Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 5.429
Filtrar
1.
Sci Rep ; 14(1): 25184, 2024 10 24.
Artículo en Inglés | MEDLINE | ID: mdl-39448759

RESUMEN

This study was performed on Micropterus salmoides to determine growth-linked gene and single nucleotide polymorphism (SNP) markers under carbohydrate diet and varying temperature through RNA-seq and whole-genome re-sequencing. The results showed that growth-related genes were primarily enriched in the fat digestion and absorption signaling pathway, playing a role in lipid transport and metabolism. Fatty acid binding protein 6, bile salt-activated lipase-like, lysophosphatidylcholine acyltransferase 2, phospholipase A2, minor isoenzyme-like, and phospholipase A2, group IB (pancreas) were identified as the crucial genes. The differentially expressed genes between high and low temperatures were enriched in the pentose phosphate pathway, with carbohydrate transport and metabolism were most affected by temperature. Major facilitator superfamily domain containing 10, phosphogluconate dehydrogenase, fructose-1,6-bisphosphatase 1a, and spinster homolog 3 transcript variant X1(spns3) were the shared genes affected by temperature. From all the common genes, 10 growth-associated SNP markers were identified. The TT genotype at rs7781 was associated with lower body weight, while AA genotype at rs31434173 and CC genotype at rs31435313 showed positive correlation with body weight. Analogously, the GG genotype at rs31436887 and AA genotype at rs31438769 also found to characterize better growth performance. At low temperature, individuals with the AA genotype at rs11506587 and rs31435313 exhibited the slowest growth. For the genotypes labeled with rs11510589, the GG individual grew faster than the AA individual, whereas the opposite phenomenon occurred in these genotypes when labeled with rs11511314. The genotypes at rs32970704 and rs32967921 showed no growth correlation. The AA genotype at rs31435313 in spns3 had the slowest growth under a carbohydrate-rich diet regardless of the temperature. Our study presents candidate genes and SNP markers associated with growth influenced by carbohydrate and temperature, providing basis for the development of M. salmoides strain that better accepts carbohydrate diets at varying temperature.


Asunto(s)
Polimorfismo de Nucleótido Simple , Temperatura , Animales , Carbohidratos de la Dieta/metabolismo , RNA-Seq , Secuenciación Completa del Genoma , Genotipo
2.
Compr Rev Food Sci Food Saf ; 23(6): e70046, 2024 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-39437196

RESUMEN

Clarification is required when the term "carbohydrate" is used interchangeably with "saccharide" and "glycan." Carbohydrate classification based on human digestive enzyme activities brings clarity to the energy supply function of digestible sugars and starch. However, categorizing structurally diverse non-digestible carbohydrates (NDCs) to make dietary intake recommendations for health promotion remains elusive. In this review, we present a summary of the strengths and weaknesses of the traditional dichotomic classifications of carbohydrates, which were introduced by food chemists, nutritionists, and microbiologists. In parallel, we discuss the current consensus on commonly used terms for NDCs such as "dietary fiber," "prebiotics," and "fermentable glycans" and highlight their inherent differences from the perspectives of gut microbiome. Moreover, we provide a historical perspective on the development of novel concepts such as microbiota-accessible carbohydrates, microbiota-directed fiber, targeted prebiotics, and glycobiome. Crucially, these novel concepts proposed by multidisciplinary scholars help to distinguish the interactions between diverse NDCs and the gut microbiome. In summary, the term NDCs created based on the inability of human digestive enzymes fails to denote their interactions with gut microbiome. Considering that the gut microbiome possesses sophisticated enzyme systems to harvest diverse NDCs, the subclassification of NDCs should be realigned to their metabolism by various gut microbes, particularly health-promoting microbes. Such rigorous categorizations facilitate the development of microbiome-targeted therapeutic strategies by incorporating specific types of NDCs.


Asunto(s)
Microbioma Gastrointestinal , Microbioma Gastrointestinal/fisiología , Humanos , Prebióticos , Carbohidratos de la Dieta/metabolismo , Fibras de la Dieta/metabolismo , Polisacáridos/metabolismo , Polisacáridos/química , Digestión/fisiología
3.
Clin Nutr ; 43(10): 2305-2315, 2024 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-39226718

RESUMEN

BACKGROUND: We have previously demonstrated that dietary saturated fatty acids (SFA), when compared to polyunsaturated fatty acids (PUFA), are preferentially partitioned into oxidation pathways. However, it remains unclear if this preferential handling is maintained when hepatocellular metabolism is shifted toward fatty acid (FA) esterification and away from oxidation, such as when hepatic de novo lipogenesis (DNL) is upregulated. AIM: To investigate whether an acute upregulation of hepatic DNL influences dietary FA partitioning into oxidation pathways. METHODS: 20 healthy volunteers (11 females) underwent a fasting baseline visit followed by two study days, 2-weeks apart. Prior to each study day, participants consumed an isocaloric high-carbohydrate diet (to upregulate hepatic DNL) for 3-days. On the two study days, participants consumed an identical standardised test meal that contained either [U13C]palmitate or [U13C]linoleate, in random order, to trace the fate of dietary FA. Blood and breath samples were collected over a 6h postprandial period and 13C enrichment in breath CO2 and plasma lipid fractions were measured using gas-chromatography-combustion-isotope ratio mass spectrometry. RESULTS: Compared to the baseline visit, fasting plasma triglyceride concentrations and markers of hepatic DNL, the lipogenic and stearyl-CoA desaturase indices, were significantly (p < 0.05) increased after consumption of the high-carbohydrate diet. Appearance of 13C in expired CO2 and tracer recovery were significantly (p < 0.05) higher after consumption of the meal containing [U13C]linoleate compared to [U13C]palmitate (5.1 ± 0.5% vs. 3.7 ± 0.4%), respectively. Incorporation of 13C into the plasma triglyceride and non-esterified fatty acid pool was significantly (p < 0.001) greater for [U13C]palmitate compared to [U13C]linoleate. CONCLUSION: Dietary PUFA compared to SFA appear to be preferentially partitioned into oxidation pathways during an acute upregulation of hepatic DNL, thus consumption of a PUFA-enriched diet may help mitigate intrahepatic triglyceride accumulation in individuals at risk of cardiometabolic disease.


Asunto(s)
Oxidación-Reducción , Palmitatos , Humanos , Femenino , Masculino , Adulto , Adulto Joven , Palmitatos/metabolismo , Ácido Linoleico/administración & dosificación , Periodo Posprandial , Hígado/metabolismo , Triglicéridos/sangre , Carbohidratos de la Dieta/administración & dosificación , Carbohidratos de la Dieta/metabolismo , Lipogénesis , Dieta de Carga de Carbohidratos , Grasas de la Dieta/administración & dosificación , Grasas de la Dieta/metabolismo
4.
Physiol Rep ; 12(16): e70020, 2024 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-39187400

RESUMEN

Modulation of testing conditions such as resting lactate (Larest) levels or carbohydrate intake may affect the calculation of the maximal glycolytic rate (νLa.max). To evaluate the impact of elevated Larest as well as reduced and increased carbohydrate availability on νLa.max in running sprints (RST), twenty-one participants completed five 15-s RST tests on a running track under five different conditions: (I). baseline: Larest ≤1.5 mmol·L-1; (II). Lactate+: Larest ≥2.5 mmol·L-1; (III). CHO-: carbohydrate intake: ≤ 1 g·kg-1 BW d-1 for 3 days; (IV). CHO+: carbohydrate intake: ≥ 9 g·kg-1 BW d-1 for one day; and (V). acuteCHO: 500 mL glucose containing beverage consumed before RST. νLa.max was significantly reduced in lactate+ and CHO- conditions compared to the baseline RST, due to a reduction in the arithmetic mean delta (∆) between Lapeak and Larest lactate concentration (Lapeak, mmol · L-1). AcuteCHO led to an increase in Larest compared to baseline, CHO- and CHO+ with a high interindividual variability but did not significantly reduce νLa.max. Therefore, avoiding low carbohydrate nutrition before νLa.max testing, along with carefully adjusting Larest to below ≤1.5 mmol·L-1, is crucial to prevent the unintentional underestimation of νLa.max.


Asunto(s)
Carbohidratos de la Dieta , Ácido Láctico , Humanos , Masculino , Ácido Láctico/metabolismo , Ácido Láctico/sangre , Proyectos Piloto , Femenino , Carbohidratos de la Dieta/administración & dosificación , Carbohidratos de la Dieta/metabolismo , Adulto , Adulto Joven , Carrera/fisiología , Glucólisis/fisiología , Descanso/fisiología
5.
Clin Nutr ; 43(9): 2106-2115, 2024 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-39111049

RESUMEN

BACKGROUND & AIMS: In recent times, the complexity of food styles and meal content has increased, leading to significant variations in macronutrient composition between meals. This phenomenon has coincided with a rise in obesity rates. We aimed to determine whether a large variation in macronutrient composition between meals results in reduced fat oxidation. METHODS: A cross-over study was conducted with 13 healthy young men, using whole-body indirect calorimetry to test 24-h energy metabolic responses under three conditions: regular meals (R), high-carbohydrate breakfast (CB), or high-fat breakfast (FB), each with different macronutrient contents. The R condition included three meals daily with the same macronutrient composition. The CB condition included a high-carbohydrate meal at breakfast, high-fat meal at lunch, and high-carbohydrate meal at dinner. The FB condition included a high-fat meal at breakfast, high-carbohydrate meal at lunch, and high-carbohydrate meal at dinner. The daily macronutrient compositions were similar across the three conditions, except that CB and FB had larger variations in carbohydrate-fat balance between meals than R. The participants were tested in random order. During the dietary intervention, we compared 24-h whole-body metabolic parameters, including substrate oxidation (e.g., 24 h respiratory quotient [RQ]). RESULTS: No significant differences were observed in the measures of energy expenditure among the three conditions. However, after adjusting for the sleeping RQ on a preceding day, the estimated 24hRQ was lower under the FB condition (0.845) than under the R (0.854, P = 0.0077 vs. FB) and CB conditions (0.853, P = 0.016 vs. FB). No difference was observed in the magnitude of the 5-h RQ change from lunch to dinner under the CB condition and in the magnitude of change from breakfast to lunch under the FB condition. CONCLUSIONS: A large variation in the carbohydrate-fat balance between meals does not decrease daily fat oxidation. An FB may increase daily fat oxidation compared to a CB when the daily food quotient is constant, but this increase may not be owing to the upregulation of fat burning on a daily basis.


Asunto(s)
Calorimetría Indirecta , Estudios Cruzados , Carbohidratos de la Dieta , Grasas de la Dieta , Metabolismo Energético , Oxidación-Reducción , Humanos , Masculino , Carbohidratos de la Dieta/administración & dosificación , Carbohidratos de la Dieta/metabolismo , Grasas de la Dieta/administración & dosificación , Adulto Joven , Adulto , Metabolismo Energético/fisiología , Comidas/fisiología , Ritmo Circadiano/fisiología , Nutrientes , Desayuno/fisiología , Dieta/métodos , Ingestión de Energía/fisiología
6.
Poult Sci ; 103(10): 104092, 2024 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-39116662

RESUMEN

The growth of broiler chickens is marked by high fluctuations, varying nutrient requirement, early growth is characterized by high allometric growth rates of supply organs, which if underdeveloped, can impede nutrient efficiency and growth of demand organs like muscle and skeleton. This study aimed to investigate the impact of carbohydrate- and amino-acid-rich diets on the development of supply organs in broiler chickens. Four dietary treatments were used in a 2 × 2 factorial arrangement of treatments with apparent metabolizable energy (AME) at 2 levels (low: 2,750 kcal/kg and high: 3,050 kcal/kg) and standardized ileal digestible (SID) lysine at 2 levels (low: 1.0% and high: 1.2%) in the starter diets. Feed intake (FI) and BW gain were measured weekly; dissections were conducted at d 4 and d 11 to determine supply organ weights. Allometric growth of the liver was higher (P < 0.001) in the high AME and low lysine group compared to the other groups. For the pancreas, the highest (P < 0.001) allometric growth rate was in the high lysine groups. The small intestines responded differently; the duodenum had the highest (P < 0.001) allometric growth rate in the high AME groups and the jejunum in the low lysine groups, whereas the ileum showed an effect of diet density. For performance, high AME from carbohydrates, via maize starch, had a negative effect (P < 0.001) on FI and BW gain. High lysine had a positive effect (P < 0.001) on BW gain and FI, and high lysine alleviated part of the detrimental effect of high AME from carbohydrates. This effect was visible from d 0 to d 11, and persisted till the end of the trial on d 35. In conclusion, feeding a diet with a high AME from carbohydrates has negative consequences for the development of the supply organs of broilers.


Asunto(s)
Aminoácidos , Alimentación Animal , Fenómenos Fisiológicos Nutricionales de los Animales , Pollos , Dieta , Carbohidratos de la Dieta , Animales , Pollos/crecimiento & desarrollo , Pollos/fisiología , Alimentación Animal/análisis , Dieta/veterinaria , Carbohidratos de la Dieta/administración & dosificación , Carbohidratos de la Dieta/metabolismo , Aminoácidos/metabolismo , Fenómenos Fisiológicos Nutricionales de los Animales/efectos de los fármacos , Masculino , Distribución Aleatoria
7.
Food Funct ; 15(15): 7920-7935, 2024 Jul 29.
Artículo en Inglés | MEDLINE | ID: mdl-38979640

RESUMEN

Early dietary patterns potentially influence the health status and lifespan throughout adulthood and the entire lifespan. However, dietary behaviors are difficult for everyone to control during adolescence. It is even more important to study the effects of interventions of early dietary patterns on the lifespan under arbitrary feeding conditions. The research involves observing the survival status and lifespan of rats from weaning to adulthood with three different dietary patterns (a high-carbohydrate diet (HC), a high-protein diet (HP), and a high-fat diet (HF)) under ad libitum feeding conditions. The administration of high-carbohydrate diets leads to a significant extension of both median and maximum survival times (P < 0.05) in Wistar rats. Furthermore, it markedly enhanced the spatial memory capacity, mitigated the occurrence of liver and kidney pathological outcomes in elderly rats, and increased the abundance of gut microbiota improving amino acid metabolism. Additionally, feeding rats a high-carbohydrate diet improved glutathione (GSH) synthesis and recycling and activated the expression and upregulation of the lifespan-related proteins Foxo3a/Sirt3 and the key metabolic enzyme GPX-4. The high-carbohydrate diet from weaning to adulthood may potentially extend the lifespan by enhancing rat systemic glutathione synthesis, recycling, and improving the redox state pathway.


Asunto(s)
Homeostasis , Longevidad , Oxidación-Reducción , Ratas Wistar , Destete , Animales , Ratas , Masculino , Microbioma Gastrointestinal , Carbohidratos de la Dieta/metabolismo , Hígado/metabolismo , Glutatión/metabolismo , Dieta Alta en Grasa , Multiómica
8.
Mol Nutr Food Res ; 68(14): e2300555, 2024 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-39059012

RESUMEN

SCOPE: There is a lack of research comparing how different protein isolates influence the microbiome, especially when carbohydrate (CHO) availability is varied. The objective is to determine changes in gut microbiota composition and function during fermentation of digested protein isolates under high and low CHO conditions. METHODS AND RESULTS: Protein isolates from beef, egg white, milk, pea, and soy are subjected to in vitro digestion and fermentation with human fecal microbiota. Under low CHO conditions, the microbiota is primarily proteolytic with decreased concentrations of peptides and increased variance among microbial taxa and production of ammonia and branched chain fatty acids by the microbiota. Milk protein not only results in the highest production of butyrate and p-hydroxyphenylacetate but also has high concentrations of deleterious fermentation metabolites. Amino acid composition of the protein isolates is significantly correlated with abundances of many microbial taxa and metabolites, but the correlations are stronger in the low CHO medium. CONCLUSION: This study shows that low CHO conditions increase proteolytic fermentation and result in increased differences in microbiota response to protein isolates. It also showed that amino acid composition is highly associated with microbiota composition and function especially under low CHO conditions.


Asunto(s)
Heces , Fermentación , Microbioma Gastrointestinal , Humanos , Microbioma Gastrointestinal/fisiología , Microbioma Gastrointestinal/efectos de los fármacos , Animales , Heces/microbiología , Aminoácidos/metabolismo , Carbohidratos de la Dieta/metabolismo , Femenino , Proteínas en la Dieta/metabolismo , Adulto , Masculino
9.
J Insect Physiol ; 157: 104684, 2024 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-39074715

RESUMEN

Crickets have been extensively studied in recent insect nutritional research, but it remains largely unexplored how they balance the intake of multiple nutrients. Here, we used the nutritional geometry framework to examine the behavioural and physiological regulation of dietary protein and carbohydrate in nymphs of the two-spotted cricket, Gryllus bimaculatus (Orthoptera: Gryllidae). Growth, intake, utilization efficiencies, and body composition were measured from the eighth instar nymphs that received either food pairs or single foods with differing protein and carbohydrate content. When food choices were available, crickets preferentially selected a carbohydrate-biased protein:carbohydrate (P:C) ratio of 1:1.74. During this nutrient selection, carbohydrate intake was more tightly regulated than protein intake. When confined to nutritionally imbalanced foods, crickets adopted a nutrient balancing strategy that maximized the nutrient intake regardless of the nutrient imbalance, reflecting their omnivorous feeding habit. Intake was significantly reduced when crickets were confined to the most carbohydrate-biased food (P:C = 1:5). When nutrients were ingested in excess of the requirements, the post-ingestive utilization efficiencies of these nutrients were down-regulated, thereby buffering the impacts of nutrient imbalances on body nutrient composition. Crickets reared on the most carbohydrate-biased food (P:C = 1:5) suffered delayed development and reduced growth. Our data provide the most accurate description of nutrient regulation in G. bimaculatus and lay the foundation for further nutritional research in this omnivorous insect.


Asunto(s)
Gryllidae , Ninfa , Animales , Gryllidae/crecimiento & desarrollo , Gryllidae/fisiología , Ninfa/crecimiento & desarrollo , Ninfa/fisiología , Carbohidratos de la Dieta/metabolismo , Fenómenos Fisiológicos Nutricionales de los Animales , Proteínas en la Dieta/metabolismo , Conducta Alimentaria , Nutrientes/metabolismo , Composición Corporal
10.
Hypertension ; 81(9): 1966-1975, 2024 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-39005213

RESUMEN

BACKGROUND: The blood pressure (BP) etiologic study is complex due to multifactorial influences, including genetic, environmental, lifestyle, and their intricate interplays. We used a metabolomics approach to capture internal pathways and external exposures and to study BP regulation mechanisms after well-controlled dietary interventions. METHODS: In the ProBP trail (Protein and Blood Pressure), a double-blinded crossover randomized controlled trial, participants underwent dietary interventions of carbohydrate, soy protein, and milk protein, receiving 40 g daily for 8 weeks, with 3-week washout periods. We measured plasma samples collected at baseline and at the end of each dietary intervention. Multivariate linear models were used to evaluate the association between metabolites and systolic/diastolic BP. Nominally significant metabolites were examined for enriching biological pathways. Significant ProBP findings were evaluated for replication among 1311 participants of the BHS (Bogalusa Heart Study), a population-based study conducted in the same area as ProBP. RESULTS: After Bonferroni correction for 77 independent metabolite clusters (α=6.49×10-4), 18 metabolites were significantly associated with BP at baseline or the end of a dietary intervention, of which 11 were replicated in BHS. Seven emerged as novel discoveries, which are as follows: 1-linoleoyl-GPE (18:2), 1-oleoyl-GPE (18:1), 1-stearoyl-2-linoleoyl-GPC (18:0/18:2), 1-palmitoyl-2-oleoyl-GPE (16:0/18:1), maltose, N-stearoyl-sphinganine (d18:0/18:0), and N6-carbamoylthreonyladenosine. Pathway enrichment analyses suggested dietary protein intervention might reduce BP through pathways related to G protein-coupled receptors, incretin function, selenium micronutrient network, and mitochondrial biogenesis. CONCLUSIONS: Seven novel metabolites were identified to be associated with BP at the end of different dietary interventions. The beneficial effects of protein interventions might be mediated through specific metabolic pathways.


Asunto(s)
Presión Sanguínea , Estudios Cruzados , Hipertensión , Humanos , Masculino , Femenino , Presión Sanguínea/fisiología , Método Doble Ciego , Hipertensión/dietoterapia , Hipertensión/fisiopatología , Persona de Mediana Edad , Adulto , Metabolómica/métodos , Carbohidratos de la Dieta/metabolismo
11.
Curr Opin Clin Nutr Metab Care ; 27(4): 338-343, 2024 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-38836807

RESUMEN

PURPOSE OF REVIEW: This review will summarize recent studies assessing the effect of slowly digestible starch (SDS) and resistant starch (RS) on glucose metabolism in healthy, prediabetic or type 2 diabetic adults. RECENT FINDINGS: Currently, a particular interest in starch and its digestibility has arisen, with data showing a positive effect of SDS and RS on the glucose homeostasis of healthy, at-risk, prediabetic and type 2 diabetic patients but research is ongoing. SUMMARY: Carbohydrates (CHO) and especially starch play a major role in the prevention and management of metabolic diseases such as type 2 diabetes (T2D). This largely depends on the quality and the digestibility (rate and extent) of the ingested starchy products, beyond their quantity. SDS have been poorly studied but display a beneficial effect on reducing glucose excursions in healthy and insulin-resistant subjects and a relevant potential to improve glucose control in type 2 diabetic individuals. As for RS, the results appear to be encouraging but remain heterogeneous, depending the nature of the RS and its role on microbiota modulation. Further studies are needed to confirm the present results and investigate the potential complementary beneficial effects of SDS and RS on long-term glucose homeostasis to prevent cardiometabolic diseases.


Asunto(s)
Glucemia , Diabetes Mellitus Tipo 2 , Digestión , Homeostasis , Resistencia a la Insulina , Almidón , Humanos , Almidón/metabolismo , Digestión/efectos de los fármacos , Glucemia/metabolismo , Estado Prediabético/metabolismo , Almidón Resistente/farmacología , Carbohidratos de la Dieta/metabolismo
12.
Nutrients ; 16(12)2024 Jun 14.
Artículo en Inglés | MEDLINE | ID: mdl-38931241

RESUMEN

It is a common belief amongst strength and power athletes that nutritional supplementation strategies aid recovery by shifting the anabolic/catabolic profile toward anabolism. Factors such as nutrient quantity, nutrient quality, and nutrient timing significantly impact upon the effectiveness of nutritional strategies in optimizing the acute responses to resistance exercise and the adaptive response to resistance training (i.e., muscle growth and strength expression). Specifically, the aim of this review is to address carbohydrates (CHOs), protein (PRO), and/or amino acids (AAs) supplementation strategies, as there is growing evidence suggesting a link between nutrient signaling and the initiation of protein synthesis, muscle glycogen resynthesis, and the attenuation of myofibrillar protein degradation following resistance exercise. Collectively, the current scientific literature indicates that nutritional supplementation strategies utilizing CHO, PRO, and/or AA represents an important approach aimed at enhancing muscular responses for strength and power athletes, primarily increased muscular hypertrophy and enhanced strength expression. There appears to be a critical interaction between resistance exercise and nutrient-cell signaling associated with the principle of nutrient timing (i.e., pre-exercise, during, and post-exercise). Recommendations for nutritional supplementation strategies to promote muscular responses for strength and athletes are provided.


Asunto(s)
Aminoácidos , Atletas , Carbohidratos de la Dieta , Proteínas en la Dieta , Suplementos Dietéticos , Fuerza Muscular , Músculo Esquelético , Entrenamiento de Fuerza , Fenómenos Fisiológicos en la Nutrición Deportiva , Humanos , Proteínas en la Dieta/administración & dosificación , Carbohidratos de la Dieta/administración & dosificación , Carbohidratos de la Dieta/metabolismo , Aminoácidos/metabolismo , Aminoácidos/administración & dosificación , Músculo Esquelético/metabolismo , Músculo Esquelético/fisiología , Fuerza Muscular/efectos de los fármacos
13.
Am J Clin Nutr ; 120(2): 283-293, 2024 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-38914224

RESUMEN

BACKGROUND: Intrahepatic triacylglycerol (liver TG) content is associated with hepatic insulin resistance and dyslipidemia. Liver TG content can be modulated within days under hypocaloric conditions. OBJECTIVES: We hypothesized that 4 d of eucaloric low-carbohydrate/high-fat (LC) intake would decrease liver TG content, whereas a high-carbohydrate/low-fat (HC) intake would increase liver TG content, and further that alterations in liver TG would be linked to dynamic changes in hepatic glucose and lipid metabolism. METHODS: A randomized crossover trial in males with 4 d + 4 d of LC and HC, respectively, with ≥2 wk of washout. 1H-magnetic resonance spectroscopy (1H-MRS) was used to measure liver TG content, with metabolic testing before and after intake of an LC diet (11E% carbohydrate corresponding to 102 ± 12 {mean ± standard deviation [SD]) g/d, 70E% fat} and an HC diet (65E% carbohydrate corresponding to 537 ± 56 g/d, 16E% fat). Stable [6,6-2H2]-glucose and [1,1,2,3,3-D5]-glycerol tracer infusions combined with hyperinsulinemic-euglycemic clamps and indirect calorimetry were used to measure rates of hepatic glucose production and lipolysis, whole-body insulin sensitivity and substrate oxidation. RESULTS: Eleven normoglycemic males with overweight or obesity (BMI 31.6 ± 3.7 kg/m2) completed both diets. The LC diet reduced liver TG content by 35.3% (95% confidence interval: -46.6, -24.1) from 4.9% [2.4-11.0] (median interquartile range) to 2.9% [1.4-6.9], whereas there was no change after the HC diet. After the LC diet, fasting whole-body fat oxidation and plasma beta-hydroxybutyrate concentration increased, whereas markers of de novo lipogenesis (DNL) diminished. Fasting plasma TG and insulin concentrations were lowered and the hepatic insulin sensitivity index increased after LC. Peripheral glucose disposal was unchanged. CONCLUSIONS: Reduced carbohydrate and increased fat intake for 4 d induced a marked reduction in liver TG content and increased hepatic insulin sensitivity. Increased rates of fat oxidation and ketogenesis combined with lower rates of DNL are suggested to be responsible for lowering liver TG. This trial was registered at clinicaltrials.gov as NCT04581421.


Asunto(s)
Estudios Cruzados , Hígado , Obesidad , Sobrepeso , Triglicéridos , Humanos , Masculino , Triglicéridos/metabolismo , Hígado/metabolismo , Adulto , Sobrepeso/metabolismo , Sobrepeso/dietoterapia , Obesidad/metabolismo , Obesidad/dietoterapia , Dieta Baja en Carbohidratos , Carbohidratos de la Dieta/administración & dosificación , Carbohidratos de la Dieta/metabolismo , Adulto Joven , Persona de Mediana Edad , Resistencia a la Insulina
14.
Nutr Bull ; 49(3): 372-382, 2024 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-38940372

RESUMEN

While there are data regarding the glycaemic index (GI) and glycaemic load (GL) of many foods in the literature, the values for packaged vegan analogue foods have not been previously published, although processed vegan foods usually contain more carbohydrates than their animal-based counterparts. This study was carried out to determine the GI and GL values of a selection of packaged vegan foods popular in Türkiye. To determine the GI and GL of test foods, 12 healthy volunteer females participated in the study. Participants randomly consumed test and reference foods (glucose and white bread) on each trial day, and capillary blood glucose was measured at baseline, 15, 30, 45, 60, 90 and 120 mins in duplicate. The GIs of vegan schnitzel, vegan chickpea burger, vegan mince pita, vegan chocolate, vegan snack bar and vegan cheese were 26.1 ± 19.61 (low), 27.1 ± 17.21 (low), 65.1 ± 28.60 (moderate), 42.7 ± 22.32 (low), 63.6 ± 45.86 (moderate), 36.4 ± 16.85 (low), respectively, according to the glucose reference, and were 28.2 ± 16.17 (low), 34.7 ± 18.26 (low), 81.2 ± 31.96 (high), 48.8 ± 12.87 (low), 82.0 ± 54.05 (high), 46.7 ± 28.66 (low), respectively, according to the white bread reference. GLs were 3.5 ± 2.66 (low), 8.6 ± 5.46 (low), 26.6 ± 11.67 (high), 6.1 ± 3.19 (low), 14.2 ± 10.28 (moderate), 5.7 ± 2.63 (low), respectively, according to the glucose reference, and were 3.8 ± 2.19 (low), 11.0 ± 5.80 (moderate), 33.1 ± 13.04 (high), 7.0 ± 1.84 (low), 18.4 ± 12.12 (moderate), 7.3 ± 4.47 (low), respectively, according to the white bread reference. The data from this study provide preliminary data for the GIs and GLs of packaged and processed vegan foods and show that the GIs and GLs of meat and cheese vegan analogues, while still low or moderate, tend to be higher than their animal-based counterparts which have lower or no glycaemic responses. Further research on the GIs and GLs of more vegan foods is needed.


Asunto(s)
Glucemia , Dieta Vegana , Índice Glucémico , Carga Glucémica , Veganos , Humanos , Femenino , Adulto , Glucemia/análisis , Glucemia/metabolismo , Adulto Joven , Dieta Vegetariana , Embalaje de Alimentos , Pan/análisis , Carbohidratos de la Dieta/metabolismo
15.
Obesity (Silver Spring) ; 32(5): 949-958, 2024 05.
Artículo en Inglés | MEDLINE | ID: mdl-38650517

RESUMEN

OBJECTIVE: We investigated how changes in 24-h respiratory exchange ratio (RER) and substrate oxidation during fasting versus an energy balance condition influence subsequent ad libitum food intake. METHODS: Forty-four healthy, weight-stable volunteers (30 male and 14 female; mean [SD], age 39.3 [11.0] years; BMI 31.7 [8.3] kg/m2) underwent 24-h energy expenditure measurements in a respiratory chamber during energy balance (50% carbohydrate, 30% fat, and 20% protein) and 24-h fasting. Immediately after each chamber stay, participants were allowed 24-h ad libitum food intake from computerized vending machines. RESULTS: Twenty-four-hour RER decreased by 9.4% (95% CI: -10.4% to -8.5%; p < 0.0001) during fasting compared to energy balance, reflecting a decrease in carbohydrate oxidation (mean [SD], -2.6 [0.8] MJ/day; p < 0.0001) and an increase in lipid oxidation (2.3 [0.9] MJ/day; p < 0.0001). Changes in 24-h RER and carbohydrate oxidation in response to fasting were correlated with the subsequent energy intake such that smaller decreases in fasting 24-h RER and carbohydrate oxidation, but not lipid oxidation, were associated with greater energy intake after fasting (r = 0.31, p = 0.04; r = 0.40, p = 0.007; and r = -0.27, p = 0.07, respectively). CONCLUSIONS: Impaired metabolic flexibility to fasting, reflected by an inability to transition away from carbohydrate oxidation, is linked with increased energy intake.


Asunto(s)
Ingestión de Energía , Metabolismo Energético , Ayuno , Humanos , Femenino , Masculino , Adulto , Metabolismo Energético/fisiología , Persona de Mediana Edad , Voluntarios Sanos , Oxidación-Reducción , Carbohidratos de la Dieta/administración & dosificación , Carbohidratos de la Dieta/metabolismo , Metabolismo de los Lípidos/fisiología , Ingestión de Alimentos/fisiología , Índice de Masa Corporal
16.
Appl Physiol Nutr Metab ; 49(8): 1035-1046, 2024 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-38621297

RESUMEN

Our prior results showed that an acute bout of endurance exercise for 6 h, but not 1 h, decreased pancreatic amylase activity, indicating that acute endurance exercise may affect carbohydrate digestive capacity in an exercise duration-dependent manner. Here, we investigated the effects of acute endurance exercise of different intensities on mouse pancreatic amylase activity. Male C57BL/6J mice performed low- or high-intensity running exercise for 60 min at either 10 (Ex-Low group) or 20 m/min (Ex-High group). The control group comprised sedentary mice. Immediately after acute exercise, pancreatic amylase activity was significantly decreased in the Ex-High group and not the Ex-Low group in comparison with the control group. To determine whether the decreased amylase activity induced by high-intensity exercise influenced muscle glycogen recovery after exercise, we investigated the rates of muscle glycogen resynthesis in Ex-High group mice administered either oral glucose or starch solution (2.0 mg/g body weight) immediately after exercise. The starch-fed mice exhibited significantly lower post-exercise glycogen accumulation rates in the 2-h recovery period compared with the glucose-fed mice. This difference in the glycogen accumulation rate was absent for starch- and glucose-fed mice in the sedentary (no exercise) control group. Furthermore, the plasma glucose AUC during early post-exercise recovery (0-60 min) was significantly lower in the starch-fed mice than in the glucose-fed mice. Thus, our findings suggest that acute endurance exercise diminishes the carbohydrate digestive capacity of the pancreas in a manner dependent on exercise intensity, with polysaccharides leading to delayed muscle glycogen recovery after exercise.


Asunto(s)
Glucógeno , Ratones Endogámicos C57BL , Músculo Esquelético , Condicionamiento Físico Animal , Animales , Masculino , Glucógeno/metabolismo , Condicionamiento Físico Animal/fisiología , Músculo Esquelético/metabolismo , Amilasas/metabolismo , Ratones , Glucemia/metabolismo , Almidón/metabolismo , Glucosa/metabolismo , Resistencia Física/fisiología , Carrera/fisiología , Páncreas/enzimología , Páncreas/metabolismo , Carbohidratos de la Dieta/administración & dosificación , Carbohidratos de la Dieta/metabolismo
17.
J Anim Sci ; 1022024 Jan 03.
Artículo en Inglés | MEDLINE | ID: mdl-38581217

RESUMEN

Pelleted total mixed ration (P-TMR) feeding, which has become a common practice in providing nutrition for fattening sheep, requires careful consideration of the balance between forage neutral detergent fiber (FNDF) and rumen degradable starch (RDS) to maintain proper rumen functions. The present study aimed to investigate the effects of the dietary FNDF/RDS ratio (FRR) on chewing activity, ruminal fermentation, ruminal microbes, and nutrient digestibility in Hu sheep fed a P-TMR diet. This study utilized eight ruminally cannulated male Hu sheep, following a 4 × 4 Latin square design with 31 d each period. Diets consisted of four FRR levels: 1.0 (high FNDF/RDS ratio, HFRR), 0.8 (middle high FNDF/RDS ratio, MHFRR), 0.6 (middle low FNDF/RDS ratio, MLFRR), and 0.4 (low FNDF/RDS ratio, LFRR). Reducing the dietary FRR levels resulted in a linear decrease in ruminal minimum pH and mean pH, while linearly increasing the duration and area of pH below 5.8 and 5.6, as well as the acidosis index. Sheep in the HFRR and MHFRR groups did not experience subacute ruminal acidosis (SARA), whereas sheep in another two groups did. The concentration of total volatile fatty acid and the molar ratios of propionate and valerate, as well as the concentrate of lactate in the rumen linearly increased with reducing dietary FRR, while the molar ratio of acetate and acetate to propionate ratio linearly decreased. The degradability of NDF and ADF for alfalfa hay has a quadratic response with reducing the dietary FRR. The apparent digestibility of dry matter, organic matter, neutral detergent fiber, and acid detergent fiber linearly decreased when the dietary FRR was reduced. In addition, reducing the dietary FRR caused a linear decrease in OTUs, Chao1, and Ace index of ruminal microflora. Reducing FRR in the diet increased the percentage of reads assigned as Firmicutes, but it decreased the percentage of reads assigned as Bacteroidetes in the rumen. At genus level, the percentage of reads assigned as Prevotella, Ruminococcus, Succinivibrio, and Butyrivibrio linearly decreased when the dietary FRR was reduced. The results of this study demonstrate that the dietary FRR of 0.8 is crucial in preventing the onset of SARA and promotes an enhanced richness of ruminal microbes and also improves fiber digestibility, which is a recommended dietary FRR reference when formulating P-TMR diets for sheep.


Forage neutral detergent fiber (FNDF) and rumen degradable starch (RDS) are key components of carbohydrates in the diet for ruminants, which would reflect saliva secretion and the acid production potential of feed. However, appropriate FNDF to RDS ratios (FRR) applicable to ruminants under the condition of pelleted total mixed ration (P-TMR) feeding have not been reported. In this study, we investigated the effects of the dietary FRR on chewing activity, ruminal fermentation, ruminal microbial communities, and nutrient digestibility of Hu sheep under P-TMR feeding. The results indicate that reducing dietary FRR levels would induce acidosis in sheep, which negatively affected fiber utilization and ruminal bacterial communities. The FRR of 0.8 was a recommended dietary FRR when formulating a P-TMR diet for fattening sheep, as indicated by decreased ruminal acidosis risk and increased richness of ruminal microbes in the rumen as well as nutrient digestibility.


Asunto(s)
Acidosis , Enfermedades de las Ovejas , Masculino , Femenino , Animales , Ovinos , Leche/metabolismo , Masticación/fisiología , Almidón/metabolismo , Lactancia/fisiología , Detergentes/metabolismo , Ensilaje/análisis , Propionatos/metabolismo , Fermentación , Rumen/metabolismo , Fibras de la Dieta/metabolismo , Carbohidratos de la Dieta/metabolismo , Dieta/veterinaria , Nutrientes , Acetatos/metabolismo , Acidosis/veterinaria , Digestión/fisiología
18.
J Clin Invest ; 134(9)2024 Mar 21.
Artículo en Inglés | MEDLINE | ID: mdl-38512401

RESUMEN

Bacterial translocation from the gut microbiota is a source of sepsis in susceptible patients. Previous work suggests that overgrowth of gut pathobionts, including Klebsiella pneumoniae, increases the risk of disseminated infection. Our data from a human dietary intervention study found that, in the absence of fiber, K. pneumoniae bloomed during microbiota recovery from antibiotic treatment. We thus hypothesized that dietary nutrients directly support or suppress colonization of this gut pathobiont in the microbiota. Consistent with our study in humans, complex carbohydrates in dietary fiber suppressed the colonization of K. pneumoniae and allowed for recovery of competing commensals in mouse models. In contrast, through ex vivo and in vivo modeling, we identified simple carbohydrates as a limiting resource for K. pneumoniae in the gut. As proof of principle, supplementation with lactulose, a nonabsorbed simple carbohydrate and an FDA-approved therapy, increased colonization of K. pneumoniae. Disruption of the intestinal epithelium led to dissemination of K. pneumoniae into the bloodstream and liver, which was prevented by dietary fiber. Our results show that dietary simple and complex carbohydrates were critical not only in the regulation of pathobiont colonization but also disseminated infection, suggesting that targeted dietary interventions may offer a preventative strategy in high-risk patients.


Asunto(s)
Carbohidratos de la Dieta , Microbioma Gastrointestinal , Infecciones por Klebsiella , Klebsiella pneumoniae , Klebsiella pneumoniae/metabolismo , Humanos , Ratones , Animales , Infecciones por Klebsiella/microbiología , Infecciones por Klebsiella/prevención & control , Carbohidratos de la Dieta/metabolismo , Femenino , Masculino , Fibras de la Dieta/metabolismo , Mucosa Intestinal/microbiología , Mucosa Intestinal/metabolismo , Intestinos/microbiología
19.
Trop Anim Health Prod ; 56(2): 104, 2024 Mar 14.
Artículo en Inglés | MEDLINE | ID: mdl-38483713

RESUMEN

We investigated the effects of replacing ground corn with full-fat corn germ (FFCG) on milk production, milk composition, and nutrient use in cows fed sugarcane bagasse and cactus cladodes. Ten multiparous Girolando cows (average body weight 500 ± 66 kg, 90 ± 15 days in milk) were distributed in a replicated 5 × 5 Latin Square and assigned to five dietary treatments containing 0%, 25%, 50%, 75%, or 100% of full-fat corn germ in substitution to ground corn. Full-fat corn germ increased fat-corrected milk yield by 2.2 kg/day and the synthesis of fat, lactose, and total solids in milk by 94.4, 60.0, and 201.10 g/day, respectively (p < 0.05). Cows fed corn germ quadratically increased (p < 0.05) dry matter intake by 1.01 kg/day, with the intake of crude protein and total digestible nutrients following the same pattern. Conversely, the substitution of corn for full-fat corn germ linearly reduced (p < 0.05) the total non-fiber carbohydrate intake from 5.79 to 4.40 kg/d. Except for ether extract and non-fiber carbohydrates, full-fat corn germ did not alter (p > 0.05) nutrient digestibility. Cows fed corn germ excreted less (p < 0.05) urea-N in milk and urine N. These results demonstrate that full-fat corn germ can partially replace ground corn to enhance the milk production efficiency of crossbred cows fed cactus cladodes and sugarcane bagasse. Furthermore, including sugarcane bagasse in FFCG-supplemented diets prevents milk fat depression in cows fed cactus cladodes.


Asunto(s)
Cactaceae , Saccharum , Femenino , Bovinos , Animales , Leche/metabolismo , Celulosa/metabolismo , Zea mays , Lactancia , Dieta/veterinaria , Carbohidratos de la Dieta/metabolismo , Digestión , Rumen/metabolismo , Ensilaje/análisis
20.
Arch Anim Nutr ; 78(1): 60-77, 2024 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-38488818

RESUMEN

This study was performed to investigate the nitrogen (N) and carbohydrate digestive traits of grazing heifers. The experiment was carried out at the Federal University of Lavras. The treatments were a Marandu palisadegrass (Urochloa brizantha [Syn, Brachiaria brizantha] Stapf. A. Rich. cv. Marandu) monoculture fertilised with 150 kg N/[ha ∙ year] (FP) or Marandu palisadegrass mixed pasture with forage peanut (MP). The pastures were grazed by six rumen-cannulated zebu heifers. A double cross-over design was used in four periods. Nutritive value, intake and apparent digestibility of forage, ruminal traits and kinetics and N balance were evaluated. Apparent total-tract digestibility of dry matter (DM) and neutral detergent fibre (NDF) were greater for FP than for MP. There was no effect in apparent total-tract digestibility of N. The estimated intestinal digestibility of nutrients was greater on MP than FP. Even though N intake and faecal N output were greater on MP than FP, there was no effect in urine N output. The N balance tended to be greater on MP than FP. The forage peanut, which contains condensed tannins, decreased ruminal fibre degradation, apparent digestibility and ruminal protein degradation, increased N flow from the rumen. Inclusion of forage peanut in the mixed pasture decreased the ruminal fibre degradability but increased N retention by the animals.


Asunto(s)
Alimentación Animal , Fenómenos Fisiológicos Nutricionales de los Animales , Dieta , Digestión , Rumen , Animales , Bovinos/fisiología , Digestión/fisiología , Alimentación Animal/análisis , Femenino , Dieta/veterinaria , Rumen/fisiología , Rumen/metabolismo , Nitrógeno/metabolismo , Proteínas en la Dieta/metabolismo , Estudios Cruzados , Fertilizantes/análisis , Valor Nutritivo , Arachis/química , Carbohidratos de la Dieta/análisis , Carbohidratos de la Dieta/administración & dosificación , Carbohidratos de la Dieta/metabolismo , Brachiaria/química , Brachiaria/fisiología , Poaceae/química
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA