Correcting angular distortions in Bragg coherent X-ray diffraction imaging.

Bragg coherent X-ray diffraction imaging (BCDI) has emerged as a powerful technique for strain imaging and morphology reconstruction of nanometre-scale crystals. However, BCDI often suffers from angular distortions that appear during data acquisition, caused by radiation pressure, heating or imperfect scanning stages. This limits the applicability of BCDI, in particular for small crystals and high-flux X-ray beams. Here, we present a pre-processing algorithm that recovers the 3D datasets from the BCDI dataset measured under the impact of large angular distortions. We systematically investigate the performance of this method for different levels of distortion and find that the algorithm recovers the correct angles for distortions up to 16.4× (1640%) the angular step size dθ = 0.004°. We also show that the angles in a continuous scan can be recovered with high accuracy. As expected, the correction provides marked improvements in the subsequent phase retrieval.

Comprehensive insights into phosphorus solubility and organic matter's impact on black phosphate leaching.

The current study introduces groundbreaking insights into how organic matter (OM) of the black phosphate (RB-Ph) uniquely influences phosphorus (P) solubility during acetic acid (AA) leaching, expanding our understanding in this crucial area. To highlight such role, the OM of the RB-Ph was treated separately by different procedures including calcination at 550 â„ƒ/4 h (CB-Ph), 30% hydrogen peroxide (HB-Ph) and intensive grinding to nano-sizes (NB-Ph). The mineralogical, chemical and morphological characteristics of phosphatic and non-phosphatic components of these phosphatic materials were carefully examined pre- and post-treatment via different techniques. The P dissolution of the precursor RB-Ph and its modified derivatives all over the applied experimental parameters traced the following trend: NB-Ph > RB-Ph > CB-Ph > HB-Ph. Intensive grinding to nanoscale resulted in amorphous components with conspicuous OM content (TOC, 0.410%), significantly enhanced P dissolution rate of NB-Ph (730-980 ppm), despite the noticeable reduction in its P2O5 content to 22.34 wt.%. The precursor RB-Ph, thanks to its high OM content (TOC, 0.543%), also displayed a sufficient P dissolution rate (470-750 ppm) compared to the two other modified derivatives, CB-Ph (410-700 ppm) and HB-Ph (130-610 ppm). Such deep and conspicuous impact of OM on P solubility can be tied to their decomposition, releasing not only organic acids but also the adsorbed P by the OM's surficial binding sites to the solution. Finally, the optimum conditions of P leaching were attained at 2:1 acid/solid (w/w) ratio and 2 h of retention time of all investigated samples.

Translation and validation of the Neurological Assessment in Neuro-Oncology scale to Brazilian Portuguese.

Introduction: The Neurological Assessment for Neuro-Oncology (NANO) scale was elaborated to assess neurologic function in integration with radiological criteria to evaluate neuro-oncological patients in clinical setting and enable the standardization of neurological assessment in clinical trials. The objective of this study is the translation to Brazilian Portuguese and transcultural adaptation of NANO scale in patients with the diagnosis of glioblastoma, brain metastasis and low-grade glioma. Methods: Patients with diagnosis of glioblastoma, brain metastasis, and low-grade glioma were prospectively evaluated between July 2019 and July 2021. The process of translating and cross-culturally adapting the NANO scale included: translation from English to Portuguese, synthesis and initial revision by an expert committee, back-translation from Portuguese to English, a second revision by the expert committee, and the application of the NANO scale. Regarding the reliability of the NANO scale, Cronbach's alpha was employed to measure the internal consistency of all scale items and assess the impact of item deletion. Additionally, Spearman's correlation test was used to evaluate the convergent validity between the NANO scale and Karnofsky Performance Scale (KPS). Results: One hundred and seventy-four patients were evaluated. A statistically significant inverse relation (p < 0.001) between KPS and NANO scale was founded. The Cronbach's alpha values founded for NANO scale were 0.803 for glioblastoma, 0.643 for brain metastasis, and 0.482 for low grade glioma. Discussion: The NANO scale Brazilian Portuguese version proves to be reproducible and valid to evaluate neuro-oncological patients with glioblastoma and brain metastasis, presenting a strong correlation with KPS scale. Further studies are warranted to assess the validity and reliability of the scale in patients diagnosed with low-grade glioma.

White analytical insight for sensitive fluorescent determination of semaglutide and tirzepatide in pharmaceuticals and biological matrices.

The present study is focused on the sensitive determination of newly FDA-approved glucagon-like-peptide agonists semaglutide (SEM) and tirzepatide (TIR). Direct, selective and label-free spectrofluorometric method was proposed and validated (according to ICH guidelines) for determination SEM and TIR in their pure form, newly approved pharmaceuticals and spiked human plasma. The developed method was based on measuring the native fluorescence of SEM and TIR in ethanol at 294.8 and 303 nm after being excited at 216 and 225 nm for SEM and TIR in order. The method sensibility allowed the quantification of both drugs in nano-scale up to 10 ng/mL. Several experimental variables including solvent type, surfactant, and pH were optimized after several attempts to get the best sensitivity for both drugs. The mean recovery percentage of SEM was compared and found in agreement with the reported method using student's t-test and the variance ratio F-test. Additionally, the greenness and whiteness profiles for this approach were evaluated using the GAPI, AGREE, and RGB algorithm; the positive results supported its use as great candidates for successful implementation in quality control labs and the pharmaceutical analysis companies.

Insights into mechanism and modelling of dynamic moisture sorption in dual-porous insulation materials with micro- and nano-scale pores.

HYPOTHESIS: Understanding moisture sorption in porous insulation materials is challenging due to the influence of multiscale pore structures on phase behavior and transport properties. Dynamic moisture sorption in dual-porous materials is likely co-determined by interior micro- and nano-scale pores, and an accurate physical model for predicting moisture evolution can be developed by clarifying the sorption mechanisms. EXPERIMENTS: Moisture behavior during the dynamic sorption of dual-porous insulation material is measured by low-field nuclear magnetic resonance (NMR) experiments. The contributions of micro- and nano-scale pores to the adsorbed moisture are differentiated using NMR relaxometry, and the evolution of moisture morphology is quantitatively analyzed. FINDINGS: Analysis of T2 evolution reveals that the moisture in nano-scale pores alters from adsorption layers to liquid with increasing relative humidity (RH), while minimal sorption occurs in micro-scale pores. Moisture is mainly transferred as vapor molecules at low RH levels, with the dynamic sorption enhanced by molecular diffusion in micro-scale pores. Capillary flow in nano-scale pores dominates moisture transport when RH rises above a threshold, leading to a significant increase in apparent moisture diffusivity. According to the elucidated mechanism, a physical model is further developed to predict moisture sorption inside dual-porous insulation materials, and it may serve as a basis for evaluating and optimizing the performance of dual-porous systems in different environments.

Long-term stability and toxicity effects of three-dimensional electrokinetic remediation on chromium-contaminated soils.

The three-dimensional electrokinetic remediation (3D EKR) achieved efficient removal of chromium (Cr) from the soil through mechanisms including electromigration, electroosmosis, and redox reactions. In this study, the long-term stability, leaching toxicity, bioavailability, and phytotoxicity of Cr in remediated soils were systematically analyzed to comprehensively evaluate the effectiveness of the 3D EKR method. The results showed that the concentration of hexavalent chromium (Cr (VI)) in the leachate of the 3D EKR system with sulfidated nano-scale zerovalent iron (S-nZVI) was more than 40% lower than those of the other 3D electrode groups, and the time required to reach the level III standard of groundwater quality criterion in China (0.05 mg/L, GB/T 14848-2017) was significantly shortened. The stabilization of Cr(VI) in contaminated soil after 3D EKR was maintained for 300 pore volumes (PVs), indicating that the treated Cr(VI) had good long-term stability. The leaching toxicity and bioaccessibility of Cr were assessed by the synthetic precipitation leaching procedure (SPLP), the toxicity characteristic leaching procedure (TCLP), and the physiologically based extraction test (PBET). The concentration of Cr(VI) in the SPLP, TCLP, and PBET leachates of the S-nZVI group decreased by more than 25% compared to the other 3D electrode groups, corresponding to the decrease in leaching toxicity and bioavailability of the treated Cr during the 15-day remediation period. In addition, the germination rate of wheat seeds and the average biomass of wheat seedlings in the S-nZVI group under alkaline conditions (EE) were higher than those in the non-polluting group (Blank-OH), indicating that the remediated soil had no obvious toxicity to wheat. In summary, 3D EKR achieved a satisfactory and stable remediation effect on Cr-contaminated soil, especially when using S-nZVI as the 3D electrode.

Regulating Lithium Transfer Pathway to Avoid Capacity Fading of Nano Si Through Sub-Nano Scale Interfused SiOx /C Coating.

Si nanoparticles (NPs) are considered as a promising high-capacity anode material owing to their ability to prevent mechanical failure from drastic volume change during (de)lithiation. However, upon cycling, a quick capacity fading is still observed for Si NPs, and the underlying mechanism remains elusive. In this contribution, it is demonstrated that the quick capacity fading is mainly caused by the generation of dead (electrochemically inert) Si with blocked electron conductivity in a densely composited Si/SEI (solid electrolyte interface) hybrid. This is due to the combined influence of electrolyte-related side reactions and the accompanied agglomeration of Si NPs. A compact, sub-nano scale interfused SiOx /C composite coating onto the Si NPs is constructed, and a highly stabilized electrochemistry is achieved upon long cycling. The SiOx /C coating with electron/ion dual transport paths and robust mechanical flexibility enables a fast and stable lithium ion/electron dual diffusion pathway towards the encapsulated Si. With fast reaction kinetics, stable SEI, and an antiagglomeration feature, the obtained Si@SiOx /C composite demonstrates a stable high capacity. This work unravels new perspectives on the capacity fading of Si NPs and provides an effective encapsulating method to remedy the structure degradation and capacity fading of nano Si.

A Submicron-Scale Plugging Agent for Oil-Based Drilling Fluid Synthesized Using the Inverse Emulsion Polymerization Method.

Due to the increasing difficulty of drilling in the later stages of oil and gas field development, the development of micro-pores and micro-fractures is becoming common. Conventional plugging agents have relatively large particle sizes. So, choosing the appropriate plugging agent can prevent leakages. Using the inverse emulsion polymerization method, acrylamide, 2-acrylamide-2-methylpropane sulfonic acid and acrylic acid were selected to be the main reaction monomers, N,N'-methylenebisacrylamide was used as a crosslinking agent, sorbitan monostearate and polyoxyethylene sorbitan anhydride monostearate were used as emulsifiers, and 2,2'-azobis(2-methylpropionamidine) dihydrochloride was used as the initiator to synthesize a nano-scale plugging agent for oil-based drilling fluid. The plugging agent was characterized using infrared spectroscopy, scanning electron microscopy, and thermogravimetry analysis. The results showed that the plugging agent is spherical and uniform in size, with particles being in the submicron range. Additionally, it exhibited strong temperature resistance. Finally, the performance of the plugging agent was evaluated via experiments conducted under normal temperature and pressure, high-temperature and high-pressure, and core-plugging conditions. After adding the plugging agent to the oil-based drilling fluid, the basic rheological properties of the oil-based drilling fluid were not significantly affected. Furthermore, the filtration loss was significantly reduced under normal temperature and pressure, as well as under high-temperature and high-pressure conditions, after aging. When the plugging agent with 3% concentration was added, the reduction rate of pore core permeability reached 96.04%. Therefore, the plugging agent for the oil-based drilling fluid can effectively improve the wellbore stability and has a promising potential for field applications.

Effect of aggregation behavior on microplastic removal by magnetic Fe3O4 nanoparticles.

Magnetic nanotechnologies have been shown to be an efficient approach to the reduction of microplastic (MP) pollution in aquatic environments. However, uncertainties remain regarding the relationship between particle stability and MP removal under varying water conditions, hindering the practical application of magnetic nanotechnologies for MP removal. Herein, the influence of particle aggregation behavior on nano-scale MP removal by Fe3O4 nanoparticles (FNPs) was investigated, by monitoring dynamic light scattering parameters and analyzing the microstructures of particle aggregates. Results showed that 83.1 %-92.9 % of MPs could be removed by FNPs within 1 h, and MP removal exhibited a high degree of Pearson correlation (R = 0.95; P = 0.04) with particle aggregation behavior mediated by the FNPs dosage. Furthermore, pH-dependent electrostatic interactions significantly influenced particle aggregation behavior and the removal of MPs. Under pH <6.7 conditions, electrostatic attraction between electropositive FNPs and electronegative MPs led to charge neutralization-induced aggregation and efficient removal MP performance. Under increasingly saline conditions, compression of the electrical double layer enhanced the self-aggregation behavior of MPs, weakening the electrostatic repulsion between FNPs and MPs under alkaline conditions. Therefore, salinity improved the MP removal efficiency, especially under alkaline conditions, with MP removal increasing from 4.47 % to 55.1 % when the mass fraction of NaCl was increased from 0 % to 1 %. These findings further our understanding of the effect of aggregation behavior on MP removal by FNPs and highlight the potential for magnetic nanotechnology application in the removal of nano-scale MPs from aquatic environments, while also providing valuable insights for the design of FNP-based materials.

Effect of Annealing Temperature on the Microstructure and Mechanical Properties of CoCrFeNiNb0.2Mo0.2 High Entropy Alloy.

Strengthening the CoCrFeNi high entropy alloy with a face-center cubic structure has become a research prospect in the last decade. Alloying with double elements, Nb and Mo, is an effective method. In this paper, to further enhance the strength of the Nb and Mo contained high entropy alloy, CoCrFeNiNb0.2Mo0.2 was annealing treated at different temperatures for 24 h. As a result, a new kind of Cr2Nb type nano-scale precipitate with a hexagonal close-packed structure was formed, which is semi-coherent with the matrix. Moreover, by adjusting the annealing temperature, the precipitate was tailored with a considerable quantity and fine size. The best overall mechanical properties were achieved in the alloy annealed at 700 °C. The yield strength, ultimate tensile strength, and elongation are 727 MPa, 1.05 GPa, and 8.38%, respectively. The fracture mode of the annealed alloy is a mixture of cleavage and necking-featured ductile fracture. The approach employed in this study offers a theoretical foundation for enhancing the mechanical properties of face-centered cubic high entropy alloys via annealing treatment.

Solution-processable, robust and sustainable cooler via nano-structured engineering.

Passive daytime radiative cooling (PDRC) materials simultaneously featuring aesthetic and safety distinctions demonstrate versatile applications beyond cooling buildings, while the integrated advantages of high strength, morphological reconfigurability, and sustainability remain challenging for the conventional PDRC materials. Herein, we designed a robust, custom-shaped and eco-friendly cooler via a scalable solution-processable strategy, involving the nano-scale assembly of nano cellulose (NC) and inorganic nanoparticle (e.g., ZrO2, SiO2, BaSO4, and hydroxyapatite). The robust cooler shows an interesting "brick-and-mortar" structure, where the NC constructs interwoven framework (as brick structure) and the inorganic nanoparticle uniformly locates in the skeleton (as mortar structure), collectively contributing to high mechanical strength (>80 MPa) and flexibility. In addition, the structural and chemical distinctions enable our cooler to show a high solar reflectance (>96 %) and mid-infrared emissivity (>0.9), demonstrating a sub-ambient average temperature drop of 8.8 °C in long-term outdoor environments. The high-performance cooler with robustness, scalability and environmental friendliness, serves as a competitive participant toward the advanced PDRC materials in our low-carbon society.

Spectacular role of epididymis and bio-active cargo of nano-scale exosome in sperm maturation: A review.

The epididymis is responsible for post-testicular sperm maturation as it provides a favorable environment for spermatozoa to gain the ability for movement and fertilization. The recent evidence has shown that, the spermatozoa are vulnerable to dynamic variations driven by various cellular exposure mechanisms mediated by epididymosomes. Exosomes provide new insight into a mechanism of intercellular communication because they provide direct evidence for the transfer of several important bio-active cargo elements (proteins, lipid, DNA, mRNA, microRNA, circular RNA, long noncoding RNA) between epididymis and spermatozoa. In broad sense, proteomic analysis of exosomes from epididymis indicates number of proteins that are involved in sperm motility, acrosomal reaction, prevent pre-mature sperm capacitation and male infertility. Pinpointing, how reproductive disorders are associated with bio-active cargo elements of nano-scale exosome in the male reproductive tract. Therefore, the current review presents evidence regarding the distinctive characteristics and functions of nano-scale exosome in the male reproductive tract in both pathological and physiological developments, and argue that these vesicles serve as an important regulator of male reproduction, fertility, and disease susceptibility.

Cadmium-induced impairment of spermatozoa development by reducing exosomal-MVBs secretion: a novel pathway.

Cadmium is a heavy environmental pollutant that presents a high risk to male-fertility and targets the different cellular and steroidogenic supporting germ cells networks during spermatogenesis. However, the mechanism accounting for its toxicity in multivesicular bodies (MVBs) biogenesis, and exosomal secretion associated with spermatozoa remains obscure. In the current study, the light and electron microscopy revealed that, the Sertoli cells perform a dynamic role with secretion of well-developed early endosomes (Ee) and MVBs pathway associated with spermatozoa during spermatogenesis. In addition, some apical blebs containing nano-scale exosomes located on the cell surface and after fragmentation nano-scale exosomes were directly linked with spermatozoa in the luminal compartment of seminiferous tubules, indicating normal spermatogenesis. Controversially, the cadmium treated group showed limited and deformed spermatozoa with damaging acromion process and mid-peace, and the cytoplasmic vacuolization of spermatids. After cadmium treatment, there is very limited biogenesis of MVBs inside the cytoplasm of Sertoli cells, and no obvious secretions of nano-scale exosomes interacted with spermatozoa. Interestingly, the cadmium treated group demonstrated relatively higher formation of autophagosomes and autolysosome, and the autophagosomes were enveloped by MVBs that later formed the amphisome which degraded by lysosomes, indicating the hypo-spermatogenesis. Moreover, cadmium declined the exosomal protein cluster of differentiation (CD63) and increased the autophagy-related proteins microtubule-associated light chain (LC3), sequestosome 1 (P62) and lysosomal-associated membrane protein 2 (LAMP2) expression level were confirmed by Western blotting. These results provide rich information regarding how cadmium is capable of triggering impaired spermatozoa development during spermatogenesis by reduction of MVBs pathway through high activation of autophagic pathway. This study explores the toxicant effect of cadmium on nano-scale exosomes secretion interacting with spermatozoa.

Electrochemical Oxidation to Fabricate Micro-Nano-Scale Surface Wrinkling of Liquid Metals.

Constructing wrinkled structures on the surface of materials to obtain new functions has broad application prospects. Here a generalized method is reported to fabricate multi-scale and diverse-dimensional oxide wrinkles on liquid metal surfaces by an electrochemical anodization method. The oxide film on the surface of the liquid metal is successfully thickened to hundreds of nanometers by electrochemical anodization, and then the micro-wrinkles with height differences of several hundred nanometers are obtained by the growth stress. It is succeeded in altering the distribution of growth stress by changing the substrate geometry to induce different wrinkle morphologies, such as one-dimensional striped wrinkles and two-dimensional labyrinth wrinkles. Further, radial wrinkles are obtained under the hoop stress induced by the difference in surface tensions. These hierarchical wrinkles of different scales can exist on the liquid metal surface simultaneously. Surface wrinkles of liquid metal may have potential applications in the future for flexible electronics, sensors, displays, and so on.

MALDI mass spectrometry imaging shows a gradual change in the proteome landscape during mouse ovarian folliculogenesis.

Our knowledge regarding the role proteins play in the mutual relationship among oocytes, surrounding follicle cells, stroma, and the vascular network inside the ovary is still poor and obtaining insights into this context would significantly aid our understanding of folliculogenesis. Here, we describe a spatial proteomics approach to characterize the proteome of individual follicles at different growth stages in a whole prepubertal 25-day-old mouse ovary. A total of 401 proteins were identified by nano-scale liquid chromatography-electrospray ionization-tandem mass spectrometry (nLC-ESI-MS/MS), 69 with a known function in ovary biology, as demonstrated by earlier proteomics studies. Enrichment analysis highlighted significant KEGG and Reactome pathways, with apoptosis, developmental biology, PI3K-Akt, epigenetic regulation of gene expression, and extracellular matrix organization being well represented. Then, correlating these data with the spatial information provided by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) on 276 follicles enabled the protein profiles of single follicle types to be mapped within their native context, highlighting 94 proteins that were detected throughout the secondary to the pre-ovulatory transition. Statistical analyses identified a group of 37 proteins that showed a gradual quantitative change during follicle differentiation, comprising 10 with a known role in follicle growth (NUMA1, TPM2), oocyte germinal vesicle-to-metaphase II transition (SFPQ, ACTBL, MARCS, NUCL), ovulation (GELS, CO1A2), and preimplantation development (TIF1B, KHDC3). The proteome landscape identified includes molecules of known function in the ovary, but also those whose specific role is emerging. Altogether, this work demonstrates the utility of performing spatial proteomics in the context of the ovary and offers sound bases for more in-depth investigations that aim to further unravel its spatial proteome.

Effect of Strain Rate on Nano-Scale Mechanical Behavior of A-Plane (112¯0) ZnO Single Crystal by Nanoindentation.

In this study, nanoindentation tests at three different strain rates within 100 nm indentation depth were conducted on an a-plane (112¯0) ZnO single crystal to investigate the effect of strain rate on its nano-scale mechanical behavior. The load-indentation-depth curves, pop-in events, hardness and Young's moduli of an a-plane (112¯0) ZnO single crystal at different strain rates were investigated at the nano-scale level. The results indicated that, with the indentation depth increasing, the load increased gradually at each maximum indentation depth, hma, during the loading process. A distinct pop-in event occurred on each loading curve except that corresponding to the hmax of 10 nm. The applied load at the same indentation depth increased with the increasing strain rate during the nanoindentation of the a-plane (112¯0) ZnO single crystal. The higher strain rate deferred the pop-in event to a higher load and deeper indentation depth, and made the pop-in extension width larger. The hardness showed reverse indentation size effect (ISE) before the pop-in, and exhibited normal ISE after the pop-in. Both the hardness and the Young's modulus of the a-plane (112¯0) ZnO single crystal increased with the increasing strain rate, exhibiting the positive strain-rate sensitivity.

Atomistic-Scale Energetic Heterogeneity on a Membrane Surface.

Knowing the energetic topology of a surface is important, especially with regard to membrane fouling. In this study, molecular computations were carried out to determine the energetic topology of a polyvinylidene fluoride (PVDF) membrane with different surface wettability and three representative probe molecules (namely argon, carbon dioxide and water) of different sizes and natures. Among the probe molecules, water has the strongest interaction with the PVDF surface, followed by carbon dioxide and then argon. Argon, which only has van der Waals interactions with PVDF, is a good probing molecule to identify crevices and the molecular profile of a surface. Carbon dioxide, which is the largest probing molecule and does not have dipole moment, exhibits similar van der Waals and electrostatic interactions. As for water, the dominant attractive interactions are electrostatics with fluorine atoms of the intrinsically hydrophobic PVDF membrane, but the electrostatic interactions are much stronger for the hydroxyl and carboxyl groups on the hydrophilic PVDF due to strong dipole moment. PVDF only becomes hydrophilic when the interaction energy is approximately doubled when grafted with hydroxyl and carboxyl groups. The energetic heterogeneity and the effect of different probe molecules revealed here are expected to be valuable in guiding membrane modifications to mitigate fouling.

Resveratrol in breast cancer treatment: from cellular effects to molecular mechanisms of action.

Breast cancer (BC) is one of the most common cancers in females and is responsible for the highest cancer-related deaths following lung cancer. The complex tumor microenvironment and the aggressive behavior, heterogenous nature, high proliferation rate, and ability to resist treatment are the most well-known features of BC. Accordingly, it is critical to find an effective therapeutic agent to overcome these deleterious features of BC. Resveratrol (RES) is a polyphenol and can be found in common foods, such as pistachios, peanuts, bilberries, blueberries, and grapes. It has been used as a therapeutic agent for various diseases, such as diabetes, cardiovascular diseases, inflammation, and cancer. The anticancer mechanisms of RES in regard to breast cancer include the inhibition of cell proliferation, and reduction of cell viability, invasion, and metastasis. In addition, the synergistic effects of RES in combination with other chemotherapeutic agents, such as docetaxel, paclitaxel, cisplatin, and/or doxorubicin may contribute to enhancing the anticancer properties of RES on BC cells. Although, it demonstrates promising therapeutic features, the low water solubility of RES limits its use, suggesting the use of delivery systems to improve its bioavailability. Several types of nano drug delivery systems have therefore been introduced as good candidates for RES delivery. Due to RES's promising potential as a chemopreventive and chemotherapeutic agent for BC, this review aims to explore the anticancer mechanisms of RES using the most up to date research and addresses the effects of using nanomaterials as delivery systems to improve the anticancer properties of RES.

The Release of Non-Extractable Ferulic Acid from Cereal By-Products by Enzyme-Assisted Hydrolysis for Possible Utilization in Green Synthesis of Silver Nanoparticles.

The present work was undertaken to elucidate the potential contribution of biosynthetically produced ferulic acid (FA) via enzymatic hydrolysis (EH) of rye bran (RB) to the formation of silver nanoparticles (AgNPs) during green synthesis. An analytical approach accomplished by multiple reaction monitoring (MRM) using triple quadrupole mass selective detection (HPLC-ESI-TQ-MS/MS) of the obtained hydrolysate revealed a relative abundance of two isomeric forms of FA, i.e., trans-FA (t-FA) and trans-iso-FA (t-iso-FA). Further analysis utilizing high-performance liquid chromatography with refractive index (HPLC-RID) detection confirmed the effectiveness of RB EH, indicating the presence of cellulose and hemicellulose degradation products in the hydrolysate, i.e., xylose, arabinose, and glucose. The purification process by solid-phase extraction with styrene-divinylbenzene-based reversed-phase sorbent ensured up to 116.02 and 126.21 mg g-1 of t-FA and t-iso-FA in the final eluate fraction, respectively. In the green synthesis of AgNPs using synthetic t-FA, the formation of NPs with an average size of 56.8 nm was confirmed by scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) techniques. The inclusion of polyvinylpyrrolidone (PVP-40) in the composition of NPs during synthesis favorably affected the morphological features, i.e., the size and shape of AgNPs, in which as big as 22.4 nm NPs were engineered. Meanwhile, nearly homogeneous round-shaped AgNPs with an average size of 16.5 nm were engineered using biosynthetically produced a mixture of t-FA and t-iso-FA and PVP-40 as a capping agent. The antimicrobial activity of AgNPs against Gram-positive and Gram-negative bacteria, including Pseudomonas aeruginosa, E. coli, Enterococcus faecalis, Bacillus subtilis, and Staphylococcus aureus was confirmed by the disk diffusion method and additionally supported by values of minimum inhibitory (MIC) and bactericidal (MBC) concentrations. Given the need to reduce problems of environmental pollution with cereal processing by-products, this study demonstrated a technological solution of RB rational use in the sustainable production of AgNPs during green synthesis. The AgNPs can be considered as active pharmaceutical ingredients (APIs) to be used for developing new antimicrobial agents and modifying therapies in treating multi-drug resistant (MDR) pathogens.

Nickel bioaccessibility in soils with high geochemical background and anthropogenic contamination.

Abnormally high concentrations of metals including nickel (Ni) in soils result from high geochemical background (HB) or anthropogenic contamination (AC). Metal bioaccessibility in AC-soils has been extensively explored, but studies in HB-soils are limited. This study examined the Ni bioaccessibility in basalt and black shale derived HB-soils, with AC-soils and soils without contamination (CT) being used for comparison. Although HB- and AC-soils had similar Ni levels (123 ± 43.0 vs 155 ± 84.7 mg kg-1), their Ni bioaccessibility based on the gastric phase of the Solubility Bioaccessibility Research Consortium (SBRC) in vitro assay was different. Nickel bioaccessibility in HB-soils was 6.42 ± 3.78%, 2-times lower than the CT-soils (12.0 ± 9.71%) and 6-times lower than that in AC-soils (42.6 ± 16.3%). Based on the sequential extraction, a much higher residual Ni fractionation in HB-soils than that in CT- and AC-soils was observed (81.9 ± 9.52% vs 68.6 ± 9.46% and 38.7 ± 16.0%). Further, correlation analysis indicate that the available Ni (exchangeable + carbonate-bound + Fe/Mn hydroxide-bound) was highly correlated with Ni bioaccessibility, which was also related to the organic carbon content in soils. The difference in co-localization between Ni and other elements (Fe, Mn and Ca) from high-resolution NanoSIMS analysis provided additional explanation for Ni bioaccessibility. In short, based on the large difference in Ni bioaccessibility in geochemical background and anthropogenic contaminated soils, it is important to base contamination sources for proper risk assessment of Ni-contaminated soils.