Influence of polyethylene-type microplastics on long-term exposure to heavy metals in freshwater phytoplankton.

The use of plastic materials has brought about significant social benefits but has also led to negative consequences, particularly their accumulation in aquatic environments. Studies have shown that small plastic particles, known as microplastics (MPs), can carry various harmful pollutants, such as heavy metals (HMs). Therefore, the aim of this research is to investigate the impact of polyethylene-type MPs on the long-term exposure of different HMs on freshwater microalgae Scenedesmus armatus and cyanobacteria Microcystis aeruginosa, in both isolated cultures and phytoplanktonic community conditions. Over a period of 28 days, the strains were subjected to concentrations of Ag+, Cu+2, and Cr+6 corresponding to their respective 72 h-EC10, with or without the presence of MPs. Throughout this period, the growth cell ratio, photosynthetic activity, and reactive oxygen species (ROS) were monitored. The findings indicated a substantial inhibitory impact on cell growth during the initial 7-14 days of exposure, followed by a reduction until reaching values like the controls after 28 days of exposure. There was a disturbance in photosynthetic activity during the first 72 h of exposure, which gradually returned to control levels, mainly significantly affected the respiration phase. Reactive oxygen species (ROS) activity was also affected during the initial 14 days of exposure. The presence or absence of MPs in the culture medium did not significantly alter the observed effects. However, interspecies competition created a more favorable environment for M. aeruginosa over the freshwater microalgae S. armatus. These findings suggest that the formation of MP-HMs complexes may have a limited impact on reducing the adverse effects of HMs in long-term exposures. However, because the impact depends on the specific HM involved, further studies are needed to gain a better understanding of the interaction between these pollutants.

Effects of high-strength landfill leachate effluent on stress-induced microalgae lipid production and post-treatment micropollutant degradation.

This research investigates the effects of landfill leachate effluent concentrations from moving bed biofilm reactor (MBBR) on stress-induced Chlorella vulgaris and Scenedesmus armatus lipid production and post-treatment micropollutant degradation. The effluent concentrations were varied between 25%, 50%, 75%, and 100% (v/v). The landfill leachate influent was treated using two-stage moving bed biofilm reactor under 24 h and 18 h hydraulic retention time (HRT). The results indicated that the effluent concentration was positively correlated with the stress-induced microalgae lipid production in the post-treatment of residual micropollutants. C. vulgaris and S. armatus completely remove residual micropollutants in the effluent. The superoxide dismutase and peroxidase activity were positively correlated with the cellular lipid content. The lipid content of C. vulgaris and S. armatus cultivated in the 18 h HRT effluent were 31-51% and 51-64%, while those in the 24 h HRT effluent were 15-16% and 5-19%. The optimal condition of microalgae cultivation for the post-treatment of residual micropollutants was 50-75% (v/v) effluent concentrations under 18 h HRT, achieving the highest lipid production of 113-116 mg/L for C. vulgaris and 74-75 mg/L for S. armatus. Essentially, the MBBR landfill leachate effluent holds promising potential as a substrate for microalgae lipid production.

Toxic effects of single animal hormones and their mixtures on the growth of Chlorella vulgaris and Scenedesmus armatus.

In their environments, aquatic organisms are simultaneously exposed to mixtures of several endocrine disrupting compounds, including hormones. However, most of the toxicity studies so far focused on effects of single contaminants. The available information on the potential toxicity of combined hormones on microalgae is extremely limited. For these reasons the aim of this study was to evaluate the individual and mixture effect of estrone (E1), ß-estradiol (E2), estriol (E3), 17-α-ethinylestradiol (EE2), progesterone (PRO), 5-pregnen-3ß-ol-20-one (PRE), levonorgestrel (LG) and testosterone (TST) on Chlorella vulgaris and Scenedesmus armatus. Green algae cells were exposed to different concentrations (0.1-100 mg L-1) of hormones for 14 days. Biomass in the form of dry weight and chlorophyll a was examined. The decreasing order of toxicity (based on EC50, 14d) to Chlorella vulgaris and Scenedesmus armatus was: EE2>PRO > E2>PRE > TST > E3>LG > E1 and EE2>PRO > TST > E2>PRE > LG > E1>E3, respectively. Chlorella vulgaris was more sensitive to the effects of hormones than Scenedesmus armatus. Although mixed hormones were more toxic to green algae than single hormones, in the ecosystem mixtures can pose higher ecological risk than single pollutants. Therefore, data on the toxicology of both single and mixed hormones is very valuable for assessment of the possibility of adverse ecological effects caused by these pollutants. Furthermore, these results suggest that environmental exposure to hormone mixtures may cause toxicity levels different to the sum of those of the single hormones and provides a basic understanding of their toxic effect on algae.