RESUMEN
Plants communicate underground by secreting multiple amino acids (AAs) through their roots, triggering defense mechanisms against cadmium (Cd) stress. However, the specific roles of the individual AAs in Cd translocation and detoxification remain unclear. This study investigated how exogenous AAs influence Cd movement from the roots to the shoots in Cd-resistant and Cd-sensitive Chinese cabbage cultivars (Jingcui 60 and 16-7 cultivars). The results showed that methionine (Met) and cysteine (Cys) reduced Cd concentrations in the shoots of Jingcui 60 by approximately 44% and 52%, and in 16-7 by approximately 43% and 32%, respectively, compared to plants treated with Cd alone. However, threonine (Thr) and aspartic acid (Asp) did not show similar effects. Subcellular Cd distribution analysis revealed that AA supplementation increased Cd uptake in the roots, with Jingcui 60 preferentially storing more Cd in the cell wall, whereas the 16-7 cultivar exhibited higher Cd concentrations in the organelles. Moreover, Met and Cys promoted the formation of Cd-phosphate in the roots of Jingcui 60 and Cd-oxalate in the 16-7 cultivar, respectively. Further analysis showed that exogenous Cys inhibited Cd transport to the xylem by downregulating the expression of HMA2 in the roots of both cultivars, and HMA4 in the 16-7 cultivar. These findings provide insights into the influence of exogenous AAs on Cd partitioning and detoxification in Chinese cabbage plants.
Asunto(s)
Aminoácidos , Brassica , Cadmio , Raíces de Plantas , Cadmio/toxicidad , Cadmio/metabolismo , Brassica/metabolismo , Brassica/efectos de los fármacos , Aminoácidos/metabolismo , Raíces de Plantas/metabolismo , Raíces de Plantas/efectos de los fármacos , Transporte Biológico , Brotes de la Planta/metabolismo , Brotes de la Planta/efectos de los fármacos , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Proteínas de Plantas/metabolismo , Proteínas de Plantas/genéticaRESUMEN
Brassica oleracea and Allium vegetables are known for their unique, family specific, water-soluble phytochemicals, glucosinolates, and S-alk(en)yl-l-cysteine sulfoxides, respectively. However, they are also important delivery systems of several other health-related compounds, such as carotenoids (lipid-soluble phytochemicals), vitamin C (water-soluble micronutrient), and vitamin K1 (lipid-soluble micronutrient). When all-year-round availability or transport over long distances is targeted for these often seasonal, locally grown vegetables, processing becomes indispensable. However, the vegetable processing chain, which consists of multiple steps (e.g., pretreatment, preservation, storage, preparation), can impact the nutritional quality of these vegetables corresponding to the nature of the health-related compounds and their susceptibility to (bio)chemical conversions. Since information about the impact of the vegetable processing chain is scattered per compound or processing step, this review targets an integration of the state of the art and discusses needs for future research. Starting with a discussion on substrate-enzyme location within the vegetable matrix, an overview is provided of the impact and potential of processing, encompassing a wide range of (nonenzymatic) conversions.
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Allium , Brassica , Micronutrientes , Fitoquímicos , Verduras , Brassica/química , Brassica/metabolismo , Fitoquímicos/química , Fitoquímicos/metabolismo , Verduras/química , Verduras/metabolismo , Allium/química , Allium/metabolismo , Micronutrientes/análisis , Micronutrientes/metabolismo , Micronutrientes/química , Manipulación de Alimentos , Valor NutritivoRESUMEN
Iodine is an essential trace element in the human diet because it is involved in the synthesis of thyroid hormones. Iodine deficiency affects over 2.2 billion people worldwide, making it a significant challenge to find plant-based sources of iodine that meet the recommended daily intake of this trace element. In this study, cabbage plants were cultivated in a hydroponic system containing iodine at concentrations ranging from 0.01 to 1.0 mg/L in the form of potassium iodide or potassium iodate. During the experiments, plant physiological parameters, biomass production, and concentration changes of iodine and selected microelements in different plant parts were investigated. In addition, the oxidation state of the accumulated iodine in root samples was determined. Results showed that iodine addition had no effect on photosynthetic efficiency and chlorophyll content. Iodide treatment did not considerably stimulate biomass production but iodate treatment increased it at concentrations less than 0.5 mg/L. Increasing iodine concentrations in the nutrient solutions increased iodine content in all plant parts; however, the iodide treatment was 2-7 times more efficient than the iodate treatment. It was concluded, that iodide addition was more favourable on the target element accumulation, however, it should be highlighted that application of this chemical form in nutrient solution decreased the concetrations of selected micoelement concentration comparing with the control plants. It was established that iodate was reduced to iodide during its uptake in cabbage roots, which means that independently from the oxidation number of iodine (+ 5, - 1) applied in the nutrient solutions, the reduced form of target element was transported to the aerial and edible tissues.
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Biofortificación , Brassica , Hidroponía , Yodatos , Yodo , Yodo/metabolismo , Yodo/análisis , Brassica/metabolismo , Brassica/crecimiento & desarrollo , Brassica/efectos de los fármacos , Yodatos/metabolismo , Biomasa , Raíces de Plantas/metabolismo , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/efectos de los fármacos , Fotosíntesis/efectos de los fármacos , Yoduro de Potasio/farmacología , Compuestos de Potasio/farmacología , Compuestos de Potasio/metabolismo , Clorofila/metabolismoRESUMEN
Glucosinolates (GSLs) are plant secondary metabolites commonly found in the cruciferous vegetables of the Brassicaceae family, offering health benefits to humans and defense against pathogens and pests to plants. In this study, we investigated 23 GSL compounds' relative abundance in four tissues of five different Brassica oleracea morphotypes. Using the five corresponding high-quality B. oleracea genome assemblies, we identified 183 GSL-related genes and analyzed their expression with mRNA-Seq data. GSL abundance and composition varied strongly, among both tissues and morphotypes, accompanied by different gene expression patterns. Interestingly, broccoli exhibited a nonfunctional AOP2 gene due to a conserved 2OG-FeII_Oxy domain loss, explaining the unique accumulation of two health-promoting GSLs. Additionally, transposable element (TE) insertions were found to affect the gene structure of MAM3 genes. Our findings deepen the understanding of GSL variation and genetic regulation in B. oleracea morphotypes, providing valuable insights for breeding with tailored GSL profiles in these crops.
Asunto(s)
Brassica , Regulación de la Expresión Génica de las Plantas , Glucosinolatos , Proteínas de Plantas , Transcriptoma , Glucosinolatos/metabolismo , Glucosinolatos/genética , Brassica/genética , Brassica/química , Brassica/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Metabolómica , Productos Agrícolas/genética , Productos Agrícolas/metabolismo , Productos Agrícolas/químicaRESUMEN
BACKGROUND: Kale, a versatile cruciferous crop, valued for its pro-health benefits, stress resistance, and potential applications in forage and cosmetics, holds promise for further enhancement of its bioactive compounds through in vitro cultivation methods. Micropropagation techniques use cytokinins (CKs) which are characterized by various proliferative efficiency. Despite the extensive knowledge regarding CKs, there remains a gap in understanding their role in the physiological mechanisms. That is why, here we investigated the effects of three CKs - kinetin (Kin), 6-benzylaminopurine (BAP), and 2-isopentenyladenine (2iP) - on kale physiology, antioxidant status, steroidal metabolism, and membrane integrity under in vitro cultivation. RESULTS: Our study revealed that while BAP and 2iP stimulated shoot proliferation, they concurrently diminished pigment levels and photosynthetic efficiency. Heightened metabolic activity in response to all CKs was reflected by increased respiratory rate. Despite the differential burst of ROS, the antioxidant properties of kale were associated with the upregulation of guaiacol peroxidase and the scavenging properties of ascorbate rather than glutathione. Notably, CKs fostered the synthesis of sterols, particularly sitosterol, pivotal for cell proliferation and structure of membranes which are strongly disrupted under the action of BAP and 2iP possibly via pathway related to phospholipase D and lipoxygenase which were upregulated. Intriguingly, both CKs treatment spurred the accumulation of sitostenone, known for its ROS scavenging and therapeutic potential. The differential effects of CKs on brassicasterol levels and brassinosteroid (BRs) receptor suggest potential interactions between CKs and BRs. CONCLUSION: Based on the presented results we conclude that the effect evoked by BAP and 2iP in vitro can improve the industrial significance of kale because this treatment makes possible to control proliferation and/or biosynthesis routes of valuable beneficial compounds. Our work offers significant insights into the nuanced effects of CKs on kale physiology and metabolism, illuminating potential avenues for their application in plant biotechnology and medicinal research.
Asunto(s)
Antioxidantes , Citocininas , Cinetina , Reguladores del Crecimiento de las Plantas , Citocininas/metabolismo , Reguladores del Crecimiento de las Plantas/metabolismo , Reguladores del Crecimiento de las Plantas/farmacología , Cinetina/farmacología , Antioxidantes/metabolismo , Brassica/efectos de los fármacos , Brassica/metabolismo , Brassica/fisiología , Brassica/crecimiento & desarrollo , Compuestos de Bencilo/farmacología , Purinas , Fotosíntesis/efectos de los fármacos , Brotes de la Planta/efectos de los fármacos , Brotes de la Planta/metabolismo , Isopenteniladenosina/análogos & derivados , Isopenteniladenosina/metabolismo , Especies Reactivas de Oxígeno/metabolismoRESUMEN
WRKYs play important roles in plant stress resistance. However, the role of WRKYs in non-heading Chinese cabbage (Brassica campestris ssp. chinensis) against Botrytis cinerea (B. cinerea) remains poorly understood. Herein, the expression of BcWRKY1 was induced by B. cinerea. Further, the role of BcWRKY1 in B. cinerea infection was identified. Silencing of BcWRKY1 in non-heading Chinese cabbage enhanced plant resistance to B. cinerea. After B. cinerea inoculation, BcWRKY1-silencing plants exhibited lower reactive oxygen species (ROS) content, higher jasmonic acid (JA) content, and the expression level of JA biosynthesis genes, BcOPR3, BcLOX3-1 and BcLOX3-2 were upregulated. Overexpression of BcWRKY1 in Arabidopsis exhibited a complementary phenotype. By directly targeting W-boxes in the promoter of BcLOX3-2, BcWRKY1 inhibited the transcription of this gene. In addition, 13 candidate interacting proteins of BcWRKY1 were identified by yeast two-hybrid (Y2H) screening, and the interaction between BcWRKY1 and BcCaM6 weakened the inhibition of BcLOX3-2. In summary, our findings suggest that BcWRKY1 interacts with BcCaM6 to negatively regulate disease resistance.
Asunto(s)
Botrytis , Brassica , Ciclopentanos , Resistencia a la Enfermedad , Regulación de la Expresión Génica de las Plantas , Oxilipinas , Enfermedades de las Plantas , Proteínas de Plantas , Botrytis/fisiología , Botrytis/patogenicidad , Ciclopentanos/metabolismo , Enfermedades de las Plantas/microbiología , Enfermedades de las Plantas/genética , Oxilipinas/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Resistencia a la Enfermedad/genética , Brassica/microbiología , Brassica/genética , Brassica/metabolismo , Arabidopsis/microbiología , Arabidopsis/genética , Arabidopsis/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Factores de Transcripción/metabolismo , Factores de Transcripción/genética , Plantas Modificadas GenéticamenteRESUMEN
Despite the precise environmental manipulation enabled by controlled environment agriculture (CEA), plant genotype remains a key factor in producing desirable traits. Brassica rapa var. nipposinica (mizuna) is a leading candidate for supplementing deficiencies in the space diet, however, which cultivar of mizuna will respond best to the environment of the international space station (ISS) is unknown. It is also unclear if there are more inter-varietal (mizuna - mustards) or intra-varietal (mizuna - mizuna) differences in response to the ISS environment. Twenty-two cultivars of mustard greens, including 13 cultivars of mizuna, were grown under ISS-like conditions to determine which would provide the greatest yield and highest concentrations of carotenoids, anthocyanins, calcium, potassium, iron, magnesium, ascorbic acid, thiamine, and phylloquinone. The experiment was conducted thrice, and data were analyzed to determine which cultivar is most suited for further optimization of space-based cultivation. It was found that phylloquinone and ß-carotene concentrations did not vary between cultivars, while all other metrics of interest showed some variation. 'Amara' mustard (B. carinata) provided the best overall nutritional profile, despite its low biomass yield of 36.8 g, producing concentrations of 27.85, 0.40, and 0.65 mg·g - 1 of ascorbic acid, thiamine, and lutein, respectively. Of the mizuna cultivars evaluated, open pollinated mibuna provided the best profile, while 'Red Hybrid' mizuna provided a complimentary profile to that of 'Amara', minimally increasing dietary iron while providing beneficial anthocyanins lacking in 'Amara'.
Asunto(s)
Brassica rapa , Brassica rapa/crecimiento & desarrollo , Brassica rapa/genética , Brassica rapa/metabolismo , Suplementos Dietéticos/análisis , Brassica/crecimiento & desarrollo , Brassica/genética , Brassica/metabolismo , Vuelo EspacialRESUMEN
MAIN CONCLUSION: BcERF98 is induced by ethylene signaling and inhibits the expression of BcFT by interacting with BcNF-YA2 and BcEIP9, thereby inhibiting plant flowering. Several stresses trigger the accumulation of ethylene, which then transmits the signal to ethylene response factors (ERFs) to participate in the regulation of plant development to adapt to the environment. This study clarifies the function of BcERF98, a homolog of AtERF98, in the regulation of plant flowering time mediated by high concentrations of ethylene. Results indicate that BcERF98 is a nuclear and the cell membrane-localized transcription factor and highly responsive to ethylene signaling. BcERF98 inhibits the expression of BcFT by interacting with BcEIP9 and BcNF-YA2, which are related to flowering time regulation, thereby participating in ethylene-mediated plant late flowering regulation. The results have enriched the theoretical knowledge of flowering regulation in non-heading Chinese cabbage (NHCC), providing the scientific basis and gene reserves for cultivating new varieties of NHCC with different flowering times.
Asunto(s)
Etilenos , Flores , Regulación de la Expresión Génica de las Plantas , Proteínas de Plantas , Factores de Transcripción , Flores/genética , Flores/fisiología , Flores/crecimiento & desarrollo , Etilenos/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Factores de Transcripción/metabolismo , Factores de Transcripción/genética , Brassica/genética , Brassica/fisiología , Brassica/metabolismo , Brassica/crecimiento & desarrollo , Transducción de Señal , Reguladores del Crecimiento de las Plantas/metabolismoRESUMEN
The transcription factors Related to ABI3/VP1 (RAV) are crucial for various plant processes and stress responses. Although the U's triangle Brassica species genomes have been released, the knowledge regarding the RAV family is still limited. In this study, we identified 123 putative RAV genes across the six U's triangle Brassica species (Brassica rapa, 14; Brassica oleracea, 14; Brassica nigra, 13; Brassica carinata, 27; Brassica juncea, 28; Brassica napus, 27). Phylogenetic analysis categorized them into three groups. The RAV genes exhibited diversity in both functional and structural aspects, particularly in gene structure and cis-acting elements within their promoters. The expression analysis revealed that BnaRAV genes in Group 1/2 exhibited diverse expression patterns across various tissues, while those in Group 3 did not show expression except for BnaRAV3L-2 and BnaRAV3L-6, which were exclusively expressed in seeds. Furthermore, the seed-specific expression of BnaA06. RAV3L (BnaRAV3L-2) was confirmed through promoter-GUS staining. Subcellular localization studies demonstrated that BnaA06.RAV3L is localized to the nucleus. The overexpression of BnaA06. RAV3L in Arabidopsis led to a remarkable inhibition of seed-specific traits such as seed width, seed length, seed area, and seed weight. This study provides insights into the functional evolution of the RAV gene family in U triangle Brassica species. It establishes a foundation for uncovering the molecular mechanisms underlying the negative role of RAV3L in seed development.
Asunto(s)
Brassica , Regulación de la Expresión Génica de las Plantas , Filogenia , Proteínas de Plantas , Semillas , Factores de Transcripción , Brassica/genética , Brassica/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Factores de Transcripción/genética , Factores de Transcripción/metabolismo , Semillas/genética , Semillas/metabolismo , Semillas/crecimiento & desarrollo , Genoma de Planta , Arabidopsis/genética , Arabidopsis/metabolismoRESUMEN
Physicochemical methods for remediating phenol-contaminated soils are costly and inefficient, making biodegradation an environmentally friendly alternative approach. This study aims to screen for potential phenol-degrading bacteria and to verify the removal capacities of a selected strain in a bioaugmentation experiment at the greenhouse level using Brassica chinensis L. (Chinese cabbage) as the model plant and phenol-contaminated soil. In parallel, pot experiments were conducted using a collaborative approach based on this model system. We found that Myroides xuanwuensis strain H13 showed a high degradation capability, with a 97.67% efficiency in degrading 100 mg/L phenol. Under shaking flask conditions, H13 facilitated the solubilization of tricalcium phosphate and potassium feldspar powder. Pot experiments suggested a phenol removal percentage of 89.22% and enhanced availability of soil phosphorus and potassium for plants with H13 inoculation. In this case, the abundance of soil microbes and the activity of soil enzymes significantly increased as well. Furthermore, both photosynthesis and the antioxidant system in Chinese cabbage were enhanced following H13 inoculation, resulting in its increased yield and quality. Partial least squares path modeling revealed that H13 can primarily affect plant root growth, with a secondary impact on photosynthesis. These findings highlight the potential of biodegradation from phenol-degrading bacteria as a promising strategy for efficient phenol removal from soil while promoting plant growth and health.IMPORTANCEThis study is significant for environmental remediation and agriculture by its exploration of a more environmentally friendly and cost-effective bio-strategy in treating phenol-contaminated soil. These findings have essential implications for environmental remediation efforts and sustainable agriculture. By utilizing the biodegradation capabilities of Myroides xuanwuensis strain H13, it is possible to remove phenol contaminants from the soil efficiently, reducing their negative effects. Furthermore, the enhanced growth and health of the Chinese cabbage plants indicate the potential of this approach to promote sustainable crop production.
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Biodegradación Ambiental , Brassica , Fenol , Microbiología del Suelo , Contaminantes del Suelo , Contaminantes del Suelo/metabolismo , Brassica/crecimiento & desarrollo , Brassica/metabolismo , Brassica/microbiología , Fenol/metabolismo , Suelo/química , Desarrollo de la Planta , Raíces de Plantas/microbiología , Raíces de Plantas/metabolismo , Raíces de Plantas/crecimiento & desarrollo , Bacterias/metabolismo , Bacterias/crecimiento & desarrollo , Bacterias/clasificación , Bacterias/genéticaRESUMEN
Anthocyanin is an important pigment responsible for plant coloration and beneficial to human health. Kale (Brassica oleracea var. acephala), a primary cool-season flowers and vegetables, is an ideal material to study anthocyanin biosynthesis and regulation mechanisms due to its anthocyanin-rich leaves. However, the underlying molecular mechanism of anthocyanin accumulation in kale remains poorly understood. Previously, we demonstrated that BoDFR1 is a key gene controlling anthocyanin biosynthesis in kale. Here, we discovered a 369-bp InDel variation in the BoDFR1 promoter between the two kale inbred lines with different pink coloration, which resulted in reduced transcriptional activity of the BoDFR1 gene in the light-pink line. With the 369-bp insertion as a bait, an R2R3-MYB repressor BoMYB4b was identified using the yeast one-hybrid screening. Knockdown of the BoMYB4b gene led to increased BoDFR1 expression and anthocyanin accumulation. An E3 ubiquitin ligase, BoMIEL1, was found to mediate the degradation of BoMYB4b, thereby promoting anthocyanin biosynthesis. Furthermore, the expression level of BoMYB4b was significantly reduced by light signals, which was attributed to the direct repression of the light-signaling factor BoMYB1R1 on the BoMYB4b promoter. Our study revealed that a novel regulatory module comprising BoMYB1R1, BoMIEL1, BoMYB4b, and BoDFR1 finely regulates anthocyanin accumulation in kale. The findings aim to establish a scientific foundation for genetic improvement of leaf color traits in kale, meanwhile, providing a reference for plant coloration studies.
Asunto(s)
Antocianinas , Brassica , Regulación de la Expresión Génica de las Plantas , Proteínas de Plantas , Antocianinas/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Brassica/genética , Brassica/metabolismo , Regiones Promotoras Genéticas/genética , Ubiquitina-Proteína Ligasas/metabolismo , Ubiquitina-Proteína Ligasas/genética , Hojas de la Planta/metabolismo , Hojas de la Planta/genética , Plantas Modificadas Genéticamente , Factores de Transcripción/metabolismo , Factores de Transcripción/genéticaRESUMEN
Understanding the heavy metals (HMs) tolerance mechanism is crucial for improving plant growth in metal-contaminated soil. In order to evaluate the lead (Pb) tolerance mechanism in Brassica species, a comparative proteomic study was used. Thirteen-day-old seedlings of B. juncea and B. napus were treated with different Pb(NO3)2 concentrations at 0, 3, 30, and 300 mg/L. Under 300 mg/L Pb(NO3)2 concentration, B. napus growth was significantly decreased, while B. juncea maintained normal growth similar to the control. The Pb accumulation was also higher in B. napus root and shoot compared to B. juncea. Gel-free proteomic analysis of roots revealed a total of 68 and 37 differentially abundant proteins (DAPs) in B. juncea and B. napus-specifically, after 300 mg/L Pb exposure. The majority of these proteins are associated with protein degradation, cellular respiration, and enzyme classification. The upregulated RPT2 and tetrapyrrole biosynthesis pathway-associated proteins maintain the cellular homeostasis and photosynthetic rate in B. juncea. Among the 55 common DAPs, S-adenosyl methionine and TCA cycle proteins were upregulated in B. juncea and down-regulated in B. napus after Pb exposure. Furthermore, higher oxidative stress also reduced the antioxidant enzyme activity in B. napus. The current finding suggests that B. juncea is more Pb tolerant than B. napus, possibly due to the upregulation of proteins involved in protein recycling, degradation, and tetrapyrrole biosynthesis pathway.
Asunto(s)
Plomo , Proteínas de Plantas , Proteómica , Tetrapirroles , Plomo/toxicidad , Plomo/metabolismo , Proteínas de Plantas/metabolismo , Proteínas de Plantas/genética , Proteómica/métodos , Tetrapirroles/metabolismo , Tetrapirroles/biosíntesis , Planta de la Mostaza/metabolismo , Planta de la Mostaza/efectos de los fármacos , Planta de la Mostaza/genética , Brassica/metabolismo , Brassica/efectos de los fármacos , Brassica/genética , Raíces de Plantas/metabolismo , Raíces de Plantas/efectos de los fármacosRESUMEN
Tellurium (Te) is a chalcogen element like sulfur and selenium. Although it is unclear whether Te is an essential nutrient in organisms, unique Te metabolic pathways have been uncovered. We have previously reported that an unknown Te metabolite (UKTe) was observed in plants exposed to tellurate, a highly toxic Te oxyanion, by liquid chromatography-inductively coupled plasma mass spectrometer (LC-ICP-MS). In the present study, we detected UKTe in tellurate-exposed broccoli (Brassica oleracea var. italica) by LC-ICP-MS and identified it as gluconic acid-3-tellurate (GA-3Te) using electrospray ionization mass spectrometer with quadrupole-Orbitrap detector and tandem MS analysis, the high-sensitivity and high-resolution mass spectrometry for organic compounds. We also found that GA-3Te was produced from one gluconic acid and one tellurate molecule by direct complexation in an aqueous solution. GA-3Te was significantly less toxic than tellurate on plant growth. This study is the first to identify the Te metabolite GA-3Te in plants and will contribute to the investigation of tellurate detoxification pathways. Moreover, gluconic acid, a natural and biodegradable organic compound, is expected to be applicable to eco-friendly remediation strategies for tellurate contamination.
Asunto(s)
Brassica , Telurio , Brassica/metabolismo , Brassica/química , Telurio/metabolismo , Telurio/química , Espectrometría de Masa por Ionización de Electrospray , Espectrometría de Masas , Espectrometría de Masas en Tándem , Gluconatos/metabolismo , Gluconatos/química , Estructura MolecularRESUMEN
Quantitative transcription regulation studies in vivo and in vitro often make use of reporter proteins. Here we show that using Broccoli aptamers, quantitative study of transcription in various regulatory scenarios is possible without a translational step. To explore the method we studied several regulatory scenarios that we analyzed using thermodynamic occupancy-based models, and found excellent agreement with previous studies. In the next step we show that non-coding DNA can have a dramatic effect on the level of transcription, similar to the influence of the lac repressor with a strong affinity to operator sites. Finally, we point out the limitations of the method in terms of delay times coupled to the folding of the aptamer. We conclude that the Broccoli aptamer is suitable for quantitative transcription measurements.
Asunto(s)
Aptámeros de Nucleótidos , Brassica , Transcripción Genética , Aptámeros de Nucleótidos/metabolismo , Aptámeros de Nucleótidos/química , Brassica/genética , Brassica/metabolismo , Termodinámica , Regulación de la Expresión GénicaRESUMEN
Iodine deficiency in the diet globally continues to be a cause of many diseases and disabilities. Kale is a vegetable that has health-promoting potential because of many nutrients and bioactive compounds (ascorbic acid, carotenoids, glucosinolates and phenolic compounds). Brassica vegetables, including kale, have been strongly recommended as dietary adjuvants for improving health. The nutrient and health-promoting compounds in kale are significantly affected by thermal treatments. Changes in phytochemicals upon such activities may result from two contrary phenomena: breakdown of nutrients and bioactive compounds and a matrix softening effect, which increases the extractability of phytochemicals, which may be especially significant in the case of iodine-fortified kale. This study investigated changes of basic composition, iodine, vitamin C, total carotenoids and polyphenols contents as well as antioxidant activity caused by steaming, blanching and boiling processes in the levels of two cultivars of kale (green and red) non-biofortified and biofortified via the application to nutrient solutions in hydroponic of two iodoquinolines [8-hydroxy-7-iodo-5-quinolinesulfonic acid (8-OH-7-I-5QSA) and 5-chloro-7-iodo-8-quinoline (5-Cl-7-I-8-Q)] and KIO3. Thermal processes generally significantly reduced the content of the components in question and the antioxidant activity of kale, regardless of cultivar and enrichment. It was observed that the red cultivar of kale had a greater ability to accumulate and reduce iodine losses during the culinary processes. 8-hydroxy-7-iodo-5-quinolinesulfonic acid showed a protective effect against the treatments used, compared to other enrichments, thus contributing to the preservation of high iodine content.
Asunto(s)
Antioxidantes , Brassica , Calor , Yodo , Brassica/química , Brassica/metabolismo , Yodo/análisis , Antioxidantes/análisis , Antioxidantes/metabolismo , Carotenoides/análisis , Carotenoides/metabolismo , Ácido Ascórbico/análisis , Ácido Ascórbico/metabolismo , Polifenoles/análisis , Alimentos Fortificados/análisisRESUMEN
Metabolic engineering enables oilseed crops to be more competitive by having more attractive properties for oleochemical industrial applications. The aim of this study was to increase the erucic acid level and to produce wax ester (WE) in seed oil by genetic transformation to enhance the industrial applications of B. carinata. Six transgenic lines for high erucic acid and fifteen transgenic lines for wax esters were obtained. The integration of the target genes for high erucic acid (BnFAE1 and LdPLAAT) and for WEs (ScWS and ScFAR) in the genome of B. carinata cv. 'Derash' was confirmed by PCR analysis. The qRT-PCR results showed overexpression of BnFAE1 and LdPLAAT and downregulation of RNAi-BcFAD2 in the seeds of the transgenic lines. The fatty acid profile and WE content and profile in the seed oil of the transgenic lines and wild type grown in biotron were analyzed using gas chromatography and nanoelectrospray coupled with tandem mass spectrometry. A significant increase in erucic acid was observed in some transgenic lines ranging from 19% to 29% in relation to the wild type, with a level of erucic acid reaching up to 52.7%. Likewise, the transgenic lines harboring ScFAR and ScWS genes produced up to 25% WE content, and the most abundant WE species were 22:1/20:1 and 22:1/22:1. This study demonstrated that metabolic engineering is an effective biotechnological approach for developing B. carinata into an industrial crop.
Asunto(s)
Brassica , Ácidos Erucicos , Ésteres , Ingeniería Metabólica , Plantas Modificadas Genéticamente , Semillas , Ceras , Ácidos Erucicos/metabolismo , Ingeniería Metabólica/métodos , Plantas Modificadas Genéticamente/genética , Plantas Modificadas Genéticamente/metabolismo , Ceras/metabolismo , Ésteres/metabolismo , Semillas/genética , Semillas/metabolismo , Brassica/genética , Brassica/metabolismo , Ácidos Grasos/metabolismo , Aceites de Plantas/metabolismo , Regulación de la Expresión Génica de las Plantas , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismoRESUMEN
The indiscriminate use of zinc oxide nanoparticles (ZnO NPs) in daily life can lead to their release into soil environment. These ZnO NPs can be taken up by crops and translocated to their edible part, potentially causing risks to the ecosystem and human health. In this study, we conducted pot experiments to determine phytotoxicity, bioaccumulation and translocation depending on the size (10 - 30â¯nm, 80 - 200â¯nm and 300â¯nm diameter) and concentration (0, 100, 500 and 1000â¯mg Zn/kg) of ZnO NPs and Zn ion (Zn2+) in bok choy, a leafy green vegetable crop. After 14 days of exposure, our results showed that large-sized ZnO NPs (i.e., 300â¯nm) at the highest concentration exhibited greater phytotoxicity, including obstruction of leaf and root weight (42.5â¯% and 33.8â¯%, respectively) and reduction of chlorophyll a and b content (50.2â¯% and 85.2â¯%, respectively), as well as changes in the activities of oxidative stress responses compared to those of small-sized ZnO NPs, although their translocation ability was relatively lower than that of smaller ones. The translocation factor (TF) values decreased as the size of ZnO NPs increased, with TF values of 0.68 for 10 - 30â¯nm, 0.55 for 80 - 200â¯nm, and 0.27 for 300â¯nm ZnO NPs, all at the highest exposure concentration. Both the results of micro X-ray fluorescence (µ-XRF) spectrometer and bio-transmission electron microscopy (bio-TEM) showed that the Zn elements were mainly localized at the edges of leaves exposed to small-sized ZnO NPs. However, the Zn elements upon exposure to large-sized ZnO NP were primarily observed in the primary veins of leaves in the µ-XRF data, indicating a limitation in their ability to translocate from roots to leaves. This study not only advances our comprehension of the environmental impact of nanotechnology but also holds considerable implications for the future of sustainable agriculture and food safety.
Asunto(s)
Bioacumulación , Brassica , Nanopartículas del Metal , Tamaño de la Partícula , Hojas de la Planta , Contaminantes del Suelo , Óxido de Zinc , Óxido de Zinc/toxicidad , Óxido de Zinc/química , Contaminantes del Suelo/toxicidad , Brassica/efectos de los fármacos , Brassica/metabolismo , Brassica/crecimiento & desarrollo , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/metabolismo , Nanopartículas del Metal/toxicidad , Suelo/química , Clorofila/metabolismo , Estrés Oxidativo/efectos de los fármacos , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/metabolismo , Clorofila A/metabolismo , Nanopartículas/toxicidadRESUMEN
Arid regions can benefit from using native desert plants, which require minimal freshwater and can aid in remediating soil phytotoxic metals (PTMs) from traffic emissions. In this study, we assessed the ability of three native desert plants-Pennisetum divisum, Tetraena qatarensis, and Brassica tournefortii-to accumulate phytotoxic metals (PTMs) in their different plant organs, including leaves, stems, and roots/rhizomes. The PTMs were analyzed in soil and plant samples collected from Dubai, United Arab Emirates (UAE). The results indicated significantly higher levels of PTMs on the soil surface than the subsurface layer. Brassica exhibited the highest concentrations of Fe and Zn, measuring 566.7 and 262.8 mg kg-1, respectively, while Tetraena accumulated the highest concentration of Sr (1676.9 mg kg-1) in their stems. In contrast, Pennisetum recorded the lowest concentration of Sr (21.0 mg kg-1), while Tetraena exhibited the lowest concentrations of Fe and Zn (22.5 and 30.1 mg kg-1) in their leaves. The roots of Pennisetum, Brassica, and Tetraena demonstrated the potential to accumulate Zn from the soil, with concentration factors (CF) of 1.75, 1.09, and 1.09, respectively. Moreover, Brassica exhibited the highest CF for Sr, measuring 2.34. Pennisetum, however, could not translocate PTMs from its rhizomes to other plant organs, as indicated by a translocation factor (TF) of 1. In contrast, Brassica effectively translocated the studied PTMs from its roots to the stem and leaves (except for Sr in the leaves). Furthermore, Pennisetum exclusively absorbed Zn from the soil into its leaves and stems, with an enrichment factor (EF) greater than 1. Brassica showed the ability to uptake the studied PTMs in its stem and leaves (except for Fe), while Tetraena primarily absorbed Sr and Zn into its stems. Based on the CF and TF results, Pennisetum appears to be a suitable species for phytostabilization of both Fe and Zn, while Brassica is well-suited for Sr and Zn polluted soils. Tetraena shows potential for Zn phytoremediation. These findings suggest that these plants are suitable for PTMs phytoextraction. Furthermore, based on the EF results, these plants can efficiently sequester PTMs.
Asunto(s)
Biodegradación Ambiental , Ciudades , Contaminantes del Suelo , Contaminantes del Suelo/metabolismo , Contaminantes del Suelo/análisis , Pennisetum/metabolismo , Clima Desértico , Suelo/química , Raíces de Plantas/metabolismo , Hojas de la Planta/metabolismo , Brassica/metabolismo , Brassica/crecimiento & desarrollo , Metales Pesados/metabolismo , Metales Pesados/análisisRESUMEN
Traditional heavy metal pollution, such as cadmium, impacts the transformation and risks of bisphenol pollutants (like bisphenol A, BPA), in plants, especially due to the ubiquitous presence of bromide ion. Although it has been discovered that the bromination of phenolic pollutants occurs in plants, thereby increasing the associated risks, the influence and mechanisms of bromination under complex contamination conditions involving both heavy metals and phenolic compounds remain poorly understood. This study addresses the issue by exposing Brassica chinensis L. to cadmium ion (Cd2+, 25-100 µM), with the hydroponic solution containing BPA (15 mg/L) and bromide ion (0.5 mM) in this work. It was observed that Cd2+ primarily enhanced the bromination of BPA by elevating the levels of reactive oxygen species (ROS) and the activity of peroxidase (POD) in Brassica chinensis L. The variety of bromination products within Brassica chinensis L. increased as the concentration of Cd2+ rose from 25 to 100 µM. The substitution positions of bromine were determined using Gaussian calculations and mass spectrometry analysis. The toxicity of bromination products derived from BPA was observed to increase based on Ecological Structure-Activity Relationships analysis and HepG2 cytotoxicity assays. This study provides new insights into the risks and health hazards associated with cadmium pollution, particularly its role in enhancing the bromination of bisphenol pollutants in plants.
Asunto(s)
Compuestos de Bencidrilo , Brassica , Cadmio , Halogenación , Fenoles , Brassica/metabolismo , Brassica/efectos de los fármacos , Compuestos de Bencidrilo/metabolismo , Compuestos de Bencidrilo/toxicidad , Fenoles/metabolismo , Fenoles/toxicidad , Cadmio/metabolismo , Cadmio/toxicidadRESUMEN
Kimchi cabbage, the key ingredient in kimchi, is cultivated year-round to meet high production demands. This study aimed to examine the effects of seasonal harvesting (spring, summer, fall, and winter) on the microbial and metabolic profiles of kimchi during 30 days of fermentation. Lactic acid bacteria distribution is notably influenced by seasonal variations, with Latilactobacillus dominant in fall-harvested kimchi group and Weissella prevailing in spring, summer, and winter. The microbial communities of spring and fall group exhibited similar profiles before fermentation, whereas the microbial communities and metabolic profiles of spring and summer group were similar after 30 days of fermentation. Seasonal disparities in metabolite concentrations, including glutamic acid, serine, and cytosine, persist throughout fermentation. This study provides a comprehensive understanding of the substantial impact of seasonal harvesting of kimchi cabbage on the microbial and metabolic characteristics of kimchi, providing valuable insights into producing kimchi with diverse qualities.