Effect of different drying methods on the functional properties of probiotics encapsulated using prebiotic substances.

Probiotics and prebiotics together work synergistically as synbiotics and confer various health benefits. Many studies on synbiotic foods only focus on the survival of probiotics but fail to evaluate their functional properties. The impact on functional properties should be explored to better understand its therapeutic efficacy. In this work, probiotics (Lactiplantibacillus plantarum NCIM 2083) were encapsulated with prebiotics (fructooligosaccharide + whey protein + maltodextrin) using spray-drying (SD), freeze-drying (FD), spray-freeze-drying (SFD), and refractance window-drying (RWD) techniques. Aggregation, intestinal adhesion, antagonistic activity, and bile salt hydrolase (BSH) activity of probiotics were studied before and after the encapsulation process. The SFD probiotics showed better aggregation ability (79% at 24-h incubation), on par with free cells (FC) (81% at 24-h incubation). The co-aggregation ability of encapsulated probiotics has drastic variations with each pathogenic strain. The adhesion ability of probiotics in chicken intestinal mucus was assessed by the crystal violet method, indicating no significant variations between FC and SFD probiotics. Also, encapsulated probiotics exhibit antagonistic activity (zone of inhibition in mm) against gut pathogens E. coli (11.33 to 17.34), S. faecalis (8.83 to 15.32), L. monocytogenes (13.67 to 18), S. boydii (12.17 to 15.5), and S. typhi (2.17 to 6.86). Overall, these studies confirm the significance and impact of various drying techniques on the functionality of encapsulated probiotics in synbiotic powders. KEY POINTS: • Understanding the relevance of processing effects on the functionality of probiotics. • Spray-freeze-dried probiotics showed superior functional properties. • The encapsulation process had no significant impact on bile salt hydrolase activity.

Targeted Delivery of Probiotics: Perspectives on Research and Commercialization.

Considering the significance of the gut microbiota on human health, there has been ever-growing research and commercial interest in various aspects of probiotic functional foods and drugs. A probiotic food requires cautious consideration in terms of strain selection, appropriate process and storage conditions, cell viability and functionality, and effective delivery at the targeted site. To address these challenges, several technologies have been explored and some of them have been adopted for industrial applicability. Encapsulation of probiotics has been recognized as an effective way to stabilize them in their dried form. By conferring a physical barrier to protect them from adverse conditions, the encapsulation approach renders direct benefits on stability, delivery, and functionality. Various techniques have been explored to encapsulate probiotics, but it is noteworthy that the encapsulation method itself influences surface morphology, viability, and survivability of probiotics. This review focuses on the need to encapsulate probiotics, trends in various encapsulation techniques, current research and challenges in targeted delivery, the market status of encapsulated probiotics, and future directions. Specific focus has been given on various in vitro methods that have been explored to better understand their delivery and performance.

Studies on antibacterial and chemotaxis properties of Pseudomonas aeruginosa TEN01 biomass-derived sustainable biosurfactant.

Biosurfactant producing bacterial strains were isolated from oil-contaminated sites at Chennai Petroleum Corporation Limited, Chennai, the potential strain was selected and identified as Pseudomonas aeruginosa TEN01 by 16 S rRNA sequencing technique. Biosurfactant was produced from cassava solid waste from the sago industry. Further, it was extracted by solvent extraction and partially purified by column chromatography. The partially purified biosurfactant was qualitatively analyzed by Thin Layer Chromatography (TLC), quantitatively analyzed by anthrone assay and characterized by Fourier Transform Infra-Red Spectroscopy (FT-IR) and Gas Chromatography-Mass Spectrometry (GC-MS). Rf value and chemical groups confirm the presence of glycolipid in the partially purified biosurfactant. GC-MS results confirmed the presence of long-chain fatty acids and carbohydrate which is found to be mainly present in glycolipids. Biosurfactants are surface-active molecules which have been found to be the best alternative to chemical-based surfactants. The present study focuses on modifying the cell surface using a biosurfactant from P. aeruginosa TEN01 to enhance membrane permeabilization. Antibacterial and chemotaxis properties of biosurfactant from P. aeruginosa TEN01 were found to be better towards Xenorhabdus poinarii, a bio-pesticide producing microbial strain, X. poinarii exhibited 81.7% adhesion to hydrocarbons upon biosurfactant treatment as analyzed by Bacterial Adhesion to Hydrocarbon (BATH) assay. The alteration in the membrane permeability was tested in X. poinarii using biosurfactant and chemical surfactants viz. Sodium dodecyl sulfate (SDS) and toluene by estimating the amount of intracellular protein released. High protein recovery (51.55%) was achieved with a biosurfactant. Cell viability in the biosurfactant-treated cells was also high (93.98%) in comparison to cells treated with chemical surfactants. Increased recovery of intracellular protein along with high cell viability makes the biosurfactant a potential candidate for application in numerous environmental fields.