High-speed performance evaluation of ultra-flexible polymer waveguides supporting meter-scale optical interconnects.

We demonstrate bandwidth measurement and high-speed data transmission of meter-scale connectorized ultra-flexible multimode waveguide links with a maximum length of 180 cm. The pulses propagating through the waveguides broadened linearly with the increase of the length from 20 cm to 240 cm and the estimated mode delay from the pulse broadening was 0.093 ps/cm. The corresponding waveguide bandwidth decreased inversely with the increase of waveguide length, leading to a bandwidth-length product of 42 GHz·m. Degradation in bandwidth due to the introduction of bending or twisting was small when the samples were bent with a bending radius as small as 1 mm for 3 turns or twisted for 4 full turns, respectively. Error-free transmission of 30 Gb/s non-return-to-zero (NRZ) signal was achieved with a record link length up to 140 cm to the best of our knowledge. Our results show that the demonstrated flexible waveguides have both excellent optical and mechanical properties and are ideal for high-speed optical interconnects application especially those have a strict requirement on flexibility.

Direct bandwidth measurement of multimode waveguides based on an optical sampling technique.

We demonstrate direct bandwidth measurement of 11-cm-long multimode polymer waveguides based on an optical sampling technique for the first time, to our knowledge. The pulse shape can be recovered after transmission due to the advantages such as high bandwidth and high refresh rate of optical sampling technology. A reduction in averaged bandwidth (bandwidth-length product) from 241 GHz (27 GHz·m) to 180 GHz (20 GHz·m) of straight waveguides is observed when using mode scramblers to fully stimulate the higher-order modes. The effects such as bending and crossing structure of the waveguides on the bandwidth are also investigated. The proposed method is effective for measuring the bandwidth and dispersion of meter- and centimeter-long waveguides, fibers, and optical devices.

Investigation on roughness-induced scattering loss of small-core polymer waveguides for single-mode optical interconnect applications.

We investigated the roughness-induced scattering loss (LossR) of small-core polymer waveguides fabricated using the photolithography method, both theoretically and experimentally. The dependence of LossR on the roughness parameter, waveguide dimension, operation wavelength, refractive index difference and distribution, polarization sensitivity, sidewall angle, and bending radius were studied. The surface roughness of both the sidewall and the top/bottom of the fabricated waveguides were measured using laser confocal microscope, and the results showed that the averaged sidewall roughness was approximately 60 nm, which is 3 times that of the top/bottom surface. As a result, the sidewall roughness-induced LossR is 9 times that induced by the top/bottom roughness. The calculated value of LossR agrees well with the measured value. LossR increases rapidly with the decrease in the waveguide width, especially when the waveguide width is reduced below 10 µm, at which the LossR is approximately 0.3 dB/cm. On the other hand, the dependence of LossR on the waveguide height is negligible. Our results provide guidance for developing single-mode polymer waveguides with low loss for optical interconnect applications.

[Expression, purification and immunogenicity analysis of HPV type 18 virus-like particles from Escherichia coli].

Here, we presented a method to bacterially express the major structural protein L1 of Human Papillomavirus type 18 (HPV18) as soluble form. We found that the purified L1 could self-assemble to virus-like particles (VLPs). Further, we investigated the immunogenicity and the induced level of neutralizing antibody using these VLPs. First, the genome of HPV18 was cloned from a patient in Xiamen. It was used as template for PCR amplification of HPV18 L1 gene. The resultant DNA fragment was inserted into expression vector pTrxFus and expressed in Escherichia coli GI724. Second, L1 protein was purified by ammonium sulfate precipitation, ion-exchange chromatography and hydrophobic interaction chromatography; and the purified L1 was subjected to self-assembly to form VLPs with the removal of premixed reductant DTT. Finally, the size and morphology of these VLPs was investigated by Dynamic Light Scattering and Transmission Electronic Microscopy as 29.34 nm in hydrated radius and globular particles similar with native HPV18. The half effective dosage (ED50) and maximum level of neutralizing antibody elicitation were measured by vaccinations on mice, rabbit and goat using pseudovirus neutralization cell model. The results showed that the ED50 of HPV18 VLPs is 0.006 microg in mice, and the maximum titer of neutralizing antibody elicited in rabbit and goat is up to 10(7). As a conclusion, we can provide HPV18 VLPs with highly immunogenicity from prokaryote expression system, which may pave a new way for research and development of prophylactic vaccine for HPV18.

[Expression, purification and immunogenicity of human papillomavirus type 11 virus-like particles from Escherichia coli].

OBJECTIVE: To produce human papillomavirus type 11 virus-like particles (HPV11 VLPs) from Escherichia coli and to investigate its immunogenicity and type cross neutralization nature. METHODS: We expressed the major capsid protein of HPV11 (HPV11-L1) in Escherichia coli ER2566 in non fusion fashion and purified by amino sulfate precipitation, ion-exchange chromatography and hydrophobic interaction chromatography, sequentially. Then we removed the reductant DTT to have the purified HPV11-L1 self-assemble into VLPs in vitro. We investigated the morphology of these VLPs with dynamic light scattering and transmission electron microscopy. We assayed the immunogenicity of the resultant HPV11 VLPs by vaccinations on mice and evaluated by HPV6/11/16/18 pseudovirion neutralization cell models. RESULTS: We expressed HPV11 L1 in Escherichia coli with two forms, soluble and inclusion body. The soluble HPV11 L1 with over 95% purity can self assemble to VLPs in high efficiency. Morphologically, these VLPs were globular, homogeneous and with a diameter of - 50 nm, which is quite similar with native HPV11 virions. The half effective dosage (ED50) of HPV11 VLPs is 0.031 microg, and the maximum titer of neutralizing antibody elicited is averaged to 10(6). The cross neutralization activity (against HPV6/16/18) of the anti-HPV11 serum was found to have exact correlation to the inter-type homology in amino acid alignment. CONCLUSION: We can provide HPV11 VLPs with highly immunogenicity from prokaryote expression system, which may pave a new way for research and development of prophylactic vaccine for HPV11.