Exploring the anxiolytic mechanism of Fructus gardeniae based on metabolomics, network pharmacology, and molecular docking.

OBJECTIVE: To explore the effect and anxiolytic mechanism of a natural remedy called Fructus gardeniae (FG). METHODS: The elevated-plus maze (EPM) test was used to confirm the anxiolytic effect of FG. The potential and anxiolytic components, targets, and route processes of FG were investigated using the network pharmacology method in conjunction with metabolomics and molecular docking technologies. RESULTS: FG could greatly enhance the proportion of time and times of opening arms, according to the EPM data. As to the metabolomics findings, a total of 61 distinct metabolites were found, mainly involved in glycine, serine, and threonine metabolism as well as alanine, aspartate, and glutamate metabolism. The primary active ingredients of FG, nicotiflorin, jasminodiol, and crocetin, demonstrated substantial binding affinities with monoamine oxidase A (MAOA), monoamine oxidase A (ACHE), malate dehydrogenase 2 (MDH2), glutamate decarboxylase 2 (GAD2), glutamate decarboxylase 1 (GAD1), and nitric oxide synthase (NOS1), according to the findings of network pharmacology and molecular docking. CONCLUSION: FG exerts an anxiolytic action via targeting MAOA, ACHE, MDH2, GAD2, GAD1, and NOS1, and regulating the metabolism of glycine, serine, and threonine as well as alanine, aspartic acid, and glutamic acid.

Pharmacodynamic and targeted amino acid metabolomics researches on the improvement of diabetic retinopathy with Fufang Xueshuantong component compatibility.

The Fufang Xueshuantong capsule (FXT) has significant preventive and therapeutic effects on diabetic retinopathy(DR), but the compatibility of its active components remains to be thoroughly explored. In this study, a zebrafish diabetic retinopathy model was established using high-mixed sugars, and the optimal ratios of notoginseng total saponins, total salvianolic acid, astragaloside, and harpagide were selected through orthogonal experiments. Furthermore, we used UPLC-QqQ/MS to detect the changes in amino acid content of DR zebrafish tissues after administration of FXT and its compatible formula to analyze the effects of FXT and its compatible formula on amino acid metabolites. The results showed that the final compatibility ratios of the components were 8: 5: 1: 6.6 by comprehensive evaluation of the indicators. FXT and its compatibility formula had beneficial effects on retinal vasodilatation, lipid accumulation in the liver, total glucose, and VEGF levels in DR zebrafish, and all of them could call back some amino acid levels in DR zebrafish. In this research, we determined the compatible formulation of the active ingredients in the FXT and investigated their efficacy in DR zebrafish for further clinical applications.

Characteristics of Elliptical Vibration-Assisted Cutting with Variations in Tilt Angle of Elliptical Locus.

Elliptical vibration-assisted cutting (EVAC), one of the advanced micromachining methods, enables results not possible with traditional ultra-precision machining. It is considered to be one of the most viable options for manufacturing micro/nanostructured surfaces. However, it is difficult to control the elliptical locus with different tilt angles; therefore, previous studies have primarily focused on fixed locus and investigated the effects of the amplitude and frequency on machining performance. In addition, tilt angle is an important factor affecting the characteristics of EVAC. To maximize the cutting performance of EVAC, the cutting characteristics of EVAC with variations in tilt angle of elliptical locus are investigated. The mathematical model of elliptical trajectory based on different tilt angles is established via geometric analysis. The effects of the different tilt angle (0-180°) on cutting forces, chip formation, defect generation and surface roughness are observed and theoretically analyzed in microgroove experiments. The experimental results show that the tilt angle has a significant effect on the cutting force, chip formation, defects and surface roughness. The best cutting performance can be obtained at the tilt angle of 30°, while the worst is recorded at 90°. The results can provide a valuable reference for further comprehensive studies to maximize the cutting performance of EVAC.

Fabrication of Large-Area Micro-Hexagonal Cube Corner Retroreflectors on Three-Linear-Axis Ultraprecision Lathes.

Hexagonal cube corner retroreflectors (HCCRs) are the micro-optics arrays with the highest reflectivity. However, these are composed of prismatic micro-cavities with sharp edges, and conventional diamond cutting is considered unmachinable. Besides, 3-linear-axis ultraprecision lathes were considered unfeasible to fabricate HCCRs due to the lack of a rotation axis. Therefore, a new machining method is proposed as a viable option to manufacture HCCRs on the 3-linear-axis ultraprecision lathes in this paper. For the mass production of HCCRs, the dedicated diamond tool is designed and optimized. The toolpaths are proposed and optimized to further increase tool life and machining efficiency. The Diamond Shifting Cutting (DSC) method is analyzed in-depth both theoretically and experimentally. By using the optimized methods, the large-area HCCRs with a structure size of 300 µm covering an area of 10 × 12 mm2 are successfully machined on 3-linear-axis ultraprecision lathes. The experimental results show that the whole array is highly uniform, and the surface roughness Sa of three cube corner facets is all less than 10 nm. More importantly, the machining time is reduced to 19 h, which is far less than the previous processing methods (95 h). This work will significantly reduce the production threshold and costs, which is important to promote the industrial application of HCCRs.

Ultraprecision machining and on-machine measurement of the radial Fresnel lens structure array of a large-scale roller mold.

Automation and on-machine manufacturing process measurement is essential in increasing product productivity. However, a significant challenge is the radial Fresnel lens structure-array machining and on-machine measurement research for a large-scale roller mold. We present a rotating A-axis machining method for manufacturing an array of a radial Fresnel lens structure, based on an ultraprecision four-axis drum roll lathe system developed by the team. Therefore, based on the principle of a virtual multiprobe, the traditional two-point method is improved and applied to the on-machine measurement of the radial Fresnel lens structure of the roll mold. Through the machining and on-machine measurement experiments of the radial Fresnel lens structure of the roller mold, the feasibility of the machining method and measurement method is verified.

Diamond microscraping for the fabrication of a trimmed radial Fresnel array on a roller mold.

Roll-to-roll (R2R) imprinting is a high-throughput and low-cost continuous manufacturing technique for the mass production of high-quality functional optical polymer films. In fabricating optical films, roll molds with high precision and surface finishs are key tooling components in the R2R imprinting process. However, the trimmed radial Fresnel array results in discontinuity of the machining trajectory. Therefore, direct diamond turning of trimmed radial Fresnel array on a roller mold was considered infeasible. We use the diamond microscraping method to manufacture the trimmed radial Fresnel array on the roller mold. The trimmed radial Fresnel array is divided into trimmed and the complete Fresnel structure to be machined separately. The trimmed part adopts the rounded corners method to avoid a damage machined surface. Due to the large size and heavy weight of the roller mold, conventional offline measurement methods cannot be used, so we measure and evaluate its cross-sectional profile by an on-machine measurement method to verify the feasibility of the proposed method. We provide a solution for surface machining of discontinuous and complex microstructures on the roller mold.

Trimetallic AuPtCo Nanopolyhedrons with Peroxidase- and Catalase-Like Catalytic Activity for Glow-Type Chemiluminescence Bioanalysis.

Chemiluminescence (CL) with stable and glowing light emission is vital for the accurate detection of biomarkers. Moreover, the catalyst plays an important role in CL systems. Herein, the trimetallic AuPtCo nanopolyhedrons with peroxidase- and catalase-like catalytic activities are readily synthesized via a one-step reduction method. After reaction with the substrate ABEI and oxidant H2O2, the AuPtCo nanozyme can catalyze the CL emission in a flash type. Interestingly, it has been found that the biofunctionalization of the AuPtCo surface can endow the catalytic interface with a slow-diffusion effect, thereby prolonging the emission of glow-type CL. On this basis, two biofunctionalized AuPtCo nanocomposites, named as AuPtCo@Cys and AuPtCo@Ab, are prepared, achieving sensitive and selective detection of H2O2 and lipoprotein-associated phospholipase A2 (Lp-PLA2), respectively. Further, the proposed glow-type CL assays are successfully applied for the determination of H2O2 and Lp-PLA2 in female vaginal discharge and human serum samples, respectively, which exhibit good correlation with the clinical results. Overall, the trimetallic AuPtCo nanozyme-based glow-type CL analysis has demonstrated as a powerful and robust tool for biomarker analysis, which holds great promise in clinical applications.

Wideband reconfigurable signal generation based on recirculating frequency-shifting using an optoelectronic loop.

A novel photonic-assisted reconfigurable wideband signal generator for linearly frequency-modulated (LFM) signals generation is proposed and experimentally demonstrated. A frequency-shifting recirculating optoelectronic (FSRO) loop is employed to shift and stitch a seed signal repeatedly in the time and the frequency domains through feedback modulation. After experiencing multiple recirculation, a time duration and bandwidth extended LFM signal with a quadratically varying phase of no phase discontinuity is generated. By simply changing loop parameters, the time duration, the bandwidth and the central frequency of the generated LFM signals are adjustable. Although the phase noise will deteriorate during the recirculation, the generated LFM signals with an increased time bandwidth product (TBWP) are still suitable for practical radar applications. A proof-of-concept experiment is carried out. The generation of LFM signals in C and X bands with a TBWP increased by a maximum factor of 196 are achieved. Microwave imaging of rotational targets based on the generated LFM signal is also demonstrated.

Single probe shear scanning method for on-machine measurement of an optical profile.

This study presents a new (to the best of our knowledge) error separation method with a single displacement probe, named as single probe shear scanning (SPSS) method, for the on-machine optical profile measurement to overcome the problems of the existing multiprobe method like the large deviation of probe spacing and the probes' performance difference. The confocal sensor with superior dynamic range, high lateral resolution, and large measurement angle to surface is applied in this study to fulfill the measurement of the optical aspheric surface. The single probe measurement system, in which the probe fixed on a flexure hinge is driven straight within a millimeter-level travel range, is established to realize the function of the multiprobe. For the established system, a new exact profile reconstruction algorithm is built to eliminate the influences of straightness errors of the scanning stage and the systemic errors of shear stage, and to reduce the effect of the sensor drift. The reconstruction algorithms by difference measurement with two shears are studied to build the bidirectional segment stitching reconstruction method, which reduces the error accumulation and improves the reconstruction accuracy under the condition of measuring errors. A profile reconstruction method with three shears measurement is proposed to make a further improvement on the reconstruction accuracy. The proposed reconstruction method with three shears measurement is successfully employed for the on-machine measurement of an aspheric surface profile, and the evaluation results agree well with those from the Taylor profiler.

Novel RF-source-free reconfigurable microwave photonic radar.

A novel reconfigurable microwave photonic (MWP) radar has been proposed and experimentally demonstrated. At a transmitting end, a microwave signal with a large bandwidth and ultra-low phase noise is generated by a Fourier domain mode locking optoelectronic oscillator. At a receiving end, photonics-based de-chirp processing is implemented by phase-modulating light waves in a dual-drive Mach-Zehnder modulator and mixing the modulated light waves at a photodetector. Without the requirement of external RF sources, the developed photonics-assisted programmable radar is capable of generating and processing microwave signals with adjustable format, bandwidth and central frequency. The proposed radar working from X to Ku band with an instantaneous bandwidth of 2 GHz is demonstrated. The reconfiguration of the radar is theoretically analyzed. The tunability of radar bandwidth and central frequency is investigated. Microwave imaging of a pair of trihedral corner reflectors based on the developed MWP radar is achieved.

Sludge disinfection using electrical thermal treatment: The role of ohmic heating.

Electrical heating has been proposed as a potential method for pathogen inactivation in human waste sludge, especially in decentralized wastewater treatment systems. In this study, we investigated the heat production and E. coli inactivation in wastewater sludge using electrical thermal treatment. Various concentrations of NaCl and NH4Cl were tested as electrolyte to enhance conductivity in sludge mixtures. At same voltage input (18V), sludge treated with direct current (DC) exhibited slower ascent of temperature and lower energy efficiencies for heat production comparing to that using alternate current (AC). However, DC power showed better performance in E. coli inactivation due to electrochemical inactivation in addition to thermal inactivation. Greater than 6log10 removal of E. coli was demonstrated within 2h using 0.15M of NaCl as electrolyte by AC or DC power. The heat production in sludge was modeled using Maxwell-Eucken and effective medium theory based on the effective electrical conductivity in the two-phase (liquid and solid) sludge mixtures. The results showed that the water and heat loss is a critical consideration in modeling of sludge temperature using ohmic heating. The experimental data also suggested that the models are less applicable to DC power because the electrochemical reactions triggered by DC reduce the concentration of NH4+ and other ions that serve as electrolyte. The results of this study contribute to the development of engineering strategies for human waste sludge management.

Exact recovery of wavefront from multishearing interferograms in spatial domain.

An exact algorithm based on the multishearing interferograms has been proposed to reconstruct a two-dimensional wavefront. It allows large shears and high resolution of the reconstructed wavefront to be achieved. In this paper, we use simultaneous linear equations to express the relationship between difference wavefronts and the unknown original wavefront, and then the least-squares method is applied to reconstruct the wavefront. To solve the memory problem, an improved wavefront reconstruction algorithm based on virtual subaperture stitching was proposed to improve the calculation efficiency. Lastly, numerical simulations are implemented and the proposed algorithm is compared with another modal and zonal method. The results indicate that the proposed algorithm is capable of reconstructing continuous or discontinuous wavefronts exactly with a large grid. Numerical simulation also shows high accuracy recovery capability of the proposed method in the existence of mixed noise.

Effect of pressure relaxation and membrane backwash on adenovirus removal in a membrane bioreactor.

Pressure relaxation and permeate backwash are two commonly used physical methods for membrane fouling mitigation in membrane bioreactor (MBR) systems. In order to assess the impact of these methods on virus removal by MBRs, experiments were conducted in a bench-scale submerged MBR treating synthetic wastewater. The membranes employed were hollow fibers with the nominal pore size of 0.45 µm. The experimental variables included durations of the filtration (tTMP>0), pressure relaxation (tTMP=0) and backwash (tTMP<0) steps. Both pressure relaxation and permeate backwash led to significant reductions in removal of human adenovirus (HAdV). For the same value of tTMP>0/tTMP=0, longer filtration/relaxation cycles (i.e. larger tTMP+tTMP=0) led to higher transmembrane pressure (TMP) but did not have a significant impact on HAdV removal. A shorter backwash (tTMP<0 = 10 min) at a higher flow rate (Q = 40 mL/min) resulted in more substantial decreases in TMP and HAdV removal than a longer backwash (tTMP<0 = 20 min) at a lower flow rate (Q = 20 mL/min) even though the backwash volume (QtTMP<0) was the same. HAdV removal returned to pre-cleaning levels within 16 h after backwash was applied. Moderate to strong correlations (R(2) = 0.63 to 0.94) were found between TMP and HAdV removal.

Direct polishing of aluminum mirrors with higher quality and accuracy.

Diamond-turned aluminum mirrors have been widely used in modern optical systems. However, the machined surfaces have characteristic periodic tool marks, which contribute to reduced optical performance by creating effects such as scattering and distortion. This paper develops a new polishing technology to polish aluminum mirrors directly to remove tool marks and improve surface quality and surface accuracy. A Taguchi experiment was used to obtain optimal polishing conditions for reducing surface roughness, and computer-controlled optical surfacing technology was employed for form correction of aluminum mirrors.

Exact wavefront recovery with tilt from lateral shear interferograms.

A novel method is presented for one-dimensional wavefront recovery on the basis of difference measurements from two shearing interferograms with varying tilt. The method uses large shears and obtains high lateral resolution. Furthermore, the wavefront under test can be recovered exactly up to an arbitrary constant and straight line at all evaluation points with suitably chosen shears of two shearing interferograms.