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1.
Microbiol Resour Announc ; 13(7): e0015824, 2024 Jul 18.
Artículo en Inglés | MEDLINE | ID: mdl-38860813

RESUMEN

The whole genome sequence of a low pathogenicity avian influenza virus (H6N2) was sequenced from a Brazilian teal (Amazonetta brasiliensis) in Brazil, 2023. Phylogenetic analysis of the whole genome revealed a distinct genome pertaining to South American LPAIV from 2014 to 2016, indicating extensive circulation among South American wild birds.

2.
PLoS One ; 19(5): e0300862, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38739614

RESUMEN

Influenza A viruses of the H2 subtype represent a zoonotic and pandemic threat to humans due to a lack of widespread specific immunity. Although A(H2) viruses that circulate in wild bird reservoirs are distinct from the 1957 pandemic A(H2N2) viruses, there is concern that they could impact animal and public health. There is limited information on AIVs in Latin America, and next to nothing about H2 subtypes in Brazil. In the present study, we report the occurrence and genomic sequences of two influenza A viruses isolated from wild-caught white-rumped sandpipers (Calidris fuscicollis). One virus, identified as A(H2N1), was isolated from a bird captured in Restinga de Jurubatiba National Park (PNRJ, Rio de Janeiro), while the other, identified as A(H2N2), was isolated from a bird captured in Lagoa do Peixe National Park (PNLP, Rio Grande do Sul). DNA sequencing and phylogenetic analysis of the obtained sequences revealed that each virus belonged to distinct subtypes. Furthermore, the phylogenetic analysis indicated that the genomic sequence of the A(H2N1) virus isolated from PNRJ was most closely related to other A(H2N1) viruses isolated from North American birds. On the other hand, the A(H2N2) virus genome recovered from the PNLP-captured bird exhibited a more diverse origin, with some sequences closely related to viruses from Iceland and North America, and others showing similarity to virus sequences recovered from birds in South America. Viral genes of diverse origins were identified in one of the viruses, indicating local reassortment. This suggests that the extreme South of Brazil may serve as an environment conducive to reassortment between avian influenza virus lineages from North and South America, potentially contributing to an increase in overall viral diversity.


Asunto(s)
Charadriiformes , Virus de la Influenza A , Gripe Aviar , Filogenia , Virus Reordenados , Animales , Brasil , Gripe Aviar/virología , Gripe Aviar/epidemiología , Virus de la Influenza A/genética , Virus de la Influenza A/aislamiento & purificación , Virus Reordenados/genética , Virus Reordenados/aislamiento & purificación , Charadriiformes/virología , Genoma Viral , Aves/virología
3.
Emerg Infect Dis ; 30(3): 619-621, 2024 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-38290826

RESUMEN

We report 4 highly pathogenic avian influenza A(H5N1) clade 2.3.4.4.b viruses in samples collected during June 2023 from Royal terns and Cabot's terns in Brazil. Phylodynamic analysis revealed viral movement from Peru to Brazil, indicating a concerning spread of this clade along the Atlantic Americas migratory bird flyway.


Asunto(s)
Charadriiformes , Subtipo H5N1 del Virus de la Influenza A , Gripe Aviar , Gripe Humana , Animales , Humanos , Gripe Aviar/epidemiología , Animales Salvajes , Brasil/epidemiología , Aves , Filogenia
4.
Braz J Microbiol ; 54(4): 3221-3230, 2023 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-37653362

RESUMEN

Bats can harbor a diversity of viruses, such as adenovirus. Ten different species of bat adenoviruses (BtAdV A to J) have been previous described worlwide. In Brazil, BtAdV was described in three species of phyllostomid species: Artibeus lituratus, Desmodus rotundus, and Sturnira lilium. There are around 180 bat species in Brazil, with 67% inhabiting the Atlantic Forest, with few information about the circulation of BtAdV in this biome. We aimed to describe the molecular detection and the phylogenetic characterization and suggest a classification of BtAdVs circulating in bats from the Brazilian Atlantic Forest. We collected 382 oral and rectal swabs from 208 bats between 2014-2015 and 2020-2021 from São Paulo, Pernambuco, and Santa Catarina Brazilian states. The adenovirus detection was done by a nested PCR targeting the DNA polymerase gene, and all positive samples were sequenced by the Sanger method. The phylogenetic analyses were based on the amino acid sequences using the MEGA 7 and BEAST software. We obtained 16 positive animals (detection rate 7.7%) belonging to seven bat species: Artibeus lituratus, Carollia perspicillata, Sturnira lilium, Molossus molossus, and the first record of Phyllostomus discolor, Eptesicus diminutus, and Myotis riparius. The phylogenetic analysis based on partial amino acid sequences showed that all obtained AdV sequences belong to the Mastadenovirus genus. We observed a high genetic diversity of BtAdV and identified eleven potential BtAdV species circulating in Brazil (BtAdV K to U). Our results contribute to the epidemiological surveillance of adenovirus, increasing the knowledge about the viral diversity and the distribution of AdV in bats from the Atlantic Forest.


Asunto(s)
Infecciones por Adenoviridae , Quirópteros , Mastadenovirus , Animales , Adenoviridae/genética , Brasil , Filogenia , Variación Genética
5.
Transbound Emerg Dis ; 69(6): e3436-e3446, 2022 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-36217218

RESUMEN

Wild aquatic birds are the natural reservoirs of avian influenza viruses (AIVs). It is estimated that 100 million seabirds live in the Antarctic Peninsula and adjacent islands, regularly encountering migratory birds that use the islands to nest. Between 2010 and 2013, we collected samples from 865 seabirds in Elephant, King George and Livingston islands, around Antarctica Peninsula: chinstrap penguin (n = 143); gentoo penguin (n = 208); Adelie penguin (n = 46); brown skua (n = 90); Cape petrel (n = 115) and southern giant petrel (n = 263). Serum (n = 673) samples were analysed by competitive ELISA and swabs (n = 614) were tested by one step real-time RT-PCR for avian influenza virus (AIV). Sera from 30 chinstrap penguins, 76 brown skuas and a single Adelie penguin were seropositive for AIV. Thirteen swab samples were AIV positive by RT-PCR, and complete genome sequences of H6N8 AIVs isolated from brown skua and chinstrap penguin in 2011 were obtained. Phylogenetic analyses indicated that all gene segments of the H6N8 viruses were closely related to Argentinian and Chilean AIVs. The prevalence with which we identified evidence for AIVs infection in various Antarctic seabirds suggest viral circulation in Antarctic avifauna and interspecies viral transmission in the sub-Antarctic region.


Asunto(s)
Charadriiformes , Virus de la Influenza A , Gripe Aviar , Spheniscidae , Animales , Regiones Antárticas , Gripe Aviar/epidemiología , Filogenia , Animales Salvajes , Virus de la Influenza A/genética , Chile
6.
Transbound Emerg Dis ; 69(5): e2863-e2875, 2022 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-35729863

RESUMEN

Bat coronaviruses (Bat-CoVs) represent around 35% of all virus genomes described in bats. Brazil has one of the highest mammal species diversity, with 181 species of bats described so far. However, few Bat-CoV surveillance programmes were carried out in the country. Thus, our aim was to jevaluate the Bat-CoV diversity in the Atlantic Forest, the second biome with the highest number of bat species in Brazil. We analysed 456 oral and rectal swabs and 22 tissue samples from Atlantic Forest bats, detecting Alphacoronavirus in 44 swab samples (9.6%) targeting the RdRp gene from seven different bat species, three of which have never been described as Bat-CoV hosts. Phylogenetic analysis of the amino acid (aa) sequences coding the RdRp gene grouped the sequences obtained in our study with Bat-CoV previously detected in identical or congeneric bat species, belonging to four subgenera, with high aa identity (over 90%). The RdRp gene was also detected in three tissue samples from Diphylla ecaudata and Sturnira lilium, and the partial S gene was successfully sequenced in five tissues and swab samples of D. ecaudata. The phylogenetic analysis based on the partial S gene obtained here grouped the sequence of D. ecaudata with CoV from Desmodus rotundus previously detected in Peru and Brazil, belonging to the Amalacovirus subgenus, with aa identity ranging from 73.6% to 88.8%. Our data reinforce the wide distribution of Coronaviruses in bats from Brazil and the novelty of three bats species as Bat-CoV hosts and the co-circulation of four Alphacoronavirus subgenera in Brazil.


Asunto(s)
Alphacoronavirus , Quirópteros , Infecciones por Coronavirus , Coronavirus , Alphacoronavirus/genética , Aminoácidos/genética , Animales , Brasil/epidemiología , Coronavirus/genética , Infecciones por Coronavirus/veterinaria , Bosques , Variación Genética , Genoma Viral , Filogenia , ARN Polimerasa Dependiente del ARN
7.
Infect Genet Evol ; 95: 105041, 2021 11.
Artículo en Inglés | MEDLINE | ID: mdl-34411742

RESUMEN

Paramyxoviruses have a broad host range and geographic distribution, including human pathogens transmitted by bats, such as Nipah and Hendra viruses. In this study, we combined high-throughput sequencing and molecular approaches to investigate the presence of paramyxoviruses in neotropical bats (Microchiroptera suborder) in Brazil. We discovered and characterized three novel paramyxoviruses in the kidney tissues of apparently healthy common vampire bats (D. rotundus) and Seba's short-tailed bats (C. perspicillata), which we tentatively named Kanhgág virus (KANV), Boe virus (BOEV), and Guató virus (GUATV). In this study, we classified these viruses as putative species into the Macrojêvirus genus, a newly proposed genus of the Orthoparamyxovirinae subfamily. Using RT-PCR, we detected these viruses in 20.9% (9 out of 43) of bats tested, and viral RNA was detected exclusively in kidney tissues. Attempts to isolate infectious virus were successful for KANV and GUATV. Our results expand the viral diversity, host range, and geographical distribution of the paramyxoviruses.


Asunto(s)
Quirópteros , Infecciones por Paramyxoviridae/veterinaria , Paramyxoviridae/clasificación , Animales , Brasil/epidemiología , Especificidad del Huésped , Paramyxoviridae/fisiología , Filogenia , Prevalencia , ARN Viral/análisis
8.
J Wildl Dis ; 56(3): 631-635, 2020 07.
Artículo en Inglés | MEDLINE | ID: mdl-31917630

RESUMEN

Reticuloendotheliosis viruses (REVs) are known to cause immunosuppressive and oncogenic disease that affects numerous avian species. Reticuloendotheliosis viruses are present worldwide and recently have been reported in South America with cases of infected commercial flocks in Argentina. We surveyed for the presence of REV in birds from a state in the northern region of Brazil using real-time PCR. We report here the presence of REV in Brazil, detected in Muscovy Ducks (Cairina moschata), Wild Turkeys (Meleagris gallopavo), and chickens (Gallus gallus) at a relatively high prevalence (16.8%). Phylogenetic analysis indicated a close relationship of these strains to variants in the US. This study provides evidence of REV in the Amazon biome and provides a baseline for future surveillance of the virus in the region and throughout Brazil.


Asunto(s)
Pollos , Patos , Virus de la Reticuloendoteliosis Aviar/aislamiento & purificación , Reticuloendoteliosis Aviar/virología , Pavos , Animales , Brasil/epidemiología , Variación Genética , Filogenia , Virus de la Reticuloendoteliosis Aviar/genética , Reticuloendoteliosis Aviar/epidemiología
9.
Virus Evol ; 5(2): vez047, 2019 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-31850147

RESUMEN

Picornaviridae family comprises single-stranded, positive-sense RNA viruses distributed into forty-seven genera. Picornaviruses have a broad host range and geographic distribution in all continents. In this study, we applied a high-throughput sequencing approach to examine the presence of picornaviruses in penguins from King George Island, Antarctica. We discovered and characterized a novel picornavirus from cloacal swab samples of gentoo penguins (Pygoscelis papua), which we tentatively named Pingu virus. Also, using RT-PCR we detected this virus in 12.9 per cent of cloacal swabs derived from P. papua, but not in samples from adélie penguins (Pygoscelis adeliae) or chinstrap penguins (Pygoscelis antarcticus). Attempts to isolate the virus in a chicken cell line and in embryonated chicken eggs were unsuccessful. Our results expand the viral diversity, host range, and geographical distribution of the Picornaviridae.

10.
Infect Genet Evol ; 75: 104036, 2019 11.
Artículo en Inglés | MEDLINE | ID: mdl-31499226

RESUMEN

The Dicistroviridae family comprises positive single-stranded RNA viruses that are classified into Picornavirales order. These viruses are identified in arthropod hosts, including some having devastating economic consequences. Here, we described and characterized a novel nearly complete dicistrovirus genome identified in liver samples of velvety free-tailed bats (Molossus molossus) collected in June 2010 in Araçatuba city, São Paulo State, Brazil. This novel virus presents a genome of 9262 nucleotides in length and a typical dicistrovirus genome organization. Based on our phylogenetic analysis and ICTV criteria, we propose this virus as a novel species into the Triatovirus genus. Attempts of viral propagation in Vero E6 and C6/36 cell lines were unsuccessful. The novel dicistrovirus was detected only in one out of nine liver bat samples, representing for the first time an internal organ detection from a representative of this virus family.


Asunto(s)
Quirópteros/virología , Dicistroviridae/clasificación , Dicistroviridae/genética , Animales , Brasil , Biología Computacional/métodos , Genoma Viral , Filogenia , Análisis de Secuencia de ADN , Secuenciación Completa del Genoma
11.
Sci Rep ; 9(1): 5556, 2019 04 03.
Artículo en Inglés | MEDLINE | ID: mdl-30944402

RESUMEN

Birds are the natural reservoir of viruses with zoonotic potential, as well as contributing to the evolution, emergence, and dissemination of novel viruses. In this study, we applied a high-throughput screening approach to identify the diversity of viruses in 118 samples of birds captured between October 2006 to October 2010 in the North and Northeast regions of Brazil. We found nearly complete genomes of novel species of astrovirus and calicivirus in cloacal swabs of ruddy turnstones (Arenaria interpres) collected in Coroa do Avião islet, Pernambuco State. These viruses are positive-sense single-stranded RNA with a genome of ~7 to 8 kb, and were designated as Ruddy turnstone astrovirus (RtAstV) and Ruddy turnstone calicivirus (RTCV), respectively. Phylogenetic analysis showed that RtAstV and RTCV grouped in a monophyletic clade with viruses identified from poultry samples (i.e., chicken, goose, and turkey), including viruses associated with acute nephritis in chickens. Attempts of viral propagation in monkey and chicken cell lines for both viruses were unsuccessful. Also, we found genomes related with viral families that infect invertebrates and plants, suggesting that they might be ingested in the birds' diet. In sum, these findings shed new light on the diversity of viruses in migratory birds with the notable characterization of a novel astrovirus and calicivirus.


Asunto(s)
Astroviridae/genética , Enfermedades de las Aves/virología , Caliciviridae/genética , Charadriiformes/virología , Migración Animal , Animales , Astroviridae/aislamiento & purificación , Infecciones por Astroviridae/veterinaria , Brasil , Caliciviridae/aislamiento & purificación , Infecciones por Caliciviridae/veterinaria , Pollos/virología , Chlorocebus aethiops , Cloaca/virología , Genoma Viral , Secuenciación de Nucleótidos de Alto Rendimiento , Metagenoma , Filogenia , Células Vero
12.
Braz J Microbiol ; 50(2): 547-556, 2019 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-30877663

RESUMEN

Coronaviruses are single-stranded positive-sense RNA viruses associated with important avian diseases. Their relatively high rates of mutation and recombination frequencies allow them to adapt to new hosts and ecological niches. Although Brazil has 18% of global avian species diversity, studies regarding the presence of avian viral diseases in wild birds in South America are scarce. In this study, we performed a retrospective analysis of the presence of CoVs in 746 wild birds. Oropharyngeal and cloacal swabs were obtained and placed together in vials containing VTM transport medium collected in different regions of Brazil between 2006 and 2013. Screening for viral nucleic acid was performed using conventional RT-PCR and pancoronavirus nested PCR. Positive samples were characterized by partial sequencing of the RNA-dependent RNA polymerase (RdRp) gene, and ensuing phylogenetic analysis was performed to investigate the association between virus epidemiology and bird migration routes. Coronavirus RNA were detected and sequenced from six samples, in which three were related to gammacoronaviruses group and the other three to deltacoronavirus group. Our study documents the presence of CoVs related to avian gamma- and deltacoronaviruses circulating in both urban- and poultry-farm regions of Brazil, implicating wild birds as potential carriers of CoVs which may represent a risk to poultry farms and public health in Brazil.


Asunto(s)
Enfermedades de las Aves/epidemiología , Aves/virología , Infecciones por Coronavirus/epidemiología , Coronavirus/aislamiento & purificación , Reservorios de Enfermedades/virología , Gammacoronavirus/aislamiento & purificación , Animales , Animales Salvajes/virología , Secuencia de Bases , Enfermedades de las Aves/virología , Brasil/epidemiología , Coronavirus/genética , Gammacoronavirus/genética , Filogenia , ARN Viral/genética , ARN Polimerasa Dependiente del ARN/genética , Estudios Retrospectivos
13.
J Wildl Dis ; 54(2): 422-425, 2018 04.
Artículo en Inglés | MEDLINE | ID: mdl-29148889

RESUMEN

We tested 529 wild birds captured in northeastern Brazil for infection by avian influenza, Newcastle disease, and West Nile. Viruses were not detected by real-time PCR with the exception of one Tropical Gnatcatcher ( Polioptila plumbea) positive for influenza virus, but this could not be confirmed by viral isolation or gene sequencing.


Asunto(s)
Gripe Aviar/virología , Enfermedad de Newcastle/virología , Virus de la Enfermedad de Newcastle/aislamiento & purificación , Fiebre del Nilo Occidental/virología , Virus del Nilo Occidental/aislamiento & purificación , Animales , Animales Salvajes , Aves , Brasil/epidemiología , Virus de la Influenza A/aislamiento & purificación , Gripe Aviar/epidemiología , Enfermedad de Newcastle/epidemiología , Fiebre del Nilo Occidental/epidemiología
14.
Virology ; 514: 9-17, 2018 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-29128758

RESUMEN

The Anelloviridae comprises single-stranded DNA viruses currently grouped in sixty-eight species classified in twelve genera. They have been found in many vertebrate hosts including primates. In this study, we describe the application of the high-throughput sequencing to examine the frequency and diversity of anelloviruses in rodents, bats and opossums captured in São Paulo State, Brazil. We report a total of twenty-six anelloviruses with sixteen nearly complete genomes and ten partial genomes, which include eleven potential novel species identified in rodents (Cricetidae), bats (Molossidae and Phyllostomidae), and opossums (Didelphidae). We also propose the inclusion of two potential new genera within the Anelloviridae family, provisionally named Omegatorquevirus and Sigmatorquevirus, including six and three novel species of anelloviruses, respectively. In summary, this study expands the diversity and the host range of the known anelloviruses.


Asunto(s)
Anelloviridae/fisiología , Infecciones por Virus ADN/veterinaria , Especificidad del Huésped , Mamíferos/virología , Anelloviridae/clasificación , Anelloviridae/genética , Anelloviridae/aislamiento & purificación , Animales , Biodiversidad , Quirópteros/virología , Infecciones por Virus ADN/virología , Genoma Viral , Mamíferos/clasificación , Zarigüeyas/virología , Filogenia , Roedores/virología
15.
Braz J Microbiol ; 48(4): 747-753, 2017.
Artículo en Inglés | MEDLINE | ID: mdl-28629971

RESUMEN

The red-tailed Amazon parrot (Amazona brasiliensis) is a threatened species of psittacine bird that inhabit coastal regions of Brazil. In view of the threat of this species, the aim of this study was to perform a health evaluation in wild nestlings in Rasa Island, determining the prevalence of enterobacteria and infectious agents according to type of nest. Blood samples were collected from 64 birds and evaluated for antibodies of Chlamydia psittaci by commercial dot-blot ELISA. Cloacal and oropharyngeal swabs samples were collected from 23 birds from artificial wooden nests, 15 birds from PVC nests and 2 birds from natural nests for microbiological analysis. Swab samples were collected from 58 parrots for C. psittaci detection by PCR and from 50 nestlings for Avian Influenza, Newcastle Disease and West Nile viruses' detection analysis by real-time RT-PCR. Ten bacterial genera and 17 species were identified, and the most prevalent were Escherichia coli and Klebsiella oxytoca. There was no influence of the type of nest in the nestlings' microbiota. All samples tested by ELISA and PCR were negative. There is currently insufficient information available about the health of A. brasiliensis and data of this study provide a reference point for future evaluations and aid in conservation plans.


Asunto(s)
Amazona/microbiología , Amazona/virología , Bacterias/aislamiento & purificación , Infecciones Bacterianas/veterinaria , Enfermedades de las Aves/microbiología , Enfermedades de las Aves/virología , Virosis/veterinaria , Virus/aislamiento & purificación , Animales , Animales Salvajes/microbiología , Animales Salvajes/virología , Bacterias/clasificación , Bacterias/genética , Infecciones Bacterianas/microbiología , Brasil , Especies en Peligro de Extinción , Islas , Virosis/virología , Virus/clasificación , Virus/genética
16.
PLoS One ; 12(5): e0177214, 2017.
Artículo en Inglés | MEDLINE | ID: mdl-28486490

RESUMEN

A novel avian paramyxovirus (APMV) isolated from a migratory bird cloacal swab obtained during active surveillance in April 2012 in the Lagoa do Peixe National Park, Rio Grande do Sul state, South of Brazil was biologically and genetically characterized. The nucleotide sequence of the full viral genome was completed using a next-generation sequencing approach. The genome was 14,952 nucleotides (nt) long, with six genes (3'-NP-P-M-F-HN-L-5') encoding 7 different proteins, typical of APMV. The fusion (F) protein gene of isolate RS-1177 contained 1,707 nucleotides in a single open reading frame encoding a protein of 569 amino acids. The F protein cleavage site contained two basic amino acids (VPKER↓L), typical of avirulent strains. Phylogenetic analysis of the whole genome indicated that the virus is related to APMV-10, -2 and -8, with 60.1% nucleotide sequence identity to the closest APMV-10 virus, 58.7% and 58.5% identity to the closest APMV-8 and APMV-2 genome, respectively, and less than 52% identity to representatives of the other APMVs groups. Such distances are comparable to the distances observed among other previously identified APMVs serotypes. These results suggest that unclassified/calidris_fuscicollis/Brazil/RS-1177/2012 is the prototype strain of a new APMV serotype, APMV-15.


Asunto(s)
Migración Animal , Avulavirus/aislamiento & purificación , Aves/virología , Animales , Avulavirus/clasificación , Avulavirus/genética , Aves/fisiología , Genes Virales , Filogenia , América del Sur
17.
J Gen Virol ; 98(2): 225-229, 2017 02.
Artículo en Inglés | MEDLINE | ID: mdl-28284244

RESUMEN

Chapparvoviruses are a highly divergent group of parvoviruses (family Parvoviridae) that have recently been identified via metagenomic sampling of animal faeces. Here, we report the sequences of six novel chapparvoviruses identified through both metagenomic sampling of bat tissues and in silico screening of published vertebrate genome assemblies. The novel chapparvoviruses share several distinctive genomic features and group together as a robustly supported monophyletic clade in phylogenetic trees. Our data indicate that chapparvoviruses have a broad host range in vertebrates and a global distribution.


Asunto(s)
Parvovirinae/clasificación , Parvovirinae/genética , Vertebrados/genética , Vertebrados/virología , Animales , Canarios/genética , Canarios/virología , Cebus/genética , Cebus/virología , Quirópteros/genética , Quirópteros/virología , Simulación por Computador , Evolución Molecular , Orden Génico , Genoma Viral , Metagenómica , Filogenia , Filogeografía
18.
Vector Borne Zoonotic Dis ; 17(4): 271-274, 2017 04.
Artículo en Inglés | MEDLINE | ID: mdl-28103156

RESUMEN

Bats are reservoir hosts for many paramyxoviruses, some of which cause human and zoonotic diseases of public health importance. We developed a Nipah virus nucleoprotein enzyme-linked immunosorbent assay to detect cross-reactive antibodies in serum samples from several bat species in Brazil. Our results warrant further investigation of henipa-like virus reservoirs in the Western hemisphere.


Asunto(s)
Quirópteros/virología , Infecciones por Henipavirus/veterinaria , Henipavirus/inmunología , Animales , Brasil/epidemiología , Infecciones por Henipavirus/epidemiología , Infecciones por Henipavirus/virología
19.
Ecohealth ; 13(4): 813-818, 2016 12.
Artículo en Inglés | MEDLINE | ID: mdl-27645753

RESUMEN

The occurrence of avian influenza viruses (AIV) has been extensively studied in aquatic birds in the Northern hemisphere; however, much less information is available for the South American region. In 2009-2010, we sampled 1006 wild aquatic birds (90% Charadriiformes, 9% Anseriformes, and 1% other groups) at three locations on the Brazilian Amazon coast, a region that serves as a major stop-over and wintering site along the Atlantic Americas flyway. Real-time RT-PCR identified five samples as positive; however, no AIV isolates could be obtained and Illumina sequencing did not produce gene sequences that would allow further characterization of the virus.


Asunto(s)
Anseriformes/virología , Virus de la Influenza A/aislamiento & purificación , Gripe Aviar/epidemiología , Animales , Animales Salvajes , Aves , Brasil/epidemiología
20.
Arch Virol ; 161(11): 3171-7, 2016 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-27558120

RESUMEN

Mosquito-borne alphaviruses are widely distributed throughout the world, causing important human illnesses. Therefore, the development of methods to enable early diagnosis of infections by alphavirus is essential. We show here the development and evaluation of a quantitative real-time RT-PCR using genus-specific primers to the nsP1 viral gene of all mosquito-borne alphaviruses. The specificity and sensitivity of the assay were tested using seven alphaviruses and RNA transcribed from Venezuelan equine encephalitis virus. The detection limits of real-time RT-PCR ranged from 10 to 76 copies per ml. The melting temperature (TM) values for amplification of the alphavirus genomes were 83.05 °C and 85.28 °C. Interestingly, the assay suggested the possibility the arthritogenic alphaviruses with TM peaks of 84.83 to 85.28 °C and encephalitic alphaviruses of 83.34 °C to 84.68 °C could be discriminated both diseases. Real-time RT-PCR may prove very useful for the screening and preliminary diagnosis in outbreaks and surveillance studies as well as for measuring the viral load in pathogenesis studies.


Asunto(s)
Alphavirus/aislamiento & purificación , Culicidae/virología , Reacción en Cadena en Tiempo Real de la Polimerasa/métodos , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Carga Viral/métodos , Alphavirus/genética , Animales , ARN Viral/genética , Sensibilidad y Especificidad , Temperatura de Transición
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