[Transcriptome and quality evaluation of Sichuan Dao-di herbs Zanthoxylum bungeanum].

"Huajiao" is dried ripe fruit peel of Zanthoxylum bungeanum or Z. schinifolium, is konwn as geoherbs, especially the "Dahongpao" cultivated in Hanyuan, Maoxian and Jiulong of Sichuan province. However, the genetic basis of Dao-di "Huajiao" is virtually unknown. The transcriptome of the fruit and leaf from Sichuan(Hanyuan, Jiulong, Lixian, Maoxian), Gansu(Wudu) province and Shaanxi(Fengxian) province was sequenced. Trinity de novo assembling resulted in a total of 177 616 unigenes. Through the KEGG, NR, SwissProt, Trembl, KOG/COG, GO, Pfam database comparision 106 644 annotated Unigene finally, 4 574 deferentially expressed genes were found in fruit between Sichuan and other provinces, including 3 740 up-regulated genes and 834 down-regulated genes. Among the up-regulated genes, 27 up-regulated genes were raleted to terpenoids, and 8 up-regulated genes were related to isoquinoline alkaloid bio-synthesis. Furthermore, it was also showed remarkable differences in groups which enrichment ratio of the diffe-rent expressed gene compared. The different expressed genes were annotated by the KEGG database into plant-pathogen interaction, plant hormone signal transduction and phenylpropanoid biosynthesis in fruit and leaf, but isoflavonoid bio-synthesis and betaine bio-synthesis were significantly different in fruit and leaf. The study laid a certain reference basis for comparison of quality and different expressed gene of Z. bungeanum from different groups.

[Study on Variety Test of Salvia miltiorrhiza New Variety "Zhongdan 1"].

Objective: The new varieties of Salvia miltiorrhiza were bred by the variety comparison test. Methods: Taking these varieties of CDS1,CDS2,CDS3,CDS4,CDS5 as the test varieties, which bred by system seletion from abundant germplasm resources,the conventional variety as the control, the test were arranged in Ximeishan, Linjiagou, Shiya, Hexing Village of Zhongjiang county in 2010 and 2011. And the new varieties from the growth cycle,line of characters,yield, quality and other aspects of Salvia miltiorrhiza were evaluated. Results: CDS2 had the highest yield which was significantly higher than the control group. The growth period were 250 ~ 270 days,the seed germination were early,and the plants were taller. And the medicine merchandise traits were better, the salvianolic acid and the tanshinone were significantly higher. CDS2 was named as the "Zhongdan 1"by crop variety Committee of Sichuan province in2012,which identified by technology field in 2011. Conclusion: "Zhongdan 1"is a new variety through the certification authority validation,which has a great popularization value in the production.

[Dynamic accumulation regulation of curcumin, demethoxycurcumin and bisdemethoxyeurcumin in three strains of curcuma longae rhizome].

The paper is aimed to study the dynamic accumulation regulation of curcumin (Cur), demethoxycurcumin (DMC) and bisdemethoxyeurcumin (BDMC) in three strains of Curcuma longa, and provide scientific references for formalized cultivation, timely harvesting, quality control and breeding cultivation of C. longa. The accumulation regulation of the three curcumin derivatives was basically the same in rhizome of three strains. The relative contents decreased along with plant development growing, while the accumulation per hectare increased with plant development growing. The accumulation of curcuminoids per hectare could be taken as the assessment standard for the best harvest time of C. longa. A3 was the best strain in terms of Cur and BDMC content.

[Study on variety test of Iris tectorum new variety of CSG1].

OBJECTIVE: Iris tectorum new varieties of CSG1, CSG2, CSG4, CSG5, CSG6, CSG7, CSG8 and CSG9 were bred by system selection from abundant germplasm resources. METHODS: Taking conventional variety as the control, comparison test for these new varieties was arranged in Shuangliu, Zhongjiang, Maoxian, Renshou and Anxian in Sichuan. Plot yields were measured after harvesting, yields per mu were calculated and the data were analyzed with DPS 9.50 software. RESULTS: CSG1 had the highest yield which was significantly higher than the control and other varieties. This new variety had passed through the field identification by Sichuan Provincial Crop Variety Approval Committee in 2013 and would be applied for approval in 2014. CONCLUSION: CSG1 is the first batch of Iris tectorum new variety identified by domestic authority and has great promotion potential.