Effect of flavin adenine dinucleotide (FAD) on Desulfovibrio desulfuricans corrosion of pipeline welded joint.

The impact of Flavin adenine dinucleotide (FAD) on sulfate-reducing bacteria (SRB) corrosion of a pipeline welded joint (WJ) was investigated under anaerobic condition in this paper. The results showed that the thickness of the corrosion product on heat affected zone (HAZ) was lower than that on base metal (BM) and welded zone (WZ), and the FAD addition enhanced the development of the protruding microbial tubercles on the WJ. The local corrosion degrees of the BM and WZ coupons were significantly higher than that of the HAZ coupon. Besides, the FAD addition simultaneously promoted local corrosion of all three zones of the WJ in the SRB inoculated environment, and the promotion role was much more pronounced on the WZ coupons. The selective promotion effect of FAD on SRB corrosion in the WJ was attributed to the special structure of the WZ, the selected SRB attachment and the FAD/FADH2 redox feedback cycle.

Recovery of nonketotic hyperglycaemic hemichorea -hemiballismus due to acute ischemic stroke in the contralateral supplementary motor area: a case report and literature review.

INTRODUCTION: There remains uncertainty about the mechanism and specific location of the relative cortex with nonketotic hyperglycaemic hemichorea-hemiballismus (HC-HB). This paper aims to analyse the relationship between the disappearance of HC-HB and the supplementary motor area (SMA) infarction in a patient who recovered following an acute ischemic stroke. CASE PRESENTATION: An 83-year-old female patient with diabetes mellitus presenting with severe and refractory involuntary movement after hypoglycaemic therapy was referred to an outpatient neurosurgery department for further intervention. Laboratory, magnetic resonance imaging (MRI) and computed tomography (CT) neuroimaging and physical examinations were performed. After a diagnosis of HC-HB was confirmed, the patient received hypoglycaemic therapy and haloperidol; however, there was no significant improvement. Brain MRI T1-weighted images and CT scans showed high signal intensity involving the bilateral putamen nucleus. CT perfusion and CT angiography showed a hypo-perfusion in the SMA of the right hemisphere without significant vascular occlusion. Then, aspirin and clopidogrel were administered, and the patient's left leg presented slight involuntary movement three days later. Interestingly, her involuntary movement disappeared again on the second day after the discontinuation of antiplatelet therapy. She was discharged three days later, and her symptoms did not recur during a follow-up for three months. CONCLUSIONS: The SMA dysfunction caused by the acute infarction could terminate or reset the pathological neural path-way of nonketotic hyperglycaemic HC-HB and contribute to the disappearance of the involuntary movement on the contralateral side. The SMA may be a selective intervention target for patients with refractory nonketotic hyperglycaemic HC-HB.

Low efficiency of cathodic protection in marine tidal corrosion of X80 steel in the presence of Pseudomonas sp.

Owing to the effects of seawater erosion, dry/wet cycles, dissolved oxygen and microorganisms, the corrosion of steel in marine tidal environments is a serious threat to the safe and stable operation of marine equipment and facilities. Among them, microbiologically influenced corrosion (MIC) of steel has received increasing attention. Cathodic protection (CP) is frequently used to control the corrosion of offshore steel structures. However, in the presence of microorganisms, implementation of CP and its specific effects remain controversial. In this study, the influence of Pseudomonas sp. on the CP efficiency of Zn sacrificial anodes (ZnSAs) during the tidal corrosion of X80 steel was studied. The results showed that CP efficiency exceeded 92% in an abiotic tidal environment. However, in the biotic tidal environment, Pseudomonas sp. significantly reduced the CP efficiency. Pseudomonas sp. and its biofilm promoted the corrosion of steel under CP, inhibited the formation of a complete calcareous deposit layer, which weakened the CP efficiency of ZnSA in the marine tidal environment.

Accelerated role of exogenous riboflavin in selective Desulfovibrio desulfuricans corrosion of pipeline welded joints.

Effect of exogenous riboflavin on sulfate-reducing bacteria (SRB) corrosion of a spirally welded joint (WJ) of X80 steel was investigated by SEM/EDS, XPS, 3D ultra-depth microscopy and electrochemical measurements. The main style of SRB corrosion of the WJ is local corrosion. The local corrosion sensitivity of the heating affected zone (HAZ) of the WJ was always lower than that of the weld zone (WZ) and base metal (BM) in all the SRB-inoculated mediums. SRB corrosion of the WJ is selective. With the dosage increase of riboflavin, the selective pitting corrosion of the WJ becomes more pronounced.

Effect of Pseudomonas sp. on simulated tidal corrosion of X80 pipeline steel.

Microbiologically influenced corrosion of pipeline steel in seawater has long been concerned by scholars all over the world, but there were few reports on the microorganism effect on marine tidal corrosion of steels. In this work, the effect of Pseudomonas sp. on static tidal corrosion of X80 pipeline steel were systematically studied using weight-loss, Fourier transform infrared spectroscopy (FTIR), electrochemical measurements, scanning electron microscopy (SEM) and ultra-deep field 3D microscope. The results manifested that after 720 h exposure to the marine tidal environment, the sessile Pseudomonas sp. counts multiplied with the elevation increase. The corrosion style of the steel in the inoculated environment was mainly localized corrosion. As a consequence of the higher bacteria number, the corrosion rate, pit depth and corrosion product thickness collectively enhanced. Pseudomonas sp. significantly accelerated uniform and localized corrosion of the steel in the marine tidal zone, and the acceleration role enhanced with the steel elevation in the tidal zones.

Overexpression of microRNA-96 is associated with poor prognosis and promotes proliferation, migration and invasion in cholangiocarcinoma cells via MTSS1.

MicroRNA-96 (miR-96) has been revealed serve an oncogenic role in various types of cancer. However, the role of miR-96 in cholangiocarcinoma (CCA) development and progression is yet to be elucidated. Thus, the aim of the present study was to investigate the role of miR-96 in CCA. The expression pattern of miR-96 in CCA tissues and cell lines was evaluated using reverse transcription-quantitative PCR analysis. Kaplan-Meier curves and Cox regression analyses were conducted to investigate the prognostic significance of miR-96 in CCA. Cell Counting Kit-8 and Transwell assays were performed to identify the functions of miR-96. The association between miR-96 and metastasis suppressor-1 (MTSS1) was verified using a dual-luciferase assay. The results demonstrated that miR-96 expression levels were increased in CCA tissues and cell lines compared with those in adjacent normal tissues and normal human intrahepatic biliary epithelial cell lines, respectively. High expression levels of miR-96 were significantly associated with lymph node metastasis, differentiation and TNM stage. In addition, upregulated expression of miR-96 was associated with a poorer prognosis and was predicted to be a prognostic factor in patients with CCA. Overexpression of miR-96 in vitro promoted CCA cell proliferation, migration and invasion. Additionally, MTSS1 was identified as a direct target of miR-96. The results of the present study indicated the clinical and biological importance of miR-96 as an oncogene in CCA. miR-96 may represent an independent prognostic biomarker and may promote CCA cell proliferation, migration and invasion by targeting MTSS1.

Chelator-Free Conjugation of 99mTc and Gd3+ to PEGylated Nanographene Oxide for Dual-Modality SPECT/MR Imaging of Lymph Nodes.

PEGylated ultrasmall nanographene oxide (usNGO-PEG) has exhibited a great potential in nanotheranostics due to its newly discovered physicochemical properties derived from the rich functional groups and bonds. Herein, we developed a general, simple, and kitlike preparation approach for 99mTc- and Gd-anchored NGO-PEG using a chelator-free strategy. In this strategy, [99mTcI(CO)3(OH2)3]+ (abbreviated to 99mTcI) and GdCl3 were mixed with usNGO-PEG to yield 99mTc- and Gd-usNGO-PEG via the synergistic coordination of N and O atoms from NGO and PEG with 99mTcI and Gd3+ without additional exogenous chelators. Under optimized conditions, the nanoprobes 99mTc- and Gd-usNGO-PEG were reliably prepared with high yields and good stability. Serial comparative experiments of the labeling yield, the measurements of -NH2 density and ζ-potentials, and various characterizations including energy-dispersive X-ray analysis spectroscopy, X-ray photoelectron spectroscopy, and Fourier-transform infrared spectroscopy demonstrated that both usNGO and PEG synergistically provide the electron-donating atoms O and N to coordinate with 99mTcI and Gd to form stable nanocomplexes. Furthermore, both 99mTc- and Gd-usNGO-PEG exhibited excellent in vivo imaging of lymph nodes using single-photon emission computed tomography/computed tomography (SPECT/CT) and magnetic resonance (MR) imaging after local injection. Therefore, these results showed the successful establishment of 99mTc- and Gd-anchored usNGO-PEG using a chelator-free strategy and the potential of multimodality SPECT/CT and MR imaging of lymph nodes.

Coordination polymer hybridized Au nanocages: a nanoplatform for dual-modality imaging guided near-infrared driven photothermal therapy in vivo.

Coordination polymer hybridized Au nanocages (AuNC@CPs) were prepared via a dialysis process by combining Au nanocages with a coordination polymer, which was derived from (pq)2Ir(Hdcbpy) and Dy(OOCCH3)3. After hybridization, the localized surface plasmon resonance (LSPR) peak of the AuNC@CPs exhibited an evident red shift in comparison with that of the Au nanocages, which was due to a variation in the dielectric constant caused by the coordination polymer. Notably, inspired by their excellent photothermal, photoacoustic, and magnetic properties in solution, AuNC@CPs were used for near-infrared (NIR)-driven photothermal therapy (PTT) guided by photoacoustic (PA) and magnetic resonance (MR) imaging in vivo. Owing to their remarkable performance, the AuNC@CPs would represent promising nanoprobes for theranostics.

99mTc-conjugated manganese-based mesoporous silica nanoparticles for SPECT, pH-responsive MRI and anti-cancer drug delivery.

In recent decades, hybrid imaging techniques that exploit the advantages of multiple imaging technologies have aroused extensive attention due to the deficiencies of single imaging modes. Along with the development of single photon emission computed tomography-magnetic resonance imaging (SPECT-MRI), it is currently necessary to develop a series of dual probes that can combine the outstanding sensitivity of SPECT with the high spatial resolution of MRI. Herein, the commonly used technetium-99 (99mTc) was labelled on the surface of manganese oxide-based mesoporous silica nanoparticles (MnOx-MSNs) for use in SPECT-MRI dual-modal imaging. The radiolabelling yield was as high as 99.1 ± 0.6%, and the r1 value of the nanoprobes was able to reach 6.60 mM-1 s-1 due to the pH-responsive properties of the MnOx-MSNs. The high-performance SPECT-MRI dual-modal imaging was confirmed in vivo in tumour-bearing mice, which could also provide semi-quantitative information for tumour detection. Importantly, these nanoprobes can deliver anti-cancer drugs in cancer therapy due to their unique mesoporous structures. Thus, nanotheranostics combining dual-modal imaging with anti-cancer therapeutic properties were achieved.

Visualization of size-dependent tumour retention of PEGylated nanographene oxide via SPECT imaging.

PEGylated nanosized graphene oxides (NGO-PEG) and related derivatives have attracted extensive attention owing to their unique properties, which confer significant theranostic benefits for cancer treatment. The size of NGO-PEG varies largely, from tens of nanometers to micrometers, and the optimal size range with the most efficient tumor retention in vivo remains to be determined. For this purpose, we designed different sizes of NGO-PEG, specifically, ultra-small NGO-PEG (usNGO-PEG, sub-50 nm) and NGO-PEG (over 50 nm) and compared their biological behaviors in vitro and in vivo. Both NGO-PEGs exhibited nearly identical physicochemical properties and low cytotoxicity. Following Cy5.5 tagging, confocal microscopy fluorescence imaging revealed faster and higher dynamic cellular uptake of usNGO-PEG than NGO-PEG. Longitudinal, non-invasive visualization of the NGO-PEGs using single proton emission computed tomography (SPECT) imaging with 125I-radiolabeling revealed that tumor retention of usNGO-PEG was significantly higher and longer compared to that of NGO-PEG, whereas the blood circulation and biodistribution of both NGO-PEGs were similar in major organs. In conclusion, Sub-50 nm was further confirmed to be the favorable size for efficient tumor accumulation of PEGylated GO via the enhanced permeability and retention (EPR) effect. We propose that the sub-50 nm NGO-PEG designed in this study may be effectively utilized to develop novel PEGylated GO-based nanoplatforms for multifunctional cancer nanotheranostics.

[Observation of the inflammatory factors level and the correlation between induced sputum and whole lung lavage fluid for silicosis].

OBJECTIVE: To detect the inflammatory factors of induced sputum (IS) and whole lung lavage fluid in pneumonoconiosis patients and to explore the correlation between the inflammatory factors with pulmonary function. METHODS: The records of 45 cases of pneumonoconiosis patients were observed. All patients underwent lung function examination, sputum induction and massive whole lung lavage (WLL) sequentially through advance. IS and whole lung lavage fluid were collected respectively. Inflammatory factors of the two specimens were detected by using enzyme linked immunosorbent assay. The correlation of inflammatory factors between the two specimens was analyzed. The relationship between the inflammatory factor and lung function index was observed. The statistical analysis is performed with SPSS 17.0 for Windows. P < 0.05 is considered to be statistically significant. RESULTS: Cytokines (MCP-1, TNF-α MIP-1α, NO(2)(-)/NO(3)(-) and IL-16) were significantly associated between IS and whole lung lavage fluid (P < 0.05), while TNF-α, MCP-1, NO(2)(-)/NO(3)(-) and IL-16 were no significantly associated with lung function index (P > 0.05). MIP-1α was significantly associated with FEV(1.0)/VCmax and MEF(25), respectively (P < 0.05). CONCLUSIONS: Inflammatory factors were significantly associated between IS and whole lung lavage fluid, which could indicate early lung injury in pneumonoconiosis patients.

[Changes of cell factor in bronchoalveolar lavage fluid in rats exposed to silica].

OBJECTIVE: To investigate the changes in the levels of inflammatory cytokines in bronchoalveolar lavage fluid (BALF) in rats exposed to silica dust. METHODS: Experimental rats were randomly divided into control group and three experimental groups (doses of dust: 15, 30, and 60 mg/ml), with 42 rats in each group. Each rat in the control group was treated with 1 ml of normal saline by intratracheal instillation, while each rat in the experimental groups was exposed to 1 ml of silica suspension by a single intratracheal instillation. Seven rats in each group were killed at 1, 3, 7, 14, 21, and 28 days after exposure, and then BALF was collected. Enzyme-linked immunosorbent assay was used to measure the levels of interleukin (IL)-1, IL-6, IL-16, macrophage inflammatory protein-1 alpha (MIP-1α), monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor-α (TNF-α), and transforming growth factor-ß (TGF-ß). RESULTS: The levels of cytokines in each experimental group were higher than those in the control group at any time point. In the early stage of exposure (day 1-3), BALF IL-1 level increased significantly with the increase in dust dose, and on day 14, BALF IL-6 and IL-16 levels increased significantly with the increase in dust dose; the levels of IL-1, IL-6, and IL-16 in the experimental groups reached the peak on day 14. There were significant differences in the levels of MIP-1α and MCP-1 between the experimental groups (FMIP-1α = 30.106, P<0.01; FMCP-1 = 17.193, P<0.01). In each group, the level of MCP-1 varied significantly at different time points (F = 0.618, P>0.05). On day 1-14, BALF TNF-α level increased with the increase in dust dose, with a significant dose-response relationship (P < 0.05). In each experimental group, TNF-α level reached the peak on day 14. On days 14, 21, and 28, the high-dose group had significantly higher BALF TGF-ß levels than the low-dose group (P<0.05); on days 14 and 28, the high-dose group had significantly higher BALF TGF-ß levels than the middle-dose group (P<0.05). CONCLUSION: IL-1, IL-6, IL-16, MIP-1α, MCP-1, and TNF-α play a role in the development and progression of silicosis inflammation. TGF-ß may be related to (related to; associated with; correlated with) fibrosis.