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1.
J Hazard Mater ; 480: 135864, 2024 Sep 17.
Artículo en Inglés | MEDLINE | ID: mdl-39298968

RESUMEN

The distribution characteristics of Cr(VI) species in contaminated soil is crucial for soil remediation; however, there is currently a lack of methods for analysing anionic Cr(VI) species in soil. This study has developed a novel sequential extraction method for speciation of Cr(VI) and Cr(III). Besides extraction experiments, simulated chromium species were prepared to verify the presence of proposed chromium species. The results show that Cr(VI) species in soil can be categorized into water-soluble Cr(VI), electrostatically adsorbed Cr(VI), Cr(VI) specifically adsorbed by minerals containing exchangeable Ca2+, Cr(VI) specifically adsorbed by hydrous metal oxides, calcium chromate Cr(VI) and stable complexed adsorption Cr(VI). These Cr(VI) species can be selectively extracted by specific solutions through ion exchange or weak acid dissolution. The most stable Cr(VI) species is Cr(VI) complexed by hydrous iron oxides through bidentate ligand binding; only by dissolution of hydrous iron oxides can this Cr(VI) species be leached. The distribution of Cr(VI) species is closely linked to particular soil compositions including exchangeable Ca2+ and hydrous iron oxides which determinate the Cr(VI) adsorption in soil. Cr(III) species comprise Fe-Cr coprecipitate hydroxides Cr(III), Fe-Mn oxide-bound Cr(III), organic matter-bound Cr(III) and residual Cr(III). Their distribution depends on the types of reductants present in the soil.

2.
Water Res ; 266: 122370, 2024 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-39236505

RESUMEN

Liquid-liquid extraction (LLE) combined with the N2 blow-down method is a promising tool for bioanalysis of drinking water. However, detailed information on which disinfection byproduct (DBP) classes are retained in LLE extracts is currently unavailable. In this study, the recovery of seven classes of volatile DBPs and total adsorbable organic halogens (TOX) during the LLE method, combined with three common N2 blow-down methods, for bioanalysis in real tap water was analyzed at a 2-L scale, along with their corresponding cytotoxicity. The total concentration of seven classes of volatile DBPs in drinking water in Suzhou ranged from 64.6 to 83.0 µg/L, with the majority contributed by trihalomethanes (THMs: 59.9 µg/L), haloaldehydes (HALs: 5.4 µg/L), haloacetamides (HAMs: 3.4 µg/L), and haloacetonitriles (HANs: 3.2 µg/L). During the LLE - N2 blow-down process for bioanalysis, about 69-85 % of targeted volatile DBPs and 64-75 % of TOX were lost, respectively. Seven classes of volatile DBPs accounted for 52.8-64.3 % and 23.8-61.3 % of TOX in tap water and LLE - N2 blow-down samples, respectively, suggesting that targeted aliphatic DBPs are the key contributors to TOX. Furthermore, although LLE - solvent exchange had a better recovery performance than other N2 blow-down methods, the recoveries of volatile DBPs using this method were still not ideal. For example, HALs and HAMs had a slightly better recovery (>50 %), while most volatile DBPs had a poor recovery, including iodo-trihalomethanes (I-THMs, 0 %), haloketones (28 %), THMs (26 %), halonitromethanes (33 %), and HANs (38 %). During LLE - solvent exchange, 31 % and 36 % of targeted DBPs and TOX, respectively, in real tap water can be retained, which shows better performance than non-ionic macroporous copolymers (XAD). More importantly, the water volume required in this method for cytotoxicity analysis is 2 L, which greatly reduces the burden of water sample collection, transport, and pre-treatment compared to XAD (which typically requires 5 or 10 L). In general, this paper reveals the fate of volatile DBPs during LLE - N2 blow-down and indicates that LLE - solvent exchange is a good substitute for the XAD method in bioanalysis.

3.
J Chromatogr A ; 1735: 465297, 2024 Oct 25.
Artículo en Inglés | MEDLINE | ID: mdl-39243588

RESUMEN

As a naturally widely-occurring dietary, cosmetic, and therapeutic flavonoid, kaempferol has gained much consideration for its nutritional and pharmaceutical properties in recent years. Although there have been performed a high number of studies associated with different aspects of kaempferol's analytical investigations, the lack of a comprehensive summary of the various methods and other plant sources that have been reported for this compound is being felt, especially for many biological applications. This study, aimed to provide a detailed compilation consisting of sources (plant species) and analytical information that was precisely related to the natural flavonoid (kaempferol). There is a trend in analytical research that supports the application of modern eco-friendly instruments and methods. In conclusion, ultrasound-assisted extraction (UAE) is the most general advanced method used widely today for the extraction of kaempferol. During recent years, there is an increasing tendency towards the identification of kaempferol by different methods.


Asunto(s)
Quempferoles , Quempferoles/aislamiento & purificación , Quempferoles/análisis , Extractos Vegetales/química , Extractos Vegetales/aislamiento & purificación , Extractos Vegetales/análisis , Fraccionamiento Químico/métodos , Cromatografía Líquida de Alta Presión/métodos
4.
Sensors (Basel) ; 24(15)2024 Jul 31.
Artículo en Inglés | MEDLINE | ID: mdl-39124009

RESUMEN

The intrusion of objects into track areas is a significant issue affecting the safety of urban rail transit systems. In recent years, obstacle detection technology based on LiDAR has been developed to identify potential issues, in which accurately extracting the track area is critical for segmentation and collision avoidance. However, because of the sparsity limitations inherent in LiDAR data, existing methods can only segment track regions over short distances, which are often insufficient given the speed and braking distance of urban rail trains. As such, a new approach is developed in this study to indirectly extract track areas by detecting references parallel to the rails (e.g., tunnel walls, protective walls, and sound barriers). Reference point selection and curve fitting are then applied to generate a reference curve on either side of the track. A centerline is then extrapolated from the two curves and expanded to produce a 2D track area with the given size specifications. Finally, the 3D track area is acquired by detecting the ground and removing points that are either too high or too low. The proposed technique was evaluated using a variety of scenes, including tunnels, elevated sections, and level urban rail transit lines. The results showed this method could successfully extract track regions from LiDAR data over significantly longer distances than conventional algorithms.

5.
Int J Biol Macromol ; 278(Pt 1): 134463, 2024 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-39102920

RESUMEN

Previous studies have shown that modified citrus pectin (MCP) is an anti-tumor material of food grade. In this study, two enzymatically modified Ougan (Citrus Suavissima Hort. ex Tanaka) peel pectins (EMP1 and EMP2, the ones extracted by alkali and enzymatic methods) were used to investigate their differential effects on viability and physiology of Hela cells. The results showed that EMP1 and EMP2 had 88.00 % and 81.01 % galacturonic acid, 21.31 % and 20.25 % esterification degree, 10,417 g/mol and 6416 g/mol molecular weight (Mw), 82.86 % and 50.62 % RG-I, and 8.91 % and 15.70 % HG, respectively. EMP2 had higher intensities of absorption peaks than EMP1. They were irregularly shaped, with more holes on EMP1 but more wrinkles on EMP2. Both could inhibit the growth, proliferation, migration, and invasion of HeLa cells in a concentration-dependent manner, with better efficiency in EMP2. Meanwhile, EMP2 was more efficient than EMP1 in blocking the cell cycle in S phase, resulting in apoptosis. In conclusion, the variations caused by extraction resulted in differences in anti-tumor activity of MCP and EMP2 with lower Mw and higher HG exhibited better anti-tumor effects. This study would provide an experimental basis and reference for the research and development of anti-tumor supplements from citrus pectin.


Asunto(s)
Proliferación Celular , Citrus , Pectinas , Humanos , Células HeLa , Pectinas/farmacología , Pectinas/química , Citrus/química , Proliferación Celular/efectos de los fármacos , Movimiento Celular/efectos de los fármacos , Apoptosis/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Ciclo Celular/efectos de los fármacos
6.
Environ Sci Pollut Res Int ; 31(35): 47552-47583, 2024 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-39034377

RESUMEN

In recent years, consumer preferences have begun to turn back to natural dyes, whereas synthetic dyes have been pushed into the background over the previous 60 years. This is a result of increased knowledge of the potential hazards associated with the creation of synthetic dyes, which use raw materials derived from petrochemicals and involve intense chemical interactions. Such dyes need a lot of energy to produce, and their negative effects on the environment increase pollution. It has been discovered that several of these dyes, particularly the azo-based ones are carcinogenic. On the contrary, natural dyes are getting more attention from scientists and researchers as a result of their several advantages like being eco-friendly, biodegradable and renewable, sustainable, available in nature, having no disposal problems, minimizing the consumption of fossil fuel, anti-bacterial, insect repellent, and anti-allergic, anti-ultraviolet, intensify dyeing and finishing process efficiency, less expensive, and no adverse effects on human health and environment. However, there are also some drawbacks, like poor fastness properties, natural dye printing for bulk production, difficulties in reproducibility of shades, and so forth. Despite all these limitations, the demand for natural dyes is increasing significantly in textile industries because they offer far more safety than synthetic dyes. This study provides an overall concept of the natural dyes in textile printing. It illustrates parameters of printing performance, methods, and techniques of extraction of natural dyes, printing methods, and printing of natural and synthetic fibers. Finally, this study describes the challenges and future prospects of natural dyes in textile printing.


Asunto(s)
Colorantes , Textiles , Colorantes/química , Impresión , Industria Textil
7.
Materials (Basel) ; 17(13)2024 Jul 03.
Artículo en Inglés | MEDLINE | ID: mdl-38998357

RESUMEN

Aiming at the problems of the large storage, complex composition, low comprehensive utilization rate, and high environmental impact of coal gangue, this paper carried out experimental research on the preparation of iron oxide red from high-iron gangue by calcination activation, acid leaching, extraction, and the hydrothermal synthesis of coal gangue. The experimental results show that when the calcination temperature of coal gangue is 500 °C, the calcination time is 1.5 h, the optimal concentration of iron removal is 6 mol/L, the acid leaching temperature is 80 °C, the acid leaching time is 1 h, and the liquid--solid mass ratio is 4:1; the iron dissolution rate can reach 87.64%. A solvent extraction method (TBP-SK-hydrochloric acid system) was used to extract the leachate, and a solution with iron content up to 99.21% was obtained. By controlling the optimum hydrothermal conditions (pH = 9, temperature 170 °C, reaction time 5 h), high-purity iron oxide red product can be prepared; the yield is 80.07%. The red iron oxide was characterized by XRD, SEM-EDS, particle-size analysis, and ICP-OES. The results show that the red iron oxide peak has a cubic microstructure, an average particle size of 167.16 µm, and a purity of 99.16%. The quality of the prepared iron oxide red product meets the requirement of 98.5% of the "YHT4 Iron oxide Standard for ferrite". It can be used as a raw material to produce high-performance soft magnetic ferrite. In summary, this experimental study on the preparation of iron oxide red from coal gangue is of great significance for the comprehensive utilization of coal gangue to realize the sustainable development of the environment and economy.

8.
Planta ; 260(2): 40, 2024 Jul 02.
Artículo en Inglés | MEDLINE | ID: mdl-38954049

RESUMEN

MAIN CONCLUSION: Rainwater most probably constitutes a relatively effective solvent for lichen substances in nature which have the potential to provide for human and environmental needs in the future. The aims were (i) to test the hypothesis on the potential solubility of lichen phenolic compounds using rainwater under conditions that partly reflect the natural environment and (ii) to propose new and effective methods for the water extraction of lichen substances. The results of spectrophotometric analyses of total phenolic metabolites in rainwater-based extracts from epigeic and epiphytic lichens, employing the Folin-Ciocalteu (F.-C.) method, are presented. The water solvent was tested at three pH levels: natural, 3, and 9. Extraction methods were undertaken from two perspectives: the partial imitation of natural environmental conditions and the potential use of extraction for economic purposes. From an ecological perspective, room-temperature water extraction ('cold' method) was used for 10-, 60-, and 120-min extraction periods. A variant of water extraction at analogous time intervals was an 'insolation' with a 100W light bulb to simulate the heat energy of the sun. For economic purposes, the water extraction method used the Soxhlet apparatus and its modified version, the 'tea-extraction' method ('hot' ones). The results showed that those extractions without an external heat source were almost ineffective, but insolation over 60- and 120-min periods proved to be more effective. Both tested 'hot' methods also proved to be effective, especially the 'tea-extraction' one. Generally, an increase in the concentration of phenolic compounds in water extracts resulted from an increasing solvent pH. The results show the probable involvement of lichen substances in biogeochemical processes in nature and their promising use for a variety of human necessities.


Asunto(s)
Líquenes , Fenoles , Solubilidad , Espectrofotometría , Agua , Líquenes/química , Líquenes/metabolismo , Fenoles/metabolismo , Fenoles/análisis , Agua/química , Solventes/química , Concentración de Iones de Hidrógeno , Lluvia/química
9.
Carbohydr Polym ; 341: 122351, 2024 Oct 01.
Artículo en Inglés | MEDLINE | ID: mdl-38876719

RESUMEN

The importance of renewable resources and environmentally friendly materials has grown globally in recent time. Hemicellulose is renewable lignocellulosic materials that have been the subject of substantial valorisation research. Due to its distinctive benefits, including its wide availability, low cost, renewability, biodegradability, simplicity of chemical modification, etc., it has attracted increasing interest in a number of value-added fields. In this review, a systematic summarizes of the structure, extraction method, and characterization technique for hemicellulose-based materials was carried out. Also, their most current developments in a variety of value-added adsorbents, biomedical, energy-related, 3D-printed materials, sensors, food packaging applications were discussed. Additionally, the most recent challenges and prospects of hemicellulose-based materials are emphasized and examined in-depth. It is anticipated that in the near future, persistent scientific efforts will enable the renewable hemicellulose-based products to achieve practical applications.

10.
MethodsX ; 12: 102758, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38883586

RESUMEN

Next-generation sequencing requires intact and high-quality DNA. However, typical liquid-nitrogen DNA extraction methods are expensive and not practical for field sample collections. Hence, we present a cost-effective method for DNA extraction from silica-dried leaf samples, eliminating the need for liquid nitrogen. Two protocols were evaluated to determine the effectiveness of grinding dried plant samples without liquid nitrogen in comparison to the standard protocol for tissue homogenization and cell lysis. Protocol 1 involved grinding fresh leaf samples with liquid nitrogen, while Protocol 2 entailed incubating dried plant samples at-20 °C for 1 h before grinding in the absence of liquid nitrogen. Both protocols produced comparable DNA yields with an average A260/A280 ratio of 1.78±0.02, suitable for short- and long-read sequencing. Using Protocol 2, we successfully assembled ten plastomes. It also demonstrated versatility as comparable DNA quality was obtained from dried mollusks and actinomycetes, resulting in the successful assembly of two complete mitochondrial genomes. The protocol is advantageous for research workflows involving the collection of samples in the field as a long-term source of genetic material.•Drying: Fresh samples were silica-dried at silica-to-sample ratio of 2:1.•Pre-lysis: Dried samples were frozen at -20 °C for 1 hour before grinding.•Frozen samples were subjected to tissue homogenization followed by the standard CTAB DNA extraction.

11.
J Environ Manage ; 365: 121247, 2024 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-38909573

RESUMEN

Clarifying the occurrence and morphological characteristics of petroleum hydrocarbons (PHs) in soil can facilitate a comprehensive understanding of their migration and transformation patterns in soil/sediment. Additionally, by establishing the dynamic transformation process of each occurrence state, the ecological impact and environmental risk associated with PHs in soil/sediment can be assessed more precisely. The adsorption experiments and closed static incubation experiments was carried out to explore the PHs degradation and fraction distribution in aged contaminated soil under two remediation scenarios of natural attenuation (NA) and bioaugmentation (BA) by exogenous bacteria through a new sequential extraction method based on Tenax-TA, Hydroxypropyl-ß-cyclodextrin and Rhamnolipid (HPCD/RL), accelerated solvent extractor (ASE) unit and alkaline hydrolysis extraction. The adsorption experiment results illustrated that bioaugmentation could promote the desorption of PHs in the adsorption phase, and the soil-water partition coefficient Kd decreased from 0.153 L/g to 0.092 L/g. The incubation experiment results showed that compared with natural attenuation, bioaugmentation could improve the utilization of PHs in aged soil and promote the generation of non-extractable hydrocarbons. On the 90th day of the experiment, the concentrations of weakly adsorbed hydrocarbons in the natural attenuation and bioaugmentation experimental groups decreased by 46.44% and 87.07%, respectively, while the concentrations of strongly adsorbed hydrocarbons and non-extractable hydrocarbons increased by 77.93%, 182.14%, and 80.91%, and 501.19%, respectively, compared their initial values. We developed a novel dynamic model and inverted the kinetic parameters of the model by the parameter scanning function and the Markov Chain Monte Carlo (MCMC) method based on the Bayesian approach in COMSOL Multiphysics® finite element software combined with experimental data. There was a good linear relationship between experimental interpolation data and model prediction data. The R2 for the concentrations of weakly adsorbed hydrocarbons ranged from 0.9953 to 0.9974, for strongly adsorbed hydrocarbons from 0.9063 to 0.9756, and for non-extractable hydrocarbons from 0.9931 to 0.9982. These extremely high correlation coefficients demonstrate the high accuracy of the parameters calculated using the Bayesian inversion method.


Asunto(s)
Biodegradación Ambiental , Hidrocarburos , Petróleo , Contaminantes del Suelo , Suelo , Contaminantes del Suelo/metabolismo , Petróleo/metabolismo , Hidrocarburos/metabolismo , Suelo/química , Adsorción , Glucolípidos
12.
Ultrason Sonochem ; 108: 106948, 2024 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-38878717

RESUMEN

In this study, the process conditions, physicochemical properties, structural composition and activity of polysaccharides isolated from leechee peel (LPP) by ultrasound-assisted extraction (UAE) with enzyme and alkali solution extraction (ASE) were compared. The results showed that the total sugar content of LPP extracted by UAE accounted for 75.65 %, which was significantly higher than that extracted by alkali solution. The optimum conditions were as follows: extraction temperature of 68.78 ℃, ultrasonic enzymolysis time of 39.68 min, pectinase dosage of 4.03 %, solid-liquid ratio of 1:30 g/mL, and ultrasonic power of 360 W. The antioxidant activities and structure of leechee peel polysaccharide (LPP) prepared under different conditions were compared. It was found that UAE-LPP was an α-type polysaccharide containing 15.83 % uronic acid. Moreover, LPP extracted by UAE showed strong activity in anti-lipid peroxidation and reducing ability. Ultrasound-assisted enzymatic method is an effective means to improve the content and activity of natural plant polysaccharides, and this method has the advantages of short time-consuming, simple process and easy operation, which can greatly improve the utilization rate of polysaccharides and lay a theoretical and scientific basis for the development and utilization of LPP.


Asunto(s)
Antioxidantes , Fraccionamiento Químico , Polisacáridos , Ondas Ultrasónicas , Polisacáridos/química , Polisacáridos/aislamiento & purificación , Fraccionamiento Químico/métodos , Antioxidantes/química , Antioxidantes/aislamiento & purificación , Poligalacturonasa , Temperatura , Peroxidación de Lípido/efectos de los fármacos
13.
Methods Mol Biol ; 2820: 21-28, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38941011

RESUMEN

The metaproteomic approach allows a deep microbiome characterization in different complex systems. Based on metaproteome data, microbial communities' composition, succession, and functional role in different environmental conditions can be established.The main challenge in metaproteomic studies is protein extraction, and although many protocols have been developed, a few are focused on the protein extraction of fermented foods. In this chapter, a reproducible and efficient method for the extraction of proteins from a traditionally fermented starchy food is described. The method can be applied to any fermented food and aims to enrich the extraction of proteins from microorganisms for their subsequent characterization.


Asunto(s)
Alimentos Fermentados , Proteómica , Alimentos Fermentados/microbiología , Alimentos Fermentados/análisis , Proteómica/métodos , Fermentación , Proteínas/aislamiento & purificación , Proteínas/análisis , Microbiota , Microbiología de Alimentos/métodos
14.
Heliyon ; 10(10): e31365, 2024 May 30.
Artículo en Inglés | MEDLINE | ID: mdl-38818193

RESUMEN

Goupi plaster, a representative preparation of black plaster, has demonstrated promising effects in treating knee osteoarthritis. However, high temperature used in traditional frying extraction may cause decomposition of its effective components, thus limiting the efficacy. This study aimed to explore the scientific nature of the traditional preparation technology of Goupi plaster, and to compare the effects of different extraction methods on the types of chemical components and the content of index components. The UPLC-Q-Exactive-MS and UPLC-MS/MS technologies which have high efficiency, sensitivity and accuracy, were used to qualitatively and quantitatively analyze the chemical components of Goupi plaster under different preparation processes. The results show that the extraction solvent approach is different from the traditional frying extraction method, and has a positive effect. However, the mechanism of action of Goupi plaster is complex and its pharmacological effects are diverse. Future studies should explore whether it necessary to change the frying extraction method. This experiment provides a theoretical basis that will guide further scientific discussion and research into the frying extraction of Goupi plaster.

15.
Int J Mol Sci ; 25(10)2024 May 14.
Artículo en Inglés | MEDLINE | ID: mdl-38791411

RESUMEN

Melon (Cucumis melo L.) is a global commercial crop that is sensitive to seed-borne wilt infections caused by Fusarium oxysporum f. sp. melonis (Fom). To address the challenge of detecting Fom contamination, we designed a probe-based real-time PCR method, TDCP2, in combination with rapid or column-based DNA extraction protocols to develop reliable molecular detection methods. Utilizing TDCP2, the detection rate reached 100% for both artificially Fom-inoculated (0.25-25%) and pod-inoculated melon seeds in conjunction with DNA samples from either the rapid or column-based extraction protocol. We performed analyses of precision, recall, and F1 scores, achieving a maximum F1 score of 1 with TDCP2, which highlights the robustness of the method. Additionally, intraday and interday assays were performed, which revealed the high reproducibility and stability of column-based DNA extraction protocols combined with TDCP2. These metrics confirm the reliability of our developed protocols, setting a foundation for future enhancements in seed pathology diagnostics and potentially broadening their applicability across various Fom infection levels. In the future, we hope that these methods will reduce food loss by improving the control and management of melon diseases.


Asunto(s)
Fusarium , Enfermedades de las Plantas , Reacción en Cadena en Tiempo Real de la Polimerasa , Semillas , Fusarium/genética , Fusarium/aislamiento & purificación , Semillas/microbiología , Enfermedades de las Plantas/microbiología , Reacción en Cadena en Tiempo Real de la Polimerasa/métodos , Cucurbitaceae/microbiología , ADN de Hongos/genética , ADN de Hongos/aislamiento & purificación , Cucumis melo/microbiología , Reproducibilidad de los Resultados
16.
Chemosphere ; 359: 142264, 2024 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-38714248

RESUMEN

Extracellular DNA refers to DNA fragments existing outside the cell, originating from various cell release mechanisms, including active secretion, cell lysis, and phage-mediated processes. Extracellular DNA serves as a vital environmental biomarker, playing crucial ecological and environmental roles in water bodies. This review is summarized the mechanisms of extracellular DNA release, including pathways involving cell lysis, extracellular vesicles, and type IV secretion systems. Then, the extraction and detection methods of extracellular DNA from water, soil, and biofilm are described and analyzed. Finally, we emphasize the role of extracellular DNA in microbial community systems, including its significant contributions to biofilm formation, biodiversity through horizontal gene transfer, and electron transfer processes. This review offers a comprehensive insight into the sources, distribution, functions, and impacts of extracellular DNA within aquatic environments, aiming to foster further exploration and understanding of extracellular DNA dynamics in aquatic environments as well as other environments.


Asunto(s)
Aguas Residuales , ADN Ambiental/análisis , Biopelículas , Biodiversidad , Monitoreo del Ambiente/métodos , Transferencia de Gen Horizontal , Eliminación de Residuos Líquidos/métodos
17.
Plant Methods ; 20(1): 70, 2024 May 16.
Artículo en Inglés | MEDLINE | ID: mdl-38755668

RESUMEN

BACKGROUND: Phytophthora sojae, a soil-borne oomycete pathogen, has been a yield limiting factor for more than 60 years on soybean. The resurgence of P. sojae (Phytophthora sojae) is primarily ascribed to the durable oospores found in soil and remnants of the disease. P. sojae is capable of infesting at any growth periods of the soybean, and the succeed infestation of P. sojae is predominantly attributed to long-lived oospores present in soil. Comprehending the molecular mechanisms that drive oospores formation and their significance in infestation is the key for effective management of the disease. However, the existing challenges in isolating and extracting significant quantities of oospores pose limitations in investigating the sexual reproductive stages of P. sojae. RESULTS: The study focused on optimizing and refining the culture conditions and extraction process of P. sojae, resulting in establishment of an efficient and the dependable method for extraction. Novel optimized approach was yielded greater quantities of high-purity P. sojae oospores than traditional methods. The novel approach exceeds the traditional approaches with respect to viability, survival ability, germination rates of new oospores and the pathogenicity of oospores in potting experiments. CONCLUSION: The proposed method for extracting P. sojae oospores efficiently yielded a substantial quantity of highly pure, viable, and pathogenic oospores. The enhancements in oospores extraction techniques will promote the research on the sexual reproductive mechanisms of P. sojae and lead to the creation of innovative and effective approaches for managing oomycete diseases.

18.
Sci Rep ; 14(1): 10899, 2024 05 13.
Artículo en Inglés | MEDLINE | ID: mdl-38740854

RESUMEN

In order to obtain the best mass spectrometry identification results for using the most appropriate methods in clinical practice, we explore the optimal pretreatment methods for different species and morphologies of filamentous fungi. 98 fungal strains were treated with formic acid sandwich method, dispersion method, extraction method, and other methods using a medium element mass spectrometer (EXS3000) as a platform. Each strain had three targets, and the identification rates and confidence differences under different pre-treatment methods were compared to evaluate the identification effects of these methods. The mass spectrometry identification rates of 98 filamentous fungi obtained after pre-treatment with formic acid sandwich method, dispersion method, and extraction method were 85.71%, 82.65%, and 75.51%, respectively. The identification rate of the formic acid sandwich method was significantly higher than the other two methods (P < 0 005) has the best identification ability and the obtained confidence is also higher than the other two methods. The use of formic acid sandwich method for mass spectrometry identification of filamentous fungi can achieve ideal identification results, which is suitable for mass spectrometry identification of filamentous fungi in conventional laboratories.


Asunto(s)
Hongos , Espectrometría de Masas , Hongos/aislamiento & purificación , Hongos/clasificación , Espectrometría de Masas/métodos , Formiatos/química , Formiatos/análisis , Micosis/microbiología , Micosis/diagnóstico , Humanos
19.
Curr Res Food Sci ; 8: 100753, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38725963

RESUMEN

Camellia oleifera oil is a pure and natural high-grade oil prevalent in South China. Camellia oleifera oil is known for its richness in unsaturated fatty acids and high nutritional value. There is increasing evidence indicating that a diet rich in unsaturated fatty acids is beneficial to health. Despite the widespread production of Camellia oleifera oil and its bioactive components, reports on its nutritional components are scarce, especially regarding systematic reviews of extraction methods and biological functions. This review systematically summarized the latest research on the bioactive components and biological functions of Camellia oleifera oil reported over the past decade. In addition to unsaturated fatty acids, Camellia oleifera oil contains six main functional components contributing to its antioxidant, antibacterial, anti-inflammatory, antidiabetic, anticancer, neuroprotective, and cardiovascular protective properties. These functional components are vitamin E, saponins, polyphenols, sterols, squalene, and flavonoids. This paper reviewed the biological activity of Camellia oleifera oil and its extraction methods, laying a foundation for further development of its bioactive components.

20.
Heliyon ; 10(9): e30118, 2024 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-38726106

RESUMEN

As economic power increases and market patterns adapt, labor becomes an increasingly significant factor of production. However, there is a dearth of discourse regarding the structural changes that have occurred in the correlation of the labor force across industries, as well as a visual representation of the labor force's movement across industries. To quantify and analyze the correlation effect with greater precision, it is necessary to establish an input-output model as the foundation of analysis, comparing the changes in the total output of the economic system prior to and subsequent to the exclusion using the vertical integration algorithm. By decomposing the path structure, the average propagation distance of the labor force population's demand for each industry can be determined. By employing labor force population data from the corresponding years and China's input-output tables published by the National Bureau of Statistics of China (NBS) from 2005 to 2020, this study conducts a quantitative analysis of the correlation effect between labor force population and the trend of its transfer across 19 industries. The findings indicate that the correlation effect between labor force and population is most pronounced in the manufacturing sector. Furthermore, the construction sector faces an especially critical requirement for labor force personnel from other industries. The article culminates with a recommendation that the government enhance its macro-control endeavors to address labor market risk shocks and take an active stance in response to labor market fluctuations.

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