RESUMEN
AMPA-type glutamate receptors (AMPARs) mediate rapid signal transmission at excitatory synapses in the brain. Glutamate binding to the receptor's ligand-binding domains (LBDs) leads to ion channel activation and desensitization. Gating kinetics shape synaptic transmission and are strongly modulated by transmembrane AMPAR regulatory proteins (TARPs) through currently incompletely resolved mechanisms. Here, electron cryo-microscopy structures of the GluA1/2 TARP-γ8 complex, in both open and desensitized states (at 3.5 Å), reveal state-selective engagement of the LBDs by the large TARP-γ8 loop ('ß1'), elucidating how this TARP stabilizes specific gating states. We further show how TARPs alter channel rectification, by interacting with the pore helix of the selectivity filter. Lastly, we reveal that the Q/R-editing site couples the channel constriction at the filter entrance to the gate, and forms the major cation binding site in the conduction path. Our results provide a mechanistic framework of how TARPs modulate AMPAR gating and conductance.
Asunto(s)
Canales de Calcio/metabolismo , Receptores AMPA/metabolismo , Animales , Canales de Calcio/genética , Canales de Calcio/aislamiento & purificación , Canales de Calcio/ultraestructura , Microscopía por Crioelectrón , Ácido Glutámico/metabolismo , Células HEK293 , Humanos , Mutación , Técnicas de Placa-Clamp , Dominios Proteicos/genética , Ratas , Receptores AMPA/genética , Receptores AMPA/aislamiento & purificación , Receptores AMPA/ultraestructura , Proteínas Recombinantes/genética , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismo , Proteínas Recombinantes/ultraestructura , Transmisión Sináptica , TransfecciónRESUMEN
Transmembrane and coiled-coil domains 1 (TMCO1) has a highly conserved amino acid sequence among species, indicating a critical role of TMCO1 in cell physiology. The deficiency of TMCO1 in humans is associated with cerebrofaciothoracic dysplasia (CFTD), glaucoma, osteogenesis and the occurrence of cancer. TMCO1 was recently identified as an endoplasmic reticulum (ER) Ca2+ load-activated Ca2+ (CLAC) release channel, which prevents ER Ca2+ overload and maintains calcium homeostasis in the ER. However, the structural basis of the molecular function of TMCO1 channel remains elusive. To determine the structure of TMCO1, we screened the expression of TMCO1 in Escherichia coli and insect cell expression systems. TMCO1 from Dictyostelium discoideum (DdTMCO1) was successfully expressed in Escherichia coli with a high yield. The pure recombinant protein was obtained by affinity chromatography and size exclusion chromatography. The solution NMR of DdTMCO1 in DPC micelles showed three α-helical transmembrane regions.
Asunto(s)
Canales de Calcio , Proteínas Recombinantes , Animales , Canales de Calcio/química , Canales de Calcio/genética , Canales de Calcio/aislamiento & purificación , Canales de Calcio/metabolismo , Dictyostelium/genética , Escherichia coli/genética , Humanos , Resonancia Magnética Nuclear Biomolecular , Proteínas Protozoarias/química , Proteínas Protozoarias/genética , Proteínas Protozoarias/aislamiento & purificación , Proteínas Protozoarias/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismo , Células Sf9RESUMEN
Calcium (Ca2+ ) signaling controls T-cell activation and functions. Ca2+ concentrations are locally detected and controlled by Ca2+ -sensors (STIM1 and 2 detecting the depletion from ER stores channels) and Ca2+ -channels (ORAI1-3 in the cell membrane and VDAC1 in the outer mitochondrial membrane). We first validated and titrated antibodies to assess the expression of these Ca2+ -sensors and -channels in human and murine cells, and further devised a 18-antibodies mass cytometry panel to characterize their expression in primary murine lymphocyte subsets.
Asunto(s)
Canales de Calcio/aislamiento & purificación , Citometría de Flujo/métodos , Regulación de la Expresión Génica/genética , Animales , Canales de Calcio/genética , Membrana Celular/genética , Membrana Celular/metabolismo , Humanos , Subgrupos Linfocitarios/inmunología , Subgrupos Linfocitarios/metabolismo , Ratones , Membranas Mitocondriales/metabolismo , Proteína ORAI1/genética , Proteína ORAI1/aislamiento & purificación , Molécula de Interacción Estromal 1/genética , Molécula de Interacción Estromal 1/aislamiento & purificación , Molécula de Interacción Estromal 2/genética , Molécula de Interacción Estromal 2/aislamiento & purificación , Canal Aniónico 1 Dependiente del Voltaje/genéticaRESUMEN
A low-molecular-weight component (LMC) inducing selective transport of calcium across the bilayer lipid membrane has been isolated from mitochondria of the bovine heart by the method developed in our laboratory, which excludes the use of detergents and proteolytic enzymes. It was shown that, in the presence of 10 mM CaCl2, LMC forms conduction channels in the membrane multiples of 5 pS. The specific inhibitor of mitochondrial calcium uniporter, ruthenium red, closes Ca2(+)-induced channels formed in the membrane by LMC. In the absence of calcium or in the presence of potassium ions only, the component is incapable of forming channels of conduction. It was shown using nuclear magnetic resonance that LMC is a complex consisting of lipids, amino acids, and sugars with a molecular weight of 1-2 kDa.
Asunto(s)
Canales de Calcio/química , Proteínas Mitocondriales/química , Animales , Calcio/química , Bloqueadores de los Canales de Calcio/química , Canales de Calcio/aislamiento & purificación , Cationes Bivalentes , Bovinos , Transporte Iónico , Membrana Dobles de Lípidos/química , Espectroscopía de Resonancia Magnética , Proteínas Mitocondriales/aislamiento & purificación , Rojo de Rutenio/químicaRESUMEN
Inactivation of voltage-gated calcium channels is crucial for the spatiotemporal coordination of calcium signals and prevention of toxic calcium buildup. Only one member of the highly conserved family of calcium channel beta-subunits--Ca(V)beta--inhibits inactivation. This unique property has been attributed to short variable regions of the protein; however, here we report that this inhibition actually is conferred by a conserved guanylate kinase (GK) domain and, moreover, that this domain alone recapitulates Ca(V)beta-mediated modulation of channel activation. We expressed and refolded the GK domain of Ca(V)beta(2a), the unique variant that inhibits inactivation, and of Ca(V)beta(1b), an isoform that facilitates it. The refolded domains of both Ca(V)beta variants were found to inhibit inactivation of Ca(V)2.3 channels expressed in Xenopus laevis oocytes. These findings suggest that the GK domain endows calcium channels with a brake restraining voltage-dependent inactivation, and thus facilitation of inactivation by full-length Ca(V)beta requires additional structural determinants to antagonize the GK effect. We found that Ca(V)beta can switch the inactivation phenotype conferred to Ca(V)2.3 from slow to fast after posttranslational modifications during channel biogenesis. Our findings provide a framework within which to understand the modulation of inactivation and a new functional map of Ca(V)beta in which the GK domain regulates channel gating and the other conserved domain (Src homology 3) may couple calcium channels to other signaling pathways.
Asunto(s)
Canales de Calcio/química , Canales de Calcio/metabolismo , Guanilato-Quinasas/metabolismo , Activación del Canal Iónico , Animales , Canales de Calcio/genética , Canales de Calcio/aislamiento & purificación , Electrofisiología , Femenino , Guanilato-Quinasas/genética , Modelos Moleculares , Oocitos , Fenotipo , Unión Proteica , Pliegue de Proteína , Estructura Cuaternaria de Proteína , Subunidades de Proteína/química , Subunidades de Proteína/genética , Subunidades de Proteína/aislamiento & purificación , Subunidades de Proteína/metabolismo , Ratas , Factores de Tiempo , Xenopus laevisRESUMEN
Functional assays of inositol 1,4,5-trisphosphate receptors (IP3R) currently use 45Ca2+ release methods, fluorescent Ca2+ indicators within either the ER or cytosol, or electrophysiological analyses of IP3R in the nuclear envelope or artificial bilayers. None of the methods is presently amenable to the rapid, high-throughput quantitative analyses of IP3R function needed to address the structural determinants of IP3R behavior. We use a low-affinity Ca2+ indicator (Mag-fluo-4) to measure free [Ca2+] within the ER of permeabilized DT40 cells expressing only rat type 1 IP(3)R, and establish that the indicator is capable of reliably reporting the Ca(2+) release evoked by IP3. A 96-well fluorescence plate reader equipped for automated fluid additions (FlexStation, Molecular Devices) is used to monitor IP3-evoked Ca2+ release. The method allows quick and economical functional assays of recombinant IP3R in small volumes (< or = 100 microl).
Asunto(s)
Linfocitos B/metabolismo , Canales de Calcio/metabolismo , Calcio/metabolismo , Inositol 1,4,5-Trifosfato/farmacología , Receptores Citoplasmáticos y Nucleares/metabolismo , Animales , Linfocitos B/citología , Linfocitos B/efectos de los fármacos , Canales de Calcio/aislamiento & purificación , Señalización del Calcio , Pollos , Citosol/metabolismo , Retículo Endoplásmico/metabolismo , Colorantes Fluorescentes , Receptores de Inositol 1,4,5-Trifosfato , Receptores Citoplasmáticos y Nucleares/aislamiento & purificación , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismoRESUMEN
Two versions of the functional core of the rabbit voltage-dependent calcium channel beta2a subunit were expressed in Escherichia coli. These proteins were purified to homogeneity and screened for crystallization. Crystallization conditions were refined using the hanging-drop vapour-diffusion method and two crystal forms were pursued. Crystal form I is represented by thick rods with tetragonal symmetry, unit-cell parameters a = b = 75, c = 165 A and a diffraction limit of 3.4 A which were obtained using ammonium sulfate as a precipitant. Crystal form II gives rise to plates with orthorhombic symmetry, unit-cell parameters a = 35, b = 75, c = 165 A and a diffraction limit of 2.3 A which were grown using polyethylene glycol 20K as a precipitant.
Asunto(s)
Canales de Calcio/química , Canales de Calcio/aislamiento & purificación , Subunidades de Proteína/química , Subunidades de Proteína/aislamiento & purificación , Canales de Calcio/genética , Cristalización , Escherichia coli/genética , Expresión Génica , Subunidades de Proteína/genéticaRESUMEN
Cytosolic free Ca2+ serves as an important second messenger participating in signal transduction of various environmental stresses. However, molecular bases for the plasma membrane Ca2+ influx and its regulation remain largely unknown. We here identified a gene (OsTPC1) encoding a putative voltage-gated Ca2+ channel from rice, ubiquitously expressed in mature leaves, shoots and roots as well as in cultured cells. OsTPC1 rescued the Ca2+ uptake activity and growth rate of a yeast mutant cch1. To elucidate its physiological roles, we generated transgenic rice plants and cultured cells overexpressing OsTPC1 mRNA. Furthermore, a retrotransposon (Tos17) insertional knockout mutant of OsTPC1 was isolated. OsTPC1-overexpressing cells showed hypersensitivity to excess Ca2+ but higher growth rate under Ca2+ limitation, while growth of the OsTPC1-knockout cultured cells was less sensitive to extracellular free Ca2+ concentration, suggesting that OsTPC1 has Ca2+ transport activity across the plasma membrane. OsTPC1-overexpressing plants showed reduced growth and abnormal greening of roots. Growth of Ostpc1 seedlings was comparable to the control on agar plates, while significantly reduced in adult plants. These results suggest that OsTPC1 functions as a Ca2+ -permeable channel involved in the regulation of growth and development.
Asunto(s)
Canales de Calcio/metabolismo , Calcio/metabolismo , Membrana Celular/metabolismo , Oryza/crecimiento & desarrollo , Oryza/metabolismo , Proteínas de Plantas/metabolismo , Canales de Calcio/genética , Canales de Calcio/aislamiento & purificación , Señalización del Calcio/genética , Membrana Celular/genética , Células Cultivadas , Regulación del Desarrollo de la Expresión Génica/genética , Regulación de la Expresión Génica de las Plantas/genética , Datos de Secuencia Molecular , Mutación/genética , Oryza/genética , Hojas de la Planta/genética , Hojas de la Planta/crecimiento & desarrollo , Hojas de la Planta/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/aislamiento & purificación , Raíces de Plantas/genética , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/metabolismo , Plantas Modificadas Genéticamente/genética , Plantas Modificadas Genéticamente/metabolismo , ARN Mensajero/metabolismo , Plantones/genética , Plantones/crecimiento & desarrollo , Plantones/metabolismo , Homología de Secuencia de Aminoácido , Homología de Secuencia de Ácido NucleicoRESUMEN
BACKGROUND: CatSper1 and CatSper2 are two recently identified channel-like proteins, which show sperm specific expression patterns. Through targeted mutagenesis in the mouse, CatSper1 has been shown to be required for fertility, sperm motility and for cAMP induced Ca2+ current in sperm. Both channels resemble a single pore forming repeat from a four repeat voltage dependent Ca2+ /Na+ channel. However, neither CatSper1 or CatSper2 have been shown to function as cation channels when transfected into cells, singly or in conjunction. As the pore forming units of voltage gated cation channels form a tetramer it has been suggested that the known CatSper proteins require additional subunits and/or interaction partners to function. RESULTS: Using in silico gene identification and prediction techniques, we have identified two further members of the CatSper family, CatSper3 and Catsper4. Each carries a single channel-forming domain with the predicted pore-loop containing the consensus sequence TxDxW. Each of the new CatSper genes has evidence for expression in the testis. Furthermore we identified coiled-coil protein-protein interaction domains in the C-terminal tails of each of the CatSper channels, implying that CatSper channels 1,2,3 and 4 may interact directly or indirectly to form a functional tetramer. CONCLUSIONS: The topological and sequence relationship of CatSper1 and CatSper2 to the four repeat Ca2+ /Na+ channels suggested other members of this family may exist. We have identified a further two novel CatSper genes, conserved in both the human and mouse genomes. Furthermore, all four of the CatSper proteins are predicted to contain a common coiled-coil protein-protein interaction domain in their C-terminal tail. Coupled with expression data this leads to the hypothesis that the CatSper proteins form a functional hetero-tetrameric channel in sperm.
Asunto(s)
Canales de Calcio/genética , Genes , Proteínas de Plasma Seminal/genética , Secuencia de Aminoácidos , Animales , Biopolímeros , Calcio/metabolismo , Canales de Calcio/biosíntesis , Canales de Calcio/química , Canales de Calcio/aislamiento & purificación , Mapeo Cromosómico , Cromosomas Humanos Par 1/genética , Cromosomas Humanos Par 5/genética , Secuencia de Consenso , Etiquetas de Secuencia Expresada , Expresión Génica , Humanos , Canales Iónicos/química , Transporte Iónico , Masculino , Ratones , Datos de Secuencia Molecular , Familia de Multigenes , Filogenia , Conformación Proteica , Mapeo de Interacción de Proteínas , Estructura Terciaria de Proteína , ARN Mensajero/genética , Proteínas de Plasma Seminal/biosíntesis , Proteínas de Plasma Seminal/química , Proteínas de Plasma Seminal/aislamiento & purificación , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Especificidad de la Especie , Testículo/metabolismoRESUMEN
We describe a paradigm for nociception in Drosophila. In response to the touch of a probe heated above 38 degrees C, Drosophila larvae produce a stereotypical rolling behavior, unlike the response to an unheated probe. In a genetic screen for mutants defective in this noxious heat response, we identified the painless gene. Recordings from wild-type larval nerves identified neurons that initiated strong spiking above 38 degrees C, and this activity was absent in the painless mutant. The painless mRNA encodes a protein of the transient receptor potential ion channel family. Painless is required for both thermal and mechanical nociception, but not for sensing light touch. painless is expressed in peripheral neurons that extend multiple branched dendrites beneath the larval epidermis, similar to vertebrate pain receptors. An antibody to Painless binds to localized dendritic structures that we hypothesize are involved in nociceptive signaling.
Asunto(s)
Canales de Calcio/aislamiento & purificación , Proteínas de Drosophila/aislamiento & purificación , Drosophila melanogaster/metabolismo , Proteínas de Insectos/aislamiento & purificación , Canales Iónicos/aislamiento & purificación , Larva/metabolismo , Nociceptores/metabolismo , Dolor/metabolismo , Animales , Conducta Animal/fisiología , Canales de Calcio/genética , Clonación Molecular , ADN Complementario/análisis , ADN Complementario/genética , Proteínas de Drosophila/genética , Drosophila melanogaster/citología , Drosophila melanogaster/genética , Proteínas de Insectos/genética , Canales Iónicos/genética , Larva/citología , Larva/genética , Datos de Secuencia Molecular , Mutación/genética , Sistema Nervioso/citología , Sistema Nervioso/metabolismo , Neuronas Aferentes/citología , Neuronas Aferentes/metabolismo , Nociceptores/citología , Dolor/genética , Filogenia , Estimulación Física , Homología de Secuencia de Aminoácido , Homología de Secuencia de Ácido Nucleico , Canales de Potencial de Receptor TransitorioRESUMEN
The inositol 1,4,5-trisphosphate receptor (IP(3)R) is a tetrameric intracellular Ca(2+) channel, which mediates the release of Ca(2+) from the endoplasmic reticulum in response to many different extracellular stimuli. We present a 3D structure of the type 1 IP(3)R obtained by electron microscopy and single-particle analysis that reveals its domain organization. The IP(3)R has a flower-like appearance with fourfold symmetry and is made up of three distinct domains connected by slender links. By relating the organization of the structural domains to secondary-structure predictions and biochemical data we develop a model in which structural domains are mapped onto the amino acid sequence to deduce the location of the channel region and the cytoplasmic inositol 1,4,5-trisphosphate-binding and modulatory subdomains. The structure of the IP(3)R is compared with that of other tetrameric cation channels. The channel domain is similar in size and shape to its counterparts in the ryanodine receptor and the Shaker voltage-gated K(+) channel.
Asunto(s)
Canales de Calcio/química , Canales de Calcio/ultraestructura , Receptores Citoplasmáticos y Nucleares/química , Receptores Citoplasmáticos y Nucleares/ultraestructura , Animales , Canales de Calcio/aislamiento & purificación , Membrana Celular/química , Cerebelo/química , Citoplasma/ultraestructura , Procesamiento de Imagen Asistido por Computador , Receptores de Inositol 1,4,5-Trifosfato , Canales Iónicos/química , Canales Iónicos/aislamiento & purificación , Microscopía Electrónica , Conformación Proteica , Estructura Secundaria de Proteína , Subunidades de Proteína/química , Subunidades de Proteína/aislamiento & purificación , Receptores Citoplasmáticos y Nucleares/aislamiento & purificaciónRESUMEN
We report here the first three-dimensional structure of the type 1 inositol 1,4,5-trisphosphate receptor (IP(3)R). From cryo-electron microscopic images of purified receptors embedded in vitreous ice, a three-dimensional structure was determined by use of standard single particle reconstruction techniques. The structure is strikingly different from that of the ryanodine receptor at similar resolution despite molecular similarities between these two calcium release channels. The 24 A resolution structure of the IP(3)R takes the shape of an uneven dumbbell, and is approximately 170 A tall. Its larger end is bulky, with four arms protruding laterally by approximately 50 A and, in comparison with the receptor topology, probably corresponds to the cytoplasmic domain of the receptor. The lateral dimension at the height of the protruding arms is approximately 155 A. The smaller end, whose lateral dimension is approximately 100 A, has structural features indicative of the membrane-spanning domain. A central opening in this domain, which is occluded on the cytoplasmic half, outlines a pathway for calcium flow in the open state of the channel.
Asunto(s)
Canales de Calcio/química , Receptores Citoplasmáticos y Nucleares/química , Animales , Canales de Calcio/aislamiento & purificación , Canales de Calcio/metabolismo , Receptores de Inositol 1,4,5-Trifosfato , Ratones , Microscopía Electrónica/métodos , Conformación Proteica , Receptores Citoplasmáticos y Nucleares/aislamiento & purificación , Receptores Citoplasmáticos y Nucleares/metabolismo , Resonancia por Plasmón de SuperficieRESUMEN
Native high-voltage-gated calcium channels are multi-subunit complexes comprising a pore-forming subunit Ca(v) and at least two auxiliary subunits alpha(2)delta and beta. The beta subunit facilitates cell-surface expression of the channel and contributes significantly to its biophysical properties. In spite of its importance, detailed structural and functional studies are hampered by the limited availability of native beta subunit. Here, we report the purification of a recombinant calcium-channel beta(4) subunit from bacterial extracts by using a polyhistidine tag. The purified protein is fully functional since it binds on the alpha1 interaction domain, its main Ca(v)-binding site, and regulates the activity of P/Q calcium channel expressed in Xenopus oocytes in a similar way to the beta(4) subunit produced by cRNA injection. We took advantage of the functionality of the purified material to (i) develop an efficient surface-plasmon resonance assay of the interaction between two calcium channel subunits and (ii) measure, for the first time, the affinity of the recombinant His-beta(4) subunit for the full-length Ca(v)2.1 channel. The availability of this purified material and the development of a surface-plasmon resonance assay opens two immediate research perspectives: (i) drug screening programmes applied to the Ca(v)/beta interaction and (ii) crystallographic studies of the calcium-channel beta(4) subunit.
Asunto(s)
Canales de Calcio/química , Resonancia por Plasmón de Superficie/métodos , Animales , Sitios de Unión , Western Blotting , Canales de Calcio/aislamiento & purificación , ADN Complementario/metabolismo , Relación Dosis-Respuesta a Droga , Electrofisiología , Glutatión Transferasa/metabolismo , Histidina/química , Iones , Péptidos/metabolismo , Unión Proteica , Estructura Terciaria de Proteína , ARN Complementario/metabolismo , Ratas , Proteínas Recombinantes de Fusión/metabolismo , Proteínas Recombinantes/metabolismo , Factores de Tiempo , Xenopus laevisRESUMEN
This study examined the effects of quinidine, quinine, and the quaternary quinidine derivative, quinidinium, on the conductance and activity of purified cardiac sarcoplasmic reticulum calcium release channels/ryanodine receptors (RyR) incorporated into planar lipid bilayers. Quinidine (50-500 microM) reduced the single-channel open probability in a voltage- and concentration-dependent manner. Reduction of channel activity was evident only at positive holding potentials where current flow is from the cytoplasmic to luminal side of the channel and when the drug was present only on the cytoplasmic face of the channel. A more pronounced effect was the appearance of a subconductance state at positive potentials. Single channel recordings and dose-response experiments revealed that at least two quinidine molecules were involved in reduction of the RyR activity. The permanently charged quinidinium compound produced nearly identical effects as quinidine when present only on cytoplasmic side of the channel, suggesting the positive-charged form of quinidine is responsible for the effects on the channel. There was no stereospecificity in the effects of quinidine because the levoisomer, 100 microM quinine, produced a similar subconductance activity of the channel. Ryanodine modification of the channel prevented subconductance activity. These findings suggest that the quinidine-induced subconductance activity may be the result of a partial occlusion of the channel pore interfering with ion conduction. Modification of the channel by ryanodine alters quinidine binding to the channel through a conformational change in protein structure.
Asunto(s)
Canales de Calcio/metabolismo , Miocardio/metabolismo , Quinidina/farmacología , Retículo Sarcoplasmático/efectos de los fármacos , Animales , Canales de Calcio/aislamiento & purificación , Perros , Relación Dosis-Respuesta a Droga , Electrofisiología , Técnicas In Vitro , Antagonistas Muscarínicos/farmacología , Quinidina/análogos & derivados , Quinidina/química , Rianodina/metabolismo , Canal Liberador de Calcio Receptor de Rianodina/efectos de los fármacos , Canal Liberador de Calcio Receptor de Rianodina/metabolismo , Retículo Sarcoplasmático/metabolismoRESUMEN
Secretory granules of neuroendocrine cells are inositol 1,4,5-trisphosphate (InsP(3))-sensitive Ca(2+) stores in which the Ca(2+) storage protein, chromogranin A (CGA), couples with InsP(3)-gated Ca(2+) channels (InsP(3)R) located in the granule membrane. The functional aspect of this coupling has been investigated via release studies and planar lipid bilayer experiments in the presence and absence of CGA. CGA drastically increased the release activity of the InsP(3)R by increasing the channel open probability by 9-fold and the mean open time by 12-fold. Our results show that CGA-coupled InsP(3)Rs are more sensitive to activation than uncoupled receptors. This modulation of InsP(3)R channel activity by CGA appears to be an essential component in the control of intracellular Ca(2+) concentration by secretory granules and may regulate the rate of vesicle fusion and exocytosis.
Asunto(s)
Canales de Calcio/fisiología , Calcio/fisiología , Cromograninas/farmacología , Receptores Citoplasmáticos y Nucleares/fisiología , Animales , Canales de Calcio/efectos de los fármacos , Canales de Calcio/aislamiento & purificación , Bovinos , Cerebelo/fisiología , Cromogranina A , Heparina/farmacología , Receptores de Inositol 1,4,5-Trifosfato , Activación del Canal Iónico , Cinética , Membrana Dobles de Lípidos , Liposomas , Proteolípidos/metabolismo , Receptores Citoplasmáticos y Nucleares/efectos de los fármacos , Receptores Citoplasmáticos y Nucleares/aislamiento & purificaciónRESUMEN
The microsomal fraction from the log phase of Entamoeba histolytica cells contains Ins(1,4,5)P3 and Ins(1,3,4,5)P4 binding activity. The binding proteins/receptors for both Ins(1,4,5)P3 and Ins(1,3,4,5)P4 were purified and found to be specific for each ligand. The molecular masses for native proteins for InsP3 and InsP4 are 138 kDa and 130 kDa respectively having subunits of 69 kDa and 64 kDa respectively. That these proteins are associated with Ca2+ release was confirmed by including these proteins separately in proteoliposomes and adding InsP3 and InsP4 in both the cases.
Asunto(s)
Canales de Calcio/aislamiento & purificación , Entamoeba histolytica/química , Receptores Citoplasmáticos y Nucleares/aislamiento & purificación , Animales , Sitios de Unión , Calcio/metabolismo , Canales de Calcio/metabolismo , Membrana Celular/metabolismo , Inositol 1,4,5-Trifosfato/metabolismo , Receptores de Inositol 1,4,5-Trifosfato , Fosfatos de Inositol/metabolismo , Peso Molecular , Subunidades de Proteína , Proteolípidos/metabolismo , Receptores Citoplasmáticos y Nucleares/metabolismoRESUMEN
The localization of various Ca(2+) transport and signaling proteins in secretory cells is highly restricted, resulting in polarized agonist-stimulated Ca(2+) waves. In the present work, we examined the possible roles of the Sec6/8 complex or the exocyst in polarized Ca(2+) signaling in pancreatic acinar cells. Immunolocalization by confocal microscopy showed that the Sec6/8 complex is excluded from tight junctions and secretory granules in these cells. The Sec6/8 complex was found in at least two cellular compartments, part of the complex showed similar, but not identical, localization with the Golgi apparatus and part of the complex associated with Ca(2+) signaling proteins next to the plasma membrane at the apical pole. Accordingly, immunoprecipitation (IP) of Sec8 did not coimmunoprecipitate betaCOP, Golgi 58K protein, or mannosidase II, all Golgi-resident proteins. By contrast, IP of Sec8 coimmunoprecipitates Sec6, type 3 inositol 1,4,5-trisphosphate receptors (IP(3)R3), and the Gbetagamma subunit of G proteins from pancreatic acinar cell extracts. Furthermore, the anti-Sec8 antibodies coimmunoprecipitate actin, Sec6, the plasma membrane Ca(2+) pump, the G protein subunits Galphaq and Gbetagamma, the beta1 isoform of phospholipase C, and the ER resident IP(3)R1 from brain microsomal extracts. Antibodies against the various signaling and Ca(2+) transport proteins coimmunoprecipitate Sec8 and the other signaling proteins. Dissociation of actin filaments in the immunoprecipitate had no effect on the interaction between Sec6 and Sec8, but released the actin and dissociated the interaction between the Sec6/8 complex and Ca(2+) signaling proteins. Hence, the interaction between the Sec6/8 and Ca(2+) signaling complexes is likely mediated by the actin cytoskeleton. The anti-Sec6 and anti-Sec8 antibodies inhibited Ca(2+) signaling at a step upstream of Ca(2+) release by IP(3). Disruption of the actin cytoskeleton with latrunculin B in intact cells resulted in partial translocation of Sec6 and Sec8 from membranes to the cytosol and interfered with propagation of agonist-evoked Ca(2+) waves. Our results suggest that the Sec6/8 complex has multiple roles in secretory cells including governing the polarized expression of Ca(2+) signaling complexes and regulation of their activity.
Asunto(s)
Señalización del Calcio/fisiología , Calcio/metabolismo , Proteínas Portadoras/metabolismo , Páncreas/citología , Páncreas/fisiología , Actinas/fisiología , Animales , Brefeldino A/farmacología , Canales de Calcio/aislamiento & purificación , Canales de Calcio/fisiología , Señalización del Calcio/efectos de los fármacos , Carbacol/farmacología , Proteínas Portadoras/análisis , Membrana Celular/fisiología , Membrana Celular/ultraestructura , Polaridad Celular , Células Cultivadas , Citoesqueleto/fisiología , Citoesqueleto/ultraestructura , Proteínas de Unión al GTP/aislamiento & purificación , Proteínas de Unión al GTP/metabolismo , Aparato de Golgi/fisiología , Aparato de Golgi/ultraestructura , Receptores de Inositol 1,4,5-Trifosfato , Fosfatos de Inositol/farmacología , Potenciales de la Membrana/efectos de los fármacos , Potenciales de la Membrana/fisiología , Proteínas de la Membrana , Técnicas de Placa-Clamp , Ratas , Receptores Citoplasmáticos y Nucleares/aislamiento & purificación , Receptores Citoplasmáticos y Nucleares/fisiologíaRESUMEN
Using reverse transcription followed by PCR amplification (RT-PCR), we have identified multiple messenger RNAs encoding for the neuronal pore-forming Ca(2+) channel subunits alpha(1A) (P/Q channel), alpha(1B) (N channel), alpha(1D) (neuronal/endocrine L channel), alpha(1E) (R channel), alpha(1G-H) (T channel) and alpha(1S) (skeletal muscle L channel) in bovine chromaffin cells. mRNAs for the auxiliary beta(2), beta(3), beta(4), alpha(2)/delta and gamma(2) subunits were also identified. In agreement with these molecular data, perforated patch-clamp recordings of whole-cell Ca(2+) currents reveal the existence of functional R-type Ca(2+) channels in these cells that were previously undetected with other techniques. Our results provide a molecular frame for a much wider functional diversity of Ca(2+) channels in chromaffin cells than that previously established using pharmacological and electrophysiological approaches.
Asunto(s)
Canales de Calcio/clasificación , Canales de Calcio/genética , Células Cromafines/metabolismo , ARN Mensajero/aislamiento & purificación , Animales , Canales de Calcio/aislamiento & purificación , Canales de Calcio/fisiología , Bovinos , Células Cultivadas , Células Cromafines/fisiología , Técnicas de Placa-Clamp , ARN Mensajero/biosíntesis , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
To investigate the molecular mechanism of Ca transport in the kidney, we have isolated Ca-permeable channels, rECaC (rat ECaC) and mCaT (mouse CaT1), from rodent kidney, which are recently reported as Ca-transporting proteins. RT-PCR suggested the presence of CaT1 in medullary tubules. It showed 67% homology with rECaC constructing a family. Whole cellular currents in Chinese hamster ovary (CHO) cells were measured by patch clamp. Expression of both proteins exhibited a similar large cation current, a high permeability to Ca, a time-dependent rapid inactivation, and a "run-down." When the pipet contained EGTA, the inactivation and the run-down did not occur. Addition of db-cAMP activated and following rp-cAMPS recovered the mCaT-induced current significantly, whereas no influence was observed in the rECaC-induced one. We conclude that ECaC and CaT are a molecular family of ion channel with similar characteristics, contributing Ca transport in the kidney.
Asunto(s)
Canales de Calcio/metabolismo , AMP Cíclico/análogos & derivados , Riñón/metabolismo , Animales , Bucladesina/farmacología , Células CHO , Calcio/metabolismo , Canales de Calcio/genética , Canales de Calcio/aislamiento & purificación , Permeabilidad de la Membrana Celular/efectos de los fármacos , Clonación Molecular , Cricetinae , AMP Cíclico/farmacología , Ácido Egtácico/farmacología , Técnicas In Vitro , Potenciales de la Membrana/efectos de los fármacos , Ratones , Datos de Secuencia Molecular , Técnicas de Placa-Clamp , Ratas , Ratas Sprague-Dawley , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Homología de Secuencia de Aminoácido , Canales Catiónicos TRPV , Tionucleótidos/farmacología , TransfecciónRESUMEN
The mutant L-type calcium channel alpha(1)-subunit gene, CACNA1F, was recently identified as the gene responsible for incomplete X-linked congenital stationary night blindness. The 6070-bp mRNA transcript is predicted to encode a 1977-amino-acid pore-forming protein with cytoplasmic amino- and carboxyl-termini separated by four homologous repeat domains, each consisting of six transmembrane segments. CACNA1F has been shown to be preferentially expressed in the retina, indicative of a specific functional role in visual processing. We have established the complete sequence of the murine orthologue of CACNA1F, namely Cacna1f. The total length of the mRNA transcript of the murine gene was established to be 6080 bp with an open reading frame that translates into a 1985-amino-acid protein. Cacna1f is highly homologous to the human sequence, with 90% identity at the amino acid level and almost perfect conservation between the functional domains. Furthermore, as in the human gene, the 3' end of the Cacna1f gene maps within 5 kb of the 5' end of the mouse synaptophysin gene in a region orthologous to Xp11.23. Using in situ hybridization, Cacna1f was found to be expressed in the inner and outer nuclear layers and the ganglion cell layer of the retina.