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1.
J Vis Exp ; (183)2022 05 06.
Artículo en Inglés | MEDLINE | ID: mdl-35604171

RESUMEN

Since the historical experiment on the contraction of glycerinated muscle by adding ATP, which Szent-Györgyi demonstrated in the mid-20th century, in vitro reactivation of demembranated cells has been a traditional and potent way to examine cell motility. The fundamental advantage of this experimental method is that the composition of the reactivation solution may be easily changed. For example, a high-Ca2+ concentration environment that occurs only temporarily due to membrane excitation in vivo can be replicated in the lab. Eukaryotic cilia (a.k.a. flagella) are elaborate motility machinery whose regulatory mechanisms are still to be clarified. The unicellular green alga Chlamydomonas reinhardtii is an excellent model organism in the research field of cilia. The reactivation experiments using demembranated cell models of C. reinhardtii and their derivatives, such as demembranated axonemes of isolated cilia, have significantly contributed to understanding the molecular mechanisms of ciliary motility. Those experiments clarified that ATP energizes ciliary motility and that various cellular signals, including Ca2+, cAMP, and reactive oxygen species, modulate ciliary movements. The precise method for demembranation of C. reinhardtii cells and reactivation of the cell models is described here.


Asunto(s)
Chlamydomonas reinhardtii , Adenosina Trifosfato/metabolismo , Axonema/fisiología , Cilios/fisiología , Flagelos/fisiología
3.
Mol Biol Cell ; 32(20): ar12, 2021 10 01.
Artículo en Inglés | MEDLINE | ID: mdl-34319756

RESUMEN

Motile cilia of multiciliated epithelial cells undergo synchronized beating to produce fluid flow along the luminal surface of various organs. Each motile cilium consists of an axoneme and a basal body (BB), which are linked by a "transition zone" (TZ). The axoneme exhibits a characteristic 9+2 microtubule arrangement important for ciliary motion, but how this microtubule system is generated is not yet fully understood. Here we show that calmodulin-regulated spectrin-associated protein 3 (CAMSAP3), a protein that can stabilize the minus-end of a microtubule, concentrates at multiple sites of the cilium-BB complex, including the upper region of the TZ or the axonemal basal plate (BP) where the central pair of microtubules (CP) initiates. CAMSAP3 dysfunction resulted in loss of the CP and partial distortion of the BP, as well as the failure of multicilia to undergo synchronized beating. These findings suggest that CAMSAP3 plays pivotal roles in the formation or stabilization of the CP by localizing at the basal region of the axoneme and thereby supports the coordinated motion of multicilia in airway epithelial cells.


Asunto(s)
Cilios/metabolismo , Proteínas Asociadas a Microtúbulos/metabolismo , Microtúbulos/metabolismo , Citoesqueleto de Actina/metabolismo , Animales , Axonema/fisiología , Cuerpos Basales/fisiología , Células Epiteliales/metabolismo , Femenino , Masculino , Ratones , Ratones Endogámicos ICR , Ratones Transgénicos , Movimiento/fisiología , Tráquea/fisiología
4.
Elife ; 102021 04 26.
Artículo en Inglés | MEDLINE | ID: mdl-33899736

RESUMEN

We propose and discuss a model for flagellar mechanics in Euglena gracilis. We show that the peculiar non-planar shapes of its beating flagellum, dubbed 'spinning lasso', arise from the mechanical interactions between two of its inner components, namely, the axoneme and the paraflagellar rod. The spontaneous shape of the axoneme and the resting shape of the paraflagellar rod are incompatible. Thus, the complex non-planar configurations of the coupled system emerge as the energetically optimal compromise between the two antagonistic components. The model is able to reproduce the experimentally observed flagellar beats and the characteristic geometric signature of spinning lasso, namely, traveling waves of torsion with alternating sign along the length of the flagellum.


Asunto(s)
Axonema/fisiología , Cilios/fisiología , Flagelos/fisiología , Fenómenos Biomecánicos , Fenómenos Biofísicos , Euglena gracilis
5.
PLoS Genet ; 16(8): e1008691, 2020 08.
Artículo en Inglés | MEDLINE | ID: mdl-32764743

RESUMEN

Primary ciliary dyskinesia (PCD) is characterized by chronic airway disease, reduced fertility, and randomization of the left/right body axis. It is caused by defects of motile cilia and sperm flagella. We screened a cohort of affected individuals that lack an obvious axonemal defect for pathogenic variants using whole exome capture, next generation sequencing, and bioinformatic analysis assuming an autosomal recessive trait. We identified one subject with an apparently homozygous nonsense variant [(c.1762C>T), p.(Arg588*)] in the uncharacterized CFAP57 gene. Interestingly, the variant results in the skipping of exon 11 (58 amino acids), which may be due to disruption of an exonic splicing enhancer. In normal human nasal epithelial cells, CFAP57 localizes throughout the ciliary axoneme. Nasal cells from the PCD patient express a shorter, mutant version of CFAP57 and the protein is not incorporated into the axoneme. The missing 58 amino acids include portions of WD repeats that may be important for loading onto the intraflagellar transport (IFT) complexes for transport or docking onto the axoneme. A reduced beat frequency and an alteration in ciliary waveform was observed. Knockdown of CFAP57 in human tracheobronchial epithelial cells (hTECs) recapitulates these findings. Phylogenetic analysis showed that CFAP57 is highly conserved in organisms that assemble motile cilia. CFAP57 is allelic with the BOP2/IDA8/FAP57 gene identified previously in Chlamydomonas reinhardtii. Two independent, insertional fap57 Chlamydomonas mutant strains show reduced swimming velocity and altered waveforms. Tandem mass tag (TMT) mass spectroscopy shows that FAP57 is missing, and the "g" inner dyneins (DHC7 and DHC3) and the "d" inner dynein (DHC2) are reduced, but the FAP57 paralog FBB7 is increased. Together, our data identify a homozygous variant in CFAP57 that causes PCD that is likely due to a defect in the inner dynein arm assembly process.


Asunto(s)
Axonema/metabolismo , Trastornos de la Motilidad Ciliar/genética , Codón sin Sentido , Dineínas/metabolismo , Proteínas/genética , Células 3T3 , Adulto , Animales , Axonema/fisiología , Células Cultivadas , Chlamydomonas reinhardtii , Cilios/metabolismo , Cilios/fisiología , Trastornos de la Motilidad Ciliar/patología , Secuencia Conservada , Humanos , Masculino , Ratones , Proteínas Asociadas a Microtúbulos , Proteínas de Plantas/química , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Proteínas/química , Proteínas/metabolismo , Mucosa Respiratoria/metabolismo
6.
Exp Anim ; 69(4): 374-381, 2020 11 12.
Artículo en Inglés | MEDLINE | ID: mdl-32554934

RESUMEN

Infertility is a global health issue that affects 1 in 6 couples, with male factors contributing to 50% of cases. The flagellar axoneme is a motility apparatus of spermatozoa, and disruption of its structure or function could lead to male infertility. The axoneme consists of a "9+2" structure that contains a central pair of two singlet microtubules surrounded by nine doublet microtubules, in addition to several macromolecular complexes such as dynein arms, radial spokes, and nexin-dynein regulatory complexes. Molecular components of the flagellar axoneme are evolutionally conserved from unicellular flagellates to mammals, including mice. Although knockout (KO) mice have been generated to understand their function in the formation and motility regulation of sperm flagella, the majority of KO mice die before sexual maturation due to impaired ciliary motility, which makes it challenging to analyze mature spermatozoa. In this review, we introduce methods that have been used to overcome premature lethality, focusing on KO mouse lines of central pair components.


Asunto(s)
Axonema/fisiología , Cola del Espermatozoide/fisiología , Animales , Axonema/metabolismo , Axonema/ultraestructura , Dineínas/metabolismo , Infertilidad Masculina/etiología , Masculino , Ratones Noqueados , Proteínas Asociadas a Microtúbulos/metabolismo , Microtúbulos , Motilidad Espermática/fisiología , Cola del Espermatozoide/metabolismo , Cola del Espermatozoide/ultraestructura
7.
J Cell Biol ; 219(8)2020 09 07.
Artículo en Inglés | MEDLINE | ID: mdl-32543652

RESUMEN

Rcd4 is a poorly characterized Drosophila centriole component whose mammalian counterpart, PPP1R35, is suggested to function in centriole elongation and conversion to centrosomes. Here, we show that rcd4 mutants exhibit fewer centrioles, aberrant mitoses, and reduced basal bodies in sensory organs. Rcd4 interacts with the C-terminal part of Ana3, which loads onto the procentriole during interphase, ahead of Rcd4 and before mitosis. Accordingly, depletion of Ana3 prevents Rcd4 recruitment but not vice versa. We find that neither Ana3 nor Rcd4 participates directly in the mitotic conversion of centrioles to centrosomes, but both are required to load Ana1, which is essential for such conversion. Whereas ana3 mutants are male sterile, reflecting a requirement for Ana3 for centriole development in the male germ line, rcd4 mutants are fertile and have male germ line centrioles of normal length. Thus, Rcd4 is essential in somatic cells but is not absolutely required in spermatogenesis, indicating tissue-specific roles in centriole and basal body formation.


Asunto(s)
Centriolos/fisiología , Cilios/fisiología , Animales , Axonema/fisiología , Axonema/ultraestructura , Cilios/ultraestructura , Proteínas de Drosophila/fisiología , Femenino , Masculino , Mutación , Biogénesis de Organelos , Unión Proteica , Espermatogénesis/fisiología
8.
Cell Microbiol ; 22(3): e13121, 2020 03.
Artículo en Inglés | MEDLINE | ID: mdl-31634979

RESUMEN

Sexual development is an essential phase in the Plasmodium life cycle, where male gametogenesis is an unusual and extraordinarily rapid process. It produces 8 haploid motile microgametes, from a microgametocyte within 15 minutes. Its unique achievement lies in linking the assembly of 8 axonemes in the cytoplasm to the three rounds of intranuclear genome replication, forming motile microgametes, which are expelled in a process called exflagellation. Surprisingly little is known about the actors involved in these processes. We are interested in kinesins, molecular motors that could play potential roles in male gametogenesis. We have undertaken a functional characterization in Plasmodium berghei of kinesin-8B (PbKIN8B) expressed specifically in male gametocytes and gametes. By generating Pbkin8B-gfp parasites, we show that PbKIN8B is specifically expressed during male gametogenesis and is associated with the axoneme. We created a ΔPbkin8B knockout cell line and analysed the consequences of the absence of PbKIN8B on male gametogenesis. We show that the ability to produce sexually differentiated gametocytes is not affected in ΔPbkin8B parasites and that the 3 rounds of genome replication occur normally. Nevertheless, the development to free motile microgametes is halted and the life cycle is interrupted in vivo. Ultrastructural analysis revealed that intranuclear mitoses are unaffected whereas cytoplasmic microtubules, although assembled in doublets and elongated, fail to assemble in the normal axonemal '9+2' structure and become motile. Absence of a functional axoneme prevented microgamete assembly and release from the microgametocyte, severely reducing infection of the mosquito vector. This is the first functional study of a kinesin involved in male gametogenesis. These results reveal a previously unknown role for PbKIN8B in male gametogenesis, providing new insights into Plasmodium flagellar formation.


Asunto(s)
Axonema/fisiología , Cinesinas/genética , Cinesinas/fisiología , Plasmodium berghei/fisiología , Proteínas Protozoarias/fisiología , Animales , Culicidae/parasitología , Femenino , Técnicas de Inactivación de Genes , Genes Protozoarios , Estadios del Ciclo de Vida , Malaria/parasitología , Ratones , Mitosis , Modelos Animales , Mosquitos Vectores/parasitología , Organismos Modificados Genéticamente , Proteínas Protozoarias/genética
9.
PLoS Biol ; 17(7): e3000381, 2019 07.
Artículo en Inglés | MEDLINE | ID: mdl-31314751

RESUMEN

The primary cilium is a central signaling hub in cell proliferation and differentiation and is built and disassembled every cell cycle in many animal cells. Disassembly is critically important, as misregulation or delay of cilia loss leads to cell cycle defects. The physical means by which cilia are lost are poorly understood but are thought to involve resorption of ciliary components into the cell body. To investigate cilium loss in mammalian cells, we used live-cell imaging to comprehensively characterize individual events. The predominant mode of cilium loss was rapid deciliation, in which the membrane and axoneme of the cilium was shed from the cell. Gradual resorption was also observed, as well as events in which a period of gradual resorption was followed by rapid deciliation. Deciliation resulted in intact shed cilia that could be recovered from culture medium and contained both membrane and axoneme proteins. We modulated levels of katanin and intracellular calcium, two putative regulators of deciliation, and found that excess katanin promotes cilia loss by deciliation, independently of calcium. Together, these results suggest that mammalian ciliary loss involves a tunable decision between deciliation and resorption.


Asunto(s)
Axonema/fisiología , Diferenciación Celular/fisiología , Proliferación Celular/fisiología , Cilios/fisiología , Transducción de Señal/fisiología , Animales , Axonema/metabolismo , Calcio/metabolismo , Ciclo Celular/fisiología , Línea Celular , Cilios/metabolismo , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Katanina/genética , Katanina/metabolismo , Proteínas de la Membrana/genética , Proteínas de la Membrana/metabolismo , Ratones , Microscopía Confocal , Microscopía Fluorescente
10.
Biosystems ; 182: 42-51, 2019 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-31202860

RESUMEN

Cilia and flagella are cell organelles serving basic roles in cellular motility. Ciliary movement is performed by a sweeping-like repeated bending motion, which gives rise to a self-propagating "ciliary beat". The hallmark structure in cilia is the axoneme, a stable architecture of microtubule doublets. The motion of axoneme is powered by the axonemal dynein motor family powered by ATP hydrolysis. It is still unclear how the organized beat of cilium and flagella emerges from the combined action of hundreds of dynein molecules. It has been hypothesized that such coordination is mediated by mechanical stress due to transverse, radial or sliding deformations. The beating asymmetry is crucial for airway ciliary function and it requires tubulin glutamination a unique posttranslational modification of C-termini of constituent microtubules that is highly abundant in cilia and flagella. The exact role of tubulin glutamination in ciliary or flagellar function is still unclear. In this paper we analyze the role of calcium (Ca2+) ions based on the experimental evidence that the flagellar asymmetry can be increased due to the entry of extracellular Ca2+ through, for example, the nimodipine-sensitive pathway located in the flagella. We propose a new scenario based on the polyelectrolyte properties of cellular microtubules (MTs) such that dynamic influx of Ca2+ ions provides the initiation and synchronization of dynein sliding along microtubules. We also point out the possible interplay between tubulin polyglutaminated C-termini and localized pulses of Ca2+ ions along microtubules.


Asunto(s)
Algoritmos , Axonema/fisiología , Calcio/metabolismo , Cilios/fisiología , Flagelos/fisiología , Modelos Biológicos , Adenosina Trifosfato/metabolismo , Animales , Axonema/metabolismo , Cilios/metabolismo , Dineínas/metabolismo , Flagelos/metabolismo , Humanos , Iones/metabolismo , Microtúbulos/metabolismo , Movimiento , Transducción de Señal , Tubulina (Proteína)/metabolismo
11.
Open Biol ; 8(7)2018 07.
Artículo en Inglés | MEDLINE | ID: mdl-30045883

RESUMEN

Proteins of the FGR1 oncogene partner (or FOP) family are found at microtubule organizing centres (MTOCs) including, in flagellate eukaryotes, the centriole or flagellar basal body from which the axoneme extends. We report conservation of FOP family proteins, TbFOPL and TbOFD1, in the evolutionarily divergent sleeping sickness parasite Trypanosoma brucei, showing (in contrast with mammalian cells, where FOP is essential for flagellum assembly) depletion of a trypanosome FOP homologue, TbFOPL, affects neither axoneme nor flagellum elongation. Instead, TbFOPL depletion causes catastrophic failure in assembly of a lineage-specific, extra-axonemal structure, the paraflagellar rod (PFR). That depletion of centriolar TbFOPL causes failure in PFR assembly is surprising because PFR nucleation commences approximately 2 µm distal from the basal body. When over-expressed with a C-terminal myc-epitope, TbFOPL was also observed at mitotic spindle poles. Little is known about bi-polar spindle assembly during closed trypanosome mitosis, but indication of a possible additional MTOC function for TbFOPL parallels MTOC localization of FOP-like protein TONNEAU1 in acentriolar plants. More generally, our functional analysis of TbFOPL emphasizes significant differences in evolutionary cell biology trajectories of FOP-family proteins. We discuss how at the molecular level FOP homologues may contribute to flagellum assembly and function in diverse flagellates.


Asunto(s)
Proteínas Asociadas a Microtúbulos/genética , Proteínas Asociadas a Microtúbulos/metabolismo , Centro Organizador de los Microtúbulos/metabolismo , Trypanosoma brucei brucei/fisiología , Animales , Axonema/fisiología , Secuencia de Bases , Secuencia Conservada , Evolución Molecular , Flagelos/fisiología , Humanos , Mamíferos/genética , Mamíferos/parasitología , Familia de Multigenes , Proteínas Protozoarias/genética , Proteínas Protozoarias/metabolismo
12.
J Cell Biol ; 217(9): 3255-3266, 2018 09 03.
Artículo en Inglés | MEDLINE | ID: mdl-29945904

RESUMEN

Primary cilia are polarized organelles that allow detection of extracellular signals such as Hedgehog (Hh). How the cytoskeleton supporting the cilium generates and maintains a structure that finely tunes cellular response remains unclear. Here, we find that regulation of actin polymerization controls primary cilia and Hh signaling. Disrupting actin polymerization, or knockdown of N-WASp/Arp3, increases ciliation frequency, axoneme length, and Hh signaling. Cdc42, a potent actin regulator, recruits both atypical protein pinase C iota/lambda (aPKC) and Missing-in-Metastasis (MIM) to the basal body to maintain actin polymerization and restrict axoneme length. Transcriptome analysis implicates the Src pathway as a major aPKC effector. aPKC promotes whereas MIM antagonizes Src activity to maintain proper levels of primary cilia, actin polymerization, and Hh signaling. Hh pathway activation requires Smoothened-, Gli-, and Gli1-specific activation by aPKC. Surprisingly, longer axonemes can amplify Hh signaling, except when aPKC is disrupted, reinforcing the importance of the Cdc42-aPKC-Gli axis in actin-dependent regulation of primary cilia signaling.


Asunto(s)
Actinas/metabolismo , Cilios/metabolismo , Proteínas Hedgehog/metabolismo , Proteína de Unión al GTP cdc42/metabolismo , Células 3T3 , Proteína 3 Relacionada con la Actina/genética , Animales , Axonema/fisiología , Cuerpos Basales/metabolismo , Línea Celular , Activación Enzimática/fisiología , Regulación de la Expresión Génica/fisiología , Ratones , Proteínas de Microfilamentos/metabolismo , Proteínas de Neoplasias/metabolismo , Polimerizacion , Proteína Quinasa C/metabolismo , Transducción de Señal/fisiología , Proteína Neuronal del Síndrome de Wiskott-Aldrich/genética , Proteína con Dedos de Zinc GLI1/metabolismo , Familia-src Quinasas/metabolismo
13.
Reprod Fertil Dev ; 30(6): 833-841, 2018 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-29650062

RESUMEN

Motility analysis of spermatozoa relies on the investigation of either head trajectories or flagellum characteristics. Those two sets of parameters are far from being independent, the flagellum playing the role of motor, whereas the head plays a passive role of cargo. Therefore, quantitative descriptions of head trajectories represent a simplification of the complex pattern of whole sperm cell motion, resulting from the waves developed by the flagellum. The flagellum itself responds to a large variety of signals that precisely control its axoneme to allow activation, acceleration, slowing down or reorientation of the whole spermatozoon. Thus, it is obvious that analysis of flagellum characteristics provides information on the original source of movement and orientation of the sperm cell and presents additional parameters that enrich the panoply of quantitative descriptors of sperm motility. In this review, we briefly describe the methodologies used to obtain good-quality images of fish spermatozoa (head and especially flagellum) while they move fast and the methods developed for their analysis. The paper also aims to establish a link between classical analyses by computer-aided sperm analysis (CASA) and the descriptors generated by fish sperm flagellum analysis, and emphasises the information to be gained regarding motility performance from flagellum motion data.


Asunto(s)
Peces/fisiología , Motilidad Espermática/fisiología , Cola del Espermatozoide/fisiología , Animales , Axonema/fisiología , Procesamiento de Imagen Asistido por Computador , Masculino , Análisis de Semen , Programas Informáticos
14.
Mol Biol Cell ; 29(6): 698-701, 2018 03 15.
Artículo en Inglés | MEDLINE | ID: mdl-29535180

RESUMEN

Motility of cilia (also known as flagella in some eukaryotes) is based on axonemal doublet microtubule sliding that is driven by the dynein molecular motors. Dyneins are organized into intricately patterned inner and outer rows of arms, whose collective activity is to produce inter-microtubule movement. However, to generate a ciliary bend, not all dyneins can be active simultaneously. The switch point model accounts, in part, for how dynein motors are regulated during ciliary movement. On the basis of this model, supported by key direct experimental observations as well as more recent theoretical and structural studies, we are now poised to understand the mechanics of how ciliary dynein coordination controls axonemal bend formation and propagation.


Asunto(s)
Axonema/fisiología , Cilios/fisiología , Dineínas/fisiología , Animales , Chlamydomonas , Cilios/ultraestructura , Microscopía por Crioelectrón , Humanos , Modelos Biológicos , Movimiento
15.
Proc Natl Acad Sci U S A ; 115(5): E1061-E1068, 2018 01 30.
Artículo en Inglés | MEDLINE | ID: mdl-29311312

RESUMEN

Volvox rousseletii is a multicellular spheroidal green alga containing ∼5,000 cells, each equipped with two flagella (cilia). This organism shows striking photobehavior without any known intercellular communication. To help understand how the behavior of flagella is regulated, we developed a method to extract the whole organism with detergent and reactivate its flagellar motility. Upon addition of ATP, demembranated flagella (axonemes) in the spheroids actively beat and the spheroids swam as if they were alive. Under Ca2+-free conditions, the axonemes assumed planar and asymmetrical waveforms and beat toward the posterior pole, as do live spheroids in the absence of light stimulation. In the presence of 10-6 M Ca2+, however, most axonemes beat three-dimensionally toward the anterior pole, similar to flagella in photostimulated live spheroids. This Ca2+-dependent change in flagellar beating direction was more conspicuous near the anterior pole of the spheroid, but was not observed near the posterior pole. This anterior-posterior gradient of flagellar Ca2+ sensitivity may explain the mechanism of V. rousseletii photobehavior.


Asunto(s)
Calcio/fisiología , Detergentes/química , Flagelos/fisiología , Fototaxis , Volvox/fisiología , Adenosina Trifosfato/química , Axonema/fisiología , Microscopía de Contraste de Fase , Movimiento , Fotosíntesis , Grabación en Video
16.
Cell Death Differ ; 25(1): 144-153, 2018 01.
Artículo en Inglés | MEDLINE | ID: mdl-29077094

RESUMEN

The p53 family of transcription factors (p53, p63 and p73) covers a wide range of functions critical for development, homeostasis and health of mammals across their lifespan. Beside the well-established tumor suppressor role, recent evidence has highlighted novel non-oncogenic functions exerted by p73. In particular, p73 is required for multiciliated cell (MCC) differentiation; MCCs have critical roles in brain and airways to move fluids across epithelial surfaces and to transport germ cells in the reproductive tract. This novel function of p73 provides a unifying cellular mechanism for the disparate inflammatory and immunological phenotypes of p73-deficient mice. Indeed, mice with Trp73 deficiency suffer from hydrocephalus, sterility and chronic respiratory tract infections due to profound defects in ciliogenesis and complete loss of mucociliary clearance since MCCs are essential for cleaning airways from inhaled pollutants, pathogens and allergens. Cross-species genomic analyses and functional rescue experiments identify TAp73 as the master transcriptional integrator of ciliogenesis, upstream of previously known central nodes. In addition, TAp73 shows a significant ability to regulate cellular metabolism and energy production through direct transcriptional regulation of several metabolic enzymes, such as glutaminase-2 and glucose-6 phosphate dehydrogenase. This recently uncovered role of TAp73 in the regulation of cellular metabolism strongly affects oxidative balance, thus potentially influencing all the biological aspects associated with p73 function, including development, homeostasis and cancer. Although through different mechanisms, p63 isoforms also contribute to regulation of cellular metabolism, thus indicating a common route used by all family members to control cell fate. At the structural level, the complexity of p73's function is further enhanced by its ability to form heterotetramers with some p63 isoforms, thus indicating the existence of an intrafamily crosstalk that determines the global outcome of p53 family function. In this review, we have tried to summarize all the recent evidence that have emerged on the novel non-oncogenic roles of p73, in an attempt to provide a unified view of the complex function of this gene within its family.


Asunto(s)
Cilios/fisiología , Proteína Tumoral p73/fisiología , Aminoácidos/fisiología , Animales , Axonema/fisiología , Cilios/ultraestructura , Epidermis/crecimiento & desarrollo , Humanos , Metabolismo , Ratones , Estrés Oxidativo , Sistema Respiratorio/ultraestructura , Factores de Transcripción/fisiología , Transcripción Genética , Proteína Tumoral p73/química , Proteína Tumoral p73/genética
17.
Mol Biol Cell ; 29(2): 137-153, 2018 01 15.
Artículo en Inglés | MEDLINE | ID: mdl-29167384

RESUMEN

The nexin-dynein regulatory complex (N-DRC) plays a central role in the regulation of ciliary and flagellar motility. In most species, the N-DRC contains at least 11 subunits, but the specific function of each subunit is unknown. Mutations in three subunits (DRC1, DRC2/CCDC65, DRC4/GAS8) have been linked to defects in ciliary motility in humans and lead to a ciliopathy known as primary ciliary dyskinesia (PCD). Here we characterize the biochemical, structural, and motility phenotypes of two mutations in the DRC2 gene of Chlamydomonas Using high-resolution proteomic and structural approaches, we find that the C-terminal region of DRC2 is critical for the coassembly of DRC2 and DRC1 to form the base plate of N-DRC and its attachment to the outer doublet microtubule. Loss of DRC2 in drc2 mutants disrupts the assembly of several other N-DRC subunits and also destabilizes the assembly of several closely associated structures such as the inner dynein arms, the radial spokes, and the calmodulin- and spoke-associated complex. Our study provides new insights into the range of ciliary defects that can lead to PCD.


Asunto(s)
Proteínas Algáceas/fisiología , Axonema/fisiología , Chlamydomonas/fisiología , Cilios/fisiología , Glicoproteínas/fisiología , Proteínas Algáceas/genética , Chlamydomonas/genética , Glicoproteínas/genética , Mutación , Proteómica
18.
Sci Rep ; 7(1): 17619, 2017 12 15.
Artículo en Inglés | MEDLINE | ID: mdl-29247201

RESUMEN

Male infertility is a major and growing problem and, in most cases, the specific root cause is unknown. Here we show that the transcription factor SOX30 plays a critical role in mouse spermatogenesis. Sox30-null mice are healthy and females are fertile, but males are sterile. In the absence of Sox30 meiosis initiates normally in both sexes but, in males, germ cell development arrests during the post-meiotic round spermatid period. In the mutant testis, acrosome and axoneme development are aberrant, multinucleated germ cells (symplasts) form and round spermatids unable to process beyond step 3 of spermiogenesis. No elongated spermatids nor spermatozoa are produced. Thus, Sox30 represents a rare example of a gene for which loss of function results in a complete arrest of spermatogenesis at the onset of spermiogenesis. Our results suggest that SOX30 mutations may underlie some instances of unexplained non-obstructive azoospermia in humans.


Asunto(s)
Azoospermia/genética , Infertilidad Masculina/genética , Oocitos/crecimiento & desarrollo , Factores de Transcripción SOX/genética , Espermatogénesis/genética , Acrosoma/fisiología , Proteínas Adaptadoras Transductoras de Señales/metabolismo , Animales , Axonema/fisiología , Femenino , Masculino , Meiosis/genética , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Ovario/embriología , Espermátides/citología , Testículo/embriología
19.
Artículo en Inglés | MEDLINE | ID: mdl-28765157

RESUMEN

Ciliary motility is crucial for the development and health of many organisms. Motility depends on the coordinated activity of multiple dynein motors arranged in a precise pattern on the outer doublet microtubules. Although significant progress has been made in elucidating the composition and organization of the dyneins, a comprehensive understanding of dynein regulation is lacking. Here, we focus on two conserved signaling complexes located at the base of the radial spokes. These include the I1/f inner dynein arm associated with radial spoke 1 and the calmodulin- and spoke-associated complex and the nexin-dynein regulatory complex associated with radial spoke 2. Current research is focused on understanding how these two axonemal hubs coordinate and regulate the dynein motors and ciliary motility.


Asunto(s)
Axonema/fisiología , Cilios/fisiología , Dineínas/metabolismo , Animales , Humanos , Movimiento
20.
Theriogenology ; 99: 1-9, 2017 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-28708488

RESUMEN

This study evaluated the semen of two penguin species from separate genera with reference to unique features in sperm structure using light microscopy and transmission electron microscopy. Ejaculates from African penguin (n = 51) and Rockhopper penguin (n = 9) contained on average more than 60% motile spermatozoa and a sperm concentration of 3274 × 106/ml and 1423 × 106/ml, respectively. The percentage progressive motility was similar for the two species as well as all the kinematics parameters. The sperm morphology of these two penguin species is almost identical and largely resembles that of non-passerine birds in terms of the filiform head, small acrosome and mid-piece containing 13 spherical mitochondria, arranged around the proximal and distal centrioles in a single helix. Apart from a shorter mid-piece, penguin sperm morphometrics were similar to other non-passerine birds. The ultrastructure of the sperm principal piece revealed the typical 9 + 2 microtubular arrangement without any outer dense fibres. An unusual feature in both African and Rockhopper penguin spermatozoa was the occurrence of multiple axonemes contained in one plasmalemma in 4% of spermatozoa. These double, triple and quadruple axonemal arrangements have not been described previously albeit multiple tails were reported in other bird species. It is unclear whether such a unique structural feature will be of any advantage for sperm motility and might rather be a result of the absence of sperm competition. Multiple axonemes found in penguin flagella could be an apomorphism that distinguish them from other bird spermatozoa.


Asunto(s)
Axonema/fisiología , Flagelos/fisiología , Motilidad Espermática/fisiología , Espermatozoides/citología , Spheniscidae/fisiología , Animales , Masculino , Especificidad de la Especie
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