidence of 194.55/100 000 doses, including 7 897 cases of common adverse reaction (168.59 per 100 000) and 234 cases of rare adverse reaction (5.53 per 100 000). The DTaP vaccine reported 4 240 cases AEFI (140.63 per 100 000), the DTaP-Hib vaccine reported 2 490 cases AEFI (399.09 per 100 000) and the DTaP-IPV-Hib vaccine reported 1 504 cases AEFI (253.49 per 100 000). All the three vaccines had the highest incidence for the booster doses; the rare adverse reaction were mainly Anaphylactic Reaction (6.27 per 100 000). Conclusions: The AEFI monitor system had high sensitivity, and the rare adverse reaction rate was extremely low, all the vaccines had good safety profiles. The Thrombocytopenic purpura and Laryngeal Edema should be paid more attention to.
Asunto(s)
Vacunas contra Difteria, Tétanos y Tos Ferina Acelular , Difteria , Vacunas contra Haemophilus , Tétanos , Tos Ferina , Anticuerpos Antibacterianos , Humanos , Lactante , Vacuna Antipolio de Virus Inactivados/efectos adversos , Vacunas Combinadas/efectos adversos , Vacunas Conjugadas/efectos adversosRESUMEN
This study aimed to investigate the absorption mechanism of three curcumin constituents in rat small intestines. Self-emulsification was used to solubilize the three curcumin constituents, and the rat in situ intestinal perfusion method was used to study factors on drug absorption, including drug mass concentration, absorption site, and the different types and concentrations of absorption inhibitors. Within the scope of experimental concentrations, three curcumin constituents were absorbed in rat small intestines through the active transport mechanism.
Asunto(s)
Adyuvantes Farmacéuticos/farmacología , Curcumina/análogos & derivados , Curcumina/farmacocinética , Inhibidores Enzimáticos/farmacocinética , Absorción Intestinal , Intestino Delgado/metabolismo , 2,4-Dinitrofenol/farmacocinética , Transportadoras de Casetes de Unión a ATP/antagonistas & inhibidores , Animales , Cromatografía Líquida de Alta Presión/métodos , Curcumina/química , Diarilheptanoides , Emulsiones , Femenino , Absorción Intestinal/efectos de los fármacos , Intestino Delgado/efectos de los fármacos , Masculino , Proteína 2 Asociada a Resistencia a Múltiples Medicamentos , Proteínas Asociadas a Resistencia a Múltiples Medicamentos/análisis , Proteínas Asociadas a Resistencia a Múltiples Medicamentos/antagonistas & inhibidores , Imagen de Perfusión/métodos , Probenecid/farmacología , Ratas Sprague-Dawley , Valores de Referencia , Reproducibilidad de los Resultados , Factores de Tiempo , Desacopladores/farmacología , Verapamilo/farmacologíaRESUMEN
Glargine is widely used as a long-acting insulin analogue in the treatment of diabetes mellitus. However, this insulin analogue has been recently suspected to be associated with an increased risk of cancer. The aim of this study was to investigate the influence of glargine on proliferation of breast adenocarcinoma cell line (MCF-7) and its possible mechanism. Effects of glargine and regular human insulin on the cell proliferation were tested in ER-positive MCF-7 cells by MTT assay. Apoptosis in MCF-7 cells was measured by flow cytometry. The protein levels of p-AKT, Bcl-2, and Bax were also determined by Western blotting and immunohistochemistry, respectively. The result showed that glargine (100, 200 nmol/l) stimulated proliferation of ER-positive MCF-7 cells compared with regular human insulin. At the same time, glargine decreased the percentage of early apoptosis in MCF-7 cells. Otherwise, glargine (100 nmol/l) stimulated the p-AKT in a time-dependent manner in MCF-7 cells. Furthermore, we found that glargine downregulated the level of Bax protein and upregulated that of Bcl-2 (p <0.05). These data show that glargine promote the proliferation of breast adenocarcinoma cells in vitro, probably by preventing apoptosis.
Asunto(s)
Adenocarcinoma/enzimología , Adenocarcinoma/patología , Neoplasias de la Mama/enzimología , Neoplasias de la Mama/patología , Insulina de Acción Prolongada/farmacología , Proteínas Proto-Oncogénicas c-akt/metabolismo , Apoptosis/efectos de los fármacos , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Regulación hacia Abajo/efectos de los fármacos , Activación Enzimática/efectos de los fármacos , Femenino , Humanos , Insulina Glargina , Fosforilación/efectos de los fármacos , Regulación hacia Arriba/efectos de los fármacos , Proteína X Asociada a bcl-2/metabolismoAsunto(s)
Historia de la Enfermería , Filosofía en Enfermería , Valores Sociales , Historia del Siglo XX , Humanos , TaiwánRESUMEN
Blood selenium concentrations in 111 nasopharyngeal cancer patients and 56 normal subjects were determined using a modified 2,3-diamino naphthalene fluorometric method. Cancer subjects in this study were divided into two groups and blood Se levels (mean +/- SD) were 0.105 +/- 0.024 for untreated cases, 0.113 +/- 0.034 for those who had received treatment, and 0.109 +/- 0.030 microgram/ml for all subjects. The results suggest that low blood Se levels in nasopharyngeal cancer patients are a consequence of their disease rather than the cause of the cancer.