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1.
Elife ; 102021 09 22.
Artículo en Inglés | MEDLINE | ID: mdl-34550876

RESUMEN

Eukaryotes generally display a circadian rhythm as an adaption to the reoccurring day/night cycle. This is particularly true for visual physiology that is directly affected by changing light conditions. Here we investigate the influence of the circadian rhythm on the expression and function of visual transduction cascade regulators in diurnal zebrafish and nocturnal mice. We focused on regulators of shut-off kinetics such as Recoverins, Arrestins, Opsin kinases, and Regulator of G-protein signaling that have direct effects on temporal vision. Transcript as well as protein levels of most analyzed genes show a robust circadian rhythm-dependent regulation, which correlates with changes in photoresponse kinetics. Electroretinography demonstrates that photoresponse recovery in zebrafish is delayed in the evening and accelerated in the morning. Functional rhythmicity persists in continuous darkness, and it is reversed by an inverted light cycle and disrupted by constant light. This is in line with our finding that orthologous gene transcripts from diurnal zebrafish and nocturnal mice are often expressed in an anti-phasic daily rhythm.


Asunto(s)
Ritmo Circadiano/efectos de la radiación , Células Fotorreceptoras de Vertebrados/efectos de la radiación , Células Fotorreceptoras Retinianas Conos/efectos de la radiación , Animales , Arrestinas/genética , Arrestinas/metabolismo , Oscuridad , Electrorretinografía , Femenino , Quinasa 1 del Receptor Acoplado a Proteína-G/genética , Quinasa 1 del Receptor Acoplado a Proteína-G/metabolismo , Luz , Fototransducción , Masculino , Ratones , Modelos Animales , Células Fotorreceptoras de Vertebrados/metabolismo , Proteínas RGS/genética , Proteínas RGS/metabolismo , Células Fotorreceptoras Retinianas Conos/metabolismo , Visión Ocular/efectos de la radiación , Pez Cebra/genética , Pez Cebra/metabolismo
2.
Biomed Res Int ; 2021: 3236892, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34055970

RESUMEN

BACKGROUND: To investigate the chronic photodamage induced by the low-intensity blue light of phones, we carried out a clinical pilot study and established an animal model by irradiating SD rats with a homemade illuminator. METHODS: Clinical investigation: A total of 25 clinical medical workers in our hospital were selected and divided into a control group and an observation group according to the daily video terminal use time. Multifocal electrophysiological system (Mf-ERG) was used for retinal functional examination. Animal experiment: A total of sixty SD rats were randomly divided into a control group (n = 6) and an experimental group (n = 54). The experimental rats were divided into nine groups, which were exposed to the blue light illuminator of the simulated cell phone array for different time. The visual electrophysiology of the rats was tested, and changes in structure were observed by H&E staining and transmission electron microscopy. RESULTS: In clinical investigation, macular centers near the concave area retinal photoreceptor cells have reduced amplitude. In animal experiments, the amplitude of photoreceptor cells decreased, the peak time was delayed, and the amplitudes were lower in the experimental groups. H&E staining and transmission electron microscope showed retinal tissue structure and functional damage in experimental groups. CONCLUSIONS: Long-term exposure to low-illuminance blue light can cause retinal tissue structure and functional damage, and the chronic damage due to low-illuminance light warrants attention. The clinical registration number is 2018-KY-KS-LHL.


Asunto(s)
Teléfono Celular , Lesiones Oculares/etiología , Luz/efectos adversos , Retinaldehído/efectos de la radiación , Adulto , Animales , Modelos Animales de Enfermedad , Lesiones Oculares/patología , Femenino , Humanos , Masculino , Estimulación Luminosa , Células Fotorreceptoras de Vertebrados/efectos de la radiación , Ratas , Retina/patología , Retina/efectos de la radiación , Adulto Joven
3.
Mol Vis ; 27: 206-220, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33967574

RESUMEN

Purpose: To explore synaptic changes and the response of microglia in a light-induced photoreceptor degeneration model. Methods: Sprague-Dawley rats were euthanized 1 h, 1 day, 3 days, 7 days, and 14 days after being exposed to intense blue light for 24 h. Hematoxylin and eosin (H&E) and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining were used to evaluate changes in the outer nuclear layer (ONL). Transmission electron microscopy (TEM) was applied to observe the ultrastructural changes in the synapses between the photoreceptors and second-order neurons. Western blotting was conducted to evaluate specific proteins, including postsynaptic density-95 (PSD-95), metabotropic glutamate receptor 6 (mGluR6), synapsin I, and synaptophysin. Immunofluorescence of CD11b and PKC-α or mGluR6 was used to explore the spatial relationships between microglial processes and synaptic elements. Immunoelectron microscopy of PSD-95 was performed to further confirm its engulfment of synaptic materials. Results: H&E and TUNEL staining showed that the thickness of the ONL decreased markedly, and the number of apoptotic photoreceptors peaked at day 1. TEM revealed darkened photoreceptor terminals and that ribbons of them were floating in the cytoplasm, coinciding with the downregulation of PSD-95 and mGluR6. Downstream synaptic protein synapsin I and synaptophysin exhibited upregulation in the inner plexiform layer. Activated microglia migrated to the outer retina, and their processes were found in close proximity to synapses in the outer plexiform layer under light and electron microscopy levels. Double immunostaining of CD11b and mGluR6 showed colocalization. PSD-95-immunoreactive electron-dense materials were observed inside the microglia suggesting engulfment of synaptic components. Conclusions: The study showed that there are early synaptic impairment and late compensatory changes in downstream synapses in this photic injury model. Activated microglia touched and directly engulfed synaptic materials. Microglia may play a role or a partial role in synaptic changes.


Asunto(s)
Luz/efectos adversos , Microglía/fisiología , Células Fotorreceptoras de Vertebrados/efectos de la radiación , Traumatismos Experimentales por Radiación/fisiopatología , Degeneración Retiniana/fisiopatología , Sinapsis/fisiología , Animales , Western Blotting , Modelos Animales de Enfermedad , Homólogo 4 de la Proteína Discs Large/metabolismo , Etiquetado Corte-Fin in Situ , Masculino , Microscopía Electrónica de Transmisión , Microscopía Inmunoelectrónica , Células Fotorreceptoras de Vertebrados/metabolismo , Células Fotorreceptoras de Vertebrados/ultraestructura , Traumatismos Experimentales por Radiación/etiología , Traumatismos Experimentales por Radiación/metabolismo , Ratas , Ratas Sprague-Dawley , Receptores de Glutamato Metabotrópico/metabolismo , Degeneración Retiniana/etiología , Degeneración Retiniana/metabolismo , Sinapsinas/metabolismo , Sinaptofisina/metabolismo
4.
Opt Express ; 29(2): 552-563, 2021 Jan 18.
Artículo en Inglés | MEDLINE | ID: mdl-33726288

RESUMEN

An optical platform is presented for examining intrinsic contrast detection strategies when imaging retinal structure using ex vivo tissue. A custom microscope was developed that scans intact tissue and collects scattered light distribution at every image pixel, allowing digital masks to be applied after image collection. With this novel approach at measuring the spatial distribution of multiply scattered light, known and novel methods of detecting intrinsic cellular contrast can be explored, compared, and optimized for retinal structures of interest.


Asunto(s)
Sensibilidad de Contraste/fisiología , Microscopía/instrumentación , Células Fotorreceptoras de Vertebrados/efectos de la radiación , Dispersión de Radiación , Animales , Diseño de Equipo , Luz , Sciuridae
5.
Int J Med Sci ; 18(1): 109-119, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33390779

RESUMEN

Near infrared (NIR) light therapy, or photobiomodulation therapy (PBMT), has gained persistent worldwide attention in recent years as a new novel scientific approach for therapeutic applications in ophthalmology. This ongoing therapeutic adoption of NIR therapy is largely propelled by significant advances in the fields of photobiology and bioenergetics, such as the discovery of photoneuromodulation by cytochrome c oxidase and the elucidation of therapeutic biochemical processes. Upon transcranial delivery, NIR light has been shown to significantly increase cytochrome oxidase and superoxide dismutase activities which suggests its role in inducing metabolic and antioxidant beneficial effects. Furthermore, NIR light may also boost cerebral blood flow and cognitive functions in humans without adverse effects. In this review, we highlight the value of NIR therapy as a novel paradigm for treatment of visual and neurological conditions, and provide scientific evidence to support the use of NIR therapy with emphasis on molecular and cellular mechanisms in eye diseases.


Asunto(s)
Oftalmopatías/terapia , Terapia por Luz de Baja Intensidad/métodos , Apoptosis/efectos de la radiación , Complejo IV de Transporte de Electrones/metabolismo , Oftalmopatías/patología , Humanos , Terapia por Luz de Baja Intensidad/instrumentación , Mitocondrias/metabolismo , Mitocondrias/patología , Mitocondrias/efectos de la radiación , Células Fotorreceptoras de Vertebrados/citología , Células Fotorreceptoras de Vertebrados/metabolismo , Células Fotorreceptoras de Vertebrados/efectos de la radiación , Superóxido Dismutasa/metabolismo , Resultado del Tratamiento
6.
Aging (Albany NY) ; 12(16): 16579-16596, 2020 08 28.
Artículo en Inglés | MEDLINE | ID: mdl-32858529

RESUMEN

Excessive light exposure is a principal environmental factor, which can cause damage to photoreceptors and retinal pigment epithelium (RPE) cells and may accelerate the progression of age-related macular degeneration (AMD). In this study, oxidative stress, endoplasmic reticulum (ER) stress and autophagy caused by light exposure were evaluated in vitro and in vivo. Light exposure caused severe photo-oxidative stress and ER stress in photoreceptors (661W cells) and RPE cells (ARPE-19 cells). Suppressing either oxidative stress or ER stress was protective against light damage in 661W and ARPE-19 cells and N-acetyl-L-cysteine treatment markedly inhibited the activation of ER stress caused by light exposure. Moreover, suppressing autophagy with 3-methyladenine significantly attenuated light-induced cell death. Additionally, inhibiting ER stress either by knocking down PERK signals or with GSK2606414 treatment remarkably suppressed prolonged autophagy and protected the cells against light injury. In vivo experiments verified neuroprotection via inhibiting ER stress-related autophagy in light-damaged retinas of mice. In conclusion, the above results suggest that light-induced photo-oxidative stress may trigger subsequent activation of ER stress and prolonged autophagy in photoreceptors and RPE cells. Suppressing ER stress may abrogate over-activated autophagy and protect the retina against light injury.


Asunto(s)
Autofagia/efectos de los fármacos , Estrés del Retículo Endoplásmico/efectos de los fármacos , Luz/efectos adversos , Fármacos Neuroprotectores/farmacología , Estrés Oxidativo/efectos de los fármacos , Células Fotorreceptoras de Vertebrados/efectos de los fármacos , Epitelio Pigmentado de la Retina/efectos de los fármacos , Acetilcisteína/farmacología , Adenina/análogos & derivados , Adenina/farmacología , Animales , Antioxidantes/farmacología , Autofagia/efectos de la radiación , Línea Celular , Estrés del Retículo Endoplásmico/efectos de la radiación , Humanos , Indoles/farmacología , Masculino , Ratones Endogámicos C57BL , Estrés Oxidativo/efectos de la radiación , Células Fotorreceptoras de Vertebrados/metabolismo , Células Fotorreceptoras de Vertebrados/patología , Células Fotorreceptoras de Vertebrados/efectos de la radiación , Epitelio Pigmentado de la Retina/metabolismo , Epitelio Pigmentado de la Retina/patología , Epitelio Pigmentado de la Retina/efectos de la radiación , eIF-2 Quinasa/genética , eIF-2 Quinasa/metabolismo
7.
Invest Ophthalmol Vis Sci ; 61(10): 47, 2020 08 03.
Artículo en Inglés | MEDLINE | ID: mdl-32852543

RESUMEN

Purpose: Maintaining levels of nicotinamide adenine dinucleotide (NAD+), a coenzyme critical for cellular energetics and biosynthetic pathways, may be therapeutic in retinal disease because retinal NAD+ levels decline during retinal damage and degeneration. The purpose of this study was to investigate whether systemic treatment with nicotinamide riboside (NR), a NAD+ precursor that is orally deliverable and well-tolerated by humans, is protective in a mouse model of light-induced retinal degeneration. Methods: Mice were injected intraperitoneally with vehicle or NR the day before and the morning of exposure to degeneration-inducing levels of light. Retinal function was assessed by electroretinography and in vivo retinal morphology and inflammation was assessed by optical coherence tomography. Post mortem retina sections were assessed for morphology, TUNEL, and inflammatory markers Iba1 and GFAP. Retinal NAD+ levels were enzymatically assayed. Results: Exposure to degeneration-inducing levels of light suppressed retinal NAD+ levels. Mice undergoing light-induced retinal degeneration exhibited significantly suppressed retinal function, severely disrupted photoreceptor cell layers, and increased apoptosis and inflammation in the outer retina. Treatment with NR increased levels of NAD+ in retina and prevented these deleterious outcomes. Conclusions: This study is the first to report the protective effects of NR treatment in a mouse model of retinal degeneration. The positive outcomes, coupled with human tolerance to NR dosing, suggest that maintaining retinal NAD+ via systemic NR treatment should be further explored for clinical relevance.


Asunto(s)
Niacinamida/análogos & derivados , Degeneración Retiniana/prevención & control , Animales , Modelos Animales de Enfermedad , Electrorretinografía , Técnica del Anticuerpo Fluorescente , Inyecciones Intraperitoneales , Luz/efectos adversos , Masculino , Ratones , Ratones Endogámicos BALB C , NAD/metabolismo , Niacinamida/administración & dosificación , Niacinamida/uso terapéutico , Células Fotorreceptoras de Vertebrados/efectos de los fármacos , Células Fotorreceptoras de Vertebrados/metabolismo , Células Fotorreceptoras de Vertebrados/efectos de la radiación , Compuestos de Piridinio , Retina/diagnóstico por imagen , Retina/efectos de los fármacos , Retina/metabolismo , Retina/efectos de la radiación , Degeneración Retiniana/diagnóstico por imagen , Degeneración Retiniana/etiología , Tomografía de Coherencia Óptica
8.
Mar Drugs ; 18(8)2020 Jul 25.
Artículo en Inglés | MEDLINE | ID: mdl-32722441

RESUMEN

Light-emitting diodes (LEDs) are widely used and energy-efficient light sources in modern life that emit higher levels of short-wavelength blue light. Excessive blue light exposure may damage the photoreceptor cells in our eyes. Astaxanthin, a xanthophyll that is abundantly available in seafood, is a potent free radical scavenger and anti-inflammatory agent. We used a 661W photoreceptor cell line to investigate the protective effect of astaxanthin on blue light LED-induced retinal injury. The cells were treated with various concentrations of astaxanthin and then exposed to blue light LED. Our results showed that pretreatment with astaxanthin inhibited blue light LED-induced cell apoptosis and prevented cell death. Moreover, the protective effect was concentration dependent. Astaxanthin suppressed the production of reactive oxygen species and oxidative stress biomarkers and diminished mitochondrial damage induced by blue light exposure. Western blot analysis confirmed that astaxanthin activated the PI3K/Akt pathway, induced the nuclear translocation of Nrf2, and increased the expression of phase II antioxidant enzymes. The expression of antioxidant enzymes and the suppression of apoptosis-related proteins eventually protected the 661W cells against blue light LED-induced cell damage. Thus, our results demonstrated that astaxanthin exerted a dose-dependent protective effect on photoreceptor cells against damage mediated by blue light LED exposure.


Asunto(s)
Depuradores de Radicales Libres/farmacología , Factor 2 Relacionado con NF-E2/metabolismo , Estrés Oxidativo/efectos de los fármacos , Fosfatidilinositol 3-Quinasa/metabolismo , Células Fotorreceptoras de Vertebrados/efectos de los fármacos , Proteínas Proto-Oncogénicas c-akt/metabolismo , Protectores contra Radiación/farmacología , Animales , Apoptosis/efectos de los fármacos , Proteínas Reguladoras de la Apoptosis/metabolismo , Línea Celular , Color , Luz , Ratones , Mitocondrias/efectos de los fármacos , Mitocondrias/enzimología , Mitocondrias/patología , Mitocondrias/efectos de la radiación , Células Fotorreceptoras de Vertebrados/enzimología , Células Fotorreceptoras de Vertebrados/patología , Células Fotorreceptoras de Vertebrados/efectos de la radiación , Transducción de Señal , Xantófilas/farmacología
9.
J Gerontol A Biol Sci Med Sci ; 75(9): e49-e52, 2020 09 16.
Artículo en Inglés | MEDLINE | ID: mdl-32596723

RESUMEN

The age spectrum of human populations is shifting toward the older with larger proportions suffering physical decline. Mitochondria influence the pace of aging as the energy they provide for cellular function in the form of adenosine triphosphate (ATP) declines with age. Mitochondrial density is greatest in photoreceptors, particularly cones that have high energy demands and mediate color vision. Hence, the retina ages faster than other organs, with a 70% ATP reduction over life and a significant decline in photoreceptor function. Mitochondria have specific light absorbance characteristics influencing their performance. Longer wavelengths spanning 650->1,000 nm improve mitochondrial complex activity, membrane potential, and ATP production. Here, we use 670-nm light to improve photoreceptor performance and measure this psychophysically in those aged 28-72 years. Rod and cone performance declined significantly after approximately 40 years of age. 670-nm light had no impact in younger individuals, but in those around 40 years and older, significant improvements were obtained in color contrast sensitivity for the blue visual axis (tritan) known to display mitochondrial vulnerability. The red visual axis (protan) improved but not significantly. Rod thresholds also improved significantly in those >40 years. Using specific wavelengths to enhance mitochondrial performance will be significant in moderating the aging process in this metabolically demanding tissue.


Asunto(s)
Envejecimiento/fisiología , Mitocondrias/fisiología , Trastornos de la Visión/etiología , Adulto , Anciano , Envejecimiento/efectos de la radiación , Femenino , Humanos , Masculino , Persona de Mediana Edad , Mitocondrias/efectos de la radiación , Células Fotorreceptoras de Vertebrados/fisiología , Células Fotorreceptoras de Vertebrados/efectos de la radiación
10.
Sci Rep ; 10(1): 6376, 2020 04 14.
Artículo en Inglés | MEDLINE | ID: mdl-32286488

RESUMEN

The light damage (LD) model was mainly used to study some of the main aspects of age related macular degeneration (AMD), such as oxidative stress and photoreceptor death. Several protocols of light-induced retinal degeneration exist. Acute light damage is characterized by a brief exposure (24 hours) to high intensity light (1000 lux) and leads to focal degeneration of the retina which progresses over time. To date there are not experimental data that relate this model to neovascular events. Therefore, the purpose of this study was to characterize the retina after an acute light damage to assess whether the vascularization was affected. Functional, molecular and morphological investigations were carried out. The electroretinographic response was assessed at all recovery times (7, 60, 120 days after LD). Starting from 7 days after light damage there was a significant decrease in the functional response, which remained low up to 120 days of recovery. At 7 days after light exposure, neo-vessels invaded the photoreceptor layer and retinal neovascularization occurred. Remarkably, neoangiogenesis was associated to the up-regulation of VEGF, bFGF and their respective receptors (VEGFR2 and FGFR1) with the progression of degeneration. These important results indicate that a brief exposure to bright light induces the up-regulation of pro-angiogenic pathways with subsequent neovascularization.


Asunto(s)
Degeneración Macular/metabolismo , Neovascularización Patológica/metabolismo , Traumatismos Experimentales por Radiación/metabolismo , Retina , Animales , Células Fotorreceptoras de Vertebrados/patología , Células Fotorreceptoras de Vertebrados/efectos de la radiación , Ratas Sprague-Dawley , Retina/lesiones , Retina/metabolismo , Regulación hacia Arriba , Factor A de Crecimiento Endotelial Vascular/metabolismo
11.
Int J Mol Sci ; 21(7)2020 Mar 30.
Artículo en Inglés | MEDLINE | ID: mdl-32235464

RESUMEN

Potent neuroprotective effects of photobiomodulation with 670 nm red light (RL) have been demonstrated in several models of retinal disease. RL improves mitochondrial metabolism, reduces retinal inflammation and oxidative cell stress, showing its ability to enhance visual function. However, the current knowledge is limited to the main hypothesis that the respiratory chain complex IV, cytochrome c oxidase, serves as the primary target of RL. Here, we demonstrate a comprehensive cellular, molecular, and functional characterization of neuroprotective effects of 670 nm RL and 810 nm near-infrared light (NIRL) on blue light damaged murine primary photoreceptors. We show that respiratory chain complexes I and II are additional PBM targets, besides complex IV, leading to enhanced mitochondrial energy metabolism. Accordingly, our study identified mitochondria related RL- and NIRL-triggered defense mechanisms promoting photoreceptor neuroprotection. The observed improvement of mitochondrial and extramitochondrial respiration in both inner and outer segments is linked with reduced oxidative stress including its cellular consequences and reduced mitochondria-induced apoptosis. Analysis of regulatory mechanisms using gene expression analysis identified upregulation α-crystallins that indicate enhanced production of proteins with protective functions that point to the rescued mitochondrial function. The results support the hypothesis that energy metabolism is a major target for retinal light therapy.


Asunto(s)
Terapia por Luz de Baja Intensidad , Neuroprotección/efectos de la radiación , Células Fotorreceptoras de Vertebrados/efectos de la radiación , Degeneración Retiniana/terapia , Animales , Femenino , Rayos Infrarrojos/uso terapéutico , Terapia por Luz de Baja Intensidad/métodos , Masculino , Ratones Endogámicos C57BL , Células Fotorreceptoras de Vertebrados/metabolismo , Células Fotorreceptoras de Vertebrados/patología , Degeneración Retiniana/genética , Degeneración Retiniana/patología , Regulación hacia Arriba/efectos de la radiación , alfa-Cristalinas/genética
12.
Elife ; 92020 04 03.
Artículo en Inglés | MEDLINE | ID: mdl-32242818

RESUMEN

Photoreceptor degeneration is a major cause of blindness and a considerable health burden during aging but effective therapeutic or preventive strategies have not so far become readily available. Here, we show in mouse models that signaling through the tyrosine kinase receptor KIT protects photoreceptor cells against both light-induced and inherited retinal degeneration. Upon light damage, photoreceptor cells upregulate Kit ligand (KITL) and activate KIT signaling, which in turn induces nuclear accumulation of the transcription factor NRF2 and stimulates the expression of the antioxidant gene Hmox1. Conversely, a viable Kit mutation promotes light-induced photoreceptor damage, which is reversed by experimental expression of Hmox1. Furthermore, overexpression of KITL from a viral AAV8 vector prevents photoreceptor cell death and partially restores retinal function after light damage or in genetic models of human retinitis pigmentosa. Hence, application of KITL may provide a novel therapeutic avenue for prevention or treatment of retinal degenerative diseases.


Asunto(s)
Células Fotorreceptoras de Vertebrados/efectos de la radiación , Degeneración Retiniana/prevención & control , Factor de Células Madre/fisiología , Animales , Modelos Animales de Enfermedad , Hemo-Oxigenasa 1/análisis , Luz , Proteínas de la Membrana/análisis , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Factor 2 Relacionado con NF-E2/fisiología , Proteínas Proto-Oncogénicas c-kit/fisiología , Degeneración Retiniana/etiología , Degeneración Retiniana/genética , Transducción de Señal
13.
Biochem Biophys Res Commun ; 526(2): 479-484, 2020 05 28.
Artículo en Inglés | MEDLINE | ID: mdl-32234235

RESUMEN

Exposure to blue light from light-emitting diodes (LEDs) is a source of damage for human eyes in today's modern life. Although it is well known that blue light can cause cellular damage and death, the molecular mechanism underlying this is still not fully understood. Here, we demonstrated that exposure to blue LED light increased lysosome levels and perinuclear cluster formation in 661W murine photoreceptor-derived cells. Irradiation with blue LED light promoted the nuclear transport of transcription factor EB (TFEB) and a subsequent increase in lysosomal-related gene expression. Moreover, blue LED light induced morphological changes in lysosomal structure and lysosomal membrane permeabilization (LMP). These effects were suppressed by an antioxidant, N-acetylcysteine (NAC). Finally, a calcium ion chelator, BAPTA-AM, attenuated blue LED light-induced lysosomal biogenesis and cell death. Taken together, these findings suggest that oxidative stress under blue LED light increases lysosome levels via the TFEB pathway in a calcium-dependent manner, resulting in the accumulation of damaged lysosomes and subsequently lysosomal cell death. Our results imply that lysosomal homeostasis plays a key role in the maintenance of eye function and the progression of retinal diseases.


Asunto(s)
Factores de Transcripción Básicos con Cremalleras de Leucinas y Motivos Hélice-Asa-Hélice/metabolismo , Muerte Celular/efectos de la radiación , Luz/efectos adversos , Lisosomas/efectos de la radiación , Células Fotorreceptoras de Vertebrados/efectos de la radiación , Transporte Activo de Núcleo Celular/efectos de la radiación , Animales , Línea Celular , Lisosomas/metabolismo , Ratones , Estrés Oxidativo/efectos de la radiación , Células Fotorreceptoras de Vertebrados/metabolismo
14.
Exp Eye Res ; 190: 107854, 2020 01.
Artículo en Inglés | MEDLINE | ID: mdl-31669405

RESUMEN

Death of retinal photoreceptors is the basis of prevalent blinding diseases. Since steroids might have a therapeutic role in retinal degenerations, we compared the protective effects of dexamethasone and progesterone on photoreceptor death induced by mifepristone and light exposure. Therefore, we studied the effective protection doses for each steroid in the two models. In addition, we analyzed changes in the levels of pro- and antiapoptotic molecules, glucocorticoid receptors α and ß (GRα and GRß), and rhodopsin under conditions of successful protection and photoreceptor survival. Mifepristone and light exposure selectively damaged photoreceptors. In light exposed retinas, photoreceptors mainly disappeared in the dorsotemporal region, while mifepristone produced a uniform damage. Dexamethasone and progesterone, at the same dose of 4 mg/kg/day for 2 days, preserved over 88% photoreceptor nuclei in both models. Assessment of cell death regulators showed that, in control retinas, both steroids activated BCL-XL, a prosurvival molecule, and decreased BID, a proapoptotic regulator. After steroid treatment of damaged retinas, BCL-XL, BCL2 and BAX showed characteristic patterns depending on the use of dexamethasone or progesterone on mifepristone or light exposed retinas. By contrast, BID decreased with any injury-steroid combination. Changes in GRα or GRß levels did not correlate with survival but were consistent with a mechanism of ligand induced downregulation of receptor expression. GRß might be upregulated by progesterone. Both dexamethasone and progesterone increased retinal rhodopsin stores, suggesting a link between photoreceptor protection and transduction pathways. Results show that dexamethasone and progesterone induced comparable but not identical protection responses in each model.


Asunto(s)
Dexametasona/farmacología , Glucocorticoides/farmacología , Células Fotorreceptoras de Vertebrados/efectos de los fármacos , Progesterona/farmacología , Traumatismos Experimentales por Radiación/prevención & control , Degeneración Retiniana/prevención & control , Animales , Apoptosis/efectos de los fármacos , Proteína Proapoptótica que Interacciona Mediante Dominios BH3/metabolismo , Western Blotting , Caspasa 3 , Supervivencia Celular/fisiología , Antagonistas de Hormonas/toxicidad , Inmunohistoquímica , Luz/efectos adversos , Masculino , Ratones Endogámicos BALB C , Mifepristona/toxicidad , Células Fotorreceptoras de Vertebrados/metabolismo , Células Fotorreceptoras de Vertebrados/efectos de la radiación , Traumatismos Experimentales por Radiación/etiología , Traumatismos Experimentales por Radiación/metabolismo , Receptores de Glucocorticoides/metabolismo , Degeneración Retiniana/etiología , Degeneración Retiniana/metabolismo , Rodopsina/metabolismo , Proteína bcl-X/metabolismo
15.
Adv Exp Med Biol ; 1185: 295-299, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31884627

RESUMEN

The degeneration of photoreceptors is a common hallmark of ocular diseases like retinitis pigmentosa (RP) or age-related macular degeneration (AMD). To experimentally induce photoreceptor degeneration, the light damage paradigm is frequently used. In this study we show that the exposure to high amounts of cool white light (10,000 lux, 1 h) resulted in a more than 11-fold higher apoptotic rate in the retina compared to light exposure with 5000 lux for 30 min. Consequently, exposure to intense light resulted in a significant downregulation of retinal mRNA expression levels of the reference genes Gapdh, Gnb2l, Rpl32, Rps9, Actb, Ubc or Tbp compared to untreated controls. Investigators performing light-induced photoreceptor degeneration should be aware of the fact that higher light intensities will result in a dysregulation of reference genes.


Asunto(s)
Regulación de la Expresión Génica/efectos de los fármacos , Luz , Células Fotorreceptoras de Vertebrados/efectos de la radiación , Retina/efectos de la radiación , Apoptosis , Regulación de la Expresión Génica/efectos de la radiación , Humanos , Células Fotorreceptoras de Vertebrados/patología , Retina/citología , Degeneración Retiniana/patología , Retinitis Pigmentosa/patología
16.
Proc Natl Acad Sci U S A ; 116(33): 16603-16612, 2019 08 13.
Artículo en Inglés | MEDLINE | ID: mdl-31350349

RESUMEN

Microglia respond to damage and microenvironmental changes within the central nervous system by morphologically transforming and migrating to the lesion, but the real-time behavior of populations of these resident immune cells and the neurons they support have seldom been observed simultaneously. Here, we have used in vivo high-resolution optical coherence tomography (OCT) and scanning laser ophthalmoscopy with and without adaptive optics to quantify the 3D distribution and dynamics of microglia in the living retina before and after local damage to photoreceptors. Following photoreceptor injury, microglia migrated both laterally and vertically through the retina over many hours, forming a tight cluster within the area of visible damage that resolved over 2 wk. In vivo OCT optophysiological assessment revealed that the photoreceptors occupying the damaged region lost all light-driven signaling during the period of microglia recruitment. Remarkably, photoreceptors recovered function to near-baseline levels after the microglia had departed the injury locus. These results demonstrate the spatiotemporal dynamics of microglia engagement and restoration of neuronal function during tissue remodeling and highlight the need for mechanistic studies that consider the temporal and structural dynamics of neuron-microglia interactions in vivo.


Asunto(s)
Diagnóstico por Imagen , Microglía/patología , Células Fotorreceptoras de Vertebrados/metabolismo , Células Fotorreceptoras de Vertebrados/patología , Retina/diagnóstico por imagen , Retina/lesiones , Transducción de Señal , Animales , Movimiento Celular/efectos de la radiación , Gliosis/patología , Luz , Ratones Endogámicos C57BL , Microglía/efectos de la radiación , Células Fotorreceptoras de Vertebrados/efectos de la radiación , Recuperación de la Función , Retina/fisiopatología , Retina/efectos de la radiación , Factores de Tiempo , Tomografía de Coherencia Óptica
17.
Int J Mol Sci ; 20(9)2019 May 10.
Artículo en Inglés | MEDLINE | ID: mdl-31083373

RESUMEN

Liquid crystal displays (LCDs) are used as screens in consumer electronics and are indispensable in the modern era of computing. LCDs utilize light-emitting diodes (LEDs) as backlight modules and emit high levels of blue light, which may cause retinal photoreceptor cell damage. However, traditional blue light filters may decrease the luminance of light and reduce visual quality. We adjusted the emitted light spectrum of LED backlight modules in LCDs and reduced the energy emission but maintained the luminance. The 661W photoreceptor cell line was used as the model system. We established a formula of the ocular energy exposure index (OEEI), which could be used as the indicator of LCD energy emission. Cell viability decreased and apoptosis increased significantly after exposure to LCDs with higher emitted energy. Cell damage occurred through the induction of oxidative stress and mitochondrial dysfunction. The molecular mechanisms included activation of the NF-κB pathway and upregulation of the expression of proteins associated with inflammation and apoptosis. The effect was correlated with OEEI intensity. We demonstrated that LCD exposure-induced photoreceptor damage was correlated with LCD energy emission. LCDs with lower energy emission may, therefore, serve as suitable screens to prevent light-induced retinal damage and protect consumers' eye health.


Asunto(s)
Luz , Cristales Líquidos/química , Células Fotorreceptoras de Vertebrados/patología , Células Fotorreceptoras de Vertebrados/efectos de la radiación , Animales , Apoptosis/efectos de la radiación , Caspasa 3/metabolismo , Línea Celular , Supervivencia Celular/efectos de la radiación , Inflamación/patología , Ratones , Mitocondrias/patología , Mitocondrias/efectos de la radiación , FN-kappa B/metabolismo , Estrés Oxidativo/efectos de la radiación , Exposición a la Radiación , Especies Reactivas de Oxígeno/metabolismo , Transducción de Señal/efectos de la radiación
18.
Biomaterials ; 199: 63-75, 2019 04.
Artículo en Inglés | MEDLINE | ID: mdl-30738336

RESUMEN

Tissue specific extracellular matrices (ECM) provide structural support and enable access to molecular signals and metabolites, which are essential for directing stem cell renewal and differentiation. To mimic this phenomenon in vitro, tissue decellularisation approaches have been developed, resulting in the generation of natural ECM scaffolds that have comparable physical and biochemical properties of the natural tissues and are currently gaining traction in tissue engineering and regenerative therapies due to the ease of standardised production, and constant availability. In this manuscript we report the successful generation of decellularised ECM-derived peptides from neural retina (decel NR) and retinal pigment epithelium (decel RPE), and their impact on differentiation of human pluripotent stem cells (hPSCs) to retinal organoids. We show that culture media supplementation with decel RPE and RPE-conditioned media (CM RPE) significantly increases the generation of rod photoreceptors, whilst addition of decel NR and decel RPE significantly enhances ribbon synapse marker expression and the light responsiveness of retinal organoids. Photoreceptor maturation, formation of correct synapses between retinal cells and recording of robust light responses from hPSC-derived retinal organoids remain unresolved challenges for the field of regenerative medicine. Enhanced rod photoreceptor differentiation, synaptogenesis and light response in response to addition of decellularised matrices from RPE and neural retina as shown herein provide a novel and substantial advance in generation of retinal organoids for drug screening, tissue engineering and regenerative medicine.


Asunto(s)
Biomarcadores/metabolismo , Matriz Extracelular/química , Luz , Organoides/citología , Péptidos/farmacología , Células Madre Pluripotentes/citología , Epitelio Pigmentado de la Retina/metabolismo , Sinapsis/metabolismo , Adulto , Animales , Bovinos , Diferenciación Celular/efectos de los fármacos , Medios de Cultivo Condicionados/farmacología , Matriz Extracelular/efectos de los fármacos , Matriz Extracelular/efectos de la radiación , Células Madre Embrionarias Humanas/citología , Células Madre Embrionarias Humanas/efectos de los fármacos , Células Madre Embrionarias Humanas/efectos de la radiación , Células Madre Embrionarias Humanas/ultraestructura , Humanos , Organoides/efectos de los fármacos , Organoides/efectos de la radiación , Organoides/ultraestructura , Células Fotorreceptoras de Vertebrados/citología , Células Fotorreceptoras de Vertebrados/efectos de los fármacos , Células Fotorreceptoras de Vertebrados/efectos de la radiación , Células Fotorreceptoras de Vertebrados/ultraestructura , Células Madre Pluripotentes/efectos de los fármacos , Células Madre Pluripotentes/efectos de la radiación , Sinapsis/efectos de los fármacos , Sinapsis/efectos de la radiación
19.
Mol Vis ; 25: 902-911, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-32025182

RESUMEN

Purpose: The authors previously reported that progranulin attenuated retinal degeneration. The present study focused on the role of progranulin and its cleavage products, granulins, in the pathogenesis of photoreceptor degeneration. Methods: Photoreceptor degeneration was induced with excessive exposure of murine photoreceptor cells and the retinas of albino mice to white fluorescent light. Damaged photoreceptor cells and retinas were examined using a cell death assay, western blotting, and immunostaining. Results: Even after proteolytic cleavage, treatment with progranulin or its cleavage products or both exerted protective effects on photoreceptors against light exposure. In the murine retina, the expression levels of granulins and the macrophage and microglia marker Iba-1 were increased at 48 h after light exposure. Additionally, progranulin+ and Iba-1+ double-positive cells had accumulated in the outer nuclear layer, the primary location of photoreceptor cells. Conclusions: These results suggest that progranulin or its cleavage products, granulins, or both may be therapeutic targets for age-related macular degeneration and other neurodegenerative diseases.


Asunto(s)
Granulinas/metabolismo , Células Fotorreceptoras de Vertebrados/metabolismo , Células Fotorreceptoras de Vertebrados/patología , Progranulinas/metabolismo , Degeneración Retiniana/patología , Animales , Muerte Celular/efectos de la radiación , Línea Celular , Luz , Macrófagos/efectos de los fármacos , Macrófagos/metabolismo , Macrófagos/efectos de la radiación , Ratones Noqueados , Microglía/efectos de los fármacos , Microglía/metabolismo , Microglía/efectos de la radiación , Células Fotorreceptoras de Vertebrados/efectos de los fármacos , Células Fotorreceptoras de Vertebrados/efectos de la radiación , Sustancias Protectoras/farmacología
20.
Clin Exp Ophthalmol ; 47(5): 646-657, 2019 07.
Artículo en Inglés | MEDLINE | ID: mdl-30474307

RESUMEN

BACKGROUND: This study aimed to investigate the neuroprotective function of a synthesized glucocorticoid-induced leucine zipper peptide (GILZ-p) in a light-induced retinal degeneration model. METHODS: The GILZ98-134 peptide was synthesized and injected intravitreally into Sprague Dawley rats. Retinal injury was then induced in the rats by exposing their eyes to constant white light (5000 lux) for 24 h. The activation of retinal caspases-9/3 and the release of cytochrome c from the mitochondria to the cytosol were measured at 1, 3, 5 and 7 d after light injury. Photoreceptor apoptosis was evaluated with terminal-deoxynucleotidyl-transferase-mediated deoxyuridine triphosphate-biotin nick end labelling (TUNEL) staining at 3 d after injury. Haematoxylin and eosin staining and electroretinography were used to observe the changes in the retinal morphology and function, respectively, at 7 and 14 d after light injury. RESULTS: The intravitreally injected synthesized GILZ-p successfully penetrated to the retina and significantly inhibited the activation of retinal caspase-3 and caspase-9 at 1, 3, 5 and 7 d after light injury, and reduced the number of TUNEL-positive photoreceptors at 3 d after light injury. GILZ-p pre-treatment also alleviated cytochrome c release and rescued mitochondria-mediated apoptosis after injury. Simultaneously, GILZ-p pre-treatment also mitigated the light-induced thinning of the outer nuclear layer and the loss of retinal function at 7 and 14 d after light injury, respectively. CONCLUSIONS: The synthesized GILZ-p prevented light-induced photoreceptor apoptosis and protected retinal function from degeneration, and is therefore a potential therapeutic option for degenerative retinal diseases.


Asunto(s)
Apoptosis/efectos de los fármacos , Luz/efectos adversos , Células Fotorreceptoras de Vertebrados/fisiología , Células Fotorreceptoras de Vertebrados/efectos de la radiación , Traumatismos Experimentales por Radiación/prevención & control , Degeneración Retiniana/prevención & control , Factores de Transcripción/farmacología , Animales , Western Blotting , Electrorretinografía , Células Ependimogliales/efectos de los fármacos , Etiquetado Corte-Fin in Situ , Inyecciones Intravítreas , Leucina Zippers , Masculino , Fragmentos de Péptidos/síntesis química , Traumatismos Experimentales por Radiación/etiología , Traumatismos Experimentales por Radiación/fisiopatología , Ratas , Ratas Sprague-Dawley , Degeneración Retiniana/etiología , Degeneración Retiniana/fisiopatología , Factores de Transcripción/síntesis química , Factores de Transcripción/fisiología
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