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1.
J Environ Sci (China) ; 148: 38-45, 2025 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-39095173

RESUMEN

Nitrate (NO3-) is a widespread pollutant in high-salt wastewater and causes serious harm to human health. Although electrochemical removal of nitrate has been demonstrated to be a promising treatment method, the development of low-cost electro-catalysts is still challenging. In this work, a phosphate modified iron (P-Fe) cathode was prepared for electrochemical removal of nitrate in high-salt wastewater. The phosphate modification greatly improved the activity of iron, and the removal rate of nitrate on P-Fe was three times higher than that on Fe electrode. Further experiments and density functional theory (DFT) calculations demonstrated that the modification of phosphoric acid improved the stability and the activity of the zero-valent iron electrode effectively for NO3- removal. The nitrate was firstly electrochemically reduced to ammonium, and then reacted with the anodic generated hypochlorite to N2. In this study, a strategy was developed to improve the activity and stability of metal electrode for NO3- removal, which opened up a new field for the efficient reduction of NO3- removal by metal electrode materials.


Asunto(s)
Electrodos , Hierro , Nitratos , Fosfatos , Eliminación de Residuos Líquidos , Aguas Residuales , Contaminantes Químicos del Agua , Aguas Residuales/química , Nitratos/química , Hierro/química , Fosfatos/química , Contaminantes Químicos del Agua/química , Eliminación de Residuos Líquidos/métodos , Técnicas Electroquímicas/métodos
2.
Methods Mol Biol ; 2852: 123-134, 2025.
Artículo en Inglés | MEDLINE | ID: mdl-39235740

RESUMEN

Properly using controllable atmospheric containers can facilitate investigations of the survival abilities and physiological states of key and emerging-foodborne pathogens under recreated applicable food processing environmental conditions. Notably, saturated salt solutions can efficiently control relative humidity in airtight containers. This chapter describes a practical experimental setup, with necessary prerequisites for exposing foodborne pathogens to simulated and relevant food processing environmental conditions. Subsequent analyses for studying cell physiology will also be suggested.


Asunto(s)
Manipulación de Alimentos , Microbiología de Alimentos , Manipulación de Alimentos/métodos , Enfermedades Transmitidas por los Alimentos/microbiología , Viabilidad Microbiana , Bacterias/crecimiento & desarrollo , Humanos
3.
Artículo en Inglés | MEDLINE | ID: mdl-39254890

RESUMEN

INTRODUCTION: Patients with alopecia areata (AA) may have received several therapies for management of AA during their lives. In the ALLEGRO phase 2b/3 (NCT03732807) study, the oral JAK3/TEC family kinase inhibitor ritlecitinib demonstrated efficacy and an acceptable safety profile in patients aged ≥ 12 years with AA and ≥ 50% scalp hair loss. This post hoc analysis investigated associations between prior use of AA therapies and Severity of Alopecia Tool (SALT) responses in patients receiving ritlecitinib for AA. METHODS: Patients receiving ritlecitinib 30 mg or 50 mg once daily with or without an initial 4-week 200-mg daily loading dose were grouped by previous exposure to AA treatments, including topicals, intralesional corticosteroids (ILCS), topical immunotherapy, and systemic immunosuppressants or any prior AA treatment. Multivariable logistic regression analyses evaluated the association between response based on a SALT score of ≤ 20 and any prior treatment for AA at weeks 24 and 48. RESULTS: Of 522 patients, 360 (69.0%) had previous exposure to any AA treatment. At Week 24, SALT ≤ 20 response was positively associated with prior use of ILCS (odds ratio [OR], 2.12; 95% confidence interval [CI], 1.23-3.65; P < 0.05) and negatively associated with prior use of systemic immunosuppressants (OR 0.50; 95% CI 0.28-0.88; P < 0.05). Prior use of topicals or topical immunotherapy was not associated with SALT ≤ 20 response at Week 24. By Week 48, no association was identified between SALT ≤ 20 response and prior use of topicals, ILCS, topical immunosuppressants, or systemic immunosuppressants (all P > 0.05). Previous exposure to any AA therapy was not associated with SALT ≤ 20 response at weeks 24 or 48 (all P > 0.05). CONCLUSIONS: Prior AA treatment history had no effect on longer-term treatment response to ritlecitinib. TRIAL REGISTRATION NUMBER: NCT03732807.

4.
Heliyon ; 10(16): e35868, 2024 Aug 30.
Artículo en Inglés | MEDLINE | ID: mdl-39220906

RESUMEN

Twenty-five promising salt-tolerant mulberry germplasms from different Morus species were evaluated for growth under varying salinity levels (10.20-25.60 dS m-1) typical of the coastal regions in South 24 Parganas, West Bengal. Evaluations were conducted using in vitro axillary bud culture and field experiments under natural conditions to identify superior salt-tolerant germplasms. Soil sample analysis revealed significant variation in salinity levels (34.37-17.09 dS m-1) across different areas, with the highest in Kultali and the lowest in Canning I and II. Among the 25 germplasms, 6 were identified as highly salt-tolerant, 6 as moderately high salt-tolerant, 11 as salt-tolerant, and 2 as salt-sensitive. Survivability rate and root length were found to have the highest correlation with salt tolerance during early development. The six highly salt-tolerant germplasms, including English Black, Kolitha-3, C776, Rotundiloba, BC259, and S1 were further tested in field trials. English Black showed the highest survivability rate of 69.2 % in soil salinity of 18-20 dS m-1. Results from in vitro and field trials were consistent, with a strong positive correlation between survivability rate and root length. This study establishes an effective method for evaluating salt tolerance in mulberry, providing a foundation for more efficient assessments.

5.
Environ Res ; : 119899, 2024 Aug 31.
Artículo en Inglés | MEDLINE | ID: mdl-39222732

RESUMEN

The aerobic composting process is extensively utilized to manage kitchen waste. Nonetheless, the variability in the quality of compost derived from engineering practices which significantly hinders its broader industrial application. This work investigated the final products of kitchen waste compost at multiple industrial-scale treatment facilities utilizing three distinct aerobic composting processes in a bid to explore key factors affecting compost quality. The quality evaluation was based on technical parameters like seed germination index (GI), and limiting factors such as heavy metal content. The results showed that most of the compost products failed to meet the established standards, with GI being the primary limiting indicator. Furthermore, maturity assessments suggested that compost with low GI exhibited reduced humification could not be recommended for agricultural use. The investigation delved into the primary determinants of GI, focusing on risk factors such as the oil and salt of kitchen waste, and the microbial community of the humification driving forces. The results indicated that products with low GI had higher oil and salt content and a relatively simple microbial community. A thorough analysis suggested that excessive levels oil and salt were potential influencing factors on GI, as they stimulated the activity of acid-producing bacteria like Lactobacillus, suppressed the activity of humification-promoting bacteria such as Actinomarinales, and influenced the decomposition and humification processes of organic matter and total nitrogen, thereby affecting product quality. The findings provide valuable insights for improving kitchen waste compost products for agricultural applications.

6.
Ecol Appl ; : e3021, 2024 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-39219158

RESUMEN

Shrinking saline lakes provide irreplaceable habitat for waterbird species globally. Disentangling the effects of wetland habitat loss from other drivers of waterbird population dynamics is critical for protecting these species in the face of unprecedented changes to saline lake ecosystems, ideally through decision-making frameworks that identify effective management options and their potential outcomes. Here, we develop a framework to assess the effects of hypothesized population drivers and identify potential future outcomes of plausible management scenarios on a saline lake-reliant waterbird species. We use 36 years of monitoring data to quantify the effects of environmental conditions on the population size of a regionally important breeding colony of American white pelicans (Pelecanus erythrorhynchos) at Great Salt Lake, Utah, US, then forecast colony abundance under various management scenarios. We found that low lake levels, which allow terrestrial predators access to the colony, are probable drivers of recent colony declines. Without local management efforts, we predicted colony abundance could likely decline approximately 37.3% by 2040, although recent colony observations suggest population declines may be more extreme than predicted. Results from our population projection scenarios suggested that proactive approaches to preventing predator colony access and reversing saline lake declines are crucial for the persistence of the Great Salt Lake pelican colony. Increasing wetland habitat and preventing predator access to the colony together provided the most effective protection, increasing abundance 145.4% above projections where no management actions are taken, according to our population projection scenarios. Given the importance of water levels to the persistence of island-nesting colonial species, proactive approaches to reversing saline lake declines could likely benefit pelicans as well as other avian species reliant on these unique ecosystems.

7.
J Plant Physiol ; 303: 154336, 2024 Sep 08.
Artículo en Inglés | MEDLINE | ID: mdl-39260050

RESUMEN

Ethylene (ET) and gibberellins (GAs) play key roles in controlling the biotic and abiotic interactions between plants and environment. To gain insights about the role of ET and GAs interactions in the mycorrization and response to salinity of tomato (Solanum lycopersicum L.) plants, the ET-insensitive (Never-ripe, Nr), and the ET-overproducer (Epinastic, Epi) mutants and their wild type cv. Micro-Tom (MT), were inoculated or not with the arbuscular mycorrhizal fungi (AMF) Rhizophagus irregularis and exposed to control (0 mM NaCl) and salinity (100 mM NaCl) conditions, with and without gibberellic acid (10-6 M GA3) application during four weeks. Exogenous GA3 enhanced plant growth irrespective of the genotype, AMF, and salinity conditions, while an additional effect on growth by AMF was only found in the ET-overproducer (Epi) mutant under control and salinity conditions. Epi almost doubled the AMF colonization level under both conditions but was the most affected by salinity and GA3 application compared to MT and Nr. In contrast, Nr registered the lowest AMF colonization level, but GA3 produced a positive effect, particularly under salinity, with the highest leaf growth recovery. Foliar GA3 application increased the endogenous concentration of GA1, GA3, and total GAs, more intensively in AMF-Epi plants, where induced levels of the ET-precursor ACC were also found. Interestingly, GA4 which is associated with AMF colonization, registered the strongest genotype x GA x AMF × salinity interactions. The different growth responses in relation to those interactions are discussed.

8.
Plant Physiol Biochem ; 216: 109098, 2024 Sep 07.
Artículo en Inglés | MEDLINE | ID: mdl-39260261

RESUMEN

The transcription factor Ethylene Responsive Factor (ERF) is crucial for responding to various environmental stressors. Proteins containing the ERF-associated amphiphilic repression (EAR) motif often inhibit gene expression. However, the functions of LkERF, an EAR motif-containing protein from Larix kaempferi, especially in reactive oxygen species (ROS) homeostasis, are not well understood. In the present research, we introduce a novel transcription factor, LkERF6, which contains an EAR motif and positively regulates gene expression, thereby enhancing drought and salt tolerance in tobacco. LkERF6 is classified within the ERF-B1 subfamily due to its conserved AP2/ERF domain and EAR motif. Subcellular localization assays demonstrated LkERF6 is primarily localized in the nucleus. Further analysis revealed that LkERF6 interacts with GCC and DRE elements and is significantly induced by NaCl and PEG6000. Moreover, LkERF6 transgenic tobacco plants exhibit lower ROS accumulation and higher levels of antioxidant enzyme activities. Additionally, correlation analysis identified a strong association between LkERF6 and three genes: LkSOD, LkCCS, and LkCAT. Y1H, EMAS, and DLR assays confirmed that LkERF6 directly interacts with the promoters of these genes through GCC-box and DRE-box to activate their expression. These findings shed new light on the function of EAR motif-containing transcription factors and highlight LkERF6's crucial role in enhancing abiotic stress resistance by activating multiple ROS clearance genes.

9.
New Phytol ; 2024 Sep 12.
Artículo en Inglés | MEDLINE | ID: mdl-39262232

RESUMEN

Salvia miltiorrhiza holds significant importance in traditional Chinese medicine. Stress-associated proteins (SAP), identified by A20/AN1 zinc finger structural domains, play crucial roles in regulating plant growth, development, resistance to biotic and abiotic stress, and hormone responses. Herein, we conducted a genome-wide identification of the SAP gene family in S. miltiorrhiza. The expression analysis revealed a significant upregulation of SmSAP4 under methyl jasmonate (MeJA) and salt stress. Overexpressing SmSAP4 in S. miltiorrhiza hairy roots increased tanshinones content while decreasing salvianolic acids content, while RNAi-silencing SmSAP4 had the opposite effect. SmSAP4 overexpression in both Arabidopsis thaliana and S. miltiorrhiza hairy roots decreased their salt stress tolerance, accompanied by increased activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), and a hindered ability to maintain the Na+ : K+ ratio. Further investigations demonstrated that MeJA alleviated the inhibitory effect of SmJAZ3 on SmSAP4 activation by SmbHLH37 and SmERF73. However, MeJA did not affect the inhibition of SmSAP4 activation by SmJAZ8 through SmbHLH37. In summary, our research reveals that SmSAP4 negatively regulates the accumulation of salvianic acid through the SmJAZs-SmbHLH37/SmERF73-SmSAP4 module and positively impacting the accumulation of tanshinones. Additionally, it functions as a negative regulator under salt stress.

10.
Heliyon ; 10(17): e36762, 2024 Sep 15.
Artículo en Inglés | MEDLINE | ID: mdl-39263153

RESUMEN

To investigate the effect of poly-γ-glutamic acid (γ-PGA) biopreparation on ameliorating coastal saline soil, three treatments were established: soil salt washed treatment (CK), soil salt washed with added γ-PGA (PGA), soil salt washed with added γ-PGA biopreparation (PGAB). This study determined the effects of γ-PGA on coastal saline soil by analyzing soil aggregate, soil evaporation, soil vertical water and salt distribution, and soil cation content, soil pH, soil nutrient content and soil microorganism quantity. Results showed that γ-PGA had an ameliorative effect on saline soil, with the PGAB treatment exhibiting the most pronounced ameliorative effect compared to CK. Adding PGAB reduced soil evaporation by 30.45 %, soil salt content by 27.91 %, meanwhile increasing plant height by 33.86 %, plant fresh weight by 98.54 %, soil aggregate diameter by 6.68 times, soil water content by 26.47 % (P < 0.05). Additionally, soil total nitrogen was increased by 50.0 % in PGAB treatment, and available nitrogen and phosphorus contents were increased by 1.68 times and 85.83 % (P < 0.05), respectively. Populations of soil-culturable bacteria and fungi of PGAB treatment increased by 65.96 % and 1.23 times, respectively (P < 0.05). After salt-washing process, adding PGAB improved soil physicochemical properties, which altered the ecological environment of rhizosphere soil and promoted plant growth. The results can provide a practical approach for ameliorating coastal saline soils.

11.
Tree Physiol ; 2024 Sep 04.
Artículo en Inglés | MEDLINE | ID: mdl-39231271

RESUMEN

None declared.Conflict of interestSoil salinization has become a global problem and high salt concentration in soil negatively affects plant growth. In our previous study, we found that overexpression of PsAMT1.2 from Populus simonii could improve the salt tolerance of poplar, but the physiological and molecular mechanism was not well understood. To explore the regulation pathway of PsAMT1.2 in salt tolerance, we investigated the morphological, physiological, and transcriptome differences between the PsAMT1.2 overexpression transgenic poplar and the wild type (WT) under salt stress. The PsAMT1.2 overexpression transgenic poplar showed better growth with increased net photosynthetic rate and higher chlorophyll content compared with WT under salt stress. The overexpression of PsAMT1.2 increased the catalase, superoxide dismutase, peroxidase, ascorbate peroxidase activities and therefore probably enhanced the reactive oxygen species clearance ability, which also reduced the degree of membrane lipid peroxidation under salt stress. Meanwhile, the PsAMT1.2 overexpression transgenic poplar maintained a relatively high K+/Na+ ratio under salt stress. RNA-seq analysis indicated that PsAMT1.2 might improve plant salt tolerance by regulating pathways related to the photosynthetic system, chloroplast structure, antioxidant activity, and anion transport. Among the 1056 differentially expressed genes, genes related to photosystemIand photosystemIIwere up-regulated and genes related to chloride channel protein-related were down-regulated. The result of the present study would provide new insight into regulation mechanism of PsAMT1.2 in improving salt tolerance of poplar.

12.
Oecologia ; 2024 Sep 04.
Artículo en Inglés | MEDLINE | ID: mdl-39231844

RESUMEN

Salt marshes represent a unique ecosystem at the marine-terrestrial boundary of shallow protected coastlines. Microarthropods form an essential component of soil food webs, but how they colonize new intertidal habitats is little understood. By establishing two experimental systems without animals, we investigated microarthropod colonization (1) at the seashore from the pioneer zone to the lower and upper salt marsh and (2) at the same tidal height on artificial islands 500 m from the seashore. Potential source populations of microarthropods in the respective zones were also investigated. Colonization of microarthropods after 5 years was consistently faster on the seashore than on the artificial islands. Collembola and Mesostigmata colonized all the zones both on the seashore and on the artificial islands, with colonization being faster in the upper salt marsh and in the pioneer zone than in the lower salt marsh. Oribatida colonized the new habitats on the seashore, but only little on the artificial islands. Variations in species composition were more pronounced between salt marsh zones than between experimental systems, indicating that local environmental conditions (i.e., inundation frequency) are more important for the assembly of microarthropod communities than the distance from source populations (i.e., dispersal processes). Variations in community body size of Oribatida and Mesostigmata indicated environmental filtering of traits, with smaller species suffering from frequent inundations. Notably, Mesostigmata most successfully colonized the new habitats across salt marsh zones on both systems. Overall, the results document major mechanisms of colonization of intertidal habitats by microarthropods with different life histories and feeding strategies.

13.
Talanta ; 281: 126751, 2024 Aug 30.
Artículo en Inglés | MEDLINE | ID: mdl-39232251

RESUMEN

Freshwater resources have been gradually salinized in recent years, dramatically impacting the ecosystem and human health. Therefore, it is necessary to detect the salinity of freshwater resources. However, traditional detection methods make it difficult to check the type and concentration of salt quickly and accurately in solution. This paper uses a portable near-infrared spectrometer to qualitatively discriminate and quantitatively predict the salt in the solution. The study was carried out by adding ten salts of NaCl, KCl, MgCl2, CaCl2, Na2CO3, K2CO3, CaCO3, Na2SO4, K2SO4, MgSO4 to 2 mL of deionized water to prepare a single salt solution (0.02 %-1.00 %) totaling 100 sets. It was found that the Support vector machine (SVM) model was only effective in discriminating the class of salt anions in the solution. The Partial least squares-discriminant analysis (PLS-DA) model, on the other hand, can effectively discriminate the classes of salt in solution, and the accuracies of the optimal model prediction set and the interactive validation set are 98.86 % and 99.66 %, respectively. Furthermore, the Partial least squares regression (PLSR) models can accurately predict the concentration of NaCl, KCl, MgCl2, CaCl2, Na2CO3, K2CO3, CaCO3, Na2SO4, K2SO4, MgSO4 salt solutions. The coefficients of determination R2 of their model interactive validation sets were 0.99, 0.99, 0.99, 0.97, 0.99, 0.99, 0.98, 0.99, 0.98, and 0.98, respectively. This study shows that NIRS can achieve rapid and accurate qualitative and quantitative detection of salts in solution, which provides technical support for the utilization of safe water resources.

14.
Food Res Int ; 194: 114880, 2024 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-39232518

RESUMEN

The diseases caused by excessive sodium intake derived from NaCl consumption have attracted widespread attention worldwide, and many researchers are committed to finding suitable ways to reduce sodium intake during the dietary process. Salt substitute is considered an effective way to reduce sodium intake by replacing all/part of NaCl in food without reducing the saltiness while minimizing the impact on the taste and acceptability of the food. Plant-derived natural ingredients are generally considered safe and reliable, and extensive research has shown that certain plant extracts or specific components are effective salt substitutes, which can also give food additional health benefits. However, these plant-derived salt substitutes (PSS) have not been systematically recognized by the public and have not been well adopted in the food industry. Therefore, a comprehensive review of PSS, including its material basis, flavor characteristics, and taste mechanism is helpful for a deeper understanding of PSS, accelerating its research and development, and promoting its application.


Asunto(s)
Cloruro de Sodio Dietético , Gusto , Humanos , Cloruro de Sodio Dietético/análisis , Extractos Vegetales
15.
Food Res Int ; 194: 114934, 2024 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-39232545

RESUMEN

Potassium citrate (KC) and potassium lactate (KL) are considered as salt replacers due to their saltiness, processing advantages, and health benefits. However, the obvious bitter taste associated with these compounds has limited their use in salt substitutes. Despite this challenge, little attention has been paid to improving their sensory properties. This study provided evidence that dietary polysaccharide carrageenan can effectively mask the bitterness of KC and KL by specifically binding K+ and forming double helix chains. A highly accurate prediction model was then established for the saltiness and bitterness of low-sodium salts using mixture design principles. Three low-sodium salt formulas containing different potassium salts (KC, KL, KCl), NaCl, and carrageenan were created based on the prediction model. These formulas exhibited favorable saltiness potencies (>0.85) without any noticeable odor, preserving the sensory characteristics of high-sodium food products like seasoning powder while significantly reducing their sodium content. This research provides a promising approach for the food industry to formulate alternative low-sodium products with substantially reduced sodium content, potentially contributing to decreased salt intake.


Asunto(s)
Gusto , Humanos , Cloruro de Sodio Dietético , Citrato de Potasio/química , Carragenina/química , Masculino , Femenino , Polisacáridos/química , Adulto , Compuestos de Potasio/química , Lactatos/química , Dieta Hiposódica
16.
Adv Sci (Weinh) ; : e2406451, 2024 Sep 04.
Artículo en Inglés | MEDLINE | ID: mdl-39232811

RESUMEN

Magnesium-based batteries have garnered significant attention due to their high energy density, excellent intrinsic safety, and low cost. However, the application process has been hindered by the high Mg2+ ions diffusion barrier in solid-state structures and solid-liquid interphase. To address this issue, a hybrid battery technology based on Mg anode and Fe-based Prussian Blue Analogue cathode doped with functional transition metal ions and N═O bonds is proposed. Combined multiscale experimental characterizations with theoretical calculations, the subtle lattice distortion can create an asymmetric diffusion path for the active ions, which enables reversible extraction with significantly reduced diffusion barriers achieved by synergistic doping. The optimized cathode exhibits a working potential of 2.3 V and an initial discharge capacity of 152 mAh g-1 at 50 mA g-1. With the preferred electrolyte combined with equivalent concentration [Mg2(µ-Cl)2(DME)4][AlCl4]2 and NaTFSI salt solution, the hybrid system demonstrates superior cycling performance over 200 cycles at a high current density of 200 mA g-1, maintaining ≈100% coulombic efficiency with superior ion dynamic. The findings are expected to be marked an important step in the further application of high-voltage cathodes for Mg-based hybrid batteries.

17.
Int J Biol Macromol ; 279(Pt 2): 135236, 2024 Aug 31.
Artículo en Inglés | MEDLINE | ID: mdl-39218171

RESUMEN

An intelligent pH response indicator film is an easy-to-use device for the real-time monitoring of meat freshness during transport and storage. Therefore, a novel pH-sensitive anthocyanin indicator film composed of polyvinyl alcohol-blueberry anthocyanin (BA)-2-hydroxypropyltrimethyl ammonium chloride chitosan (HACC) called PAH-2.0 with 1.2 mg/mL HACC to monitor meat freshness using HACC as the colorimetric enhancer has been developed. BA and HACC were mixed and immobilized in the polyvinyl alcohol matrix by hydrogen bonds, as confirmed via Fourier-transform infrared spectroscopy and X-ray diffraction. The inclusion of HACC improved the color stability and antioxidant and antibacterial properties of the PAH-2.0 film. When applied to pork for freshness monitoring at 4 °C, three freshness stages, including fresh, sub-fresh, and spoiled, could be clearly distinguished based on the color variations of the PAH-2.0 film. The distinct hierarchical color change from purple to blue-violet and finally to grayish-blue was highly correlated with the indicators of pork freshness: pH values, total volatile basic nitrogen, and total viable count. This study provides a simple and promising approach for fabricating meat freshness indicator films with high color recognition accuracy, thereby offering new possibilities for visual meat freshness monitoring.

18.
Rice (N Y) ; 17(1): 56, 2024 Sep 02.
Artículo en Inglés | MEDLINE | ID: mdl-39218839

RESUMEN

Seed vigor is a complex trait encompassing seed germination, seedling emergence, growth, seed longevity, and stress tolerance, all are crucial for direct seeding in rice. Here, we report that the AP2/ERF transcription factor OsRAV1 (RELATED TO ABI3 AND VP1) positively regulates seed germination, vigor, and salt tolerance. Additionally, OsRAV1 was differently expressed in embryo and endosperm, with the OsRAV1 localized in the nucleus. Transcriptomic analysis revealed that OsRAV1 modulates seed vigor through plant hormone signal transduction and phenylpropanoid biosynthesis during germination. Haplotype analysis showed that rice varieties carrying Hap3 displayed enhanced salt tolerance during seed germination. These findings suggest that OsRAV1 is a potential target in breeding rice varieties with high seed vigor suitable for direct seeding cultivation.

19.
BMC Plant Biol ; 24(1): 821, 2024 Sep 02.
Artículo en Inglés | MEDLINE | ID: mdl-39218905

RESUMEN

To address salinity stress in plants in an eco-friendly manner, this study investigated the potential effects of salinity-resistant bacteria isolated from saline agricultural soils on the growth of cucumber (Cucumis sativus, cv. Royal) seedlings. A greenhouse factorial experiment was conducted based on a completely randomized design (CRD) with two factors, salinity at four levels and five bacterial treatments, with three replications (n = 3). Initially, fifty bacterial isolates were screened for their salinity and drought tolerance, phosphate solubilization activity, along with production of auxin, siderophore and hydrogen cyanide. Isolates K4, K14, K15, and C8 exhibited the highest resistance to salinity and drought stresses in vitro. Isolates C8 and K15 demonstrated the highest auxin production capacity, generating 2.95 and 2.87 µg mL- 1, respectively, and also exhibited significant siderophore production capacities (by 14% and 11%). Additionally, isolates C8 and K14 displayed greater phosphate solubilization activities, by 184.64 and 122.11 µg mL- 1, respectively. The statistical analysis revealed that the selected four potent isolates significantly enhanced all growth parameters of cucumber plants grown under salinity stress conditions for six weeks. Plant height increased by 41%, fresh and dry weights by 35% and 7%, respectively, and the leaf area index by 85%. The most effective isolate, C8, was identified as Bacillus subtilis based on the 16 S rDNA amplicon sequencing. This study demonstrated that inoculating cucumber seedlings with halotolerant bacterial isolates, such as C8 (Bacillus subtilis), possessing substantial plant growth-promoting properties significantly alleviated salinity stress by enhancing plant growth parameters. These findings suggest a promising eco-friendly strategy for improving crop productivity in saline agricultural environments.


Asunto(s)
Cucumis sativus , Tolerancia a la Sal , Plantones , Cucumis sativus/microbiología , Cucumis sativus/crecimiento & desarrollo , Cucumis sativus/fisiología , Plantones/crecimiento & desarrollo , Plantones/microbiología , Plantones/fisiología , Bacterias/genética , Bacterias/aislamiento & purificación , Bacterias/metabolismo , Bacterias/clasificación , Microbiología del Suelo , Fosfatos/metabolismo , Sideróforos/metabolismo , Ácidos Indolacéticos/metabolismo , Salinidad , Sequías
20.
Heliyon ; 10(16): e35804, 2024 Aug 30.
Artículo en Inglés | MEDLINE | ID: mdl-39224350

RESUMEN

Resilience of the power system against natural disasters is a vital need for sustainable energy supply. As a result of global warming, lakes and rivers have dried out, resulting in dust hubs that threaten the normal operation of outdoor power system equipment. Unlike other events like hurricanes and blizzards, the impact of extreme salt dust on power system insulator failures and network resilience in affected areas remains unexamined. In this paper, to avoid power curtailment caused by insulators breakdown in electricity distribution networks, the resiliency assessment and enhancement of these networks against salt dust is investigated. Failure mechanism analysis of insulators and fragility curves extraction of them in face of salt pollution and relative humidity are done using mathematical modelling and experimental tests to extract the breakdown probability; Experimental tests are conducted in the High Voltage Laboratory, University of Tehran (HVLUT) and a novel method is proposed to extract 3-dimensional fragility curves of insulators. A Monte Carlo-based resiliency assessment method is then employed to obtain resiliency curve against the salt dust. Some suitable indicators are introduced for this purpose. In addition, several resiliency enhancement measures are proposed and ranked using a benefit to cost ratio (BCR) index. Numerical simulations are conducted on two real distribution feeders in a distribution grid around Urmia Salt Lake, Iran. Numerical results confirm the effectiveness and applicability of the proposed method.

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