The application of the FLOTAC technique for detection of helminth eggs of medical and veterinary importance in soil samples.

The FLOTAC technique was initially developed to detected infective stages of parasites in animal and human feces. Recently, its applicability has been extended by the use in pastures and vegetables for human consumption. However, its use for the detection of parasites in the soil has never been investigated. In this study, we assessed the performance of the FLOTAC and compared with centrifugal flotation (CFT) and spontaneous sedimentation (SST) techniques. A total of 50 soil samples were collected from the Metropolitan region of Recife, Northeastern Brazil. Initially, samples were standardized, and then assessed in duplicate by the FLOTAC, CFT and SST. Cohen's kappa coefficient, sensitivity, specificity, positive and negative predictive values, and accuracy of the FLOTAC, CFT and SST were assessed using the results of the combination of all three techniques as gold standard. Out of all analyses performed, in 96% (48/50), helminth eggs of parasites were detected. In particular, 96% (48/50) of samples analysed through the FLOTAC technique, 76% (38/50) for CFT and 28% (14/50) for SST were positive. Nematode eggs of the Ancylostomatidae and Ascarididae families were those mostly detected in this study. Overall, the FLOTAC presented high values of sensitivity, demonstrating that this method may also be employed for detection of parasites in soil samples. This study opens new possibilities for the use of the FLOTAC technique, highlighting its role as a potential tool for detecting environmental contamination by parasites of medical and veterinary importance.

An Additional Asset for the FLOTAC Technique: Detection of Gastrointestinal Parasites in Vegetables.

INTRODUCTION: Gastrointestinal parasites are considered amongst the most important threats to veterinary and human health worldwide. The transmission of these parasitic diseases usually involves the accidental ingestion of oocysts, cysts, eggs, and larvae, whose detection is generally achieved by classical coproparasitological methods, based on sedimentation and/or flotation techniques. These procedures might be inefficient or display a low sensitivity when performed on food matrices. This study aimed to assess the value of the FLOTAC technique for the detection of different parasitic stages of gastrointestinal parasites of domestic animals in fresh lettuce. MATERIAL AND METHODS: Samples (n = 100) were purchased from public markets located in the metropolitan region of Recife (Brazil). RESULTS: A total of 79% scored positive for eggs and/or cysts of gastrointestinal parasites. Protozoa and nematodes were detected in 32% (32/100) and 64% (64/100) of samples, respectively, with cysts of the genus Entamoeba (32%) and eggs of nematodes of the order Strongylida (30%) being the most frequently diagnosed. CONCLUSIONS: The findings herein reported demonstrate that the FLOTAC technique can be successfully applied for recovering food-borne parasites of medical and veterinary concern in food matrices.