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1.
J Vet Med Sci ; 86(8): 866-871, 2024 Aug 02.
Artículo en Inglés | MEDLINE | ID: mdl-38880612

RESUMEN

Coltiviruses, belonging to the genus Coltivirus within the family Spinareoviridae, are predominantly tick-borne viruses. Some of these species have been implicated in human diseases; however, their diversity, geographical distribution, and evolutionary dynamics remain inadequately. Therefore, this study was undertaken to explore the phylogenetic evolution of coltiviruses and related viruses. Our results revealed the detection of novel coltivirus-related sequences in adult female Haemaphysalis megaspinosa ticks collected from Kanagawa Prefecture, Japan. Molecular phylogenetic analysis revealed a close association between the sequences and the genome sequences of known coltivirus-related viruses, namely Qinghe tick reovirus and Fennes virus. The putative coltivirus-related virus was tentatively designated the Nakatsu tick virus. This study provides insights into the phylogenetic evolution of coltiviruses and related viruses.


Asunto(s)
Coltivirus , Ixodidae , Filogenia , Animales , Japón , Femenino , Ixodidae/virología , Coltivirus/genética , Coltivirus/aislamiento & purificación , Coltivirus/clasificación , Genoma Viral
2.
Nat Commun ; 15(1): 2284, 2024 Mar 13.
Artículo en Inglés | MEDLINE | ID: mdl-38480794

RESUMEN

Banna virus (BAV) is the prototype Seadornavirus, a class of reoviruses for which there has been little structural study. Here, we report atomic cryo-EM structures of three states of BAV virions-surrounded by 120 spikes (full virions), 60 spikes (partial virions), or no spikes (cores). BAV cores are double-layered particles similar to the cores of other non-turreted reoviruses, except for an additional protein component in the outer capsid shell, VP10. VP10 was identified to be a cementing protein that plays a pivotal role in the assembly of BAV virions by directly interacting with VP2 (inner capsid), VP8 (outer capsid), and VP4 (spike). Viral spikes (VP4/VP9 heterohexamers) are situated on top of VP10 molecules in full or partial virions. Asymmetrical electrostatic interactions between VP10 monomers and VP4 trimers are disrupted by high pH treatment, which is thus a simple way to produce BAV cores. Low pH treatment of BAV virions removes only the flexible receptor binding protein VP9 and triggers significant conformational changes in the membrane penetration protein VP4. BAV virions adopt distinct spatial organization of their surface proteins compared with other well-studied reoviruses, suggesting that BAV may have a unique mechanism of penetration of cellular endomembranes.


Asunto(s)
Coltivirus , Reoviridae , Coltivirus/metabolismo , Microscopía por Crioelectrón , Reoviridae/metabolismo , Proteínas de la Cápside/metabolismo , Virión/metabolismo
3.
Front Cell Infect Microbiol ; 13: 1283580, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-38035340

RESUMEN

Introduction: Banna virus (BAV), a potential pathogen that may cause human encephalitis, is the prototype species of genus Seadornaviru within the family Reoviridae, and has been isolated from a variety of blood-sucking insects and mammals in Asia. Methods: Culicoides, Mosquitoes, and Ticks were collected overnight in Yunnan, China, during 2016-2023 using light traps. Virus was isolated from these collected blood-sucking insects and grown using Aedes albopictus (C6/36) cells. Preliminary identification of the virus was performed by agarose gel electrophoresis (AGE). The full genome sequences of the BAVs were determined by full-length amplification of cDNAs (FLAC) and sequenced using next-generation sequencing. Results: In this study, 13 strains BAV were isolated from Culicoides, Mosquitoes and Ticks. Their viral genome consisted of 12 segments of double-stranded RNA (dsRNA), and with three distinct distribution patterns. Sequence analysis showed that Seg-5 of four strains (SJ_M46, SJ_M49, JC_M19-13 and JC_C24-13) has 435 bases nucleotide sequence insertions in their ORF compared to other BAVs, resulting in the length of Seg-5 up to 2128 nt. There are 34 bases sequence deletion in Seg-9 of 3 strains (WS_T06, MS_M166 and MS_M140). Comparison of the coding sequences of VP1, VP2, VP5, VP9 and VP12 of the 13 BAV strains, the results show that VP1, VP2 and VP12 are characterised by high levels of sequence conservation, while VP9 is highly variable, under great pressure to adapt and may be correlated with serotype. While also variable, VP5 appears to be under less adaptive pressure than VP9. Additionally, phylogenetic analysis indicates that the 13 BAV strains locate in the same evolutionary cluster as BAVs isolated from various blood-sucking insects, and are clustered according to geographical distribution. Conclusion: The data obtained herein would be beneficial for the surveillance of evolutionary characteristics of BAV in China and neighboring countries as well as extend the knowledge about its genomic diversity and geographic distribution.


Asunto(s)
Aedes , Ceratopogonidae , Coltivirus , Garrapatas , Animales , Aedes/genética , Ceratopogonidae/genética , China , Coltivirus/genética , Genoma Viral , Mamíferos/genética , Filogenia , Garrapatas/genética
4.
Viruses ; 14(5)2022 05 05.
Artículo en Inglés | MEDLINE | ID: mdl-35632713

RESUMEN

Sedoreoviridae is a family of viruses belonging to the order Reovirales and comprises six genera, two of which, Orbivirus and Seadornavirus, contain arboviruses that cause disease in humans and livestock. Areas such as Yunnan Province in southwestern China, have high arboviral activity due in part to warm and wet summers, which support high populations of biting flies such as mosquitoes and Culicoides. Three viral isolates previously obtained from Culicoides collected at cattle farms in Shizong County of Yunnan Province, China, between 2019 and 2020 were completely sequenced and identified as Banna virus (BAV) genotype A of Seadornavirus and serotypes 1 and 7 of epizootic hemorrhagic disease virus (EHDV) of Orbivirus. These results suggest that Culicoidestainanus and C. orientalis are potential vectors of BAV and EHDV, respectively, and represent the first association of a BAV with C. tainanus and of an arbovirus with C. orientalis. Analysis using VP9 generally agreed with the current groupings within this genus based on VP12, although the classification for some strains should be corrected. Furthermore, the placement of Kadipiro virus (KDV) and Liao ning virus (LNV) in Seadornavirus may need confirmation as phylogenetic analysis placed these viruses as sister to other species in the genus.


Asunto(s)
Arbovirus , Ceratopogonidae , Coltivirus , Virus de la Enfermedad Hemorrágica Epizoótica , Reoviridae , Animales , Arbovirus/genética , Bovinos , China , Coltivirus/genética , Mosquitos Vectores , Filogenia , Reoviridae/genética
5.
Arch Virol ; 167(4): 1221-1223, 2022 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-35277776

RESUMEN

Banna virus (BAV) is a typical arbovirus whose infection is associated with fever and viral encephalitis. The whole genome of BAV is composed of 12 RNA segments. BAVs, which have been divided into three genotypes (A, B, and C) based on phylogenetic analysis of segment 12 or segment 9, are currently undergoing rapid evolution. Recent studies have shown that BAV variation can exceed intraspecific limits and generate novel viruses. In the current study, a new BAV strain, named 113c5, was isolated from Culex tritaeniorhynchus and found to be a member of genotype A2 based on phylogenetic analysis of segments 9 and 12. The complete genome sequence of the new BAV strain described in the current study might contribute to the surveillance of evolutionary characteristics of BAVs.


Asunto(s)
Coltivirus , Culex , Virus , Animales , China , Coltivirus/genética , Genoma Viral , Filogenia , Virus/genética
6.
Virus Res ; 313: 198739, 2022 05.
Artículo en Inglés | MEDLINE | ID: mdl-35271887

RESUMEN

The risk for the emergence of novel viral zoonotic diseases in animals and humans in Uganda is high given its geographical location with high biodiversity. We aimed to identify and characterize viruses in 175 blood samples from cattle selected in Uganda using molecular approaches. We identified 8 viral species belonging to 4 families (Flaviviridae, Peribunyaviridae, Reoviridae and Rhabdoviridae) and 6 genera (Hepacivirus, Pestivirus, Orthobunyavirus, Coltivirus, Dinovernavirus and Ephemerovirus). Four viruses were highly divergent and tetantively named Zikole virus (Family: Flaviviridae), Zeboroti virus (Family: Reoviridae), Zebtine virus (Family: Rhabdoviridae) and Kokolu virus (Family: Rhabdoviridae). In addition, Bovine Hepacivirus, Obodhiang virus, Aedes pseudoscutellaris reovirus and Schmallenberg virus were identified for the first time in Ugandan cattle. We report 8 viral species belonging to 4 viral families including divergent ones in the blood of cattle in Uganda. Hence, cattle may be reservoir hosts for likely emergence of novel viruses with pathogenic potential to cause zoonotic diseases in different species with serious public health implications.


Asunto(s)
Enfermedades de los Bovinos , Coltivirus , Flaviviridae , Virus ARN , Reoviridae , Animales , Bovinos , Enfermedades de los Bovinos/epidemiología , Humanos , Filogenia , Virus ARN/genética , Reoviridae/genética , Uganda/epidemiología
7.
Infect Genet Evol ; 95: 105076, 2021 11.
Artículo en Inglés | MEDLINE | ID: mdl-34500093

RESUMEN

Recently, RNA viruses have gained a mammoth concern for causing various outbreaks, and due to pandemics, they are acquiring additional attention throughout the world. An emerging RNA as well as vector-borne Banna Virus (BAV) is a human pathogen resulting in encephalitis, fever, headache, muscle aches, and severe coma. Besides human, pathogenic BAV was also detected from pigs, cattle, ticks, midges, and mosquitoes in Indonesia, China, and Vietnam. Due to high mutation tendency and dearth of a species barrier, this virus will consider as a significant threat in the near future throughout the planet, particularly in Africa. Despite of severe human case fatalities in several countries, there are no specific therapeutics, available vaccines, and other preventive measures against BAV. Thus, to find out the effective therapeutics and preventive strategies are crying exigency. In the present study, a unique multi-epitope-based peptide vaccine candidate is constructed using bioinformatics' tools that efficiently instigate immune cells for generating BAV antibodies. The potential vaccine candidates were developed using both T and B -cell epitopes. UniprotKB database was used to retrieve of two outer proteins (VP9 and VP4), and homologous sequences of BAV taxid: 7763, 649,604, 77,763, and 8453 were searched by NCBI BLAST. These serotypes are the most closely associated with the disease. Then combining the best-selected epitopes in various combinations with different adjuvants, three distinct vaccine candidates were formed. The validity tests were performed for the screened vaccine candidate regarding stability, allergenicity, and antigenicity parameters. Moreover, molecular dynamic simulations of the selected vaccine with TLR-8 immune receptor confirmed the stability of the binding pose and showed a significant response to immune cells. Thus, the results established that the designed chimeric peptide vaccine could enhance the immune response against BAV.


Asunto(s)
Proteínas de la Cápside/genética , Coltivirus/inmunología , Epítopos de Linfocito B/inmunología , Epítopos de Linfocito T/inmunología , Vacunas Virales/inmunología , Biología Computacional , Vacunas de Subunidad/inmunología
8.
Sci Data ; 7(1): 342, 2020 10 13.
Artículo en Inglés | MEDLINE | ID: mdl-33051449

RESUMEN

Mosquito-borne viruses such as Zika virus, Japanese Encephalitis virus and Dengue virus present an increasing global health concern. However, in-depth knowledge of the distribution and diversity of mosquito-associated viruses and their related vectors remains limited, especially for China. To promote their understanding, we present the first comprehensive dataset of the distribution and diversity of these viruses and their related vectors in China (including Taiwan, Hong Kong and Macau). Data was drawn from peer-reviewed journal articles, conference papers and thesis publications in both English and Chinese. Geographical data on mosquito-associated viruses' occurrence and related mosquito vector species was extracted, and quality-control processes employed. This dataset contains 2,428 accounts of mosquito-associated viruses' and mosquito species geo-referenced occurrences at various administrative levels in China. The prevalent mosquito-associated virus includes Japanese encephalitis virus, Dengue virus, Banna virus and Culex flavivirus, whereas the abundant mosquito vectors are Culex tritaeryohynchus, Aedes albopictus and Culex pipiens pallens. This geographical dataset delivers a distribution and diversity outline of mosquito-associated viruses in China, and also applicable in various spatial and risk-assessment analysis.


Asunto(s)
Aedes/virología , Culex/virología , Mosquitos Vectores/virología , Distribución Animal , Animales , China , Coltivirus , Virus del Dengue , Virus de la Encefalitis Japonesa (Especie) , Flavivirus , Geografía , Virus Zika
9.
J Virol ; 93(13)2019 07 01.
Artículo en Inglés | MEDLINE | ID: mdl-30971476

RESUMEN

In 2011, ticks were collected from livestock following an outbreak of Crimean Congo hemorrhagic fever (CCHF) in Gujarat state, India. CCHF-negative Hyalomma anatolicum tick pools were passaged for virus isolation, and two virus isolates were obtained, designated Karyana virus (KARYV) and Kundal virus (KUNDV), respectively. Traditional reverse transcription-PCR (RT-PCR) identification of known viruses was unsuccessful, but a next-generation sequencing (NGS) approach identified KARYV and KUNDV as viruses in the Reoviridae family, Orbivirus and Coltivirus genera, respectively. Viral genomes were de novo assembled, yielding 10 complete segments of KARYV and 12 nearly complete segments of KUNDV. The VP1 gene of KARYV shared a most recent common ancestor with Wad Medani virus (WMV), strain Ar495, and based on nucleotide identity we demonstrate that it is a novel WMV strain. The VP1 segment of KUNDV shares a common ancestor with Colorado tick fever virus, Eyach virus, Tai Forest reovirus, and Tarumizu tick virus from the Coltivirus genus. Based on VP1, VP6, VP7, and VP12 nucleotide and amino acid identities, KUNDV is proposed to be a new species of Coltivirus Electron microscopy supported the classification of KARYV and KUNDV as reoviruses and identified replication morphology consistent with other orbi- and coltiviruses. The identification of novel tick-borne viruses carried by the CCHF vector is an important step in the characterization of their potential role in human and animal pathogenesis.IMPORTANCE Ticks and mosquitoes, as well Culicoides, can transmit viruses in the Reoviridae family. With the help of next-generation sequencing (NGS), previously unreported reoviruses such as equine encephalosis virus, Wad Medani virus (WMV), Kammavanpettai virus (KVPTV), and, with this report, KARYV and KUNDV have been discovered and characterized in India. The isolation of KUNDV and KARYV from Hyalomma anatolicum, which is a known vector for zoonotic pathogens, such as Crimean Congo hemorrhagic fever virus, Babesia, Theileria, and Anaplasma species, identifies arboviruses with the potential to transmit to humans. Characterization of KUNDV and KARYV isolated from Hyalomma ticks is critical for the development of specific serological and molecular assays that can be used to determine the association of these viruses with disease in humans and livestock.


Asunto(s)
Coltivirus/clasificación , Coltivirus/aislamiento & purificación , Virus de la Fiebre Hemorrágica de Crimea-Congo/aislamiento & purificación , Fiebre Hemorrágica de Crimea/complicaciones , Orbivirus/clasificación , Orbivirus/aislamiento & purificación , Filogenia , Garrapatas/virología , Animales , Chlorocebus aethiops , Coltivirus/genética , Culicidae/virología , Genoma Viral , Virus de la Fiebre Hemorrágica de Crimea-Congo/clasificación , Virus de la Fiebre Hemorrágica de Crimea-Congo/genética , Fiebre Hemorrágica de Crimea/epidemiología , Fiebre Hemorrágica de Crimea/virología , Secuenciación de Nucleótidos de Alto Rendimiento , Humanos , India , Mosquitos Vectores/virología , Orbivirus/genética , Reoviridae/clasificación , Reoviridae/genética , Reoviridae/aislamiento & purificación , Reoviridae/ultraestructura , Células Vero , Ensayo de Placa Viral , Proteínas Virales/genética
10.
Bioorg Chem ; 86: 15-27, 2019 05.
Artículo en Inglés | MEDLINE | ID: mdl-30684859

RESUMEN

Structure based virtual screening of two libraries containing 27,628 numbers of antiviral compounds was used to discover a few of the potent inhibitor molecules against Banna virus (BAV). Cross-docking studies with many common interfering proteins provided five of the highly selective inhibitor for BAV. Analyses of the leading molecules with ADME-Tox filtering tool and atomistic molecular dynamics simulation studies finally discovered a benzoxazolone derivative as one of the most promising molecules towards the highly selective inhibition of BAV. The theoretical calculations are also supported by the experimental evidences where the interactions between the hit ligand and a model peptide sequence, mimicking the VP9 protein of BAV, were studied. Overall the development of a personalized therapeutic towards the highly selective inhibition of BAV is discussed herein for the first time in literature.


Asunto(s)
Antivirales/farmacología , Proteínas de la Cápside/antagonistas & inhibidores , Coltivirus/efectos de los fármacos , Descubrimiento de Drogas , Simulación de Dinámica Molecular , Bibliotecas de Moléculas Pequeñas/farmacología , Antivirales/química , Proteínas de la Cápside/metabolismo , Coltivirus/metabolismo , Relación Dosis-Respuesta a Droga , Evaluación Preclínica de Medicamentos , Ligandos , Pruebas de Sensibilidad Microbiana , Estructura Molecular , Bibliotecas de Moléculas Pequeñas/química , Relación Estructura-Actividad
11.
Int J Infect Dis ; 78: 93-98, 2019 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-30395978

RESUMEN

OBJECTIVES: Banna virus (BAV) is classified in the genus Seadornavirus within the Reoviridae family and considered to be an emerging pathogen. We aimed to develop a rapid and simple molecular detection approach for all BAV subgroups in isothermal conditions. METHOD: A set of six specific primers was designed to target the segment 12 of BAV, and the reverse transcription-loop mediated isothermal amplification (RT-LAMP) assay was developed and compared with conventional RT-PCR method. RESULTS: The amplification of the RT-LAMP assay can be obtained within 40min at 65°C. The results from specificity showed that only target BAVs RNA including genotypes A, B and C were amplified and the assay demonstrated a sensitivity of 3.6×10-2PFU/mL, which was higher than conventional RT-PCR measurement. A good reliability for the assay was presented in the further evaluation for BAVs RNA from serial diluted BAV-spiked serum and 47 pools of field mosquito samples. CONCLUSIONS: Our findings present a rapid, sensitive and specific RT-LAMP assay that can be applied for BAV detection in clinical or field samples in the future.


Asunto(s)
Coltivirus/aislamiento & purificación , Técnicas de Amplificación de Ácido Nucleico/métodos , Aedes , Animales , Culex , Humanos , Reproducibilidad de los Resultados , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Transcripción Reversa
12.
Virus Res ; 258: 68-72, 2018 10 15.
Artículo en Inglés | MEDLINE | ID: mdl-30316801

RESUMEN

By using DNA Star, CUSP of EMBOSS, Codon W and IBM SPSS Statistics, nucleotide composition and codon usage pattern of 115 genes are researched in 37 BAVs. It shows that the composition of all genes prefers to AU, compared to CG, and for most of genes, the order is A, U, G and C in the virus. The ENC-values of the genes are slightly high which shows the weak codon bias, in which the codon bias of VP9 gene is the highest. The codon usage pattern of 12 different genes is different and related to their composition, their function or their host. For example, VP9 gene encoding viral spike protein in contact with different hosts spread dispersedly and VP1, VP2, VP3 and VP4 genes encoding the capsid protein are concentrated on first quadrant in correspondence analysis. The ENC of VP5 is correlated to GC3s in correlation analysis. The points of VP12 gene are tightly close to the expected curve in the ENC-plot analysis but GC12 is not related to GC3 in neutrality analysis. All analysis indicates the codon usage pattern of 12 genes is influenced by both natural selection and neutral mutation in a different extent in BAV. As a pathogen of viral encephalitis, compositional analysis and codon bias analysis of BAV can provide a theoretical basis for the disease control.


Asunto(s)
Codón , Coltivirus/genética , Genoma Viral , Proteínas Estructurales Virales/genética , Mutación , Nucleótidos/genética , Sistemas de Lectura Abierta
13.
Viruses ; 10(10)2018 10 11.
Artículo en Inglés | MEDLINE | ID: mdl-30314338

RESUMEN

Banna virus (BAV) is considered to be an emerging human pathogen that is transmitted by blood-sucking insects. BAV was isolated from various species of mosquitoes, midges, and livestock. It is widely distributed geographically, since it was identified in China, Vietnam, and Indonesia. Previously reported evolution studies of BAV indicated that BAV can be divided into two groups, including isolates from China and Vietnam clustered in group A, and Indonesian isolates in group B. In this study, we report the isolation of a new strain of BAV named HB14-71-01 from Anopheles sinensis mosquitoes from Hubei, China. An in vitro comparison study of the HB14-71-01 isolate and the group A BAV revealed differences based on observed cytopathic effect, plaque size, and viral growth rates. Additionally, the phylogenetic analysis indicated that the Hubei isolate belongs to a novel genotype of BAV and emerged nearly 105 years ago (95% highest posterior density (HPD): 35⁻434), unlike the two previously reported genotypes A and B. Our findings extend the knowledge about the genomic diversity and potential vectors/hosts of BAVs and will improve understanding of the relationships between genetic variation and pathogenicity.


Asunto(s)
Anopheles/virología , Coltivirus/aislamiento & purificación , Mosquitos Vectores/virología , Infecciones por Reoviridae/virología , Animales , Anopheles/fisiología , China , Coltivirus/clasificación , Coltivirus/genética , Humanos , Mosquitos Vectores/fisiología , Filogenia , Infecciones por Reoviridae/transmisión
14.
Virol J ; 15(1): 64, 2018 04 06.
Artículo en Inglés | MEDLINE | ID: mdl-29625620

RESUMEN

BACKGROUND: Kadipiro virus (KDV) belongs to the Reoviridae family, which consists of segmented, non-enveloped, double-stranded RNA viruses. It has previously been isolated from Culex, Anopheles, Armigeres and Aedes mosquitoes in Indonesia and China. Here, we describe the isolation and characterization of SDKL1625 from Anopheles sinensis mosquitoes in Shandong province, China. METHODS: In this study, we isolated Kadipiro virus in Aedes albopictus C6/36 cell culture and the complete genome sequencing was made by next generation sequencing. RESULTS: We isolated and characterized a Kadipiro virus from Anopheles sinensis mosquitoes in 2016 in Shandong province, China. Nucleotide and amino acid homology analysis of SDKL1625 showed higher levels of sequence identity with QTM27331 (Odonata, China, 2016) than with JKT-7075 (Culex fuscocephalus, Indonesia, 1981). The SDKL1625 has 86-97% amino acid identity with the JKT-7075, 88-99% amino acid identity with the QTM27331. Among the 12 fragments, VP1, VP2, VP4, VP6, VP7, VP9 and VP12 showed high amino acid identity (> 90%) and VP5 showed the lowest identity (86% and 88%). CONCLUSIONS: This is the first identification of KDV from mosquito in China. Virus morphology and genome organization were also determined, which will further enrich our understanding of the molecular biological characteristics of KDV and seadornaviruses.


Asunto(s)
Anopheles/virología , Coltivirus/clasificación , Coltivirus/genética , Animales , Línea Celular , China , Coltivirus/aislamiento & purificación , Coltivirus/ultraestructura , Genoma Viral , Genómica/métodos , Secuenciación de Nucleótidos de Alto Rendimiento , Insectos Vectores/virología , Filogenia , ARN Viral
15.
Sci China Life Sci ; 60(12): 1372-1382, 2017 12.
Artículo en Inglés | MEDLINE | ID: mdl-29143280

RESUMEN

In an investigation of blood-sucking insects and arboviruses, a virus (YN12243) was isolated from Culicoides samples collected in the Sino-Burmese border region of Yunnan Province, China. The virus caused cytopathic effect (CPE) in C6/36 cells and passaged stably. Polyacrylamide gel analysis showed that the genome of YN12243 was composed of 12 segments of double-stranded RNA (dsRNA), with a distribution pattern of 6-6. The nucleotide and amino acid sequences of the coding region (1‒12 segments) were 17,803 bp and 5,925 amino acids in length, respectively. The phylogenetic analysis of VP1 protein (RdRp) revealed that YN12243 belonged to genus Seadornavirus of family Reoviridae, and further analysis indicated that YN12243 belongs to the Banna virus (BAV) genotype A2. Additionally, YN12243 was located in the same evolutionary cluster as BAV strains isolated from different mosquito species, suggesting that the BAV isolated from Culicoides does not have species barriers. These results indicate that Culicoides can also be a vector for BAV. In view of the hematophagous habits of Culicoides on cattle, horses, deer, and other large animals, as well as the possibility of spreading and causing a variety of animal arboviral diseases, it is important to improve infection detection and monitor the BAV in large livestock.


Asunto(s)
Ceratopogonidae/virología , Coltivirus/clasificación , Coltivirus/genética , Genoma Viral/genética , Filogenia , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Línea Celular , Mapeo Cromosómico , Coltivirus/crecimiento & desarrollo , Coltivirus/aislamiento & purificación , Mosquitos Vectores/virología , Análisis de Secuencia de ADN , Ensayo de Placa Viral , Proteínas Virales/genética
16.
Virus Res ; 242: 131-140, 2017 10 15.
Artículo en Inglés | MEDLINE | ID: mdl-28964878

RESUMEN

During the course of tick-borne virus surveillance in Japan, three independent isolates of probably the same virus were obtained from three geographically distant populations of the hard tick Haemaphysalis flava. Genome analyses of the three isolates demonstrated that they were closely related but distinct strains of a novel virus, designated Tarumizu tick virus (TarTV), which has a genome of 12 double-stranded RNA segments. The development of the virus-induced cytopathic effects on BHK cells significantly varied according to virus strains. Ten out of 12 segments of TarTV appeared to encode putative orthologs or functional equivalents of viral proteins of Colorado tick fever virus (CTFV) and Eyach virus, suggesting that TarTV is the third member of the genus Coltivirus in the family Reoviridae. This was supported by the facts that the 5'- and 3'-terminal consensus sequences of coltivirus genomes were found also in TarTV genome, and segment 9 of TarTV had sequence and structural features that may mediate a stop codon read-through as observed in that of CTFV. However, segment 7 and 10 of TarTV had no significant sequence similarities to any other proteins of known coltiviruses. Electron microscopic analysis demonstrated that TarTV particle had a non-enveloped bilayer icosahedral structure, and viral inclusion bodies were formed in infected cells. TarTV could infect and replicate in several mammalian cell lines tested, but show no clinical symptoms in intracerebrally inoculated mice. Taken together, our findings provide new insights into genetic diversity and evolution of the genus Coltivirus.


Asunto(s)
Coltivirus/clasificación , Coltivirus/aislamiento & purificación , Ixodidae/virología , Animales , Cápside/ultraestructura , Células Cultivadas , Coltivirus/genética , Cricetinae , Genoma Viral , Cuerpos de Inclusión Viral/ultraestructura , Japón , Ratones , Microscopía Electrónica de Transmisión , Filogenia , Infecciones por Reoviridae/patología , Infecciones por Reoviridae/virología , Análisis de Secuencia de ADN , Homología de Secuencia , Virión/ultraestructura
17.
Virol J ; 14(1): 181, 2017 09 18.
Artículo en Inglés | MEDLINE | ID: mdl-28923111

RESUMEN

BACKGROUND: Zoonotic transmission events play a major role in the emergence of novel diseases. While such events are virtually impossible to predict, wildlife screening for potential emerging pathogens can be a first step. Driven by recent disease epidemics like severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), and Ebola, bats have gained special interest as reservoirs of emerging viruses. METHODS: As part of a bigger study investigating pathogens in African bats we screened animals for the presence of known and unknown viruses. RESULTS: We isolated and characterised a novel reovirus from blood of free-tailed bats (Chaereophon aloysiisabaudiae) captured in 2006 in Côte d'Ivoire. The virus showed closest relationship with two human pathogenic viruses, Colorado tick fever virus and Eyach virus, and was able to infect various human cell lines in vitro. CONCLUSION: The study shows the presence of a coltivirus-related virus in bats from Sub-Sahara Africa. Serological studies could help to assess its impact on humans or wildlife health.


Asunto(s)
Quirópteros/virología , Coltivirus/aislamiento & purificación , Coltivirus/fisiología , Animales , Células Cultivadas , Chlorocebus aethiops , Coltivirus/clasificación , Coltivirus/ultraestructura , Côte d'Ivoire , Genoma Viral , Humanos , Filogenia , ARN Viral , Infecciones por Reoviridae/transmisión , Infecciones por Reoviridae/virología , Análisis de Secuencia de ADN , Células Vero
18.
J Gen Virol ; 97(12): 3359-3367, 2016 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-27902331

RESUMEN

Viral nucleic acids present in the plasma of 498 Kenyan adults with unexplained fever were characterized by metagenomics analysis of 51 sample pools. The highest to lowest fraction of plasma pools was positive for parvovirus B19 (75 %), pegivirus C (GBV-C) (67 %), alpha anellovirus (59 %), gamma anellovirus (55 %), beta anellovirus (41 %), dengue virus genotype 2 (DENV-2) (16 %), human immunodeficiency virus type 1 (6 %), human herpesvirus 6 (6 %), HBV (4 %), rotavirus (4 %), hepatitis B virus (4 %), rhinovirus C (2 %), Merkel cell polyomavirus (MCPyV; 2 %) and Kadipiro virus (2 %). Ranking by overall percentage of viral reads yielded similar results. Characterization of viral nucleic acids in the plasma of a febrile East African population showed a high frequency of parvovirus B19 and DENV infections and detected a reovirus (Kadipiro virus) previously reported only in Asian Culex mosquitoes, providing a baseline to compare with future virome studies to detect emerging viruses in this region.


Asunto(s)
Coltivirus/aislamiento & purificación , ADN Viral/sangre , Fiebre/virología , Parvovirus B19 Humano/aislamiento & purificación , Virosis/virología , Virus/aislamiento & purificación , Adulto , Coltivirus/clasificación , Coltivirus/genética , ADN Viral/genética , Femenino , Fiebre/sangre , Humanos , Kenia , Masculino , Parvovirus B19 Humano/clasificación , Parvovirus B19 Humano/genética , Filogenia , Virosis/sangre , Virus/clasificación , Virus/genética , Adulto Joven
19.
Infect Genet Evol ; 45: 250-255, 2016 11.
Artículo en Inglés | MEDLINE | ID: mdl-27590713

RESUMEN

Banna virus (BAV) is an emerging pathogen that causes human viral encephalitis and has been isolated from types of blood-sucking insects and mammals in Asia. However, there are no reported systematic studies that describe the origin and evolution of BAV. Here, a phylogenetic analysis of BAVs isolated from a variety of potential vectors and vertebrate hosts worldwide revealed that BAVs emerged in the beginning of the 20th century and do not exhibit a species barrier. The mean substitution rate of BAVs was 2.467×10-2substitution/site/year (95% HPD, 1.093×10-3 to 5.628×10-2). The lineage is mainly composed of BAVs from high-latitude regions, which are the most recently emerged viruses with significantly higher substitution rates compared with the lineage comprised of the isolates from middle or low-latitude regions. The genetic differences between BAV strains are positively correlated with the geographic distribution. Strains from the same latitude regions are almost 100% identical, whereas the differences between strains from long distance regions with different latitudes could be >60%. Our results demonstrate that BAV is an emerging virus at a stage that involves rapid evolution and has great potential for introduction into non-endemic areas. Thus, enhanced surveillance of BAV is highly recommended worldwide.


Asunto(s)
Coltivirus/clasificación , Coltivirus/genética , Enfermedades Transmisibles Emergentes/virología , Encefalitis por Arbovirus/virología , Animales , Evolución Molecular , Humanos , Filogenia , ARN Viral/análisis , ARN Viral/genética
20.
Zhonghua Liu Xing Bing Xue Za Zhi ; 37(9): 1277-1282, 2016 Sep 10.
Artículo en Chino | MEDLINE | ID: mdl-27655578

RESUMEN

Objective: To understand the evolution characteristics of Banna viruses (BAVs) isolated worldwide from 1980 to 2012. Methods: In this study, a phylogenetic analysis using Bayesian Markov Chain Monte Carlo simulations was conducted on all available 12th segment of genes of BAVs isolated worldwide from 1980 to 2012 to investigate the evolutionary and epidemiologic dynamics of BAVs. Results: The Bayesian phylogenetic analysis of BAVs revealed that the common ancestor of BAVs appeared 315 (95%HPD: 63-619) years ago. The evolutionary rate of BAV based on the 12th segment gene was estimated to be 2.33×10-3 (95%HPD: 2.84× 10-4-8.52×10-3) substitution per site per year, indicating BAV belong to an emerging arbovirus with rapid evolution. Conclusion: The evolution of emerging BAVs is rapid and the distribution of BAVs has expanded with new variant being detected, so it is necessary to enhance the surveillance to fully understand the natural distribution and pathogenicity of BAVs.


Asunto(s)
Coltivirus/genética , Filogenia , Teorema de Bayes , Coltivirus/patogenicidad , Evolución Molecular , Humanos , Cadenas de Markov , Método de Montecarlo
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