[Effects of intubating laryngeal mask airway in prevention of extubation responses in patients with hypertensive cerebral hemorrhage during general anesthesia recovery period].

OBJECTIVE: To evaluate the effects of intubating laryngeal mask airway (ILMA) in prediction of extubation responses in patients with hypertensive cerebral hemorrhage during general anesthesia recovery period. METHODS: A total of 120 patients with hypertensive cerebral hemorrhage aged 47-71 years, with Glasgow Coma Scale (GCS)11-15 scores and American Society of Anesthesiologists (ASA) physical status Ⅰ or Ⅱ, undergoing craniotomy surgery from December 2012 to December 2014 in the Affiliated First Municipal Hospital of Fujian Medical University were randomly divided into 2 groups (n=60): tracheal intubation (Group T) and intubating laryngeal mask airway (Group I), by using a random number table. Variations of invasive arterial blood pressure and responses of endotracheal extubation were compared between two groups before and after extubation. RESULTS: There were no significant differences in ages, sex ratio, preoperative GCS, operation time and the time from the end of operation to Train of Four stimulation (TOF) becoming to 4 between the two groups. The mean arterial pressure (MAP) at the time point of extubation (T3), 1 min (T4), 3 min (T5), 5 min (T6) after extubation were (136±20), (130±16), (128±12), (125±10) mmHg in Group T, and heart rate(HR) at these four time points were (105±11), (96±8), (92±7), (86±6) bpm, respectively. While in group I, MAP were (108±10), (106±8), (105±9), (106±7) mmHg, and HR were (75±8), (76±7), (68±5), (67±6) bpm, respectively. MAP and HR of the two groups at these four time points had significant differences (T3: t=10.91, 17.20; T4: t=13.72, 14.69; T5: t=12.54, 13.35; T6: t=13.39, 11.27; all P<0.01). During recovery, the incidences of extubation responses of group T and group I were 92% and 2%, respectively, and the difference had statistically significant (χ(2)=94.05, P<0.01). The incidences of coughing of group T and group I were 97% and 0, respectively, and the difference had statistically significant (χ(2)=148.69, P<0.01). CONCLUSION: Compared with tracheal intubation, ILMA can decrease the incidence of extubation responses and coughing in patients with hypertensive cerebral hemorrhage during general anesthesia recovery period, and avoid cere-brovascular accidents.

Isolation and identification of new pollen-specific SFB genes in Japanese apricot (Prunus mume).

SFB, a candidate gene for the pollen S gene, has been identified in several species of Prunus (Rosaceae). We isolated 5 new SFB alleles from 6 Japanese apricot (Prunus mume) lines using a specific Prunus SFB primer pair (SFB-C1F and Pm-Vb), which was designed from conserved regions of Prunus SFB. The nucleotide sequences of these SFB genes were submitted to the GenBank database. The 5 new SFB alleles share typical structural features with SFB alleles from other Prunus species and were found to be polymorphic, with 67.08 to 96.91% amino acid identity. These new SFB alleles were specifically expressed in the pollen. We conclude that the PmSFB alleles that we identified are the pollen S determinants of Japanese apricot; they have potential as a tool for studies of the mechanisms of pollen self-incompatibility.

Differential expression of proteins associated with seasonal bud dormancy at four critical stages in Japanese apricot.

Dormancy is of great significance in the growth and development of deciduous fruit trees. We used a combination of two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionisation time of flight/time of flight mass spectrometry (MALDI-TOF/TOF MS) to identify the differentially expressed proteome of Japanese apricot flower buds at four critical stages, from paradormancy before leaf fall to dormancy release. More than 400 highly reproducible protein spots (P < 0.05) were detected: 34 protein spots showed a greater than twofold difference in expression values, of which 32 protein spots were confidently identified from databases. Identified proteins were classified into six functional categories: stress response and defence (11), energy metabolism (ten), protein metabolism (five), cell structure (three), transcription (one) and unclassified (two). The glyoxalase I homologue could help Japanese apricot survival under various abiotic and biotic stresses, greatly contributing to its dormancy. Enolase, thioredoxin family proteins and triose phosphate isomerase provide adequate energy to complete consecutive dormancy release and bud break in Japanese apricot. Cinnamyl alcohol dehydrogenase 9 and arginase enhance the resilience of plants, enabling them to complete dormancy safely. Analysis of functions of identified proteins and related metabolic pathways will increase our knowledge of dormancy in woody plants.